The invention relates to a shiitake cultivation medium. The shiitake cultivation medium is prepared through using the following raw materials, by weight, 30-40 parts of a bean straw particle, 30-40 parts of a Corchorus capsularis L stalk particle, 20-30 parts of husk, 10-20 parts of sugarcane leaf powder, 10-15 parts of walnut shell flour, 15-16 parts of corn flour, 8-10 parts of vinegar residue, 10-12 parts of bean dregs, 4-5 parts of Phaseolus radiatus L., 8-10 parts of fishbone meal, 1-2 parts of lactose, 0.3-0.5 parts of calcium carbonate, 2-3 parts of quicklime, 1-2 parts of gypsum powder, 0.5-1.0 part of calcium superphosphate, 0.2-0.3 parts of potassium dihydrogen phosphate and 5-10 parts of a sustained-release nutritional particle. The shiitake cultivation medium has the advantages of wide raw material sources, increase of the heat clearing and exterior syndrome relieving effects of shiitake cultivated through the medium, immunity improvement, and low raw material cost.