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A glutathione reductase assay kit and its preparation method and application

A technology of glutathione and kits, which is applied in biochemical equipment and methods, microbial determination/inspection, measuring devices, etc., can solve the problems of poor stability of kits, weak anti-interference ability, and small linear range, etc. Achieve a wide linear range, reduce self-degradation, and improve the linear range

Active Publication Date: 2021-03-02
浙江达美生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the stability of the kit is relatively poor, and the anti-interference ability is not strong
[0006] In view of the poor stability, small linear range and weak anti-interference ability of existing glutathione reductase assay kits, it is necessary to find a kit with good stability, wide linear range and strong anti-interference ability. This kit enables the determination of glutathione reductase

Method used

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  • A glutathione reductase assay kit and its preparation method and application
  • A glutathione reductase assay kit and its preparation method and application
  • A glutathione reductase assay kit and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A glutathione reductase assay kit, including reagent R1 and reagent R2, wherein, reagent R1 includes: phosphate buffer 50mmol / L, ascorbate oxidase 1KU / L, pyruvate oxidase 1KU / L, ferrocyanide Potassium chloride 5mg / L, oxidized glutathione GSSG 2mmol / L, MgCl 2 1mmol / L, FAD 1μmol / L, sodium azide 0.1g / L and Brij-351mL / L. Reagent R2 includes: glycine buffer solution 50mmol / L, NADPH 0.1mmol / L, sodium azide 0.1g / L and protective agent 1g / L. Wherein, the protective agent is trehalose, dextran and glycerin with a weight ratio of 1:1:3.

Embodiment 2

[0035]A glutathione reductase assay kit, including reagent R1 and reagent R2, wherein, reagent R1 includes: phosphate buffer 500mmol / L, ascorbate oxidase 5KU / L, pyruvate oxidase 5KU / L, ferrocyanide Potassium chloride 20mg / L, oxidized glutathione GSSG 10mmol / L, MgCl 2 10mmol / L, FAD 20μmol / L, PC300 1g / L and TritonX-10010mL / L. Reagent R2 includes: glycine buffer solution 500mmol / L, NADPH 1mmol / L, gentamicin sulfate 1g / L and protective agent 10g / L. Wherein, the protective agent is trehalose, dextran and glycerin with a weight ratio of 9:1:10.

Embodiment 3

[0037] The difference from Example 1 is that the reagent R1 also includes EGTA 1mmol / L, and the reagent R2 also includes EGTA 1mmol / L.

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Abstract

The invention provides a glutathione reductase determination kit, and a preparation method and applications thereof, and relates to the technical field of biochemical reagent determination. The kit includes a reagent R1 and a reagent R2; the reagent R1 includes a phosphate buffer solution, ascorbic acid oxidase, pyruvate oxidase, potassium ferrocyanide, oxidized glutathione, MgCl2, FAD, a preservative and a surfactant; and the reagent R2 includes a glycine buffer solution, NADPH, the preservative and a protective agent. Through the adding of EGTA and dithiothreitol in the reagents, the linearrange of the kit can be enlarged, and the stability of the kit can be enhanced. The preparation method and applications of the kit are also provided; and the kit can accurately determine glutathione reductase.

Description

technical field [0001] The invention relates to the technical field of biochemical reagent assay, in particular to a glutathione reductase assay kit and its preparation method and application. Background technique [0002] Glutathione reductase (GR) is an enzyme that uses reduced NAD to catalyze the reaction of oxidized glutathione (GS-SG) into reduced form (GSH). It has extremely important physiological functions and is widely found in In serum, erythrocytes, mononuclear macrophages, heart, liver, kidney and other tissue cells, GR activity in whole blood of patients with various liver diseases and uremia will be significantly increased, while GR in tissues will be significantly reduced. GR activity can be used to assist in judging the liver and various diseases. For liver diseases, in the early stage of acute hepatitis, glutathione reductase in serum appears earlier than transaminase, and a comprehensive evaluation with the results of other examination items for acute hepa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/26
CPCC12Q1/26G01N2333/90203G01N2333/90212G01N2333/90235
Inventor 方朝君郑江萍丁佳金立方
Owner 浙江达美生物技术有限公司
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