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91 results about "Displacement activity" patented technology
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Displacement activities occur when an animal experiences high motivation for two or more conflicting behaviours: the resulting displacement activity is usually unrelated to the competing motivations. Birds, for example, may peck at grass when uncertain whether to attack or flee from an opponent; similarly, a human may scratch their head when they do not know which of two options to choose. Displacement activities may also occur when animals are prevented from performing a single behaviour for which they are highly motivated. Displacement activities often involve actions which bring comfort to the animal such as scratching, preening, drinking or feeding.
Isothermal chimeric primer nucleic acid amplification methods using blocking oglionucleotide
InactiveUS7056671B2Improve targeting efficiencyHigh detection sensitivityMicrobiological testing/measurementFermentationPolymerase LRibonucleotide synthesis
Methods of amplifying a target nucleic acid whereby the target nucleic acid is amplified in the presence of a deoxyribonucleotide triphosphate, a DNA polymerase having strand displacement activity, at least one chimeric oligonucleotide primer, at least one upstream block oligonucleotide and a RNase H; wherein the chimeric oligonucleotide primer contains a ribonucleotide positioned at the 3′-terminus; wherein the upstream block oligonucleotide is capable of annealing to a region 3′ to a portion in the nucleic acid as the template to which the chimeric oligonucleotide primer anneals; and compositions and kits thereof.
Owner:TAKARA HOLDINGS
Method for amplification of nucleotide sequence
InactiveUS20100055742A1Increase costSugar derivativesMicrobiological testing/measurementNucleotidePolymerase L
Owner:NISHIKAWA RUBBER
Method for synthesis of double-stranded DNA corresponding to rna, and method for amplification of the DNA
InactiveUS20120058520A1Easy and cheap gene analysisSimple and cheap identificationFermentationDNA preparationNucleotidePolymerase L
An object of the present invention is to provide an inexpensive and simple method for synthesis of a double-stranded DNA corresponding to a particular RNA, and a method for amplification of the aforementioned double-stranded DNA. The present invention relates to a method for synthesis of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, comprising step 1 in which reverse transcription reaction of template RNA is carried out employing oligo(dT)primer to which DNA fragment having a known sequence has been added at the 5′-terminal, to obtain a single-stranded DNA, and step 2 in which double strand formation reaction of single-stranded DNA obtained in step 1 is carried out employing a random primer to which DNA fragment having a known sequence has been added at the 5′-terminal, in the presence of polymerase which does not have 3′→5′ exonuclease activity nor strand displacement activity, to obtain a double-stranded DNA, as well as a method for amplification of a double-stranded DNA having a nucleotide sequence corresponding to template RNA having polyA, further comprising step 3 in which PCR reaction is carried out using the obtained double-stranded DNA.
Owner:WAKO PURE CHEMICAL INDUSTRIES
Isothermal nucleic acid amplification method using surfactant
ActiveUS8435741B2Simpler primer designEfficient amplificationMicrobiological testing/measurementFermentationPolymerase LOligonucleotide Primer
Owner:FUJIFILM CORP
Thermostable viral polymerases and methods of use
ActiveUS8093030B2High known fidelityReduce measurementSugar derivativesMicroorganismsNucleotideReverse transcriptase activity
Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and / or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.
Owner:LUCIGEN
Nucleic acid amplification method
InactiveUS20090155856A1Improve efficiencyImprove efficacyMicrobiological testing/measurementFermentationNucleotideOligonucleotide primers
An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified using oligonucleotide primers and DNA polymerase. The present invention provides a nucleic acid amplification method which comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment, wherein a first oligonucleotide primer and a second oligonucleotide primer are designed in such a way that a region which contains two identical sequences X of serial 4 or more nucleotides within, the region of 200 or less nucleotides, or apart thereof can be amplified.
Owner:FUJIFILM CORP
Nucleic acid amplification method
ActiveUS20090170096A1Simpler primer designEfficient amplificationMicrobiological testing/measurementFermentationNucleic acid detectionOligonucleotide primers
An object to be achieved by the present invention is to provide a nucleic acid amplification method by which a nucleic acid can be amplified substantially isothermally using oligonucleotide primers and DNA polymerase capable of strand displacement. The present invention provides a nucleic acid amplification method which comprises performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, at least 0.01% or more surfactant, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3′ end of the primer and thus amplifying the nucleic acid fragment.
