281results about How to "Suitable for on-site testing" patented technology

An off-resonance photoacoustic spectrometric detection and analysis device consists of a photoacoustic spectrometric module (8). The photoacoustic spectrometric module (8) comprises a light source module (2-1), a photoacoustic signal generation module (2-2), an optical power measurement module (2-3) and a fault diagnostic analysis module (2-4). Optical signals generated by an infrared incoherence light source in the light source module (2-1) are focused by an ellipsoidal reflector to form a focused light beam, the focused light beam is modulated into light of specific frequency and wavelength by chopping blades of an optical chopper and optical filters and then transmitted to the photoacoustic signal generation module (2-2), and the fault diagnostic analysis module (2-4) calculates each gas concentration according to to-be-tested gas photoacoustic signals generated by irradiation, diagnoses faults and alarms. The off-resonance photoacoustic spectrometric detection and analysis device can be used for quantitative detection of gases such as SF6, CF4, SO2F2, SOF2, SO2, SF4 and H2O and is applicable to onsite online monitoring.

An immune bead is made up of the magnetic carrier micro ball which combines at least one immune matching base. The micro ball is composed of the magnetic nm particle and the high molecular framework material which the core is the metal particle; the out of the core is high molecular framework, the out layer is the functional layer which can combines functional gene of different immune matching base. The manufacture method includes: the bead pretreatment, the bead activation, manufacture of the coupling antibody, closing the antibody with the confining liquid and purifying the immune bead. The detecting method is to detect the different things by the immunological response sandwich, the competition and the indirect method and set the control system on the testing board. The board is made up of the encrusting test paper, the coupling mat, the sample mat, the water suction mat, the coving film and the testing board outside calipers. It has the high sensitivity and accurate quantity; the regent is simple and cost low.

The invention discloses a method for rapidly detecting polybrominated diphenyl ether residue in a sample, particularly in textiles. The method is characterized by comprising the following steps: (1) the sample is crushed, dipped in base solution and ultrasonically extracted in water bath, and solid phase microextraction is employed to concentrate a target compound; and (2) polybrominated diphenyl ether is qualitatively and quantitatively determined by gas chromatography-mass spectrometry after thermal desorption. The method helps effectively solve the problem of matrix effect that fabric fibers have no solvent extraction, obtains a satisfactory result for the analysis of a selected target compound, has the advantages of low organic solvent consumption, rapid extraction speed, less interference from impurities, high sample recovery rate and the like, and can be applicable to rapidly detecting polybrominated diphenyl ether flame retardant residue in textiles.

The invention belongs to the field of nucleic acid detection and discloses a method for semi-quantitatively detecting pathogenic nucleic acid by adding internal control nucleic acid. Corresponding internal control is added in the whole process of extracting and amplifying target nucleic acid and testing by using a test paper, so that the internal control and a target segment are parallelly operated, and the semi-quantitative detection is performed finally through color development and intensity contrast of three strips, namely a detection line, an internal control line and a quality control line on the test paper. In the method, in the whole process of processing the target nucleic acid, the corresponding internal control is taken as a positive contrast, and false negative results due to links such as extraction, amplification or sample application errors are avoided in the processing of detecting by using the test paper. Meanwhile, by comparing color development intensity of the internal control line and a sample line and introducing the semi-quantitative function on the basis of the qualitative function of the immunochromatographic test paper to estimate the copy number of tested samples, the detection results are more detailed, accurate and reliable. The method has the advantages of convenient and quick operation and capacity of meeting the actual clinical requirement.

A combined device for rapidly and quantitatively detecting multiple tumor markers is characterized by being composed of 3 to 8 pieces of independent tumor marker test paper such as AFP, CEA, PSA, CA125, CA153, CA199, CA242 and NSE, a clamping shell special for the 3 to 8 pieces of test paper, and an immune layer result analysis and judgment recorder with built-in corresponding standard curves and the judgment method. The test paper can be combined to form the detection device with the 3 to 8 pieces of test paper according to the specific requirements. The standard curves and the judgment method for the 8 pieces of test paper are built in the immune layer result analysis and judgment recorder and used for conducting quantitative judgment on test results, and forecasting of various tumor occurrence conditions can be conducted on different types of combination of the test results through the judgment method. Detection is completed within 15 minutes to 20 minutes. The combined device for rapidly and quantitatively detecting the multiple tumor markers has the advantages of being combined in detection, easy and convenient to operate, rapid in response, accurate in result, suitable for on-site test and the like, and the requirements for more accurate, easier and move convenient clinical detection are met.

