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A SERS system for the simultaneous detection of multiple toxic substances

A technology of toxic substances and substances, which is applied in the field of SERS system, can solve the problems of full quantity, reliability, sensitivity and repeatability, which affect the detection accuracy, and the simultaneous on-site rapid detection method of multiple targets has not been reported, and achieves good results. Effect of extension potential, low cost, enhanced combining ability

Active Publication Date: 2021-10-19
BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] SERS is one of the multi-target detection methods in food and biological samples. It has simple technology, high sensitivity and strong coding ability, but there are still some obstacles in terms of fullness, reliability, sensitivity and repeatability.
And due to non-specific adsorption (fouling) and cross-reaction on the sensing interface, the coexistence of interference in the background matrix will affect its detection accuracy
At present, there are single immunoassay methods for DPN, BRD, and TET, but there are no reports on simultaneous on-site rapid detection methods for multiple targets

Method used

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  • A SERS system for the simultaneous detection of multiple toxic substances
  • A SERS system for the simultaneous detection of multiple toxic substances
  • A SERS system for the simultaneous detection of multiple toxic substances

Examples

Experimental program
Comparison scheme
Effect test

preparation example 1

[0074] Preparation Example 1 Chain transfer agent (CTA) trimethoxysilane trithiocarbonate

[0075] CTA according to literature (Qu, Z.; Hu, F.; Chen, K.; Duan, Z.; Gu, H.; Xu, H., J. Colloid and Interface Sci. 2013, 398, 82-87) reported method synthesis. Specifically, 1-propanethiol (6.6 mmol) was added to K 3 PO 4 (1.02 g, 6.6 mmol) in a stirred suspension of anhydrous acetone (15 mL), and stirred for about half an hour. Join CS 2 (1.1 mL, 18 mmol), the solution turned bright yellow. After stirring for another 10 min, (4-(chloromethyl)phenyl)-trimethoxysilane (1.43 mL, 6.6 mmol) was added, stirred for 13 h under a nitrogen atmosphere at room temperature, diluted with dichloromethane and filtered. After removal of the solvent from the filtrate under reduced pressure, purification by column chromatography on silica gel with a petroleum ether / ethyl acetate gradient gave a bright yellow oil, yielding the product ethyl trimethoxysilyltrithiocarbonate.

[0076] The syn...

preparation example 2MB

[0078] Preparation example 2 Preparation of MB@P-Cym, MB@P-CyM-hap and MB@hap

[0079] 1. Preparation of MB@P-Cym

[0080] Antifouling polymer brush-grafted magnetic beads (MB@P-CyM) were prepared by typical surface-initiated polymerization (Qu, Z.; Hu, F.; Chen, K.; Duan, Z.; Gu, H.; Xu, H., J. Colloid and Interface Sci. 2013, 398, 82-87.). The specific steps are: add 50 mg CTA to 100ml MBs of absolute ethanol (1.0 mg·mL -1 ) suspension, refluxed in nitrogen for 5 h, collected the obtained CTA-MBs, and washed 3 times with ethanol. CTA-MBs were suspended in ethanol for further use. CTA-MBs (0.1 g), AIBN (20 mg), and cysteine ​​methacrylate (CyM, 0.1 g) were dispersed in 10 mL of degassed water / methanol (1:1) solution for grafting CyM polymers brush. After bubbling nitrogen for 30 min, the system was closed and heated at 80°C. After 5 h of reaction, MB@P-CyM was collected and washed 3 times with DMF.

[0081] 2. Preparation of MB@P-CyM-hap

[0082] The triple hapt...

preparation example 3

[0092] Preparation example 3 Preparation of gold nanoflowers (AuNFs)

[0093] Prepared by hydrazine reduction method, specifically 2.0 mL of 50.0 mM HAuCl 4 Mix with 0.5 mL of 10.0 mM TA ethanol solution in 100 mL of deionized water. After stirring for 5 min, add 0.5 mL of fresh N 2 h 4 Quickly inject into solution. The color of the solution immediately changed to blue, indicating the formation of AuNFs. After standing at room temperature for 2 h, AuNFs were purified by ultracentrifugation (8 000 rpm, 5 min), washed twice with water, and finally dispersed in 10 mL of deionized water.

[0094] Figure 7 A is the TEM figure of the AuNFs obtained in Preparation Example 2, Figure 7 B is a TEM image of the AuNFs@PEG-mAb mixture obtained in Preparation Example 3. It can be seen that AuNFs are monodisperse with a size of 40–70 nm; while AuNFs@PEG-mAb can see a light-colored organic coating around gold nanoflowers (AuNFs). PEG can effectively enhance the stability of these...

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Abstract

The invention relates to a SERS system for simultaneously detecting multiple toxic substances, including the following substances A and B: substance A is an anti-fouling magnetic microbead coupled with two or more toxic substance haptens, expressed as MB@P‑hap , MB is a magnetic microbead, P is an antifouling polymer brush, and hap is a hapten of a toxic substance, which is obtained by coupling a toxic substance hapten to a magnetic microbead grafted with an antifouling polymer brush; B substance is two kinds of The above mixture of nanoprobes labeled with monoclonal antibodies of toxic substances, said nanoprobes labeled with monoclonal antibodies of toxic substances is represented as SERS substrate@mAb‑RP, wherein SERS substrate represents a surface-enhanced Raman scattering substrate, and mAb represents a toxic substance monoclonal antibody, RP means Raman probe (Raman Probe). The detection and sensing system constructed by the present invention is implemented in a sample solution based on competitive immunoassay, and has satisfactory detection accuracy and sensitivity, and can be used for detection of trace toxic substances in complex matrices such as food.

Description

technical field [0001] The invention relates to the field of SERS sensors, in particular to a SERS system for simultaneously detecting multiple toxic substances. Background technique [0002] Fast, accurate, and low-cost multi-target detection is of great significance in early diagnosis of diseases, food safety supervision, and environmental monitoring. However, current detection methods have limited sensitivity and weak multiplex detection ability, which limits their wide application. Surface-enhanced Raman spectroscopy (SERS) technology is simple, high-sensitivity, and strong coding ability. It is considered to be an ideal method for making multi-channel sensing platforms. There are some obstacles. In-depth understanding of interfacial chemistry to control the properties of target recognition and signal transduction is crucial for the development of high-performance sensing platforms. Currently, non-fouling polymer brushes have been used as sensing or binding interfaces...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/65B82Y15/00
CPCG01N21/658B82Y15/00
Inventor 孙洁芳邵兵张晶
Owner BEIJING CENT FOR DISEASE PREVENTION & CONTROL
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