68results about How to "The cultivation process is simple" patented technology

The invention relates to a pholiota nameko culture method, which comprises the steps of: culture material proportioning and preparation, sterilization, inoculation and bagging, mycelium culture, summer-crossing management and primordium inducement fruiting. The pholiota nameko culture materials comprise the following recipe and mixture ratios: 30 to 50 parts of corncobs, 30 to 50 parts of soft mixed hardwood sawdust, 10 to 20 parts of bran, 2 to 4 parts of corn starch, 0.5 to 1.5 parts of gypsum and 0.5 to 1.5 parts of lime. The culture raw materials adopt the corncobs and the soft mixed hardwood sawdust as major materials, a good basis is laid for the smooth implementation of the Natural Forest Protection Projects and the pholiota nameko planting in flat areas, planting trays are omitted, the culture cost can be saved by 15 percent, the culture process is simple and is easy to implement, the work efficiency is improved, the edge fruiting effect of the pholiota nameko bag type culture is improved by 30 percent through being compared with that of the disc type culture, the edge fruiting effect of the edible fungi is sufficiently utilized, the yield can be improved by more than 10 percent, and the high-quality fruiting rate is improved by more than 40 percent.

The invention discloses a hericium erinaceus cultivation method comprising the steps of culture material preparation, fungi bag preparation, sterilization, inoculation, greenhouse construction, mycelium culturing, fruiting management, harvesting, and post management. The cultivation process provided by the invention is simple, and the cost is low. The cultivated hericium erinaceus has the advantages of fast mycelium germination, robust growth, uniform and neat primordium, ordered fruiting body growth condition, robust fruiting body growth, and high biological efficiency.

A Japanese Grifola frondosa cultivating method specifically comprises steps as follows: selecting wood chips of broad-leaf species as a main cultivation material, and selecting wheat bran and Indian meal as nutrition sources; and then performing material mixing, bagging sterilization, inoculation, cultivation management, fruiting management and harvesting according to routine methods. During the cultivation management process, the temperature in a cultivate chamber is maintained to be 22-23 DEG C, the humidity is 60%-70%, and when white hypha lumps are formed on the surface of a fungus bag, gray primordium are formed and transparent water droplets are formed on the surface, 50 Lux lighting stimulation is required; and in a fruiting management process, when all the primordiums change into black and the water droplets are reduced, a ventilation silicone window at the upper part of the bag is required to be cut open by a cutter blade promptly, meanwhile, the temperature of the fruiting chamber is maintained to be 16-18 DEG C, the humidity is above 95%, and the lighting condition is maintained to be 500 Lux. With the implementation of the Japanese Grifola frondosa cultivating method, the condition that the precedent that industrial cultivation of the Grifola frondosa inland is not started yet at present is broken through, further, the production efficiency is quite high, and the market benefits are quite good.

A granular nutritive additive for culturing edible fungus is composed of core containing crude protein and the water-insoluble coated layer which can be decomposed into carbonhydrate by the mycelia of edible fungus. It can be added to the culture medium of edible fungus to greatly increase the yield of edible fungus.

The invention belongs to the technical field of multifunctional foliage fertilizer and provides a method for preparing chelating magnesium. The preparation steps are as follows: preparing 20% of magnesium carbonate suspension first by volume percent, and adding the 20% of magnesium carbonate suspension during the preparation process of compound microbial activated solution; and the specific method comprises the steps of combining the following materials by volume ratio: 3 % to 5% of EM stock solution, 2% to 5% of honey, 25% to 30% of the magnesium carbonate suspension and 60% to 70% of deionized water, blending the mixture uniformly, and putting the mixture in a rotary shaking table with the speed of 120 r / min to 180 r / min and fermenting for 6 to 7 days under ambient temperature. Therefore, chelating magnesium can be obtained. The method has the advantages that the magnesium fermented with compound microbe is in a chelating (complexation) state or an anion cation balance state, is easily insoluble in water, and is easy to be absorbed by plants; and when the chelating magnesium is applied on leaves, the chlorophyl content of vegetables can be improved, and the problem of physiological barrier of plants caused by lack of magnesium can be solved effectively.

The invention discloses a method for transforming 1,3-propylene glycol into 3-hydroxypropionic acid by applying resting cells, and belongs to the field of biological engineering. Through the method, firstly a rapid screening method is utilized to screen a microbial strain which has high transformation performance and high production capacity and is identified as acetobacter sp. JDB-71, and the resting cells of the strain are utilized for high-efficiency catalysis of 1,3-propylene glycol into 3-hydroxypropionic acid in an aqueous phase system. The obtained acetobacter sp. culture process is simple, the transformation efficiency is high, and the product yield is large; transformation solution components are simple, so as to be benefitial for separation and purification of the product; and the thalli concentration can be artificially adjusted to shorten the production time during the transformation process, and thus a lot of energy and production costs are saved.

The invention relates to the application of 3, 4dihydro 8 hydroxyl 1 benzopyrane 1 ramification extracted from Beijing mutation metabolite of Xenorhabdus nematophila pathogenic bacillus to prohibiting plant pathogenic fungi, dissolving the benzopyrane ramification freeze dried pure products in axenic water, mixing into a mother solution in 1000 mug / ml, then separately using PDA culture medium to dilute the mother solution into solutions in 0.20 mug / ml-20 mug / ml. The benzopyrane ramification extracting technique is simple and the produced bacteria are easy to culture in batches; the benzopyrane ramification has stronger bacteriostatic activity to various plant pathogenic fungi, especially to plant Phytophthora palmi; and this compound is firstly used to prohibit plant pathogenic fungi, and its bacteriostatic activity is strong, and it is convenient and safe to use.