The invention relates to an undetached measurement device and method for transmission efficiency of an automotive transmission system. The device has the advantages of accurate measurement, high efficiency, easy manufacture, and high practical value. The technical scheme of the invention is that: the device consists of a first rotating drum, a second rotating drum, an eddy current dynamometer arm, an eddy current dynamometer, a rotating drum center shaft, and a coupler, wherein the undetached measurement device for the transmission efficiency of the automotive transmission system also comprises an eddy current machine arm dynamometer, an automotive driving force dynamometer, and a speed regulating motor; an automotive rear hook is connected with the automotive driving force dynamometer through a pull rod; and the automotive driving force dynamometer is fixed on a fixed pile. The device and the method have the advantages of solving the problems of undetached measurement of automobile engine dynamic property and economical efficiency, and realizing the rapid detection of the automobile engine dynamic property and economical efficiency detection station. By combining modern computer technology, the device and the method can realize rapid display of the automobile engine dynamic property and economical efficiency detection, and print the results on the spot.

The invention provides a pesticide residue detection micro-fluidic chip with a pre-stored reaction reagent. The pesticide residue detection micro-fluidic chip is characterized by comprising a micro-fluidic channel, a liquid sac pool, an extracting solution sac, a sample pool, a sample pool cover, a plurality of reagent pools and bio-reagents stored in the reagent pools. During detection, a to-be-detected sample just needs to be put into the sample pool of the chip, a reagent solution such as an extracting solution, an enzyme liquid, a color developing agent solution or a primer solution does not need to be prepared. The chip is concise to operate and suitable for field detection of pesticide residues and has an extremely important application value.

The invention relates to a method of applying Rayleigh waves in non-linear ultrasonic evaluation of surface damage of a metal material. The method comprises the following steps: 1) excitation and reception of Rayleigh waves; 2) detection of reliability of a testing system; 3) measurement of non-linear coefficients when the surface of a test piece has damage of different degrees; 4) repeatable operation of test. Compared to the method of using body waves for ultrasonic nondestructive test, the non-linear ultrasonic evaluation method provided in the invention has the characteristic that Rayleigh surface waves are especially suitable for measurement of non-linear coefficients of large-scale complex plate structures. According to the invention, it is only needed to carry out excitation and measurement of supersonic waves at one side of a structure during measuring, which enables measuring to be simple and easy; the Rayleigh surface waves have advantages favorable for measuring, e.g., concentration of energy on the surface of a structure, a long propagation distance, etc.; operation is simple and easy, so the method is especially suitable for on-site detection of structural elements; effective evaluation of early damage and degeneration of mechanical properties of a material structure can be realized by using the non-linear ultrasonic method.

The invention is applicable to the field of analytical chemistry and in particular relates to an identification method of ganoderma lucidum spore oil. The method is used for distinguishing the ganoderma lucidum spore oil from rapeseed oil, soybean oil, corn oil, sunflower seed oil, peanut oil and olive oil, and comprises the following steps: carrying out laser spectrum scanning on a sample to be detected to obtain a characteristic Raman spectrogram of the sample; comparing the Raman spectrogram with standard conditions, wherein the standard conditions are as follows: a displacement value at a first part is 1300+ / -3cm<1> and a displacement value at a second part is 1262+ / -3cm<1>, the Raman spectrum intensity values of the first part and the second part are more than 200, and the ratio of the Raman spectrum intensity values of the first part and the second part, and the ratio of signal peak areas of the first part and the second part are more than 2; and determining the content of ergosterol on a sample meeting the standards, wherein the sample with the content of the ergosterol of 0.10-2.79mg / g is the ganoderma lucidum spore oil. The identification method provided by the invention is simple and rapid, and has a wide application prospect in the aspect of rapid identification of the ganoderma lucidum spore oil and other common vegetable oil.

The invention relates to a device and a method for fruit and vegetable pesticide residues based on a laser Raman spectrometer. With the development of agricultural technology, various pesticides are used for prevention of fruit and vegetable pests in order to meet people's requirements in daily life by efficiently producing the fruits and vegetables with high yield, thus pesticide residues are formed on the surfaces of the fruits and vegetables unavoidably. The device for fruit and vegetable pesticide residues based on the laser Raman spectrometer comprises a display screen, a RS232 cable, a processor, a laser device, optical fibers, clamp nuts, a lens, a sample support, a probe housing, a reflective mirror, an optical fiber coupler, a pinhole, a focus lens, a collimating lens, optical gratings, a detector, a cut-off optical filter and a data line which are all fixed on the casing of the device. The device for fruit and vegetable pesticide residues based on the laser Raman spectrometer has the advantages of being compact in structure, low in power dissipation, high in stability, convenient to carry, and suitable for field detections. During detection, no movement of the device is needed, detection results are not affected by external vibrations, and the detection precision and speed are high.

