90 results about "Oxidized Glutathione" patented technology

The invention relates to the field of pharmaceutical synthesis and discloses a linaclotide synthesis method. The linaclotide synthesis method includes: performing solid-phase synthesis to obtain linaclotide resin with an N terminal, a Thr side chain, a Cys side chain, an Asn side chain, a Tyr side chain and a Glu side chain of an amino acid sequence shown in SEQ ID NO:1 coupled with protecting groups and with a C terminal coupled with a resin solid-phase carrier, cracking to remove the protecting groups and the resin solid-phase carrier prior to carrying out oxidizing reaction by the aid of a GSH (glutathione) / GSSH (oxidized glutathione) oxidization system to obtain a crude linaclotide product, and purifying the crude linaclotide product so that linaclotide is obtained. By the method, an Mmt protecting group is used for protecting a cysteine side chain, crude linear linaclotide peptide is synthesized by a one-by-one coupling mode, and the linaclotide is obtained by oxidization by the aid of the GSH / GSSH oxidization system. Compared with existing methods, the linaclotide synthesis method has the advantages that purity of the crude linear peptide is improved, the oxidization step can be performed without purification, and purity and yield of the crude linaclotide product are remarkably improved.

Methods of modulating, adjusting, and maintenance of the glutathione redox status of an individual, or cell, tissues, bodily fluids, or body compartments of the individual, are disclosed, as are compositions suitable for such modulating, adjusting, and maintenance. The modulation or adjustment is achieved by altering the amounts of reduced glutathione versus oxidized glutathione in the individual, or cells, tissues, bodily fluids, or body compartments of the individual. Modulation, adjustment, and maintenance of the redox status of an individual enables treatment, prevention or suppression of diseases or symptoms associated with diseases. These methods are achieved by using quinone derivatives.

The present invention relates to a kit for the treatment of cancer comprising (a) a container for containing a first compound (i) or a precursor thereof, said first compound or precursor being a compound that oxidizes glutathione (GSH); (b) a container for containing a second compound (ii) or a precursor thereof, said second compound or precursor being a compound that forms an adduct or conjugate with GSH; (c) a container for containing a third compound (iii) or a precursor thereof, said third compound or precursor being a compound that inhibits the rate-limiting enzyme of GSH biosynthesis, gamma-glutamylcysteine synthetase (GCS); and (d) a container for containing a fourth compound (iv) or a precursor thereof, said fourth compound or precursor being a compound that inhibits the enzyme responsible for the conversion of GSSG to GSH, glutathione reductase (GR).

The invention provides methods, compositions and kits relating to hyaluronan based matrices using oxidized glutathione as a crosslinking agent.

The present invention relates to a composite for the treatment of a variety of medical conditions, the composite comprising an oxidized glutathione-based compound, which has a disulfide bond, and a metal material, in particular where the metal is either platinum or palladium. The oxidized glutathione-based compound and metal material can be present in a ratio of 3000 to 1 and preferably 1000 to 1. The oxidized glutathione-based compound can be oxidized glutathione itself or salts or derivatives. A feature of the invention is that the composite has a more stabilized disulfide bond than the oxidized glutathione-based compound itself. Methods for preparing the composite are provided, such methods being beneficial in that the composite is provided in high yields and at high purity. Methods for treating various medical conditions with the composites of the present invention are also disclosed.

The present invention is directed to a method for decreasing inflammation and oxidative stress in a mammal comprising; administration to a mammal a composition comprising a glucose anti-metabolite; and wherein said composition comprises amounts of the glucose anti-metabolite sufficient to decrease a level of an oxidized glutathione and / or increase the ration of reduced glutathione to oxidized glutathione in the blood of the mammal subsequent to administration of the glucose anti-metabolite.

