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Method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves

A technology of golden camellia tea and golden camellia, applied in the field of biological extraction, can solve the problem of low immune activity, achieve the effects of enhancing immune activity, improving water solubility, and reducing molecular weight

Active Publication Date: 2015-12-23
GUILIN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above-mentioned literature discloses that Camellia polysaccharides have certain immune activity, the immune activity is not high.

Method used

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  • Method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1) Grinding the dried leaves of Camellia japonica, adding water 20 times its weight, and extracting twice by reflux, each extraction for 1 hour, combining the extracts, filtering, and the filtrate is the water extract to obtain the water extract;

[0022] 2) Concentrate the water extract to an extract with a relative density of 1.1 at 60°C, add 90v% ethanol until the alcohol content is 80v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0023] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 2 times of the weight of the precipitate, then add distilled water to dissolve, and the consumption of distilled water is 2 times of the precipitate weight after washing. times, and then add protease of 0.3% of the dry weight of golden camellia tea leaves, and enzymolysis time is 1 to 3 hours at 40°C to inactivate the enzyme to obtain an enzymo...

Embodiment 2

[0027] 1) Grinding the dried leaves of Camellia japonica, adding water 30 times its weight, reflux extraction 3 times, each extraction for 3 hours, combining the extracts, filtering, the filtrate is the water extract to obtain the water extract;

[0028] 2) Concentrate the water extract to an extract with a relative density of 1.3 at 60°C, add 95v% ethanol until the alcohol content in the mixed solution is 85v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0029] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 4 times of the precipitate weight, then add distilled water to dissolve, and the consumption of distilled water is 4 times of the precipitate weight after washing. times, and then add 3% protease of the dry weight of golden camellia tea leaves, enzymolysis time is 3h at 60°C, the enzyme is inactivated, and the enzymolysis solution...

Embodiment 3

[0033] 1) Pulverize the golden camellia tea leaves, add water 25 times its weight, reflux extraction 2 times, extract 2 hours each time, combine the extracts, filter, and the filtrate is the water extract to obtain the water extract;

[0034] 2) Concentrate the water extract to an extract with a relative density of 1.2 at 60°C, add 90v% ethanol until the alcohol content in the mixed solution is 80v%, let it stand for precipitation, suction filter, and take the precipitate for later use;

[0035] 3) the precipitate of step 2) is washed with dehydrated alcohol and acetone successively, and the consumption of dehydrated alcohol and acetone is 3 times of the precipitate weight, then add distilled water to dissolve, and the consumption of distilled water is 3 times of the precipitate weight after washing times, and then add 1.5% protease of the dry weight of golden camellia tea leaves, and enzymolysis time is 2 hours at 50°C to inactivate the enzyme to obtain the enzymolysis solutio...

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Abstract

The invention provides a method for extracting camellia chrysantha polysaccharide from camellia chrysantha leaves. The method comprises the following steps: 1, adding water to the camellia chrysantha leaves for conducting extraction, and obtaining an aqueous extract solution; 2, after concentrating the aqueous extract solution, adding ethanol for conducting precipitation, and obtaining precipitate for standby application; 3, washing the precipitate obtained in step 2 with absolute ethyl alcohol and acetone in sequence, then adding distilled water for conducting dissolution, then adding protease for conducting enzymolysis, and obtaining enzymatic hydrolysate; 4, placing the enzymatic hydrolysate and conducting treatment for 10-60 seconds under ultrasonic waves with the frequency ranging from 1000 kHz to 5000 kHz, then conducting dialysis through a dialysis bag with the molecular cut off being 10000, obtaining reserved liquid for conducting dialysis through a dialysis bag with the molecular cut off being 500000, obtaining penetrating liquid for conducting concentration, adding ethanol for conducting precipitation again, and obtaining precipitate for standby application; 5, washing the precipitate obtained in step 4 with absolute ethyl alcohol and acetone in sequence, then adding distilled water for conducting dissolution, conducting activated carbon decoloration, filtration and drying, and obtaining the camellia chrysantha polysaccharide. The camellia chrysantha polysaccharide obtained through the method has high immunological competence.

Description

technical field [0001] The invention belongs to the technical field of biological extraction, and in particular relates to a method for extracting polysaccharides from camellia japonica tea leaves. Background technique [0002] Camelliachrysantha (Hu)Tuyama belongs to the plant of Camellia genus Camellia in the family Theaceae, and is known as the "Queen of the Tea Clan" and "The Giant Panda in the Plant Kingdom". As a traditional Chinese herbal medicine of the Zhuang nationality in Guangxi, golden camellia tea has various physiological functions such as lowering blood sugar, lowering blood fat, inhibiting tumor growth, and improving the body's immunity. Among them, golden camellia polysaccharide is considered to be one of the important functional components. "Studies on Anti-immune Liver Injury Effects of Camellia Camellia Polysaccharides" (published in West China Pharmaceutical Journal, 2014, 9(5): 525-527) discloses that the extraction method of Camellia camellia polysacc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
Inventor 邹登峰张小玲肖胜军马硕陈素一
Owner GUILIN MEDICAL UNIVERSITY
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