145 results about "Transfer cell" patented technology

The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.

A system, device and method for electroporation of living cells and the introduction of selected molecules into the cells utilizes a fluidic system where living cells and biologically active molecules flow through a channel that exposes them to electric fields, causing the molecules to be transferred across the cell membrane. The device is structured in a manner that allows precise control of the cells location, motion, and exposure to electric fields within the flow channel device. The method is particularly well suited for the introduction of DNA, RNA, drug compounds, and other biologically active molecules into living cells.

In a broadband switching system for the switching of asynchronously transferred cells of data, a dynamic bandwidth controller (DBC) controls the application of data cells to an input port of the system. Data cells are supplied by a number of transmitting end-systems. When an end-system begins transmitting data cells, the DBC detects the presence of incoming cells and requests bandwidth from a connection admission control (CAC) forming part of the system. The switching system stores a table associating a number of signal sources connected to the ingress with respective predetermined transmission bandwidths and, preferably also, maximum delay times. When arrival of cells from one of the sources at the input port is detected, the DBC sends a request signal for the relevant predetermined bandwidth to the CAC and delays transmission of the cells until at least the predetermined bandwidth is allocated. This delay is typically effected by sending a cell rate indicator signal back to the input port for placing the source in a halt mode. If no allocation of bandwidth has occurred before the respective maximum delay time, bandwidth is allocated by robbing bandwidth form other signal sources.

The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrodes or hollow fiber electrodes, are provided, (b) the microelectrodes are connected to a power supply, (c) the electrodes, individually controlled by high-graduation micromanipulators, are placed close to the cell structure at an appropriate inter-electrode distance, and (d) a highly focused electric field of a strength sufficient to obtain electroporation is applied between the electrodes. The method may be used in order to transfer cell impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure, in biosensors, in the treatment of tumours and neurodegenerative diseases and in the study of biophysical processes.

A microscope slide assembly is provided. The microscope slide assembly comprises a slide and a mask with a window that defines an examination area on the slide. Preferably, the mask further comprises a tab to facilitate removal and markings to indicate its presence. Optionally, the mask further comprises a plurality of windows that define a plurality of respective examination areas on the slide. A method for transferring cells onto a microscope slide is also provided. The method comprises transferring cells to a slide with a mask disposed thereon, removing the mask to remove unwanted cells, and recovering the removed cells from the mask.

The entire process of reverse transcription-polymerase chain reaction (RT-PCR) is simplified by using oligonucleotide-immobilized microplates made of, e.g., polypropylene, to which oligonucleotides are securely immobilized and which can be subjected to thermal cycles of PCR. RT-PCR is preferably conducted in solid-phase. Capturing of mRNA and RT-PCR can be conducted in the same plates. The cDNA synthesized from the mRNA captured on the microplates can be used more than once. Further, in combination with the microplates, a filter plate is used for the preparation of cell lysates wherein target cells are placed on the filter plate, and a lysis buffer is passed through the cell layer on the filter to transfer cell lysate directly to the microplate via well-to-well communication.

The present invention relates to a method for culturing cells, which comprises the steps of: causing cells to adhere to the surface of a cell array substrate having a cell adhesiveness variation pattern that comprises regions having good cell adhesiveness and regions having inhibited cell adhesiveness patterned on a substrate; transferring the adhered cells to a cell culture substrate in such patterned state; and culturing the transferred cells.

Disclosed is a method for parallel delivery of agents to and / or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.

By maintaining the cell density during culture at an appropriate level and eliminating the procedure of transferring cells from a container to another container when a large amount of cells are cultured, damage on cells can be reduced, risk of contamination can be lowered, labor saving can be attained and automation can be attained.A cell culture apparatus including: a culture container 11 formed of a soft packing material and having an enclosure part 11-1 in which a culture medium and / or cells being cultured are enclosed is placed; a container table 12 on which the culture container 11 is placed; a roller 13 which is placed on the upper surface of the culture container 11 and divides the enclosure part 11-1 into two or more chambers; and a driving means 15 which relatively moves the roller 13 or the culture container 11, thereby to change the volume of the culture part 11-11 in the enclosure part 11-1 in which a culture medium or cells being cultured are enclosed.

The invention discloses an electronic purse air transfer cell phone intelligent card, a cell phone terminal, a system and a method. The offline electronic purse air transfer application of at least one capital source is written into the cell phone intelligent card; when the application which is selected by a user is acquired, the user is reminded to input the transfer amount and a transaction password; an application classification code is inquired, whether any unconfirmed transaction record exists is confirmed, and an uplink data short message is sent to an air transfer platform; the air transfer platform calls a corresponding application system through an external interface, the application system calibrates the transfer amount and the transaction password, and deducts money; and the cell phone intelligent card adds money to an electronic purse and confirms that the transfer succeeds. According to the invention, not only is the access of a plurality of capital resources supported, but also the system can be conveniently extended and upgraded.