The invention discloses a Salmonella LAMP (loop-mediated isothermal amplification) primer group and kit and a detection method, belonging to the field of a molecular biological detection method for food safety. The Salmonella LAMP primer group designed by the invention comprises a detection primer group and an internal standard primer group; and the detection kit comprises the Salmonella LAMP primer group, a DNA (deoxyribonucleic acid) polymerase, an LAMP reaction solution, a fluorescent nucleic acid dye, an internal standard, a positive control and a negative control. The detection method comprises the following steps: extracting a sample to be detected, and amplifying the sample template at 63-65 DEG C for 30-45 minutes. Thus, whether the sample to be detected contains Salmonella can be detected, and meanwhile, whether a false negative detection result exists can be judged according to whether the detection internal standard primer group is amplified. The invention has the advantages of high speed, high efficiency, simple operation process, high specificity, high sensitivity and the like, is suitable for on-site detection, can effectively prevent the occurrence of false negative, and is suitable for popularization and application.

The invention relates to a colloidal gold immunochromatography test strip used for testing an H1N1 influenza antigen and a method for testing the H1N1 influenza antigen. The test strip comprises a base plate, a sample pad, a conjugate pad, a pyroxylin film and an absorbent paper, wherein the sample pad, the conjugate pad, the pyroxylin film and the absorbent paper are lapped and adhered on the base plate sequentially; the conjugate pad is a combination of a gold-label-biotin conjugate pad and a streptavidin-gold-antibody conjugate pad; according to the method for testing the H1N1 influenza antigen, a sample to be tested is dropwise added on the test strip; if a red band is displayed at a testing line (1), the sample to be tested contains the H1N1 influenza antigen. According to the advantages, the test strip provided by the invention is cheap in price, fast, simple, convenient, and greatly suitable for on-site testing; moreover, the test strip can meet the requirement of higher testing sensitivity, and has an important clinical diagnostic significance.

The invention provides ketamine-collaurum test paper for detection of saliva. The ketamine-collaurum test paper comprises a reaction film and a gold mark cushion, wherein according to the reaction film, a reaction line and a quality control line are formed by respectively coating ketamine and quality-control secondary antibodies IgG (immunoglobulin G) connected with carrier protein on a nitrocellulose film (NC film); the gold mark cushion is coated with ketamine monoclonal antibodies marked with collaurum; and a film chromatography competitive inhibition method is adopted to detect the ketamine in a saliva sample. The test paper can be used for effectively monitoring the ketamine in saliva, the detection specificity is strong, the repeatability is good, the sensitivity is high, and the lowest detection quantity reaches 50ng / ml. The test paper is simple to operate, does not need special instruments and equipment, does not need professional training and has clear and obvious results, thus being suitable for field detection.

The invention relates to a portable elevator speed limiter detection device and a detection method for an elevator speed limiter. A singlechip system is used for controlling an alternating-current servo motor, a drive wheel is driven to rotate according to a set acceleration, and the drive wheel drives a wheel disc of the elevator speed limiter to be detected to rotate to ensure that a linear speed of a pitch circle of the wheel disk of the speed limiter gradually raises; and the singlechip is used for receiving a pulse signal of one magnetic steel at the end face of the wheel disc of the speed limiter through a Hall switch, and the diameter of the pitch circle of the wheel disc of the speed limiter is combined so that the linear speed of the pitch circle of the wheel disc of the speed limiter can be calculated. When the singlechip controls the alternating-current servo motor to continuously accelerate, the rotating speed of the wheel disc of the speed limiter is higher and higher; and finally, the speed limiter generates limit action due to centrifugal force action, the wheel disc is blocked, and the singlechip locks the linear speed at the instant action of the speed limiter, thus the linear speed is a measurement result. The portable elevator speed limiter detection device has the advantages of reasonable structure design, high efficiency and good effect, and is simple and quick in operation.

