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Glutathione reductase determination kit, and preparation method and applications thereof

A technology of glutathione and oxidized glutathione, which is applied in biochemical equipment and methods, microbial determination/inspection, and measurement devices, can solve the problem of poor stability of kits, weak anti-interference ability, and linear range Small problems, to achieve a wide linear range, reduce self-degradation, improve the effect of the linear range

Active Publication Date: 2020-06-23
浙江达美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented technology improves upon existing methods that use enzymes or other chemicals to detect substances like drugs from blood samples. It allows for accurate analysis without causing damage caused by factors such as temperature fluctuations during testing. Additionally, this new method uses specific ingredients together to create an effective solution even at longer times while still maintaining its effectiveness over multiple tests.

Problems solved by technology

This patented technical problem addressed by this patent relates to improving the accuracy or sensitivity of measuring gluthosiderucleosynthesis reactions due to their low activities over several hours during hospitalization procedures. Current commercially available glutaroxin redox test systems require laborious extraction processes involving complicated operations like lyophilic acid precipitation and filtrations, which may result in wasted materials. Therefore, these current techniques cannot provide reliable measurement data without sacrificing valuable healthcare resources.

Method used

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  • Glutathione reductase determination kit, and preparation method and applications thereof
  • Glutathione reductase determination kit, and preparation method and applications thereof
  • Glutathione reductase determination kit, and preparation method and applications thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A glutathione reductase assay kit, including reagent R1 and reagent R2, wherein, reagent R1 includes: phosphate buffer 50mmol / L, ascorbate oxidase 1KU / L, pyruvate oxidase 1KU / L, ferrocyanide Potassium chloride 5mg / L, oxidized glutathione GSSG 2mmol / L, MgCl 2 1mmol / L, FAD 1μmol / L, sodium azide 0.1g / L and Brij-351mL / L. Reagent R2 includes: glycine buffer solution 50mmol / L, NADPH 0.1mmol / L, sodium azide 0.1g / L and protective agent 1g / L. Wherein, the protective agent is trehalose, dextran and glycerin with a weight ratio of 1:1:3.

Embodiment 2

[0035]A glutathione reductase assay kit, including reagent R1 and reagent R2, wherein, reagent R1 includes: phosphate buffer 500mmol / L, ascorbate oxidase 5KU / L, pyruvate oxidase 5KU / L, ferrocyanide Potassium chloride 20mg / L, oxidized glutathione GSSG 10mmol / L, MgCl 2 10mmol / L, FAD 20μmol / L, PC300 1g / L and TritonX-10010mL / L. Reagent R2 includes: glycine buffer solution 500mmol / L, NADPH 1mmol / L, gentamicin sulfate 1g / L and protective agent 10g / L. Wherein, the protective agent is trehalose, dextran and glycerin with a weight ratio of 9:1:10.

Embodiment 3

[0037] The difference from Example 1 is that the reagent R1 also includes EGTA 1mmol / L, and the reagent R2 also includes EGTA 1mmol / L.

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Abstract

The invention provides a glutathione reductase determination kit, and a preparation method and applications thereof, and relates to the technical field of biochemical reagent determination. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a phosphate buffer solution, ascorbic acid oxidase, pyruvate oxidase, potassium ferrocyanide, oxidized glutathione, MgCl2, FAD, a preservative and a surfactant; and the reagent R2 includes a glycine buffer solution, NADPH, the preservative and a protective agent. Through the adding of EGTA and dithiothreitol in the reagents, the linearrange of the kit can be enlarged, and the stability of the kit can be enhanced. The preparation method and applications of the kit are also provided; and the kit can accurately determine glutathione reductase.

Description

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Claims

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Application Information

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Owner 浙江达美生物技术有限公司
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