290 results about "Cell isolation" patented technology

The invention relates to a method for freezing and reviving umbilical cord tissues and for separating and increasing stem cells after reviving the umbilical cord, and the step comprises the following steps of preparing umbilical cord tissue freezing liquid; sterilizing and washing the umbilical cord tissues; cutting the tissues into blocks; placing the tissue blocks and the freezing liquid into afreezing tube, cold storing the tissue blocks for 0.5 hour under the temperature condition of 4 DEG C and then storing the tissue blocks for one day under the temperature condition of minus 80 DEG C,and then freezing the tissue blocks in liquefied nitrogen; and taking the umbilical cord tissues out of the liquefied nitrogen to use, thawing the umbilical cord tissues in constant-temperature waterbath, utilizing mesenchymal stem cell culture base to washing the umbilical cord tissues through a drop method, and separating and increasing the mesenchymal stem cells of the revived umbilical cord tissues through a tissue attachment method. The method can effectively protect the frozen umbilical cord tissue, so that the umbilical cord tissues can be revived to use, and the method is particularly suitable for separating and increasing the mesenchymal stem cells after the umbilical cord tissue is revived.

The present application describes various applications of the non-expansion protocol for the preparation of an injectable autologous cell mixture of the present invention that can be used to prevent symptoms in a number of indications. Cells are isolated from surgically derived tissue and are at least partially disaggregated from each other. The heterologous cell mixture is mixed with growth factors, differentiation agents, extracellular matrix proteins and / or microspheres and injected into the patient without cell expansion. The harvesting of tissue, cell isolation, and injection are performed within a single surgical procedure lasting only minutes to hours.

This invention provides: agents determined to be capable of specifically inhibiting the fusion of a macrophage-tropic primary isolate of HIV-1 to a CD4+ cell, but not a T cell-tropic isolate of HIV-1 to a CD4+ cell; and agents determined to be capable of specifically inhibiting the fusion of a T cell-tropic isolate of HIV-1 to a CD4+ cell, but not a macrophage-tropic primary isolate of HIV-1 to a CD4+ cell. This invention also provides: agents capable of specifically inhibiting the fusion of a macrophage tropic primary isolate of HIV-1 with a CD+ cell susceptible to infection by a macrophage-tropic primary isolate of HIV-1; and agents capable of specifically inhibiting the fusion of a T cell-tropic isolate of HIV-1 with a CD4+ cell susceptible to infection by a T cell-tropic isolate of HIV-1. The agents include but are not limited to antibodies. This invention further provides: methods of inhibiting fusion of a macrophage-tropic primary isolate of HIV-1 with a CD+ cell susceptible to infection by a macrophage-tropic primary isolate of HIV-1 which comprises contacting the CD4+ cell with an amount of an agent capable of specifically inhibiting such fusion so as to thereby inhibit such fusion; and methods of inhibiting fusion of a T cell-tropic isolate of HIV-1 with a CD4+ cell susceptible to infection by a T cell-tropic isolate of HIV-1 which comprises contacting the CD4+ cell with an amount of an agent capable of specifically inhibiting such fusion so as to thereby inhibit such fusion.

A particle / cell separation device is described which is particularly adapted for neutrophil depletion from a preparation of whole blood or platelet-rich plasma. Also described are blood and platelet rich plasma compositions produced using the device which are neutrophil-depleted.

A one-step real-time system for treating, reconstructing or augmenting a breast tissue defect in a patient comprising means for extracting adipose tissue, isolate adipocytes, process and re-inject stem and regenerative cells to the breast of the patient.

The invention relates to an acquisition method of adipose-derived stem cells. The method comprises the following specific steps of: digesting adipose tissues by use of mixed collagenase; culturing adipose-derived stem cells obtained through digestion in a culture solution; carrying out solution replacement and transfer of culture; and detecting to obtain the qualified adipose-derived stem cells. According to the invention, the problems that in the traditional cell separation method, cells can not be thoroughly digested by collagenase and the cell purity is low can be solved; and a separation method of adipose-derived stem cells is improved and optimized, so that tissues can be digested more thoroughly, and the purity during a culture process is higher, thus a more excellent seed cell source is ensured.