Owner:FUJIFILM CORP
Primer-type nucleic acid fluorescent probe subjected to two-way strand displacement
ActiveCN105803074ASimple structureHigh indication sensitivityMicrobiological testing/measurementDNA/RNA fragmentationFluorescenceSingle strand
The invention provides a primer-type nucleic acid fluorescent probe subjected to two-way strand displacement.The primer-type nucleic acid fluorescent probe is composed of two oligonucleotide strands of which 5'-end sequences are completely complementary, the middle of the primer-type nucleic acid fluorescent probe is a double-strand zone, and the two ends of the primer-type nucleic acid fluorescent probe are provided with single-strand arms respectively.The probe is simple in structure and reasonable in design and can simultaneously serve as an amplification primer and a signal probe in an amplification reaction, chemical modification to the 3' end is not needed, and the process of additionally designing a probe is avoided.According to the probe, the strand displacement activity of nucleotide polymerase is utilized, high-sensitivity real-time fluorescence detection on nucleic acid isothermal amplification such as isothermal multiple-self-matching-initiated amplification can be achieved, visual bifluorescence product detection can be constructed by combining ion indicators such as hydroxynaphthol blue, the potential for constructing single-tube multiple nucleic acid isothermal amplification technology is achieved, and a novel nucleic acid fluorescent probe is provided for diagnosis or detection research of related markers in biology, medicine, chemistry and the like.
Owner:ZHEJIANG UNIV
Dna Polymerases Having Strand Displacement Activity
InactiveUS20080311626A1Rapid efficientMaintain good propertiesBacteriaSugar derivativesBacteroidesPolymerase L
The invention provides novel strand displacement DNA polymerases which can be used in a rapid and efficient strand displacement amplification reactions. The polymerases are significantly more thermostable than prior art polymerase and retain high activity at elevated temperatures. Also disclosed are genes encoding the polymerases and vectors comprising the genes. Representative polymerases of the invention are obtainable from bacterial strains of the species Thermus antranikianii and Thermus brockianus and also from environmental samples with isolation of the source species.
Owner:ARKEA HF
Nucleic acid amplification method
InactiveUS20100255546A1Inexpensive and simple isothermal nucleic acid amplification methodSimplify the design processMicrobiological testing/measurementFermentationPolymerase LA-DNA
Disclosed is a novel isothermal nucleic acid amplification method enabling inexpensive and simple and easy detection. The method includes introducing nicking enzyme recognition sequences into an analysis target nucleic acid using nicking enzyme recognition sequence-containing primers and isothermally amplifying a specific region of the target nucleic acid using the primers, a nicking enzyme and a DNA polymerase having strand displacement activity.
Owner:HITACHI LTD
Method for rapidly detecting pig source components in red meat through real-time fluorescence loop-mediated isothermal amplification (LAMP) method
InactiveCN103243165AHigh sensitivityStrong specificityMicrobiological testing/measurementFluorescence/phosphorescenceFluorescenceLoop-mediated isothermal amplification
The invention discloses a method for rapidly detecting pig source components in red meat through a real-time fluorescence loop-mediated isothermal amplification (LAMP) method. According to the method, the pig specific cytochrome b genes in the red meat are detected by employing a method for combining the LAMP technology with real-time fluorescence. According to the detection method, the genome DNA of the meat to be detected is extracted, six specific primers and Bst DNA polymerase fragments with strand displacement activity are amplified at the temperature of 60-65 DEG C for 45-60 minutes, SYBR GREEN I is added into the reaction system, and the amplification conditions of the sample template can be detected in real time, so that whether pig source components are contained in beef and mutton samples can be judged. The method has the advantages of high sensitivity, high specificity, simplicity and rapidness, and the result is not required to be subjected to gel electrophoresis.
Owner:长沙市食品质量安全监督检测中心 +1
Thermostable Viral Polymerases and Methods of Use
ActiveUS20080268498A1Improve stabilityIncrease rangeSugar derivativesMicroorganismsNucleotidePolymerase L
Thermostable viral polymerases exhibiting a combination of activities selected from, proofreading (3′-5′) exonuclease activity, nick translating (5′-3′) nuclease activity, synthetic primer-initiated polymerase activity, nick-initiated polymerase activity, reverse transcriptase activity, strand displacement activity, and / or decreased discrimination against incorporation of nucleotide analogs. Also provided are compositions including the polymerases, polynucleotides encoding the polymerases and methods of using the polymerases.