The invention provides a method for completely checking a pressure pipeline with a heat preservation function without stopping running. The method comprises the following steps of: (1) determining a key erosion detection measurement part of the pressure pipeline with the heat preservation function by using an infrared thermal imaging technology; and (2) detecting the part to be detected by using an x-ray real-time imaging technology. According to the method for completely checking the pressure pipeline with the heat preservation function without stopping running, the problem about detection for welding quality of a welded joint of the similar pipeline and selective examination and measurement for residual thickness of the pipeline is solved by using the infrared thermal imaging technology and the x-ray real-time imaging technology; therefore, the requirement of checking without stopping running can be met, and the work efficiency can be improved.

The invention discloses an immune colloidal gold test paper strip for detecting staphylococcus aureus enterotoxin A and a preparation method, comprising the construction of engineering bacteria of expression recombinant SEA protein, the separation and the purification of the SEA protein, the preparation of an anti-SEA specific antibody and the preparation of the SEA colloidal gold test paper strip. The test paper strip consists of a sample pad, a colloidal gold marker pad which is coated by a colloidal gold marker-labeled anti-SEA antibody and a cellulose nitrate film which is coated by an anti-SEA antibody and an anti-mouse IgG at a detection line and a quality control line; furthermore, the sample pad, the colloidal gold marker pad, the cellulose nitrate film and a water absorbent pad are sequentially adhered on a PVC bottom plate. The test paper strip has convenient, rapid and accurate operation, the whole process only needs 10 minutes and is not interfered by the environmental conditions, the specificity is good, the detection sensitivity is high and the lowest detection limit is 1ng / ml. The test paper strip is applicable to customs, hospitals and inspection quarantine institutions, etc., which can further realize the rapid detection of the staphylococcus aureus enterotoxin A in foods or in clinical samples.

The invention relates to a colloidal gold immunochromatographic test strip for detecting shrimp allergen and a preparation method thereof, belonging to the technical field of immunoassay. The invention discloses the assembled test strip which takes a combination of shrimp allergen-tropomyosin cluster specific antibody and color-developing substance colloidal gold as a detection reagent, the test strip is used for detecting the shrimp allergen in a food, and the test strip has low cost, fastness and portability. The applied antibody is an extracted and purified polyclonal antibody from a New Zealand rabbit with tropomyosin immune health. Due to the adoption of the polyclonal antibody, the test strip has low cost, good stability and good repeatability, and can be used in the detection of different samples.

The invention discloses genetically modified rice BT63, an LAMP (loop mediated isothermal amplification) detection primer group, a detection kit and a detection method, a detection kit and a detection method of the genetically modified rice BT63. The detection primer group comprises four specific primers; the detection kit comprises primer liquid, reaction liquid, DNA (deoxyribonucleic acid) polymerase, a reference and also a color reagent. The detection method comprises the steps that a DNA template of a sample is amplified at 63-65 DEG C by extracting DNA of rice variety of rice to be detected and adopting four specific primers and one DNA polymerase with strand displacement activity, wherein the amplification efficiency can achieve 109-1010 copies in a short time, and the identification is as follows: SYBRGreen I is added to observe the color change or a turbidity meter is utilized for observing the turbidity change of sediments in a reaction tube so as to judge whether amplification is conducted or not, so that whether the rice variety to be detected contains genetically modified rice BT63 and the derived variety thereof or is genetically modified rice BT63 and the derived variety thereof is ensured. The detection method provided by the invention has the advantages of rapidness, high efficiency, easiness and convenience in operation, high specificity, high flexibility, easiness and convenience in identification, and suitability of on-site detection, and is suitable for promotion and application.

The invention discloses an LAMP primer group and an LAMP detection kit for staphylococcus aureus and a use method of the detection kit. The LAMP primer group for staphylococcus aureus comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. According to the LAMP primer group, the LAMP detection kit and the use method, loop-mediated isothermal amplification primers are designed according to the sequences of the conservative regions of the heat-resistant nuclease genes (nuc) of staphylococcus aureus, the specific regions of target genes are amplified by adopting the LAMP technology, thus the rapid detection for staphylococcus aureus is realized from the molecular level, and an effective and rapid nucleic acid screening detection method is provided for detecting staphylococcus aureus.

