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Preparation method for rhizoma bletillae polysaccharide

A polysaccharide and static technology, applied in the field of medicine, can solve problems such as poor color, high impurities, whiteness and low extraction rate of polysaccharides, and achieve the effect of ensuring clarity, high content, and broad application prospects

Inactive Publication Date: 2017-05-10
XIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a preparation method of bletilla polysaccharides, which solves the problems of low extraction rate, high impurities, and poor color of bletilla polysaccharides in the traditional method of decoction and alcohol precipitation, which cannot meet the needs of modern medical fields and in-depth research on new biological materials. Development Requirements Questions

Method used

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  • Preparation method for rhizoma bletillae polysaccharide

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preparation example Construction

[0021] The preparation method of Baiji Polysaccharide of the present invention specifically comprises the following steps:

[0022] Step 1: Add 8 to 15 times the amount of water to the decoction pieces of Baihe, soak for 60 to 90 minutes, heat immersion and extract at 80 to 100 ° C for 60 to 120 minutes, extract 3 times, and combine the 3 times of extracts. Refrigerate at ℃, let stand for 48h, separate the supernatant, discard the precipitation, and get the supernatant;

[0023] Step 2: Weigh the AB-8 macroporous adsorption resin with the same quality as the decoction pieces, add it into the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorption resin in it in a suspended state, and maintain stirring State for 2h, then filter and isolate AB-8 macroporous adsorption resin to obtain decolorized Baiji extract, which is for later use;

[0024] Step 3: vacuum-concentrate the decolorized Baiji extract obtained in step 2 at 70-85 ℃ to obt...

Embodiment 1

[0031] Step 1: Weigh 10 parts by mass of Baiji decoction pieces, add 8 times the amount of water, soak for 60 minutes, heat immersion and extract at 100 ° C for 60 minutes, extract 3 times, combine the three extracts, refrigerate at 4 ° C, Let stand for 48h, separate the supernatant, discard the precipitation, and obtain the supernatant;

[0032] Step 2: Weigh the AB-8 macroporous adsorption resin with the same quality as the decoction pieces, add it into the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorption resin in it in a suspended state, and maintain stirring State for 2h, then filter and isolate AB-8 macroporous adsorption resin to obtain decolorized Baiji extract, which is for later use;

[0033] Step 3: vacuum-concentrate the decolorized Baiji extract obtained in step 2 at 70°C to obtain a clear paste, put it at room temperature, add 95% ethanol to make the alcohol content reach 85%, refrigerate at 4°C, stand for 24h, F...

Embodiment 2

[0036] Step 1: Weigh 20 pieces of Baiji decoction pieces by weight, add 10 times the amount of water, soak for 80 minutes, heat immersion and extract at 90°C for 90 minutes, extract 3 times, combine the three extracts, refrigerate at 4°C, Let stand for 48h, separate the supernatant, discard the precipitation, and obtain the supernatant;

[0037] Step 2: Weigh the AB-8 macroporous adsorption resin with the same quality as the decoction pieces, add it into the supernatant obtained in step 1 for decolorization, stir to disperse the AB-8 macroporous adsorption resin in it in a suspended state, and maintain stirring State for 2h, then filter and isolate AB-8 macroporous adsorption resin to obtain decolorized Baiji extract, which is for later use;

[0038] Step 3: vacuum-concentrate the decolorized Baiji extract obtained in step 2 at 85 °C to obtain a clear paste, put it at room temperature, add 95% ethanol to make the alcohol content reach 75%, refrigerate at 4 °C, stand for 24h, ...

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Abstract

The invention discloses a preparation method for rhizoma bletillae polysaccharide. The preparation method comprises the following steps: first, weighing rhizoma bletillae decoction pieces, performing hot-dipping extracting after water is added for soaking, refrigerating, filtering, and performing vacuum concentrating after the extracting liquid is de-coloured by using AB-8 resin; then performing alcohol precipitation, refrigerating, filtering, removing ethyl alcohol by nitrogen flow, and freezing and drying to obtain white and dry rhizoma bletillae polysaccharide, wherein the content of the rhizoma bletillae polysaccharide is about 85 percent, and the water solubility is good. According to the preparation method, the rhizoma bletillae polysaccharide is extracted by adopting a hot water dipping method; rhizoma bletillae polysaccharide degradation and high impurity content caused by overheating when the rhizoma bletillae polysaccharide is extracted according to a water decocting method are avoided; the problem that the requirements of deep research and development of modern medical treatment fields and novel biological materials cannot be met because of low rhizoma bletillae polysaccharide extracting efficiency, high impurity, poor colour and lustre in the conventional water decocting and alcohol precipitating method is solved; the problem that the rhizoma bletillae polysaccharide is in short supply at present is also solved.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a preparation method of Baiji polysaccharide. Background technique [0002] Bletillata polysaccharide (BSP) is a water-soluble polymer viscous polysaccharide extracted from the traditional Chinese medicine Bletilla striata, also known as Bletillata glucomannan. It is composed of D-glucose and D-mannose according to approximately It is composed of a ratio of 1:4, and is polymerized by β-glycosidic bonds to form a spatial helical structure; its average molecular weight is 65,000 to 150,000 kDa, and it can be dissolved in water to form a viscous gel, which has good bioadhesion. It is extremely safe, and the toxicological safety evaluation shows that the polysaccharide of Baiji is safe, non-irritating and non-allergic. Therefore, it has a wide range of uses: ① It can be used as food ingredients or additives, and is an excellent natural food thickener. ②The polysaccharid...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003C08B37/006
Inventor 秦蓓杨宽
Owner XIAN MEDICAL UNIV
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