The present invention generally relates to pharmacology and, in particular, to the treatment of tumors and other conditions. In some aspects, the invention is directed to the treatment of subjects having tumors or cancers that are metastatic. Surprisingly, it has been found that certain compositions comprising oxidized glutathione-based compounds are able to effectively treat such cancers by inhibiting cell migration and / or invasion processes, and thus, inhibiting tumor cell metastases. Without being bound by any theory, it is believed that such compositions are effective since the compositions are able to suppress the activation of critical signaling pathways within cells that are used for cell migration, such as the ErbB2 and / or phosphoinositide-3 kinase (PI3K) pathways, including the downstream RhoA and AKT pathways. Such pathways are regulated by ERp5, which is a protein disulfide isomerase regulated using certain redox pathways, and those redox pathways are unusually sensitive to treatment using oxidized glutathione-based compounds. Thus, the composition of the instant invention, in some embodiments, are surprisingly effective at preventing tumor metastases. While other references have disclosed the treatment of cancers using oxidized glutathione-based compounds, no reference has suggested that signaling pathways used for cell migration, invasion and metastasis, such as the ErbB2, PI3K, RhoA, and AKT pathways, are highly susceptible to treatment by altering the redox state of the cell, e.g., by oxidized glutathione-based compounds. Accordingly, the use of such compositions to treat tumor metastases is surprising and could not be predicted given the teachings of the prior art.

Disclosed is an excellent process for producing reduced glutathione by the electrolytic reduction of oxidized glutathione. In a process for producing reduced glutathione by the electrolytic reduction of oxidized glutathione using a cathode vessel and an anode vessel separated from each other with a septum, reduced glutathione is produced using an aqueous oxidized glutathione solution containing an electrical conducting agent other than an acid and having a pH value of 2.0 to 3.0 in the cathode vessel.

The present invention relates to a composite for the treatment of a variety of medical conditions, the composite comprising an oxidized glutathione-based compound, which has a disulfide bond, and a metal material, in particular where the metal is either platinum or palladium. The oxidized glutathione-based compound and metal material can be present in a ratio of 3000 to 1 and preferably 1000 to 1. The oxidized glutathione-based compound can be oxidized glutathione itself or salts or derivatives. A feature of the invention is that the composite has a more stabilized disulfide bond than the oxidized glutathione-based compound itself. Methods for preparing the composite are provided, such methods being beneficial in that the composite is provided in high yields and at high purity. Methods for treating various medical conditions with the composites of the present invention are also disclosed.

A universal folding method that has been demonstrated to be effective in refolding a variety of very different proteins expressed in bacteria as inclusion bodies has been developed. Representative proteins that can be dissolved and refolded in biologically active form, with the native structure, are shown in Table I. The method has two key steps to unfold and then refold the proteins expressed in the inclusion bodies. The first step is to raise the pH of the protein solution in the presence of denaturing agents to pH greater than 9, preferably 10. The protein solution may be maintained at the elevated pH for a period of up to about 24 hours, or the pH immediately decreased slowly, in increments of about 0.2 pH units / 24 hours, until the solution reaches a pH of about 8.0, or both steps used. In the preferred embodiment, purified inclusion bodies are dissolved in 8 M urea, 0.1 M Tris, 1 mM glycine, 1 mM EDTA, 10 mM beta-mercaptoethanol, 10 mM dithiothreitol (DTT), 1 mM reduced glutathione (GSH), 0.1 mM oxidized glutathione (GSSG), pH 10. The absorbance at 280 nm (OD280) of the protein solution is 5.0. This solution is rapidly diluted into 20 volumes of 20 mM Tris base. The resulting solution is adjusted to pH 9.0 with 1 M HCl and is kept at 4° C. for 24 hr. The pH is adjusted to pH 8.8 and the solution is kept at 4° C. for another 24 hrs. This process is repeated until the pH is adjusted to 8.0. After 24 hr at pH 8.0, the refolded proteins can be concentrated by ultrafiltration and applied to a gel filtration column for purification.