The invention discloses a mobility load balancing method based on an AHP (Analytical Hierarchy Process) in an LTE system. The method mainly comprises the steps of selection of an optimal load transfer cell and determination of optimal CIO (Cell Independent Offset) parameters. According to the method, four modules namely a cell state detection module (1), a user-cell matching pair generation module (2), a load distribution module (3) and a balancing module (4) are adopted, wherein the cell state detection module is mainly used for judging a cell load state and initiating load balancing if a cell is overloaded; the user-cell matching pair generation module is mainly used for selecting an optimal transfer cell of a user through an AHP algorithm; the load distribution module is mainly used for pre-distributing the load quantities of the overloaded part of the overloaded cell to each target cell before the CIO parameters are adjusted; the load of each target cell is smaller, the distributed load quantities are larger; the balancing module is mainly used for adjusting the CIO through a variable step; when each target cell completely receives the distributed load quantities, the optimal CIO of the cell is obtained.

The present invention provides methods and devices for screening a single cell or a small group of cells for a desired biological activity. In particular, the present invention provides for delivering cell(s) to a plurality of cell isolation regions of a cell isolation device, transferring cell(s) to a plurality of wells of a cell expansion device and then detecting the potential desired biological activity of the cell(s). Each of the receptacles comprise a recess sized to isolate a single cell or small group of cells and each of the wells encompass a cavity that provides sufficient volume for cell proliferation.

The present invention relates material that is useful in culturing and transferring cells as well as delivering cells. The material comprises plant derived cellulose nanofibers or derivatives thereof, wherein the cellulose nanofibers are in a form of a hydrogel or membrane. The invention also provides methods for producing these materials and compositions and uses thereof.

The invention discloses a load balancing method among nonadjacent heterogeneous cells in a ubiquitous network, and mainly resolves the problem that in the prior art, when load of an adjacent cell is too heavy and load to be transferred of a current cell cannot be received, load balancing cannot be achieved. The load balancing method includes that load information of the current cell, the adjacent cell and a double bounce cell is collected periodically through wireless access points (AP), and whether load balancing is to be achieved or not is judged according to whether uniformization load of the current cell exceeds a load balancing starting threshold or not; according to the load information of the adjacent cell and the double bounce cell, whether load is transferred to the adjacent cell or to the double bounce cell is judged; a switching target is chosen, and according to switching cost of the switching target, the optimum load transferring cell with the minimum switching cost is chosen; and the switching target is indicated to be switched into a corresponding cell to complete the load balancing. According to the load balancing method, the load balancing among nonadjacent heterogeneous cells is achieved, the range of the load balancing is enlarged, network performance and the resource utilization rate are improved, and the load balancing method can be used for resource optimization in a heterogeneous communication environment.

A device for transferring cells present in fluid media is provided which is especially useful for in vitro fertilization techniques and intracytoplasmic sperm injection procedures.

The invention discloses a terahertz flow cytometry sensor for label-free detection of single or small quantity of living cells and a detection method of the terahertz flow cytometry sensor. The sensor comprises a cell microdroplet preparation module, a microfluid detection passage and a THz detection module. The cell microdroplet preparation module comprises at least one microfluid tube cavity which comprises an inlet passage a and two inlet passages b, the inlet passage a is used for transferring cell suspension, the two inlet passages b are used for transferring an OCA (optical clearing agent) and in symmetrical linkage with the inlet passage a, and an outlet passage d used for transferring cell microdroplets is connected at the joint of the three inlet passages. The THz detection module comprises at least one detection groove identical to the microfluid detection passage in size, and the outlet passage d is connected with the detection groove through the microfluid detection passage. By the terahertz flow cytometry sensor, the single or small quantity of living cells can be uniformly wrapped in the OCA to form independent 'cell microdroplets' sequentially passing a THz detection position, so that label-free detection of the single or small quantity of living cells is realized.

A method for repairing a retinal system of an eye, using bucky paper on which a plurality of retina pigment epithelial cells and / or iris pigment epithelial cells and / or stem cells is deposited, either randomly or in a selected cell pattern. The cell-covered bucky paper is positioned in a sub-retinal space to transfer cells to this space and thereby restore the retina to its normal functioning, where retinal damage or degeneration, such as macular degeneration, has occurred.

