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Compound microorganism preparation

A technology of composite microorganisms and microorganisms, applied in the field of soil remediation, can solve problems such as soil pollution, achieve the effects of increasing transmission volume, good repair effect, and improved permeability

Inactive Publication Date: 2017-12-22
杭州更蓝生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Inorganic pollutants mainly include heavy metal elements such as cadmium, lead, and arsenic; in addition, excessive application of chemical fertilizers, pesticides, and solid waste in the cultivation process have caused soil pollution to varying degrees.

Method used

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  • Compound microorganism preparation
  • Compound microorganism preparation
  • Compound microorganism preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1: Enrichment of microbial flora degrading benzopyrene

[0029] The soil samples come from areas polluted by polycyclic aromatic hydrocarbons, and the sampling method is carried out by the five-point sampling method, with 200g of samples taken at each point, and a total of 1000g of samples are taken, which are mixed for testing.

[0030] Prepare benzopyrene inorganic salt medium: prepare 10g / L benzopyrene solution with acetone, sterilize with a 0.25mm filter membrane, measure a certain amount of benzopyrene solution and place it in a sterilized Erlenmeyer flask until the acetone is volatilized Then add an appropriate amount of sterilized inorganic salt basal medium, so that the final concentration of benzopyrene is 1500 mg / L.

[0031] Take 6 triangular flasks, place 150ml of liquid inorganic salt medium containing benzopyrene in each triangular flask, take 5 of the above triangular flasks (A1-A5), add 1g of soil sample to each flask, and add sterilized 1g of wa...

Embodiment 2

[0034] Example 2: Isolation, identification and acquisition of microbial flora degrading benzopyrene

[0035] As can be seen from the results in Table 1, the A2 and A4 culture fluids contain the dominant flora that degrades benzopyrene. Take 0.1ml of the above-mentioned A2 and A4 culture fluids, dilute them in a certain ratio, and apply them to the inorganic salt solid containing benzopyrene. On the culture medium, single colonies with different characteristics were picked, purified and inoculated on the inorganic solid salt medium containing benzopyrene, cultured and selected with a faster growth rate, 16S rDNA was extracted by alkaline lysis method and 16S rDNA Sequence analysis (sequence analysis was completed by Shanghai Meiji Biomedical Technology Co., Ltd.), BLAST comparison was performed on the sequencing results, and 15 strains with high degradation of benzopyrene were obtained. The names of the strains are shown in Table 2.

[0036] Table 2 Names of benzopyrene-degrad...

Embodiment 3

[0051] Soil conditioner can break soil compaction, loosen soil, improve soil air permeability, reduce soil bulk density, promote soil microbial activity, enhance soil fertilizer and water penetration; improve soil, enhance crop disease resistance, increase crop yield, improve agricultural product quality, restore The original ecology of crops and other functions. The soil conditioner provided by the invention is mainly used to improve the permeability of the soil and increase the oxygen transmission amount in the soil, so as to quickly establish a healthy microbial population structure.

[0052] The composition of the compound microbial preparation provided by the invention comprises by weight

[0053] Component A (soil conditioner): 35 parts of oyster shell fine powder, 15 parts of straw biomass coke, 14 parts of phosphate rock powder, 25 parts of ammoniated sludge, 0.1-0.2 parts of decomposing agent, 3-6 parts of gypsum powder;

[0054] Component B (microbial mixture): 0.1 ...

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Abstract

The invention provides a composite microbial preparation, which belongs to the technical field of soil remediation, and in particular relates to a composite microbial preparation for remediating polycyclic aromatic hydrocarbon-contaminated soil. The microbial preparation in the present invention contains microorganisms of different strains, and has dual functions of repairing the soil ecosystem and degrading polycyclic aromatic hydrocarbons. The composite microbial preparation provided by the invention also includes a soil conditioner, which can improve the permeability of the soil and increase the oxygen transmission in the soil, thereby quickly establishing a healthy microbial population structure. In addition, the compound microbial preparation of the present invention is a dry powder, which is easy to store and activate, and has a long shelf life. Microbial strains treat the soil without secondary pollution, and the action conditions are mild.

Description

technical field [0001] The invention belongs to the technical field of soil remediation, and in particular relates to a composite microbial preparation for remediating polycyclic aromatic hydrocarbons polluted soil. Background technique [0002] According to the "National Soil Pollution Survey Bulletin" jointly issued by the Ministry of Environmental Protection and the Ministry of Land and Resources in 2014, the total soil pollution rate in the country is 16.1%, and the rate of soil points in cultivated land is as high as 19.4%. Among land use and industrial waste sites, the exceeding standards were as high as 36.3% and 34.9% respectively. Among them, organic pollutants mainly include HCH, DDT, polycyclic aromatic hydrocarbons, and wastes from chemical plants, oil production areas, industrial areas, nitrogen fertilizer plants, electronics plants, coking plants, and pesticide plants. Inorganic pollutants mainly include heavy metal elements such as cadmium, lead, and arsenic;...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K17/40B09C1/10C09K101/00
CPCC09K17/40B09C1/10C09K2101/00
Inventor 董秋月
Owner 杭州更蓝生物科技有限公司
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