The present invention relates to a composite for the treatment of a variety of medical conditions, the composite comprising an oxidized glutathione-based compound, which has a disulfide bond, and a metal material, in particular where the metal is either platinum or palladium. The oxidized glutathione-based compound and metal material can be present in a ratio of 3000 to 1 and preferably 1000 to 1. The oxidized glutathione-based compound can be oxidized glutathione itself or salts or derivatives. A feature of the invention is that the composite has a more stabilized disulfide bond than the oxidized glutathione-based compound itself. Methods for preparing the composite are provided, such methods being beneficial in that the composite is provided in high yields and at high purity. Methods for treating various medical conditions with the composites of the present invention are also disclosed.

The invention relates to a method for producing an intelligent degradable edible film by using pea starch. The method comprises the following steps: S1, component blending, namely adding 100-120kg ofstarch into 1000L of purified water under stirring, carrying out dispersion and uniform mixing, then, carrying out further stirring for 10-15min, then, adding 40-60L of plasticizer, carrying out stirring for 10-15min, then, adding 12-18kg of fortifier, 1-1.5kg of anhydrous calcium chloride, 500-800g of plant extracts, 500-800g of natural colorant and 3-5kg of oxidized glutathione, and carrying outstirring for 30-40min; S2, homogeneous degasification; S3, tape casting; S4, drying; and S5, film uncovering, mechanical film winding, packaging and storage. The film material disclosed by the invention is capable of realizing identification of quality change of a food through color development and has good antibacterial properties, mechanical properties, oil resistance, humidity resistance and oxygen resistance. The method can be applied to packaging of various materials such as foods, health products, medicines and feeds.

The invention provides a composition for preventing or treating infectious diseases of a virus belonging to the Coronavirus family or Flavivirus family, the composition containing one or more substances selected from reduced glutathione or oxidized glutathione or pharmaceutically acceptable salts thereof and catechin, and a composition for preventing or treating infectious diseases of a virus belonging to the Coronavirus family or Flavivirus family, the composition containing reduced glutathione or oxidized glutathione or pharmaceutically acceptable salts thereof and catechin.

The invention provides a renaturation liquid for recombining a chicken alpha interferon inclusion body as well as a preparation method and an application thereof. The renaturation liquid mainly comprises arginine, EDTA (Ethylene Diamine Tetraacetic Acid), oxidized glutathione, Tris and glycerol, wherein the concentration of each component is as follows: 0.2-0.8 M of arginine, 0.5-2 mM of EDTA, 0.3-0.9 mM of toxidized glutathione, 60-100 mM of Tris and 15-30% (v / v) of glycerol; and the pH (Potential of Hydrogen) of the renaturation liquid is 8.0-8.5. The renaturation liquid is prepared by dissolving in distilled water and utilizing HCl to adjust the pH value; the renaturation liquid has an application prospect on the aspect of recombining the chicken alpha interferon inclusion body; and the renaturation liquid has a reasonable proportion for components to carry out a three-step washing and purifying and drip sample injection primary renaturation process method on the inclusion body to better separate a bacterial protein with the inclusion body, so that the renaturation rate of the protein is greatly improved.

The invention discloses a glycine-containing feed additive and applications thereof. The invention aims to protect a feed additive taking glycine as an effective component and following applications 1)-5) of glycine or the feed additive: 1) an application of improving the growth performance of weaned piglets; 2) an application of increasing the content of free glycine in the plasma of the weaned piglets; 3) an application of increasing the content of reduced glutathione in the plasma of the weaned piglets; 4) an application of reducing the contents of glutamine, ammonia, urea and oxidized glutathione in the plasma of the weaned piglets; and 5) an application of reducing the ratio of oxidized glutathione to reduced glutathione in the plasma of the weaned piglets. By adding glycine in the daily ration, the average daily feed intake, average daily gain and feed conversion efficiency of weaned piglets of 8-15kg can be obviously increased. Tests show that by adding 0.75% or 1.5% of glycine into the corn-bean meal daily ration for the weaned piglets of 8-15kg, the best growth performance of the weaned piglets can be favorably exerted.

