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Method for degrading high-molecular-weight polysaccharide containing alpha-1, 3-glycosidic bonds

A technology of large molecular weight and glycosidic bonds, which is applied in the field of degrading large molecular weight polysaccharides containing α-1,3-glycosidic bonds, can solve the problems of unsuitability for industrialized production expansion, product structure and activity destructiveness, and low degradation rate. Reduce platelet adhesion rate and blood viscosity, improve immune function, and have good effects on hypercholesterolemia

Active Publication Date: 2016-11-23
北京颐方生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the cleavage of glycosidic bonds in the acid degradation method is random, and the molecular weight distribution of the product is wide, which is destructive to the structure and activity of the product.
The hydrogen peroxide degradation method requires the use of hydrogen peroxide, which is potentially dangerous during storage and transportation, and has a low degradation rate, so it is not suitable for industrialized expansion of production

Method used

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  • Method for degrading high-molecular-weight polysaccharide containing alpha-1, 3-glycosidic bonds

Examples

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Embodiment 1

[0031] A kind of method that adopts electric Fenton method to degrade tremella polysaccharide: take by weighing 3000g tremella polysaccharide (molecular weight 560KDa), be dispersed in 3.0Kg dehydrated alcohol, join in 15L distilled water, make the sugar solution that concentration is 200mg / ml, pack into In an electrolytic cell with a volume of 24L, weigh 149.1g of sodium sulfate and dissolve it in the polysaccharide solution, adjust the pH to 3 with 1mol / L hydrochloric acid, use a 30cm×30cm wire mesh as the anode, and a 30cm×30cm porous graphite as the cathode, and the distance between the two electrodes is 5cm, connected to 12V DC, the current density is 12mA / cm 2, and use the aeration device to feed in purified air at a speed of 1.74L / min (or to feed in oxygen at a speed of 0.58L / min, the aeration device contains three air holes, and the three air holes are blown into oxygen at the same time), per liter The amount of air introduced into the Tremella polysaccharide solution ...

Embodiment 2

[0033] A kind of method that adopts electric Fenton method to degrade tremella polysaccharide: take by weighing 900g tremella polysaccharide (molecular weight 400KDa), be dispersed in 3.0Kg dehydrated alcohol, join in 15L distilled water, make the sugar solution that concentration is 60mg / ml, pack In an electrolytic cell with a volume of 24L, weigh 106.5g of sodium sulfate and dissolve it in the polysaccharide solution, adjust the pH to 3 with 1mol / L hydrochloric acid, use a 30cm×30cm wire mesh as the anode, and a 30cm×30cm porous graphite as the cathode, and the distance between the two electrodes is 8cm, connected to 10V DC, the current density is 10mA / cm 2 , and feed oxygen at a speed of 0.6L / min (the aeration device contains two vent holes, and the two vent holes are blown into oxygen at the same time), the amount of air per liter of tremella polysaccharide solution is about 4.0L, and the agitator Stir at 250rpm, electrolyze for 50 minutes at room temperature, stop power s...

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Abstract

The invention relates to a method for degrading high-molecular-weight polysaccharide containing alpha-1, 3-glycosidic bonds. The method includes: feeding a high-molecular-weight polysaccharide containing the alpha-1, 3-glycosidic bonds and of 60-200mg / mL in concentration into an electrolytic tank with a cathode and an anode; adjusting the solution to be acidic, feeding oxygen to the cathode according to an amount of 4.0-7.0 L oxygen / L polysaccharide solution, and feeding direct current and controlling current density of the cathode to be 10-12mA / cm2; degrading, and collecting electrolyte to obtain. The method has high selectivity to polysaccharide containing the alpha-1, 3-glycosidic bonds and is simple to operate, time-saving, efficient, mild in reaction condition, little to environment pollution and conducive to large-scale production. Importantly, compared with acid degradation and hydrogen peroxide degradation methods, the method has the advantages that an electric Fenton method is high in polysaccharide degradation rate, and obtained low-molecular-weight polysaccharide is narrower in molecular weight range.

Description

technical field [0001] The invention relates to the field of production of pharmaceutical raw materials, in particular to a method for degrading large molecular weight polysaccharides containing α-1,3-glucosidic bonds. Background technique [0002] Polysaccharide is formed by the condensation and dehydration of multiple monosaccharide molecules. It is a kind of carbohydrate with complex molecular structure and huge size. Active polysaccharide refers to a polysaccharide compound with special physiological activity, which has the function of regulating the circadian rhythm of the human body. Monosaccharides in polysaccharides are connected by glycosidic bonds, and glycosidic bonds include β-1,4-glycosidic bonds, α-1,3-glycosidic bonds, β-1,3-glycosidic bonds, etc. [0003] Studies have shown that the biological activity of polysaccharides is closely related to its molecular weight. The larger the molecular weight, the larger the molecular volume, and the poor solubility. lear...

Claims

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Application Information

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IPC IPC(8): C08B37/00C25B3/00A61K31/715A61P7/02A61P3/06A61P39/06
CPCA61K31/715C08B37/006C25B3/00
Inventor 石清东王姣
Owner 北京颐方生物科技有限公司
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