Owner:LUCIGEN
Nucleic acid amplification method
ActiveCN101368197AHigh amplification efficiencyMicrobiological testing/measurementFermentationOligonucleotide primersNucleotide
The present invention provides a nucleic acid amplification method by using a oligonucleotide primer and a DNA polyase. The nucleic acid amplification method provided by the invention comprises performing incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, at least two types of oligonucleotide primer, and the nucleic acid fragment as a template so as to perform a polymerase reaction that initiates from the 3' end of the primer and thus amplifying the nucleic acid fragment, wherein the characteristic is that a first oligonucleotide primer and a second oligonucleotide primer are designed in such a way that a region which contains two identical sequences X of serial 4 or more nucleotides within the region of 200 or less nucleotides, or a part thereof can be amplified.
Owner:FUJIFILM CORP
Solid phase isothermal amplification
ActiveUS20170137874A1Microbiological testing/measurementFermentationPolymerase LReaction temperature
Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
Owner:GLOBAL LIFE SCI SOLUTIONS OPERATIONS UK LTD
LAMP detecting primer group, kit and detecting method for cry3A gene in transgenic plant
InactiveCN103924000AHigh sensitivitySimple and fast operationMicrobiological testing/measurementDNA/RNA fragmentationTurbidityColor changes
The invention discloses an LAMP detecting primer group, a kit and a detecting method for a cry3A gene in transgenic plant. The primer group consists of 5 specific primers with nucleotide sequences as shown in SEQ ID NO:1-5. The detecting kit comprises detecting primer liquor, loop primer liquor, DNA polymerase with strand displacement activity, 10*reaction buffer liquor, dNTPs liquor, positive DNA contrast and negative DNA contrast. The kit further contains a color developing agent. The detecting method adopts the specific primers and the DNA polymerase with the strand displacement activity to carry out amplification onto a sample DNA template at 63 DEG C-65 DEG C, and adopts a method of adding the color developing agent to observe color changes or to directly observe turbidity change of precipitates in a reaction tube, and the like, to judge whether the sample contains the cry3A gene component or not. The LAMP detecting primer group, kit and detecting method disclosed by the invention do not need a special apparatus, have the characteristics of being quick and efficient, simple and convenient to operate, strong in specificity, high in sensitivity, and the like, and are suitable for site detection.
Owner:JILIN ACAD OF AGRI SCI
LAMP primer group, kit and detection method for rapid test of bacterial polymyxin drug resistance gene mcr-1
InactiveCN106834506AAccurate identificationStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationPolymixin EPolymerase L
The invention discloses a LAMP primer group, a kit and a detection method for rapid test of a bacterial polymyxin drug resistance gene mcr-1. The kit comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The six specific primers and a DNA polymerase with strand displacement activity are adopted for amplification of a DNA sample at 60-65 DEG C, and color changes in a reaction tube can be observed to judge whether amplification occurs or not after addition of a color developing agent. The LAMP primer group, the kit and the detection method have advantages of quickness, high efficiency, simplicity and convenience in operation, high specificity, high sensitivity, simplicity and convenience in detection, suitableness for onsite detection and the like and is suitable for popularization and application.
Owner:SUN YAT SEN UNIV
Transgenic rice Bt63 detection kit and detection method thereof
InactiveCN102618661ASuitable for self-testStrong specificityMicrobiological testing/measurement3-deoxyriboseFluorescence
The invention discloses a transgenic rice Bt63 detection kit and a detection method thereof, relating to transgenic rice. The kit comprises a loop mediated isothermal amplification reaction liquid A, a loop mediated isothermal amplification reaction liquid B, a Bst DNA (Deoxyribose Nucleic Acid) polymerase C and a color developing agent D. The method comprises the following steps of: extracting template DNA; performing loop mediated isothermal amplification; and performing color developing detection. Four specially-designed primers and the Bst DNA polymerase with strand displacement activity are used for specially, efficiently and rapidly amplifying DNA under a constant-temperature condition, so that 109 target sequence coping can be realized within one hour. The amplification effect can be observed through a fluorescent dye. A rapid loop mediated isothermal amplification method with high specificity and low cost is used for detecting Bt63 transgenic rice, and can be used for replacing a CRL (Chemical Research Laboratory) method confirmed by the Joint Research Center, and novel scientific bases are provided for food safety supervision.
