70 results about "New Zealand Rabbit" patented technology

The invention relates to a colloidal gold immunochromatographic test strip for detecting shrimp allergen and a preparation method thereof, belonging to the technical field of immunoassay. The invention discloses the assembled test strip which takes a combination of shrimp allergen-tropomyosin cluster specific antibody and color-developing substance colloidal gold as a detection reagent, the test strip is used for detecting the shrimp allergen in a food, and the test strip has low cost, fastness and portability. The applied antibody is an extracted and purified polyclonal antibody from a New Zealand rabbit with tropomyosin immune health. Due to the adoption of the polyclonal antibody, the test strip has low cost, good stability and good repeatability, and can be used in the detection of different samples.

The invention relates to a measuring method of micro-capsule alga toxin in water. The method includes the steps as follows: firstly, compounding and appraising of the micro-capsule alga toxin; secondly, preparing and purifying of polyclonal antibody antibody: immunizing the New-Zealand rabbit by making the micro-capsule alga toxin holoantigen MC-LR-KLH as the immunity resource, and preparing the polyclonal antibody and purifying by ammonium sulphate according to the normal method; thirdly, preparing immune bead: coupling the micro-capsule alga toxin holoantigen MC-LR-BSA and the nanometer bead, and preparing the immune bead containing MC-LR-BSA; fourthly, embedding the antibody to the pyroxylin film; fifthly, producing testing board, combing the coupling mat of the magnetic scale MC-LR-BSA, the pyroxylin film embedding the polyclonal antibody, the sample mat, the sopping mat, the covering film, the testing board out card into a testing board; sixthly, sample testing, respectively adding standard goods and testing samples with different concentrations into the sampling holds on the testing board, the samples flow on the test paper through the chromatography effect, after 3 to 5 minutes of the reaction under the room temperature, the testing board is put into a magnetic single testing apparatus to be tested, and the testing apparatus can further output the biology reaction signals being transferred to the magnetic field signals by the way of the electric signals; after the standard curve being drawn, the specific value of the micro-capsule alga toxin content in the sample to be tested is counted based on the standard curve.

This invention supplies a pure oral and maxillofacial squamous-cell carcinoma cell system of the New Zealand rabbit and its construction method or application. The property of the talked maxillofacial squamous-cell carcinoma cell system is stable, and it can stably multiply many generations. It is suitable to construct model of the maxillofacial squamous-cell carcinoma cell system animal, and it is the ideal cell system of base and clinic early application of the maxillofacial squamous-cell carcinoma cell.

A method for setting up animal model of vascular erection dysfunction includes using New Zealand rabbit to add effective amount of cholesterol and lard in its feedstuff, feeding this for certain period then executing dilatation plasty of internal iliac artery at two sides of saccule catheters to damage its internal skin of internal iliac artery, carrying out detection and identification by mating test, dicaryon or injecting blood active medicine on cavernous body after operation.

The invention discloses a swine GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase) protein antibody and a preparation method and application thereof. According to the swine GAPDH protein antibody and the preparation method thereof, an immunogen is prepared through expressing and purifying swine GAPDH protein, and a swine GAPDH protein resisting rabbit antibody is prepared through immunizing purebred New Zealand rabbits, so that the gap that swine specific antibodies are lacking is made up, and materials are provided for the scientific research on the genetics and breeding of swine; compared with the human GAPDH resisting antibodies purchased on the market, which can distinguish mouse GAPDH proteins only in a limited manner and can hardly distinguish swine GAPDH proteins, the swine GAPDH protein antibody prepared by the method has the advantage that GAPDH proteins of the swine and mice can be distinguished excellently.

The invention discloses a method for preparing desensitizing konjac flour for cosmetics. The preparation method integrates technologies of superfine grinding, bio-enzymatic treatment, ultrasonic wave and microwave treatment. On the premise of guaranteeing excellent water absorption, moisture retention, biocompatibility, film forming property and other performances of the konjac flour, animal skin tests prove the safety of the konjac flour in cosmetics. According to the method, the sensitizing ingredient in the konjac flour can be eliminated, and the content of gulcomannan in the konjac flour is increased (over 93 percent), so that the application of the konjac flour is expanded to the fields of cosmetics and medicinal appliances, and the additional value of the konjac flour can be increased. The konjac flour prepared by the preparation method is tested in acute dermal toxicity test, skin irritation / corrosion test, multiple skin irritation tests, skin sensitization tests, acute ocular irritation and the like on New Zealand rabbits and guinea pigs which are tested animals according to Hygienic Standard for Cosmetics (2007 edition) of Ministry of Health P.R. China, and the safety of the konjac flour is proven.

The invention relates to a matrix protein monoclonal antibody for appraising a mineralized stimulating factor and an appraising method. The monoclonal antibody specifically distinguishes the matrix protein of Pinctada martensi. The appraising method comprises the following steps of: culturing pallium cells of the Pinctada martensi to obtain a culture medium of primary culture; respectively adding stimulating factors required to be appraised in the culture medium, and then culturing to obtain a cell culture medium; adopting a hybridoma method to obtain a mice monoclonal antibody; using the matrix protein to immunize a New Zealand rabbit to obtain a matrix protein polyclonal antibody; using the monoclonal antibody to establish a method for testing high specificity and sensitivity; meanwhile, under the existence of different stimulating factors, testing the secretion change of the matrix protein of the pallium cells of the Pinctada martensi of primary culture. The invention provides a new method for researching the regulation and control of mineralized relevant matrix protein, thereby laying the foundation for the research on mineralized models.

The invention relates to a pharmaceutical composition for preventing and treating atherosclerosis as well as a preparation method and application method thereof. The composition comprises the following components: cyrtomium rhizome, rhizoma polygoni cuspidati, radix polygoni multiflori, radix salviae miltiorrhizae, white alum, radix angelicae sinensis, semen astragali complanati, radix ginseng, pericarpium granati, herba ephedra and cortex cinnamomi. The pharmaceutical composition containing the composition can obviously improve the blood lipid level of a New Zealand rabbit model with the atherosclerosis, reduce inflammatory factor release and relieve myocardial damage, and is suitable for prevention and treatment of the atherosclerosis.

A tissue engineering cartilage construction method using bone matrix gelatin which comprises, using cartilage cell for isolated culture or base material stem cell for isolated culture to evoke cartilage cell i.e. seed cell, fetching New Zealand rabbit or human embryon os longum and metaphysic trabecular bone to construct bone matrix gel BMG, inoculating the seed cells harvested through isolated culture onto cortex bone or cancellous bone BMG for extracorporal culture.