The invention relates to a load balancing method based on fog computing and a cooperative communication network. The method comprises the following steps: (a) obtaining an overloaded cell; (b) obtaining a transfer user in the overloaded cell; (c) obtaining a transferred cell of the transfer user; (d) obtaining a user who ultimately executes a task; (e) obtaining a task transfer mode of the transfer user; and (f) transferring the task of the transfer user to the user who ultimately executes the task for transmission and execution. In the load balancing method provided by the embodiment of the invention, a better load balancing method is provided by comprehensively considering the time delay and the energy consumption of the user, thereby improving the application experience of the user andthe system performance, and reducing the overall energy consumption and the time delay of the system.

The present invention provides one method of using methanol utilizing yeast, especially transferred recombinant saccharomycete with ectogenic S-adenosyl-menthionine (SAM) synthetase expression vector, to produce SAM and the method of constituting the expression vector and transferred cell. The recombinant saccharomycete is used in fermentation of culture medium with methanol, proper amount of methionine and certain nitrogen source, high yield of SAM may be obtain and SAM content in cell may reach 10-20 % of dry cell weight. The present invention also provides one methanol utilizing Pachia pastoris sacchromycete strain with preserving number of CGMCC No.0648.

The invention relates to a division measurement device with small section, comprising dividing cell, transferring cell for flux signal, signal-modulated and A / D conversion cell, and computer collection and processing cell. The flux in dividing pipe is converted to electrical signal by electromagnetic flowmeter and is connected with signal-modulated and A / D conversion cell. The signal-modulated and A / D conversion cell mainly comprises filtering, amplification and A / D conversion. Digital signal obtained is connected with computer collection and processing cell by anti-interference signal wire and by software the actual flux of dividing pipe is calculated so as to infer the returning flux of oil well. Non-contact Doppler measuring device comprises conversion cell group of flux signal, signal-modulated and A / D conversion cell, and computer collection and processing cell. As the empty flux of well ring at the position with different directions and layers is measured by conversion cell group of flux signal the returning flux of well with higher accuracy is obtained. The equipment measures the returning flux of well fluid in time and is provided with high measuring accuracy and little signal error.

To provide a culture container that can transfer cells cultured under a plurality of different culture conditions by a simple work. Provided is a multi-chamber culture container obtained by processing a material having gas permeability, wherein two or more culture chambers for culturing cells are formed, each of the culture chambers are intercommunicated with at least of one other culture chamber and at least one external connection part for intercommunicating with the outside of the multi-chamber culture container is provided.

The invention discloses the network transmission method for increasing the reliability for transferring cell as well as the communication realization system. The characters of the method and system are as follows. The method decomposes the virtual circuit number into the two parts of the link identification and the session identification explicity. Network seeks the route by using the link identification number only. The application of the terminal host computer and terminal uses the link identification number and the session identification to make the communication connection. The cell sequence number is set on the information head. The said number is increased by degrees in turn within a session connection and the session application sorts the reached cells based on the said cell sequence number. The transmission reliability can be further increased by the feedback retransmission.

Disclosed is a method for sequential delivery of agents to and / or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and / or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.

Devices, methods, and kits directed towards collecting and preparing cells using a separable sample collection layer may be configured to collect or treat cells from a liquid sample with mechanisms for easy transfer of the cells prior to analysis or imaging. The separable sample collection layer may comprise a porous membrane that cells may be collected on, and one or more support layers comprising tape with one or more adhesive coatings and release liner. The devices, methods and kits may be configured with support layers comprising cutouts that form vertically or horizontally oriented microchannels for efficiently removing undesirable liquid. Following collection and / or treatment, cells collected onto the porous membrane may be adhered to another surface for further processing or analysis.

The invention relates to a nerve stem cell injection made from retina pigment epithelial cell, to treat paralysis agitans, wherein said injection at least comprises 3*106 nerve stem cells of human hRPE, while said nerve stem cells are cultivated from retina pigment epithelial cells. And its production comprises that: (1), separating and cultivating cells, that separating the hRPE cells, mixing uniformly to prepare single cell suspension; (2), hRPE cell cultivation and generation that digests original cultivation liquid, ending digestion, blowing and beating to form cell suspension, eccentrically filtering and removing the upper clear liquid, adding cultivating substrate, to disperse and form cell suspension, filtering with cell screen, transferring cell into cultivating bottle, generating to at least fifth generation; (3), adding common salt into hRPE nerve stem cell, to prepare cell suspension injection.

