The invention relates to the field of medicinal synthesis and discloses a method for synthesizing linaclotide. The method comprises the following steps: synthesizing a linaclotide resin coupled with protecting groups on side chains Thr, Cys, Asn, Tyr and Glu of an amino acid sequence shown as SEQ ID NO:1 and coupled with a resin carrier at the terminal C; performing acid hydrolysis to remove the protecting groups and resin carrier, obtaining a linaclotide linear crude peptide, cyclizing the linear crude peptide by adopting a cysteine / DMSO buffer solution, obtaining a crude linaclotide product, purifying and converting the crude product into acetate, thereby obtaining the finished product. According to the method disclosed by the invention, starting from a cyclizing system, the linaclotide synthesis method is improved, the total yield of linaclotide is improved by a stage by virtue of simple and rapid process steps, and the purity can reach a high level. Meanwhile, the later-stage cyclizing time is greatly shortened, and compared with the prior art, the method has high practical value and application prospects.