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Low-temperature plasma degradation method for degrading polysaccharides into oligosaccharides

A low-temperature plasma and plasma technology, applied in the production of sugar, fructose, sugar production, etc., can solve the problems of not being a polysaccharide degradation method, damage to the health of production workers, and inability to achieve mass production, and achieve a good degradation effect. , The production cost is low, and the effect of eliminating safety hazards

Active Publication Date: 2017-06-13
DALIAN POLYTECHNIC UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production cost of the radiation method is relatively high, and it is harmful to the health of production workers. The ultrasonic method requires high pressure and high temperature conditions, and cannot be mass-produced, so it is still not an ideal polysaccharide degradation method.

Method used

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  • Low-temperature plasma degradation method for degrading polysaccharides into oligosaccharides

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Experimental program
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Effect test

Embodiment 1

[0033] Heparin is a polysaccharide, and this embodiment uses heparin as the polysaccharide raw material.

[0034] Prepare low-temperature plasma liquid with 10mL pure water as the medium: cover the lower electrode of the low-temperature plasma generator power supply with quartz, set the voltage to 10V, current to 3A, and discharge time to 10min. Take 10 mg of heparin, mix it with the prepared plasma liquid, stir for 10 min, and mix well. The mixed solution was collected for heating treatment, using silicone oil as the medium, heated in an oil bath at 160° C. for 10 minutes, and the degraded oligosaccharide solution was collected. Afterwards, the oligosaccharide solution is freeze-dried, and the obtained dry powder is the degraded oligosaccharide product.

[0035] The calculation method of the recovery rate is: the weight of the oligosaccharide product is divided by the weight of the polysaccharide before degradation. In this embodiment, 8.1 mg of the oligosaccharide product i...

Embodiment 2

[0038]Prepare low-temperature plasma liquid with 1mL 1% NaCl solution as the medium, cover the upper and lower electrodes of the low-temperature plasma generator with quartz, set the voltage to 1000V, current to 0.1A, and discharge time to 10s. Take 1 g of chondroitin sulfate, mix it with the plasma solution prepared above, stir for 1 second, mix evenly, collect and obtain the degraded oligosaccharide solution. The oligosaccharide solution was dialyzed for 24 hours, and then the oligosaccharide solution was freeze-dried, and the obtained dry powder was the degraded oligosaccharide product. In this example, 0.85 g of the oligosaccharide product was obtained, and the recovery rate reached 85%.

Embodiment 3

[0040] Prepare a low-temperature plasma liquid with 10mL of 10% KCl solution as a medium, cover the lower electrode of the low-temperature plasma with glass, set a voltage of 1000V, a current of 0.1A, and a discharge time of 10min. Take 1g of lentinan, mix it with the low-temperature plasma solution prepared above, stir for 10s, mix evenly, collect the mixed solution and heat it in an oil bath at 40°C for 10min, the collected solution is the oligosaccharide solution, ultrafilter the oligosaccharide solution for 24h, and then filter the oligosaccharide solution The sugar solution was freeze-dried, and the obtained dry powder was the degraded oligosaccharide product. In this example, 0.75 g of the oligosaccharide product was obtained, and the recovery rate reached 75%.

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Abstract

The invention discloses a low-temperature plasma degradation method for degrading polysaccharides into oligosaccharides. The method comprises the following steps: carrying out dry treatment or wet treatment on polysaccharides to obtain a plasma polysaccharide solution; heating the prepared plasma polysaccharide at 40-200 DEG C for 0 seconds-10 minutes to obtain an oligosaccharide solution; purification and recovery of degraded oligosaccharides: carrying out dialysis or ultrafiltration treatment on the oligosaccharide solution to implement desalting; and carrying out freeze-drying on the desalted oligosaccharide solution to obtain dry powder which is the oligosaccharides degraded from polysaccharides. When the low-temperature plasma technique is utilized to degrade the polysaccharides into oligosaccharides, pure water or saline water is used as the material to prepare the plasma, and the plasma is used as the degradation reagent for degrading the polysaccharides; and thus, the method has favorable degradation effects, and is safer since no other chemical aids are introduced. No pollutant is discharged in the production process, so that method satisfies the environmental standards of low carbon and environmental protection.

Description

technical field [0001] The invention relates to the technical field of polysaccharide degradation, in particular to a method for preparing oligosaccharides by degrading polysaccharides based on low-temperature plasma technology. Background technique [0002] As a recognized substance with good biological activity, polysaccharide has broad application and development prospects in the field of medicine. However, as a biological macromolecular polymer, polysaccharide has a large molecular weight, is difficult to absorb into the body, has a variety of molecular structures, and is difficult to control the production quality, which brings difficulties to drug control. Oligosaccharides have a variety of biological functions similar to polysaccharides. Its molecular structure is simple and clear, which is conducive to the absorption, utilization and metabolism of the human body. Its product quality is controllable, and it is beneficial to the evaluation and supervision of profession...

Claims

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Application Information

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IPC IPC(8): C13K1/00C13K5/00C13K7/00C13K11/00C13K13/00
CPCC13K1/00C13K5/00C13K7/00C13K11/00C13K13/00
Inventor 杨静峰宿黎刘文转衣萌赵君朱蓓薇
Owner DALIAN POLYTECHNIC UNIVERSITY
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