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Process for production of reduced glutathione

A technology of oxidized glutathione and glutathione, applied in reduction electrolysis, peptide, electrolytic organic production, etc., can solve the problem of incomplete production method of reduced glutathione

Active Publication Date: 2012-11-28
KYOWA HAKKO BIO CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Therefore, the production method of reduced glutathione by conventional electrolytic reduction is not entirely a realistic method in terms of production efficiency and equipment

Method used

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  • Process for production of reduced glutathione
  • Process for production of reduced glutathione
  • Process for production of reduced glutathione

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preparation example Construction

[0034] The preparation method of the supersaturated solution of oxidized glutathione is not limited, and can be mentioned: including a method of dissolving oxidized glutathione in an alkaline solution at a high concentration and desalting, including oxidizing glutathione A method of adsorbing a peptide to an ion exchange resin and eluting it in a solution having a high concentration includes a method of concentrating a solution having a concentration not exceeding saturation solubility, and the like.

[0035] The solution used in the anode cell in the present invention is not particularly limited as long as it is a conductive aqueous solution, and there may be mentioned: inorganic acid solutions such as hydrochloric acid and sulfuric acid; organic acid solutions such as acetic acid and propionic acid; agent solution, etc. Regarding the concentration of inorganic acid and organic acid, the conductivity is low at low concentration, and the ion exchange membrane is easily deterio...

Embodiment 1

[0043] pH Stability of Reduced Glutathione

[0044] Using reduced glutathione aqueous solution (100 g / L, pH 2.90) and sulfuric acid, reduced glutathione aqueous solutions (100 g / L) adjusted to pH 0.6, 1.2 and 2.0 were produced.

[0045] Each solution was stored at 25° C. for 24 to 36 hours, and the residual amount of reduced glutathione was quantified by high performance liquid chromatography (HPLC) under the following conditions. Such as Figure 1A As shown, it was found that the solution below pH 2.0 had a lower residual rate of reduced glutathione than the solution at pH 2.90. In addition, if Figure 1B As shown, it was found that solutions below pH 2.0 showed a higher increase in impurities due to hydrolysis compared to solutions at pH 2.90. Regarding impurities, the total amount of substances other than reduced glutathione and oxidized glutathione was quantified under the following HPLC conditions, and the concentration (g / L) converted to reduced glutathione was calcula...

Embodiment 2

[0053] Electrolytic Reduction of Oxidized Glutathione Using Sodium Sulfate as Conductive Agent

[0054] An aqueous solution of oxidized glutathione (about 400 g / L) was prepared by adding sodium hydroxide to adjust the pH to 7.0, which was passed through a cation exchange resin for desalination to prepare a supersaturated aqueous solution of oxidized glutathione ( about 350g / L). The solution was diluted, and sodium sulfate was added to prepare an aqueous oxidized glutathione solution (310 g / L) containing 0.75 mol / L sodium sulfate. The pH of the solution was 2.91.

[0055] The electrolyzer used contained anode side (150mL) and cathode side (300mL), and both passed 50cm 2 The cation exchange membrane SELEMION CMT (manufactured by Asahi Glass Co., Ltd.) separated. As an anode, use a 50cm 2 Platinized titanium plate, as cathode, use 50cm 2 zinc plate. The anode tank contained a 0.50 mol / L sulfuric acid solution (140 mL), and the cathode tank contained the oxidized glutathione...

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Abstract

Disclosed is an excellent process for producing reduced glutathione by the electrolytic reduction of oxidized glutathione. In a process for producing reduced glutathione by the electrolytic reduction of oxidized glutathione using a cathode vessel and an anode vessel separated from each other with a septum, reduced glutathione is produced using an aqueous oxidized glutathione solution containing an electrical conducting agent other than an acid and having a pH value of 2.0 to 3.0 in the cathode vessel.

Description

technical field [0001] The present invention relates to a method for producing reduced glutathione by electrolytic reduction of oxidized glutathione. Background technique [0002] As a method of electrolytic reduction of disulfide compounds, a method using an alloy composed of two or more specific metals as a cathode is known (Patent Document 1). In addition, as a method of producing L-cysteine ​​by electrolytic reduction of L-cystine, which is a kind of disulfide compound, a cation exchange membrane is used as a diaphragm and a mineral acid such as A method of acidifying an L-cystine solution with hydrochloric acid or the like is known (Patent Document 2). As a method of producing reduced glutathione by electrolytic reduction of oxidized glutathione, a method of using an aqueous solution of oxidized glutathione acidified by adding a mineral acid such as concentrated hydrochloric acid to the cathode side electrolytic cell has also been used. Known (Patent Document 3). [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C25B3/04C07K5/037C25B3/25
CPCC25B3/04C07K5/0215C25B3/25
Inventor 福本一成
Owner KYOWA HAKKO BIO CO LTD
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