Owner:XIAMEN UNIV
Method for rapid detection of transgenic rice line TT51-1
InactiveCN103773836ASimple and fast operationStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationGenetically modified riceDirect observation
The invention discloses a method for rapid detection of transgenic rice line TT51-1 and primers for use. The method belongs to the technical field of molecular biology, and relates to detection methods of transgenic products, the principle is as follows: using 6 specific primers and a DNA polymerase with chain displacement activity for amplification of a sample template DNA at 61 DEG C, amplified products can reach 109-1010 copies in a short period of time, result identification can be realized as follows: determining whether the DNA is amplified or not by direct observation of color change in a reaction tube by naked eyes, and the method has the characteristics of being high in efficiency, fast, simple in operation, high in specificity and the like, and provides a convenient means for the rapid detection of transgenic rice.
Owner:TIANJIN INSTITUE OF QUALITY STANDARD & TESTING OF AGRICULTUAL PRODS
Kit and method for detection of transgenic maize Mon89034 and derivative varieties thereof through gene amplification at constant temperature
InactiveCN102776268AStrong specificityHigh sensitivityMicrobiological testing/measurementTurbidityGene
The invention discloses a kit and a method for detection of transgenic maize Mon89034 and derivative varieties thereof through gene amplification at a constant temperature. The kit mainly comprises four primers, DNA polymerase, a reaction solution, a positive contrast and a negative contrast, wherein all the above-mentioned liquid are respectively disposed in a container; the kit also comprises a color-developing agent. The detection method is as follows: DNAs of a maize variety to be detected are extracted, the four specific primers and the DNA polymerase having strand displacement activity are used for amplification of a sample DNA template at a temperature of 63 to 65 DEG C, and amplification efficiency reaches 109 to 1010 copies in a short period of time; for identification of the maize variety to be detected, SYBR Green I is added and changes of colors are observed, or changes of turbidity of deposition in a reaction tube are observed by using a turbidity meter so as to determine whether amplification occurs or not, and then detection is carried out on the maize variety to be detected so as to determine whether the maize variety contains or is transgenic maize Mon89034 and a derivative variety thereof. The beneficial effects of speed, high efficiency, simple operation, high specificity, high sensitivity, easy identification, suitability for on-site detection and the like are obtained in the invention.
Owner:广州迪澳生物科技有限公司
LAMP detection primer group of cry2Ab gene in transgenic crop and detection kit as well as detection method
InactiveCN103773867AHigh sensitivitySimple and fast operationMicrobiological testing/measurementDNA/RNA fragmentationPolymerase LTurbidity
The invention discloses an LAMP detection primer group of a cry2Ab gene in a transgenic crop and a detection kit as well as a detection method. The LAMP detection primer group comprises six specific primers, and the nucleotide sequences of the specific primers are as shown in SEQ ID NO: 1-6. The detection kit comprises a primer solution, DNA (deoxyribonucleic acid) polymerase with strand displacement activity, 10* reaction buffer liquid, dNTPs solution, positive DNA control and negative DNA control and can aslo contain a color developing agent. The detection method is characterized in that methods of using the specific primers and the DNA polymerase with the strand displacement activity for amplifying a sample DNA template at the temperature of 63-65 DEG C, adding the color developing agent to observe change in color or directly observe change in turbidity of precipitants in a reaction tube and the like are used for judging whether a sample contains the cry2Ab gene. The method does not need special instruments and has the characteristics of fastness, high efficiency, simplicity and convenience to operate, strong specificity, high sensitivity and the like and is suitable for field test.
Owner:JILIN ACAD OF AGRI SCI
An endonuclease VIII activity measuring method
ActiveCN104313133AEasy to operateGood repeatabilityMicrobiological testing/measurementPolymerase LRadioactive contamination
An endonuclease VIII activity measuring method is disclosed. The method includes: a step of synthesizing a primer according to a single-strand DNA template, wherein the primer comprises two parts which comprise a normal primer part at the 5' end and an end-capped primer part at the 3' end, and the two parts are connected by dUTP; a step of annealing the synthesized primer and the single-strand DNA template to form a composite; a step of generating an apyrimidinic site on the dUTP position of the primer under the functions of uracil-DNA glycosylase (UDG) and endonuclease VIII (Endo VIII); a step of synthesizing double-strand DNA by utilization of a polymerase with strand displacement activity; a step of measuring the relative amount of the double-strand DNA and single-strand DNA in a reaction system; and a step of deriving the degree of the endonuclease VIII activity according to the measurement result, wherein the degree of the endonuclease VIII activity is positively correlated with the amount of the double-strand DNA in the reaction system. Compared with the prior art, the method is free of radioactive contamination, simple and convenient in operation and capable of performing quantitative detection analysis on the Endo VIII activity.