Provided is a novel crystalline oxidized glutathione hexahydrate. Crystalline oxidized glutathione hexahydrate is extracted by cooling an aqueous solution containing oxidized glutathione to 15 DEG C or less, producing crystalline oxidized glutathione hexahydrate.

The composition, in accordance with the present invention, for producing a plant body having an improved sugar content includes glutathione, a polynucleotide encoding γ-glutamylcysteine synthetase, or a polynucleotide encoding glutathione-binding plastid type fructose-1,6-bisphosphate aldolase. The composition preferably includes oxidized glutathione. This allows provision of a composition for easily producing a plant body having an improved sugar content.

The invention discloses a cleaning liquid after chemical mechanical polishing and a preparation method thereof. The cleaning liquid comprises the following raw material components in mass fraction: 0.01%-25% of alkali, 0.01%-30% of alcohol amine, 0.001%-1% of an antioxidant, 0.01%-0.1% of polypeptide, 0.01%-0.1% of amino acid, 0.01%-10% of a corrosion inhibitor, 0.01%-10% of a chelating agent, 0.01%-5% of a surfactant, 28.9-89.9% of water, based on 100% in total, wherein the amino acid is a combination of histidine and cysteine, and the polypeptide is reduced glutathione (peptide A) and oxidized glutathione (peptide B). The cleaning liquid is stronger in cleaning ability, is lower in corrosion rate, is stronger in BTA removal ability, is better in stability, and can realize the effects of cleaning, corrosion inhibition and BTA removal at the same time.

The invention relates to a traditional Chinese herbal medicine preparation for heat stress resistance for aquatic animals and a preparation method thereof. The traditional Chinese herbal medicine preparation is prepared from the following raw materials in parts by weight through processing: 20 to 30 parts of traditional Chinese herbal medicine extracts, 10 to 30 parts of oyster extracts, 5 to 10 parts of oxidized glutathione and 40 to 60 parts of deionized water, wherein the traditional Chinese herbal medicine extracts are prepared by performing digestion on the following raw materials in parts by weight including 10 to 20 parts of camphor, 10 to 20 parts of rhizoma zingiberis, 10 to 20 parts of radix et rhizoma rhei, 10 to 20 parts of fructus foeniculi, 10 to 20 parts of cortex cinnamomi,10 to 20 parts of pepper and 10 to 20 parts of lotus leaves by water being 70 to 80 DEG C and performing concentration until the water content is 40 to 50 percent. Various kinds of medicine of the traditional Chinese medicine extracts are matched for simultaneously achieving the effects of strengthening the spleen, relieving heat, performing cooling and relieving summer-heat; oyster enzymatic hydrolysate and oxidized glutathione are used as auxiliary materials; the oxidized glutathione and inherent reducing type glutathione in tissue cells achieve the complementary cooperated effects to realize the efficient anti-oxidization function; the tissue cells of the aquatic animals are healthy and have high activity; the absorption utilization rate on the medicine is high; the heat stress resistance is obvious.

The invention discloses a compounding method for glutathione and belongs to the technical field of cosmetics and medicines. The method comprises the following steps: protecting L-cystine with phthalicanhydride (called as phthalic anhydride for short); preparing acyl chloride and condensing acyl chloride with glycine protected with hexamethyldisilazane (called as HMDS for short), thereby acquiringcysteine glycine dipeptide protected with phthalic anhydride; removing the phthalic anhydride protecting group, thereby acquiring the cysteine glycine dipeptide; protecting glutamic acid with phthalic anhydride and then reacting with acetic anhydride, thereby acquiring anhydride anhydride; reacting with cysteine glycine dipeptide, thereby acquiring tripeptide; and removing phthalic anhydride protection, thereby acquiring oxidized glutathione, and then reducing S-S bond, thereby acquiring the reduced glutathione GSH. According to the method, a chemical synthesis method is adopted for synthesizing the oxidized and reduced glutathione, the raw materials are low in cost, the yield is high, the high-purity products can be acquired and the method can meet the requirement for industrial production.