The invention provides a direct determination method for the mercury content in natural gas. The determination method comprises the following steps of: (1) connecting determination devices, wherein the determination devices comprise a gasbag filled with the natural gas, a first three-way valve, a second three-way valve, a three-way pipe, a mercury filter and a mercury detector; and (2) firstly introducing the natural gas into the mercury detector and detecting to obtain a detected value C1, filtering out the mercury from the natural gas by using the mercury filter, then, feeding the filtered natural gas into the mercury detector and detecting to obtain a detected value C2, and obtaining the mercury content C in the natural gas according to an equation, i.e. C= C1-C2. The determination method provided by the invention has the advantages of high accuracy in obtained detection result, good repeatability, strong applicability, and no influence arising from oil content and moisture content, and is applicable to natural gases of various types, such as wellhead natural gas and the feed gas and product gas of a treatment plant.

The invention relates to a detection method for specific antibodies IgM of mycoplasma pneumonia and influenza viruses based on a micro-fluidic chip and application of the detection method. The method is characterized by taking the micro-fluidic chip with a plurality of pipelines as a reaction platform and detecting by using a chemical luminescent signal so as to independently or simultaneously detect the serum specific antibodies IgM of mycoplasma pneumonia and influenza viruses. As the micro-fluidic chip is combined with chemiluminiscence, the detection method integrates the advantages of rapidness, high efficiency and small sample amount of the micro-fluidic chip and high specificity of chemiluminiscence, also has the advantages of simplicity in operation, low cost, rapidness and the like, is a multi-target, real-time and high-sensitivity detection method and can be applied to rapid diagnosis of pneumonia and influenza.

The invention provides a method for preparing nanogold through a mango peel reducing agent. The method comprises the following steps: preparing a chloroauric acid solution; adjusting the pH value of the chloroauric acid solution; adding a mango peel reducing agent accounting for 2%-20% of the chloroauric acid solution according to volume ratio; performing water bath for 90-100 min under a certain condition; stopping the reaction when the color of the chloroauric acid solution turns into fuchsia from yellow; separating composite nanogold from the chloroauric acid solution through centrifugal separation; lastly, washing subnatant with distilled water and dehydrating; drying the the subnatant obtained through separation under a certain condition to obtain nanogold particles. According to the method, as the mango peels are prepared into as the reducing agent, the mango peels are changed from waste into valuable and the comprehensive utilization benefits of mangos are increased; compared with the prior art, the method has the advantages that steps are simple, the condition is mild, the preparing steps are simplified greatly and difficulties are reduced; no poisonous by-products are generated in the synthesizing processes, the yield of prepared nanogold particles can reach 90% above; the composite nanogold particles are even in sizes, narrow in particle size distribution, good in dispersity, low in possibility of gathering and more stable.

The invention discloses a method for detecting the content of benzoyl peroxide in flour. The method comprises the following steps of adding an organic solvent into flour, fully stirring, carrying out immersion to obtain benzoyl peroxide extract, carrying out centrifugation of the benzoyl peroxide extract to obtain supernatant liquid, putting the supernatant liquid into a colorimetric pipe, addinga potassium iodide solution into the colorimetric pipe, adjusting a pH value of the mixed solution in the colorimetric pipe to a pH value of 2 by strong acid, placing the colorimetric pipe in the shadow until a reaction is balanced; adding a starch indicator into the colorimetric pipe, adding water into the colorimetric pipe for dilution of the mixed solution in the colorimetric pipe, and comparing a color of the diluted solution in the colorimetric pipe with colors of a standard colourimetric card to determine the content of benzoyl peroxide. The method is easy for operation, does not need ahigh-priced apparatus, reduces a detection cost and detection time, has low requirements for detection persons and a detection environment, can realize on-site detection, and is suitable for on-site analysis and large-area screening work.

The invention discloses a LAMP detection primer, kit and detection method for staphylococcus aureus containing an internal standard. The primer is shown as SEQ ID NO 1-SEQ ID NO 12, and comprises a detection primer group and an internal standard primer group; and the detection kit comprises a primer group, a reaction solution, a DNA polymerase, positive control and an internal standard. According to the detection method, a sample to be detected is extracted, and a sample template is subjected to amplification for 45-60 minutes at the temperature of 63-65 DEG C, so that whether the sample to be detected contains the staphylococcus aureus can be detected, and whether a false negative detection result exists can be judged according to the fact that whether the internal standard primer group is subjected to amplification. The LAMP detection primer has the advantages of high rapidness, high efficiency, easy and convenient operation, high specificity, high sensitivity, suitableness for field detection and the like; and moreover, false negative detection can be effectively avoided, and the LAMP detection primer is suitable for popularization and application.