Owner:VAZYME BIOTECH NANJING
Isothermal amplification under low salt condition
ActiveUS20150275282A1Expand coverageMicrobiological testing/measurementTransferasesReaction temperaturePolymerase L
Provided herein are methods and kits for isothermal nucleic acid amplifications that use a target nucleic acid template; a reaction mixture comprising a DNA polymerase having a strand displacement activity, a deoxyribonucleoside triphosphate (dNTP) mixture, a primer with a 3′ end and a 5′ end, a molecular crowding reagent, and a buffer solution for amplifying the target nucleic acid template. The buffer solution maintains a low salt concentration of the reaction mixture, and wherein the salt concentration results in a melting temperature (Tm) of the primer at least 10° C. below the reaction temperature. The amplification is effected under isothermal condition.
Owner:GLOBAL LIFE SCI SOLUTIONS OPERATIONS UK LTD
RT-LAMP (reverse transcription and loop-mediated isothermal amplification) detection primer group, RT-LAMP detection kit and RT-LAMP detection method for simian immunodeficiency virus
ActiveCN106636458AStrong specificityHigh sensitivityMicrobiological testing/measurementMicroorganism based processesPositive controlReverse transcriptase
The invention discloses an RT-LAMP (reverse transcription and loop-mediated isothermal amplification) detection primer group, an RT-LAMP detection kit and an RT-LAMP detection method for a simian immunodeficiency virus. The detection primer group comprises a pair of outer primers, a pair of loop primers and a pair of inner primers. The detection kit comprises the primer group, RT-LAMP reaction liquid, Bst DNA polymerase, reverse transcriptase as well as negative control and positive control. The detection method comprises the following steps: extracting to-be-detected virus RNA; conducting reverse transcription on the RNA under the action of revertase; then, amplifying a sample template by virtue of six specific primers and one Bst DNA polymerase having strand displacement activity at 63-65 DEG C; and judging whether a to-be-detected sample contains the simian immunodeficiency virus (SIV) RNA by observing whether a reaction tube solution becomes muddy or not with naked eyes or performing electrophoresis to analyze whether a ladder exists or not. The detection primer group, the detection kit and the detection method provided by the invention have the advantages of being highly specific, high in sensitivity, rapid and efficient, simple and convenient to operate, easy in result reading and the like, and are suitable for popularization and application in grassroots animal epidemic disease monitoring units and experimental monkey breeding enterprises in remote areas.
Owner:GUANGDONG LAB ANIMALS MONITORING INST
Method for synthesis of double-stranded DNA corresponding to RNA, and method for amplification of the DNA
Owner:WAKO PURE CHEMICAL INDUSTRIES
Method for rapidly detecting cow and sheep derived components
InactiveCN101724701ASimple and fast operationStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationTurbidityMicrobiology
The invention discloses a method for rapidly detecting cow and sheep derived components. The principle is that a cow-derived forward outer primer COW-F3, a cow-derived backward outer primer COW-B3, a cow-derived forward inner primer COW-FIP, a cow-derived backward inner primer COW-FIP, a sheep-derived forward outer primer SHEEP-F3, a sheep-derived backward outer primer SHEEP-B3, a sheep-derived forward inner primer SHEEP-FIP, a sheep-derived backward inner primer SHEEP-BIP and DNA polymerase with the chain displacement activity are used for amplifying nucleic acid at 63-65 DEG C with the amplification efficiency up to 109-1,010 copies in a short time. The identifying method is to directly observe the turbidity of precipitation in a reaction tube by using naked eye or judge that amplification is carried out or not by the color change of added SYBR Green. The detection method has the characteristics of high specificity, high efficiency, rapidness, simple operation, easy detection and the like.
