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A method for high-precision detection of cea by molecular imprinting combined with silent regional standard sers technology

A surface molecular imprinting, CEA-SMIP-SERS technology, applied in the field of high-precision detection of CEA by SERS technology, can solve the problems affecting the final accuracy of quantitative detection, SERS peak intensity fluctuation, and limit further application, so as to improve detection sensitivity and ensure reliability Sexual, highly selective effect

Active Publication Date: 2022-07-12
FUJIAN NORMAL UNIV
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Problems solved by technology

However, although SMIP-SERS is sensitive and promising at present, it also has certain limitations: First, the Raman probe signal used by this method is located in the fingerprint region (300-1800 cm -1 ), so it is easily interfered by the Raman signal from endogenous biomolecules; secondly, the SERS peak intensity used for quantitative detection often fluctuates unexpectedly due to changes in laser power and external environment
Both of these factors will affect the final precision of quantitative detection, greatly limiting the further application of this method in the detection of complex biological samples.

Method used

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  • A method for high-precision detection of cea by molecular imprinting combined with silent regional standard sers technology
  • A method for high-precision detection of cea by molecular imprinting combined with silent regional standard sers technology
  • A method for high-precision detection of cea by molecular imprinting combined with silent regional standard sers technology

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Embodiment

[0026] 1. Reagent: chloroauric acid (HAuCl 4 ), sodium citrate (Na 3 C 6 H 5 O 7 ), phenylacetylene (EB), 4-p-mercaptobenzonitrile (MB), p-aminothiophenol (PATP), 4-mercaptophenylboronic acid (MPB), aminophenylboronic acid (APBA), ammonium persulfate (APS) , sodium dodecyl sulfate (SDS), ascorbic acid, silver nitrate (AgNO 3 ), phosphate buffered saline (PBS, pH=7.2 and 8.5), dopamine Tris hydrochloric acid buffer (pH=8.5), bovine serum albumin (BSA), glacial acetic acid (HAc), carcinoembryonic antigen (CEA) and antibodies ( Ab), gold plate (GCMS), glucose (Glucose), alpha-fetoprotein (AFP).

[0027] 2. Synthesis of Nanoparticles

[0028] 1) Synthesis of gold nanoparticles (Au NPs): add 99 mL of ultrapure water, 1 mL of 1% HAuCl to a conical flask 4 After stirring and boiling, add 1 mL of 1% sodium citrate solution, continue to stir and heat for 15 min, and cool at room temperature to obtain Au NPs sol.

[0029] 2) Synthesis of gold-silver core-shell nanoparticles (Au@...

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Abstract

The invention discloses a method for high-precision detection of CEA by molecular imprinting combined with biomolecular Raman silent regional standard SERS technology, which firstly prepares gold-silver core-shell nanoparticles with internal standards and polydopamine-coated gold cores with labeled signals. Shell nanoparticles, and then assemble gold-silver core-shell nanoparticles on a gold plate as a SERS substrate, and prepare a molecularly imprinted layer containing CEA on the SERS substrate to obtain a SERS molecularly imprinted layer that can specifically recognize CEA. The CEA in the solution was specifically captured by CEA molecular imprinting, then the captured CEA was labeled with an antibody-modified SERS probe, and finally SERS detection was performed. The ratio of the signal intensities was used to estimate the concentration of CEA in the solution. The invention can greatly improve the detection efficiency of CEA, and realize high-precision and specific detection of trace amount of CEA.

Description

technical field [0001] The invention belongs to the technical field of CEA analysis and detection, and in particular relates to a method for high-precision detection of CEA by using the SERS technology of molecular imprinting combined with the silent regional standard ratio. Background technique [0002] According to 2016 statistics, cancer has become the second leading cause of death in developed countries, with about 2.8 million people dying of cancer in China. Early screening of cancer and effective prognostic monitoring can greatly improve the five-year survival rate of patients, relying on ultrasensitive, reliable quantification of cancer biomarkers in tumor tissue and body fluid (blood, saliva, and urine) samples detection. Some of these cancer biomarkers have been widely used in clinical routine cancer screening. For example, carcinoembryonic antigen (CEA), one of the glycoproteins, as a traditional broad-spectrum tumor marker, has the ability to reflect the existen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/574G01N33/58G01N21/65
CPCG01N33/57473G01N33/58G01N21/658
Inventor 冯尚源林学亮林多卢玉栋
Owner FUJIAN NORMAL UNIV
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