This disclosure provides a method of isolating peptides having cell-penetrating function, wherein the peptides are detected as biotinylated molecules only following their translocation through the cell membrane. The disclosure also provides methods for validating the cell-penetrating function of the peptides, or that may be employed in their own right to isolate such peptides, wherein the peptides are detectable by virtue of their ability to transport a detectable cargo into the cytoplasm, such as a cargo toxin or a fragment of a green fluorescent protein (GFP) that is required for complementation of a functional GFP. The disclosure also provides non-canonical peptides having cell-penetrating function that differ structurally from known CPPs such as TAT, VP22, transportan and penetratin, and that are capable of translocating cell membranes and escaping the endosome. The disclosed peptides have utility in transporting cargo therapeutics and diagnostics into cells.

Example driver circuits can utilize shared-charge recycling charge pump structures. In particular, an example shared-charge recycling process may be applied to a clock buffer and charge transfer cells of the charge pump in a driver circuit. An example recycling process may include recycling of shared charges between the capacitors / capacitances in the charge transfer cells. An example recycling process may use the charges in one or more capacitors to charge one or more other capacitors before the charges are wasted or otherwise discharged to ground. Such recycling may significantly reduce the power consumption of the charge pump while still providing a high output voltage level, according to an example embodiment of the invention.

The invention discloses a pneumatic correction device for cell transferring. The pneumatic correction device for cell transferring comprises a base, a cylinder, a pair of combination baffles, a pair of U-shaped clamp arms, a proximity switch, and a sensing element. The base comprises a pair of side plates and a top plate located at the top of the side plates. The cylinder is disposed below the top plate. The combination baffles are symmetrically disposed on a guide bar on two sides of the cylinder respectively and located below the cylinder. The U-shaped clamp arms are symmetrically disposed on the combination baffles respectively. The sensing element is connected with an air hole of the cylinder. The proximity switch provides cylinder operation signals through the sensing element so as to allow the guide bar on the cylinder to contract or extend to drive the U-shaped clamp arms to move, thereby correcting the position of a cell. The pneumatic correction device is disposed on a travel arm used for transferring cells. The position of the cells on the travel arm can be corrected by the pneumatic correction device, and accordingly the problems of lower precision and proneness of cells to deflection and fragmentation in transfer process caused by mechanical wear of the travel arm in the prior art are solved.

The invention belongs to the technical field of cell engineering, and particularly relates to an efficient screening method of an exogenous protein expression cell strain. The efficient screening method of the exogenous protein expression cell strain disclosed by the invention comprises the following steps: using an interest protein expression vector to transfect host cells; pressurizing a screening drug, and forming a stable cell pool; inoculating a semisolid culture medium with the cells in the stable cell pool; transferring the cloned cells from the semisolid culture medium to a 96-hole cell plate to be continuously cultured for 4-5 days, and detecting the interest protein expression index in the cell supernatant; transferring cells which are first 50 in the ranking of expression index to a 24-hole plate to be continuously cultured for 5 days; further transferring the cells in the 24-hole plate to a 6-hole to be continuously cultured for 5 days; transferring the cells in the 6-hole plate to a shake flask for culture, and detecting and evaluating the interest protein expression indexes of different cloned cells under a suspension state; according to the evaluation result in the shake flask, performing a stability passage test on the cells which are first 10 in the ranking of the expression index; according to the interest protein expression level of the cell strain and the stability of the continued expression protein, determining the candidate cell strains.

The invention discloses an electrokinetic remediation enhancing device and a method using same to remove heavy metal in sludge. The electrokinetic remediation enhancing device comprises an electrokinetic remediation system and a pH (potential of hydrogen) control system, wherein the electrokinetic remediation system comprises an electrokinetic remediation electrolytic bath, a cathode electrolytic cell and an anode electrolytic cell; the cathode electrolytic cell and the anode electrolytic cell are positioned at two ends of the electrokinetic remediation electrolytic bath; an ion enhancing transfer cell and activated carbon are arranged in the electrokinetic remediation electrolytic bath. The method using the electrokinetic remediation enhancing device to remove the heavy metal in the sludge comprises the following steps of (1) sludge pretreatment: pretreating a wet sludge sample by 0.5 to 3mol / L of sulfuric acid, so as to obtain the sludge with the water content of 70 to 85%; (2) putting the pretreated sludge into the ion enhancing transfer cell of the electrokinetic remediation electrolytic bath layer by layer; (3) energizing for 3 to 10 days under the condition of potential gradient of 0.5 to 2V / cm, and controlling the pH (potential of hydrogen) value of a catholyte to 2 to 4. The electrokinetic remediation enhancing device and the method have the advantages that the problem of difficulty in transfer of metal ions in the sludge is solved, and the electrokinetic remediation rate of the heavy metal in the sludge is improved.