The invention dislcoses an RAA (recombinase-aid amplification) constant-temperature fluorescence detection method and a detection kit for EHP (enterocytozoon hepatopenaei). The detection kit comprisesa forward primer SEQ ID NO. 1, a reverse primer SEQ ID NO. 2, a specific fluorescence probe SEQ ID NO. 3, reaction liquid, recombinant polymerase and a reference substance. The kit has the advantagesthat the specificity is strong; the detection sensitivity is high, and can reach 100fg / mu L; high accuracy and reliability are achieved; the operation is simple and rapid, and the kit is suitable forfield detection and has wide application scenarios.

The invention belongs to the field of bio-detection and particularly relates to a C reactive protein saliva test paper strip and a preparation method thereof. The C reactive protein saliva test paper strip includes a reactive film and a gold marker pad. The reactive film is a nitrocellulose membrane, which has a detection line coated with a C reactive protein monoclonal antibody and a quality control line coated with goad-anti-mouse IgG. The gold marker pad is a polyester membrane that is coated with the C reactive protein monoclonal antibody marked by colloidal gold. The test paper strip, with human saliva as a detection sample, can effectively monitor the C reactive protein in saliva. The test paper strip has strong specificity and good repeatability, has high sensitivity, is 10 mg / L in lowest detection limit, is easy to operate and is free of special instruments and devices and professional training, has clear and distinguishable result and is easy to promote, and is suitable for on-site test.

The invention discloses an immunochromatographic test strip for rapid detection of tenuazonic acid. The immunochromatographic test strip is prepared from a hapten with a structure shown as formula (I). The invention adopts one-step indirect competitive immunochromatography technology to develop the immunochromatographic test strip for rapid qualitative or semi-quantitative detection of the TeA content in a sample, the immunochromatographic test strip is simple in operation, economical and practical, is suitable for on-site detection, and can be widely applied to detection and rapid screening of TeA contamination in agricultural products, food and other samples.

The invention discloses a method for detecting trace formaldehyde, and belongs to the technical field of analytical chemistry. The method for detecting trace formaldehyde comprises the following steps: by taking an extract of a solid sample or a filtrate of a liquid sample as a liquid to be detected, adding a derivatization reagent into the liquid to be detected, and deriving at room temperature so as to obtain a derivative product; adding a small amount of the derivative product into a reinforcing agent, and standing for minutes at room temperature; testing a raman spectrum within a range of 600-1800cm<-1> by using a portable type laser-raman spectrometer, and quantitatively detecting trace formaldehyde according to a regression equation between the formaldehyde concentration c and a peak area A which is obtained after the blank solution peak area is deducted at (832+ / -5)cm<-1>. The method disclosed by the invention is simple to operate, low in cost, good in reproducibility and high in sensitivity, the limit of quantification can be 1mu g / L, the detection on trace formaldehyde is achieved, and the method is applicable in on-site detection and easy to popularize, and has good application prospect.

The invention provides application of a cholesterol molecule imprinted membrane sensing electrode in detecting cholesterol content in blood. Specific steps in the method are as follows: (1) treating a blood sample, that is, standing the blood sample at room temperature and carrying out centrifugation to extract serum if the blood sample is obtained by drawing blood from a person who is on an empty stomach, or carrying out anticoagulation by using heparin or EDTA (ethylene diamine tetraacetic acid) if the blood sample is blood plasma; (2) dropwise adding the blood sample obtained in step (1) onto the cholesterol molecule imprinted membrane sensing electrode, inserting the cholesterol molecule imprinted membrane sensing electrode into an amperometric sensor for determination, and recording readings. When used for detecting cholesterol content in blood, the molecule imprinted membrane sensing electrode has the advantages of low cost, high sensitivity, fast analysis speed, capacity of reducing sampling amount and avoiding tedious pretreatment of blood samples, etc.

The invention provides an ethanol concentration detection kit and a manufacture method thereof. The ethanol concentration detection kin is composed of a reagent strip and a standard comparison card, the reagent strip is a filter paper strip with width ranging from 0.5cm to 1cm. Immobilized ethanol dehydrogenase, diaphorase, oxidized iodonitrotetrazolium (INT) and coenzyme are arranged on the filter paper strip. The standard comparison card comprises color developing comparison areas of ethanol with different concentrations. The detection kit is convenient, sanitary, fast and high in sensitivity and stability when being used for detecting ethanol concentration of the drunks, and the detection kit is easy to carry and particularly suitable for on-site detection.