The invention relates to methods for separating, freezing and thawing a whole cell of an umbilical cord and separating and augmenting a thawed stem cell. The method for separating and freezing the whole cell of the umbilical cord comprises the following steps of: sterilizing and cleaning an umbilical cord tissue; shearing the tissue into a block shape and carrying out digestion treatment for 1.5 hours; preparing a umbilical cord tissue frozen solution for later use; and adding the whole cell and frozen solution which are obtained by sterilizing treatment into a freezing tube, refrigerating the freezing tube for 0.5 hour at low temperature of 4DEG C, freezing the freezing tube for one day under the temperature condition of -80DEG C and then freezing the freezing tube in liquid nitrogen for later use. The method for thawing the whole cell of the umbilical cord comprises the following steps of: when the whole cell of the umbilical cord is required, extracting the whole cell of the umbilical cord from the liquid nitrogen, thawing the whole cell of the umbilical cord in a constant-temperature water bath; cleaning the whole cell of the umbilical cord by using a mesenchymal stem cell culture medium and a drop method; and augmenting a mesenchymal stem cell by using the thawed whole cell of the umbilical cord through cell culture and cell passage. According to the methods disclosed by the invention, the frozen umbilical cord tissue can be effectively protected and is convenient for thawing; and the method is especially suitable for separating and augmenting the mesenchymal stem cell after the frozen umbilical cord tissue is thawed.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.

The present invention provides a rare cell isolation device including: a first body which is disposed above a filtration membrane and includes a first inlet for injecting a biospecimen; and a second body which is disposed under the first body and bonded to the filtration membrane, wherein the first body and the second body have a disk-shaped structure to be rotatable around their centers, and the filtration membrane is disposed to be separated from the center of the second body in a radial direction.

The invention provides a separating method and an application of fat stem cells. The method provided by the invention comprises steps that: (1) an obtained fat cell tissue material containing fat stem cells is washed by using a washing liquid; (2) the material is digested by using collagenase, such that a digested tissue mixture is obtained; (3) the tissue mixture is filtered, such that a filtrate containing the fat stem cells is obtained; and (4) the filtrate is centrifuged, and an obtained precipitate is a fat stem cell mass. With the method provided by the invention, fat stem cells can be effectively separated; the purity of the obtained fat stem cells is high; and the proliferation speed of the fat stem cells is high. The fat stem cells separated by using the method provided by the invention can be applied in regeneration medicine, anti-aging treatments, and the like.

It is an object to achieve a closed system that has a simple structure without resorting to large scale equipment and that sequentially carries out all of the step of separating useful cells, the step of culturing the useful cells separated in the separation step, and the step of washing and concentrating the cells cultured in the culturing step and to enable the preparation of useful cells having high safety and quality, while improving operability. A disposable set for cell culture includes a cell culture container (CC) having a liquid inlet (Lin) and a liquid outlet (Lout), a cell separation kit (A) that is for separating cells used for cell culture and is connected to the liquid inlet (Lin), and a cell collection kit (B) that is for washing and concentrating cultured cells in the cell culture container (CC) and is connected to the liquid outlet (Lout), and a cell culture device uses the disposable set for cell culture and includes flow path on-off valves and pumps provided at appropriate positions of pipelines of the disposable set and a controller controlling them.

Disclosed is a method for detaching cultured cells that can selectively detach cultured adhered cells. The scaffold to which the cells adhere is configured from at least a cell adhesion factor containing carbon nanotubes, and by means of radiating laser light in a spot shape on the scaffold in a region where cells are adhered, a shock wave is generated by the heat arising by means of the photothermal conversion of the carbon nanotubes, and by means of this shock wave, the cells are caused to be in a non-adhered state.

Present invention relates to magnetic fluids for biologic medicine. It contain water, magnetic nano granule and hydrophilicity organic high polymer substance, wherein hydrophilicity organic high polymer substance covered on magnetic nano granulometric surface, hydrophilicity organic high polymer magnetic nano granule homodisperse in water phase, magnetic nano granule being magnetic inorganic nano granule, hydrophilicity organic high polymer substance being water-solubility whose coating layer surface having functional group hydroxy-OH or aldehyde group-CHO, magnetic inorganic nano granule being particle diameter of 5-20 nm of Fe<3>0<4>, the particle diameter of magnetic inorganic nano granule compound granule covered with water-solubility starch bring less than 50nm.Said magnetic fluids has good biologic consistency, biodegradability, suspension stability and magnetic responsibility, can be used in cell separation, tumour heat treatment, nuclear magnetic resonance radiography imaging and target drug delivery etc biomedical field. Said invention provides two magnetic fluids preparation methods.

