Preparation of ganoderma lucidum active polysaccharides by biofermentation degradation and analytical method of ganoderma lucidum active polysaccharides

Abstract

The invention discloses preparation of ganoderma lucidum active polysaccharides by biofermentation degradation and an analytical method of the ganoderma lucidum active polysaccharides. The analyticalmethod comprises the following step that S1, fermentation culture of ganoderma lucidum liquid is conducted, and bacterial strain of ganoderma lucidum is firstly transferred to a plate PDA medium in anincubator with 25-30DEG C for 5-7d for activation, after the plate is covered with mycelium, a fungus block with the size of 3-6mm<2> is picked out and put into a 250mL shaking bottle with the filling capacity of 100mL for liquid fermentation culture for 7-10d to obtain a first grade seed; and 10% inoculation dose is injected into a liquid medium. A yeast fermentation method for biodegradation ofganoderma lucidum macromolecular polysaccharides, combined with an ethanol precipitation method for preparation of active low molecular weight polysaccharide components, yeast added degrades the highmolecular weight polysaccharides in the extracellular fluid into lower molecular weight polysaccharides. By a fact that Dectin-1 receptor is combined to activate NF-[kappa]B, the ganoderma lucidum active polysaccharide components are used to enhance immune activity.

Description

Technical field [0001] The invention relates to the field of bioengineering, in particular to bio-fermentation degradation preparation of ganoderma lucidum active polysaccharide and its analysis method. Background technique [0002] Ganoderma spp. is a famous medicinal fungus in my country. It has a wide range of pharmacological effects and extremely low toxicity. It can be used clinically to assist in the treatment of various diseases, including tumors, bronchial inflammation, and neurasthenia. Modern pharmacological research has proved that the medicinal value of Ganoderma lucidum mainly includes immune regulation, anti-tumor, anti-radiation and auxiliary treatment of cardiovascular diseases. Ganoderma has gradually become an important resource in the field of research and development of health functional foods and traditional Chinese medicine products. [0003] The chemical components of Ganoderma lucidum are very complex. There are dozens of components that have been isolated....

AI Technical Summary

Problems solved by technology

The large molecular weight polysaccharide component has the characteristics of easy separation and good activity, but due to its large molecular weight, high viscosity and insoluble in water, its further development and application are greatly restricted

The traditional method uses acid-base hydrolysis, ultrasonic and microwave crushing to reduce the relative molecular weight of polysaccharides, so as to improve its water solubility and enhance the biological activity of polysaccharides, but it is difficult to popularize due to poor results and product safety issues.

In recent years, enzymatic biodegradation has been paid more and more attention, but polysaccharides are a kind of macromolecular substance with compact molecular structure, it is difficult to carry out enzymatic hydrolysis reaction inside the molecule, and the efficiency is low

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