Owner:CENT LAB TIANJIN ACADEMY OF AGRI SCI
DNA polymerases
The technology provided herein relates to novel variants of DNA-Polymerases exhibiting high termo-stability as well as a strong strand displacement activity; to nucleic acid molecules encoding said DNA-Polymerases, vectors, host cells containing the nucleic acids and methods for preparation and producing such enzymes; compositions comprising at least one of the DNA-Polymerases; and methods for using such enzymes in DNA sequencing and / or DNA amplification processes.
Owner:BIORON
RNA detection method
InactiveUS20090162856A1Short timeRapid and convenient mannerMicrobiological testing/measurementFermentationReverse transcriptasePolymerase L
It is an object of the present invention to provide a method for rapid, convenient, and highly sensitive detection of trace RNA wherein a risk of contamination is low. The present invention provides a method for amplification of nucleic acid which comprises the steps of: (i) allowing a reverse transcriptase to act on RNA so as to produce a nucleic acid fragment; and (ii) performing substantially isothermal incubation of a reaction solution containing at least one type of deoxynucleotide triphosphate, at least one type of DNA polymerase having strand displacement activity, a divalent cation, a surfactant accounting for at least 0.01% of the solution, at least two types of oligonucleotide primers, and the nucleic acid fragment as a template obtained in the step (i) so as to perform a polymerase reaction that is initiated from the 3′ ends of the primers and thus amplify the nucleic acid fragment.
Owner:FUJIFILM CORP
Method for amplifying genomic DNA
InactiveUS20090311753A1Spatial separationMaintain structural integritySugar derivativesMicrobiological testing/measurementBiotechnologyGenomic DNA
A method for amplifying genomic DNA is provided. The method comprises the steps of: (1) incubating a cell-containing agarose solution at a pH of 9 to 12 and a temperature of 45 to 80° C. to produce a genomic DNA-dispersed agarose solution wherein 0.002 to 1 copies / 5 microliter of single-stranded genomic DNA is dispersed; (2) solidifying the genomic DNA-dispersed agarose solution to produce a genomic DNA-dispersed agarose gel and neutralizing a pH of the gel; and (3) adding a DNA polymerase with strand displacement activity, primer and dNTP to the genomic DNA-dispersed agarose gel and incubating the gel at a temperature of 0 to 60° C. to amplify the genomic DNA.
Owner:NAT INST OF RADIOLOGICAL SCI
Identification of genetic modifications
ActiveUS20190040457A1Minimize side effectsSugar derivativesMicrobiological testing/measurementSide effectNucleotide
Described are methods of detecting modified nucleotide bases in a DNA sample using specific DNA glycosylases to excise target modified bases. DNA molecules are then labeled using a DNA polymerase lacking 3′→5′ exo-nuclease activity and strand displacement activity. The methods can be used to detect epigenetic changes and DNA damage. Provided are methods for diagnosing a disease or condition, determining risk of a disease or condition, identifying appropriate treatment, monitoring effectiveness of treatment, and monitoring side effects of treatment in subjects based on detection of modified bases. Also provided are methods for determining environmental exposure, or an environmental exposure time, of a biological sample containing DNA. Also provided are kits, systems, and devices for performing the described methods.
Owner:WAKE FOREST UNIV HEALTH SCI INC
LAMP (Loop-mediated isothermal amplification) detection primer group and kit and detection method for gene cry1C of transgenic plants
InactiveCN105483236ASimple and fast operationStrong specificityMicrobiological testing/measurementDNA/RNA fragmentationNucleotideLoop-mediated isothermal amplification
The invention discloses an LAMP (Loop-mediated isothermal amplification) detection primer group and kit and a detection method for a gene cry1C of transgenic plants. The primer group consists of 6 specific primers and has nucleotide sequences shown in SEQ ID NO: 1-6. The detection kit comprises a detection primer solution, DNA polymerase with strand displacement activity, a 10x reaction buffer, a dNTPs solution and a color developing agent. The detection method comprises the steps of carrying out amplification on a DNA template of a sample at the temperature of 63-65 DEG C by adopting the specific primers and the DNA polymerase with strand displacement activity, and judging whether the sample contains a gene cry1C component or not by adopting a color developing agent added color change observing method. The detection primer group and kit and the detection method do not need special instruments, have the characteristics of rapidness, high efficiency, simplicity and convenience in operation, high specificity and the like and are applicable to field detection.
Owner:JILIN ACAD OF AGRI SCI
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