The present invention provides crystals of glutathione oxidized n hydrate (wherein n is an integer or a fraction of not less than 0 and less than 8) useful, for example, as final products of, as raw materials for, or as intermediates of health foods, pharmaceuticals, cosmetics or the like, and a process for producing crystals of glutathione oxidized, which comprises a step of crystallizing glutathione oxidized from a mixture of an aqueous solution containing glutathione oxidized and a water-miscible organic solvent and which is suitable for large-scale synthesis or industrialization.

A shoot of a plant is cultivated under the presence of glutathione, so that the shoot is rooted. It is possible to cultivate the shoot under the presence of glutathione by use of a rooting medium including glutathione or by contacting a solution including glutathione with the shoot. Oxidized glutathione is preferably used as glutathione. By promoting rooting of the shoot of the plant, a rooting rate of the shoot of the plant is improved. This improves productivity of a clone seedling.

The invention discloses an application of a cleaning fluid after chemical mechanical polishing. The invention specifically discloses the application of the cleaning fluid to cleaning of a semiconductor device after chemical mechanical polishing. The raw materials of the cleaning fluid comprise the following components by mass: 0.01%-25% of strong base, 0.01%-30% of alcohol amine, 0.001%-1% of antioxidants, 0.01%-0.1% of polypeptide, 0.01%-0.1% of amino acid, 0.01%-10% of corrosion inhibitors, 0.01%-10% of chelating agents, 0.01%-5% of surfactants and 28.9%-89.9% of water, and the sum of the mass fractions of all the components is 100%, wherein the amino acid is a combination of histidine and cysteine, and the polypeptide is a combination of reduced glutathione (peptide A) and oxidized glutathione (peptide B). The cleaning fluid is higher in cleaning capacity, lower in corrosion rate, higher in BTA removal capacity and higher in stability, and the effects of cleaning, corrosion inhibition and BTA removal can be achieved at the same time.

The present invention addresses the problem of providing a method for producing oxidized glutathione (GSSG) and oxidized gamma-glutamylcysteine, which is a precursor thereof, by a simple process. This method for producing GSSG includes, as means for addressing the problem described above, a step A' for reacting L-cysteine and L-glutamic acid to generate oxidized gamma-glutamylcysteine, and a step B' for reacting oxidized gamma-glutamylcysteine and glycine to generate GSSG.

The invention discloses a method for screening glutathione reductase inhibitors based on fluorescence recovery of carbon dots. Yellow fluorescence carbon dots for Ag<+> ion specific quenching serve asa fluorescence probe; glutathione reductase can perform catalytic reduction on oxidized glutathione to produce reduced glutathione in the presence of a coenzyme NADPH (Nicotinamide Adenine Dinucleotide Phosphate), and the reduced glutathione can perform competitive binding with the Ag<+> ion so as to further recover fluorescence of the carbon dots; and the glutathione reductase treated and inactivated by the glutathione reductase inhibitor cannot perform catalytic reduction on the oxidized glutathione, therefore, the fluorescence of the carbon dots cannot be recovered. On this basis, screening of the glutathione reductase inhibitors can be realized by virtue of the fluorescence recovery situation. The method for screening glutathione reductase inhibitors disclosed by the invention has thecharacteristics of being high in sensitivity, excellent in selectivity, low in cost, low in toxicity, environment-friendly, high in stability and the like, and has significances for screening relateddrugs based on the glutathione reductase inhibitor.

The invention relates to a method for detecting reduced glutathione (GSH) and / or oxidized glutathione (GSSG) based on a surface enhanced Raman spectroscopy, and also relates to a kit for detecting GSHand / or GSSG. The detection method and kit are simple in operation, high in detection speed and high in sensitivity, and can be well used for determining the content of GSH and GSSG in an alkylating agent infected cell sample, thereby rapidly screening and detecting an alkylating agent.