The invention relates to a centralized prison passageway access control system, which comprises a centralized access control system arranged on a prison gate and used for access to the prison gate, an access control system arranged on the door of a cell isolation area and used for access to the cell isolation area, an access control system arranged on the door of a cell building or floor and used for access to the cell building or floor, access control systems arranged on the doors of cells and used for access to the cells, an access control system arranged on the door of a prison office area and used for access to the prison office area, and an integrated information security platform. By using the centralized prison passageway access control system, the implementation of the daily supervision work of a functional department is more benefited, the ability of a prison in dealing with emergencies is further enhanced, and the control and management of incoming and outgoing people and all gates and doors by the prison are greatly enhanced.

The present invention pertains to a system for culturing cells comprising a culturing bag and a continuous flow centrifuge wherein the cells are continuously separated from the supernatant and are recycled into the culturing bag. Further provided are methods for culturing cells and for producing a biological substance using the device for culturing cells, and the use of a bag for culturing cells in said device or said methods. In particular, a perfusion system for culturing cells is provided wherein the wave technology for culturing cells is combined with continuous flow centrifugation for separating the medium from the cells.

The invention discloses a tissue-engineered bone cartilage composite scaffold and a preparation method thereof, belonging to the technical field of biomaterials. The scaffold is of a multilayer integrated structure and comprises a cartilage tissue scaffold layer, a cartilage tissue calcified layer, a porous cell isolation membrane and a bone tissue scaffold layer. The cartilage tissue scaffold layer is inoculated with cartilage cells, and growth factors for promoting formation of cartilage are introduced to promote growth of cartilage cells. Main raw materials like heparan sulfate proteoglycan with good biocompatibility and degradability are selected and used and the method consisting of a cross-linking reaction, freeze drying, directional pore formation, compounding of multiple layers, overall integration and the like are carried out so as to obtain the functionalized multilayer integrated tissue-engineered bone cartilage composite scaffold with good mechanical properties. According to the invention, micro-nano hydroxyapatite crystal, degradable non-stoichiometric polyethylene glycol / poly(epsilon-caprolactone) nanometer coaxial short fiber, RGD-grafted heparan sulfate proteoglycan / oxidized sodium alginate and N-succinyl chitosan are compounded together to prepare the composite scaffold; and the composite scaffold is mainly used for restoration of full-thickness defects of articular cartilage and subchondral bone.

A method for forming a memory cell and periphery circuit includes providing a substrate with a peripheral circuit region and a memory cell region. A mask layer is formed on the substrate to define multiple active regions in the peripheral circuit region and to define multiple channel regions in the memory cell region. Multiple field oxide layers are formed between the active areas, and Dopants are implanted in the substrate between the channel regions. Multiple inter-cell isolation layers are formed between the channel regions and the dopants are driven in the substrate to form buried diffusion regions. The mask layer is removed. A layer of electricity-storage material and multiple word lines are formed on the substrate in the memory cell region.

The present invention relates generally to a method for the generation of a substantially homogenous population of undifferentiated cells. More particularly, the present invention relates to a method for isolating a substantially homogenous population of stem cells, and in particular, mammary stem cells (MaSCs). The MaSCs of the present invention are isolated on the basis of differential levels of proteins present on their cell surface. The MaSCs of the present invention are particularly useful as targets for identifying agents which modulate MaSC survival, self-renewal, proliferation and / or differentiation in both normal and diseased tissue such as, but not limited to, tumor tissue, and, also as source of tissue for the regeneration, replacement and / or augmentation of tissue damaged and / or lost after disease or injury.