The invention discloses a base bait formula for mytilus crassitesta planktonic larva. The base bait formula comprises the following components according to the parts of a water body containing monodus subterraneus of same cell density: 65-72 parts of water body containing isochrysis galbana parke, 8-16 parts of water body containing nannochloris oculata or chlorella or a mixture of the nannochloris oculata and the chlorella, and 16-24 parts of water body containing diatom. The base bait formula has the advantages of complete nutrition, good luring property, low bait coefficient, low cost and convenience in culture.

The invention discloses a base bait formula for mytilus crassitesta planktonic larva. The base bait formula comprises the following components according to the parts of a water body containing monodus subterraneus of same cell density: 65-72 parts of water body containing isochrysis galbana parke, 8-16 parts of water body containing nannochloris oculata or chlorella or a mixture of the nannochloris oculata and the chlorella, and 16-24 parts of water body containing diatom. The base bait formula has the advantages of complete nutrition, good luring property, low bait coefficient, low cost and convenience in culture.

A process for puffing the culture medium of edible fungus, which is prepared from the twigs or the straw or stalk of agricultural crops includes such steps as pulverizing, proportionally mixing it with organic and inorganic auxiliaries, puffing and baking.

The invention relates to a pholiota nameko culture method, which comprises the steps of: culture material proportioning and preparation, sterilization, inoculation and bagging, mycelium culture, summer-crossing management and primordium inducement fruiting. The pholiota nameko culture materials comprise the following recipe and mixture ratios: 30 to 50 parts of corncobs, 30 to 50 parts of soft mixed hardwood sawdust, 10 to 20 parts of bran, 2 to 4 parts of corn starch, 0.5 to 1.5 parts of gypsum and 0.5 to 1.5 parts of lime. The culture raw materials adopt the corncobs and the soft mixed hardwood sawdust as major materials, a good basis is laid for the smooth implementation of the Natural Forest Protection Projects and the pholiota nameko planting in flat areas, planting trays are omitted, the culture cost can be saved by 15 percent, the culture process is simple and is easy to implement, the work efficiency is improved, the edge fruiting effect of the pholiota nameko bag type culture is improved by 30 percent through being compared with that of the disc type culture, the edge fruiting effect of the edible fungi is sufficiently utilized, the yield can be improved by more than 10 percent, and the high-quality fruiting rate is improved by more than 40 percent.

The invention discloses a method for producing DHA and astaxanthin by mixed culturing Schizochytrium and Haematococcus pluvialls, inoculating Schizochytrium in the fermentation liquid after the growth period of Haematococcus pluvialls to carry out mixed bacterial fermentation culture , Supplement carbon source and sodium glutamate during the fermentation process, and extract oil after the cultivation is completed. The invention has the characteristics of simplifying the cultivation process, reducing the production cost, improving the stability of oil extraction and the like.

The present invention relates to a process for producing flavone through liquid-solid double-phase culture of medicinal mushroom phellinus igniarius, and belongs to the technical field of biological fermentation engineering. According to the present invention, mainly during a phellinus igniarius liquid fermentation process, a macroporous absorption resin is added to a culture liquid to establish a liquid-solid double-phase culture system so as to improve a flavone yield; with the double-phase culture process, the flavone yield can be up to 2174.35 mug / ml and is increased by 35% compared with the control; and the process has characteristics of simple operation and strong generalization, and is especially suitable for large-scale higher fungi fermentation and active substance production.

The invention discloses a stropharia rugoso-annulata culture medium and a preparation method and application thereof.According to the stropharia rugoso-annulata culture medium, perishable organic waste is adopted as raw materials and comprises garden waste and pre-meal waste, and the garden waste comprises but not limited to branches and fallen leaves; the pre-meal garbage comprises but is not limited to fruits, vegetables, food leftovers and melon and fruit peels discarded in family daily life. According to the method, the perishable organic waste is used for manufacturing the culture medium to replace a traditional stropharia rugoso-annulata culture medium raw material, the idea of resource cyclic utilization is exerted and practiced to the maximum extent, the resource utilization level of the perishable organic waste is greatly improved, and the new mode that non-woven bags are used for indoor culture is achieved; therefore, a traditional stropharia rugoso-annulata cultivation mode is broken, the cultivation technology is simplified, and the cultivation process is optimized and simplified.

The invention provides a method for growing dendrobium seedlings, which includes in vitro inoculation of seeds, formation of circular corms through cultivation, and formation of differentiation seedlings by the cultivation of the circular corms, and is characterized by steps as follows: inoculating the differentiation seedlings on a 1 / 2 MS culture medium in a seedling raising bottle; covering with a lid; sealing the part of the lid which is connected with the bottle body with a fresh-keeping film; then placing the bottle in an ordinary greenhouse with a shading rate of 60-80% and after growing in a natural lighting environment with a temperature of 5-34 DEG and illumination intensity of 3500-9000Lx for 2 months, taking the seedlings out of the bottle and permanent-planting in a field. Theinvention can save not only the electric energy consumed in the commodity seedling culturing phase, but also the cost incurred by sterilization, sterilization medicine and manpower, and is convenientto be operated, time-saving and labor-saving. Particularly, after the culturing in the natural lighting environment, the antibacterial and disease-resistant capability of the commodity seedlings is improved, and the planting survival rate of dendrobium seedlings is greatly increased and attains over 90%.