42results about How to "Improve the linear relationship" patented technology

A current mode dual-slope temperature-to-digital conversion device is disclosed. The conversion device comprises a temperature dependent current source and a reference current source. Firstly, a capacitor is charged by the temperature dependent current source. Next, the capacitor is discharged by the reference current source. The capacitor is coupled to at least one trigger, and the trigger sends out a first digital signal to a logic controller by the voltage of the capacitor. Then, the logic controller sends out a second digital signal to a time-to-digital converter according to the first digital signal. When the capacitor is discharged by the reference current source and before the first digital signal is varied, the converter receives the second digital signal and a clock signal to generate a corresponding digital output value. The present invention achieves the requirement of the high linearity resolution with the dual-slope architecture lest the curvature effect resulted from the time-domain circuit be occurred.

The invention provides a glutathione reductase determination kit, and a preparation method and applications thereof, and relates to the technical field of biochemical reagent determination. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a phosphate buffer solution, ascorbic acid oxidase, pyruvate oxidase, potassium ferrocyanide, oxidized glutathione, MgCl2, FAD, a preservative and a surfactant; and the reagent R2 includes a glycine buffer solution, NADPH, the preservative and a protective agent. Through the adding of EGTA and dithiothreitol in the reagents, the linearrange of the kit can be enlarged, and the stability of the kit can be enhanced. The preparation method and applications of the kit are also provided; and the kit can accurately determine glutathione reductase.

The invention provides a finished hybrid power automobile torque distribution system and method based on parallel control and relates to the field of hybrid power automobile control. The finished hybrid power automobile torque distribution system based on the parallel control consists of a real system and an artificial system, wherein the real system is responsible for driving of actual running of a real automobile, the artificial system is responsible for real automobile modeling and controller parameter optimization in a real automobile real-time running state and parallel execution. The real system consists of a sensor detection circuit, a signal conditioning circuit, a controller and a liquid crystal display. The artificial system consists of a sensor detecting circuit, a signal conditioning circuit and a computer processing system. The finished hybrid power automobile torque distribution system based on the parallel control is simple in structure, easy to achieve and high in reliability, improves the engine fuel utilization rate, reduces emission and effectively improves the actual running performance and working efficiency of the real automobile within a whole service life.

The invention belongs to the field of medicinal chemistry, and in particular relates to a LC-MSMS method for determining the content of methyl 5-isoquinolinesulfonate and ethyl 5-isoquinolinesulfonate in fasudil hydrochloride. HPLC solves the problem that methyl 5-isoquinolinesulfonate and ethyl 5-isoquinolinesulfonate cannot be simultaneously determined under single liquid phase conditions, and the mobile phase has great damage to the chromatographic column, and the chromatographic column is not durable, resulting in poor method reproducibility . LC‑MS has high detection sensitivity and can detect two components at the same time. The method has good durability, high accuracy, shortens analysis time and improves detection efficiency.

The invention discloses a method for high-precision detection of CEA by molecular imprinting combined with biomolecular Raman silent regional standard SERS technology, which firstly prepares gold-silver core-shell nanoparticles with internal standards and polydopamine-coated gold cores with labeled signals. Shell nanoparticles, and then assemble gold-silver core-shell nanoparticles on a gold plate as a SERS substrate, and prepare a molecularly imprinted layer containing CEA on the SERS substrate to obtain a SERS molecularly imprinted layer that can specifically recognize CEA. The CEA in the solution was specifically captured by CEA molecular imprinting, then the captured CEA was labeled with an antibody-modified SERS probe, and finally SERS detection was performed. The ratio of the signal intensities was used to estimate the concentration of CEA in the solution. The invention can greatly improve the detection efficiency of CEA, and realize high-precision and specific detection of trace amount of CEA.

The invention relates to a SERS system for simultaneously detecting multiple toxic substances, including the following substances A and B: substance A is an anti-fouling magnetic microbead coupled with two or more toxic substance haptens, expressed as MB@P‑hap , MB is a magnetic microbead, P is an antifouling polymer brush, and hap is a hapten of a toxic substance, which is obtained by coupling a toxic substance hapten to a magnetic microbead grafted with an antifouling polymer brush; B substance is two kinds of The above mixture of nanoprobes labeled with monoclonal antibodies of toxic substances, said nanoprobes labeled with monoclonal antibodies of toxic substances is represented as SERS substrate@mAb‑RP, wherein SERS substrate represents a surface-enhanced Raman scattering substrate, and mAb represents a toxic substance monoclonal antibody, RP means Raman probe (Raman Probe). The detection and sensing system constructed by the present invention is implemented in a sample solution based on competitive immunoassay, and has satisfactory detection accuracy and sensitivity, and can be used for detection of trace toxic substances in complex matrices such as food.

The invention relates to the technical field of drug analysis, and specifically discloses a detection method for genotoxic impurities in safinamide mesylate. Described detection method comprises the following steps: prepare need testing solution and reference substance solution; Adopt liquid chromatography-mass spectrometry to detect described need testing solution and described reference substance solution, the chromatographic condition of described liquid chromatography Be: adopt C18 chromatographic column, take the formic acid aqueous solution that volume concentration is 0.1% as mobile phase A, the formic acid methanol solution that volume concentration is 0.1% is mobile phase B, gradient elution; Described mass spectrometry adopts ESI ion source, positive ion detection mode. The detection method provided by the invention has the advantages of simple method, stability, high precision, good reproducibility, etc., can quickly and accurately detect the toxic impurities in the safinamide mesylate bulk drug, and conforms to the guiding principle of ICH M7 .

The invention discloses a method for constructing a SERS spectral probe to detect breast cancer marker EGFR phosphorylated tyrosine: a gold plate is used as a substrate, and core-shell structure nanoparticles are used as a substrate for SERS detection to develop high selectivity and high sensitivity A "sandwich" configuration probe molecule to detect the phosphorylation of breast cancer marker EGFR tyrosine. Using the 4‑MB Raman spectrum, the peak position in the Raman silent zone of biomolecules is 2221cm ‑1 The intensity of the SERS spectrum of the marker molecule was used as an internal standard to normalize the intensity of the spectral signal of the marker molecule 4-MBA, which can avoid the influence of the Raman signal in the biomolecular fingerprint region on the quantitative detection, and make the concentration of the EGFR phosphorylated tyrosine solution and the SERS intensity The linear relationship is greatly improved, further improving the detection sensitivity of the SERS spectral probe. 4-MB and 4-MBA are located in the middle layer between the gold core and the silver shell, which ensures that its properties, spatial structure and quantity remain unchanged, thereby ensuring the reliability of the data. The method of the present invention shows great potential in ultrasensitive detection of cancer markers.

The invention relates to a method for measuring the pH value of an aqueous solution based on gold nanorods, and belongs to the technical field of nanomaterials. The method comprises the following steps: (A) continuously monitoring the RGB color space values ​​of the solution after adding the gold nanorod solution and the etching solution to the solution to be tested, corresponding to the red, green and blue color component values ​​respectively, until the etching reaction ends; (B) Convert the GRB color space value into a hue value; (C) plot the hue value against the etching reaction time in the polar coordinate system, and obtain the reversal value of the hue within 400 seconds after the start of etching and the entire etching The maximum reverse value in the process; (D) When the reverse value is less than 38°, the maximum reverse value of the hue value is substituted into the calibration curve to realize the measurement of pH value. In the method provided by the invention, during the measurement process, the quality of the gold nanorods is screened through the inversion value, and unqualified colorimetric probes are eliminated, thereby significantly improving the measurement precision and accuracy.