The invention provides a separation method for mouse female germline stem cells in the technical field of transgenosis engineering. The separation method comprises the following steps: step one, collecting a mouse ovary, and adopting a two-step enzymic digestion method for preparing germline stem cell suspension; and step two, adding mvh first antibody and then second antibody magnetic bead in the cell suspension and re-suspending the cells, thus separating the female germline stem cells. The invention also provides a culture in vitro method for the mouse female germline stem cells, which comprises the following steps: transferring the female germline cells on an STO cell culturing layer, adding female germline stem cell culture solution, and conducting primary culture and subculture on the female germline stem cells, thus obtaining purified and stable germline stem cells. In the method, the mouse female fermline stem cells are separated and cultured in vitro successfully at first time, the methods are simple, the cost is low, the requirements on experiment conditions are low, and the operability is strong.

The invention relates to the technical field of adipose-derived stem cell isolated culture, and discloses a dental pulp stem cell isolated culture method. The dental pulp stem cell isolated culture method comprises the following steps: washing adipose tissues, digesting the adipose tissues by using collagenase Type I, separating adipose stromal vascular fractions, then carrying out scavenging-precipitation, planting and culturing obtained primary cells to obtain the primary cells in the end, and carrying out primary cell passage. The dental pulp stem cell isolated culture method has the beneficial effects that by standardizing the dental pulp stem cell isolated culture method and various parameters in concrete operations, the adipose-derived stem cell isolated culture efficiency is improved, the output rate is increased, and the production cost is reduced.

The invention provides a preparation method of a targeted superparamagnetic nano-probe. The method includes the steps of: 1. preparing ferroferric oxide nano-particles with a particle size of 10-20nm in an oil phase by high temperature thermal decomposition; 2. synthesizing an amphiphilic polymer; 3. conducting aqueous phase transformation of the ferroferric oxide nano-particles obtained by step 1 through the amphiphilic polymer obtained by step 2 so as to obtain water-soluble superparamagnetic nano-particles; and 4. under the coupling effect of EDC / NHS, subjecting the water-soluble superparamagnetic nano-particles obtained by step 3 to covalent coupling with a target molecule, thus obtaining the targeted superparamagnetic nano-probe. The invention also provides an application method of the probe. Under the action of a separation column and an applied magnetic field, the probe can specifically and effectively capture and separate target cells. Compared with the prior art, the probe prepared by the method provided by the invention has the advantages of uniform particle size, high T2 relaxation rate, good stability, high pH value, high salt tolerance, and good target cell separation effect.

The invention discloses a method for separating fetal nucleated red blood cells in maternal peripheral blood. The method comprises the following steps: (a) enriching nucleated cells, namely, (1) collecting whole blood, (2) centrifuging, and collecting cell precipitates, (3) preparing cell suspension, namely adding a cell culture medium to the cell precipitates, and uniformly mixing so as to prepare the dilute cell suspension, (4) putting a cell separating medium in a centrifugal tube in advance, and adding the dilute cell suspension to a layering liquid surface, (5) centrifuging so as to obtain a cell layer, and (6) sucking up the cell layer, adding Hank, s liquid, uniformly mixing, centrifuging at a room temperature, and washing cells, and (7) resuspending the cells; and (b) screening the fetal nucleated cells in the maternal blood, to be specific, (9) separating out the fetal nucleated red cells, and (10) carrying out quadruple morphology identification on nucleated red cell pictures obtained by sorting, and collecting the nucleated red cells meeting the standards for extracting the fetal whole genome DNA (deoxyribonucleic acid). According to the method, the purity and the recovery rate of the fetal nucleated red blood cells obtained by the separation and purification of the maternal peripheral blood are high.

The invention provides a kit for detecting human regulatory T cell subtypes and a detection method and belongs to the technical field of cell subtype detection. The provided kit comprises components as follows: a blood diluent, a mononuclear cell separation medium, a cell culture fluid, a lymphocyte activation solution, dead cell removal dyes, an FcR blocking agent, a fluorescence labeled antibodyresisting human cell surface labeling molecules, a fluorescence labeled antibody resisting human intracellular molecules, a PBS buffer solution, lipopolysaccharide, a washing buffer solution, a celldyeing buffer solution, a cell fixing solution and a permeable membrane lotion. During detection, firstly, mononuclear cells in whole blood are separated, then, mononuclear cells of human peripheral blood are stimulated, finally, a fluorescent antibody is stained, and the regulatory T cell subtypes can be detected. By use of the kit and the detection method, 2-6 regulatory T cell subtypes can be simply and rapidly distinguished.