106 results about "Polymixin E" patented technology

The invention relates to a compound microbial agent for degrading antibiotic and pesticide residues as well as preparation and application thereof, and belongs to the field of biotechnology and environmental protection. Multi-thallus compound microbial powder is prepared from the compound microbial agent according to the weight percentage of living microbes to the total amount of compound microbial powder as follows: 10%-15% of bacillus subtilis, 10%-15% of aspergillus niger, 10%-15% of bacillus mucilaginosus, 10%-15% of enterococcus faecalis, 8%-12% of bacillus licheniformis, 8%-12% of bacillus megaterium, 8%-12% of pseudomonas fluorescens, 5%-8% of lactobacillus plantarum, 5%-8% of bacillus polymyxin and 6%-8% of streptococcus thermophiles. The compound microbial agent has the effects of degrading antibiotic and pesticide residues, fermenting and composting organic matter, acting as functional fertilizer and repairing the environment, and can solve the problems of secondary pollution caused by antibiotic residues in culture feces and resource utilization of organic waste and realizes biodegradation of the antibiotic and pesticide residues in soil when applied to the agricultural ecological environment, thereby being of great value and practical significance in restoration of agricultural ecological environment and protection of human health.

The present invention provides a new class of polymyxin derivates useful for treating bacterial infections, especially Gram-negative infections, that have reduced renal cytotoxicity.

Provided herein are novel compounds and novel protected compounds that can be derived from polymyxin, including, e.g., polymyxin A. The novel compounds have antibacterial properties against a diverse range of Gram negative bacteria and reduced toxicity compared to polymyxins such as polymyxin A. Also provided are antibacterial pharmaceutical compositions containing the novel compounds and novel protected compounds, as well as methods for preparing the antibacterial compounds and protected compounds.

Provided herein are novel compounds and novel protected compounds that can be derived from polymyxin, including, e.g., polymyxin B1 and [Il7] polymyxin B1. The novel compounds have antibacterial properties against a diverse range of gram negative bacteria and reduced toxicity compared to polymyxins such as polymyxin B. Also provided are antibacterial pharmaceutical compositions containing the novel compounds and novel protected compounds, as well as methods for preparing the antibacterial compounds and protected compounds.

A pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent, wherein the enhancer of an antimicrobial agent is an inhibitor of gene, that by inactivating the gene product potentiates the effectiveness of the antimicrobial agent. In some embodiments, the pharmaceutical composition further comprises a pharmaceutically acceptable carrier. In some embodiments, the antimicrobial agent is an antimicrobial peptide such as a polymyxin, for example but not limited to colistin. In some embodiments of the present invention provides methods to treat and / or prevent infection of a subject with a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent. In some embodiments, the present invention provides methods to inhibit growth of a microorganism by administering a pharmaceutical composition comprising an antimicrobial agent and an enhancer of an antimicrobial agent.

The invention relates to a method for separating and purifying polymyxin E methanesulfonic sodium from a mixture containing small-molecular weight impurities. The method adopts an ultrafiltration membrane with the cutoff molecular weight ranging from 1,000 to 10,000 to carry out separation and purification. The invention also relates to a method used for preparing high-purity E methanesulfonic sodium by controlling reaction conditions and making use of ultrafiltration membrane separation technology. The method comprises the following steps: (a) adding water to dissolve polymyxin E sulfate andthen adding formaldehyde solution in the solution to carry out reaction; (b) adding sodium bisulfite solution in the reaction products of step (a) to carry out reaction continuously; and (c) taking the reaction solution of step (b) and adopting the ultrafiltration membrane to carry out separation and purification. The method of separating and purifying the polymyxin E methanesulfonic sodium has the advantages of low pollution, low energy consumption, simple process, good repeatability and high purity and activity of obtained products.

An endotoxic adsorpting method for treating endotoxemia and anaphylactic reaction caused by endotoxemia is prepared through activating the hydroxy group of agarose gel or DVA as carrier by cyanogen bromide or epoxy chloropropane or sulfuryl chloride, and adding polymyxin solution.

The invention provides a method for separately preparing for polymyxin E, which uses foam separation method to extract and separate the polymyxin E from fermentation liquor. The fermentation liquor obtains polymyxin E raw medicine by first treatment, form separating, vacuum evaporation and spray drying.

The invention discloses a polymyxin derivative and a preparation method thereof. Since zymohydrolysis, fatty acid chain on the end N of polymyxin B and one diaminobutyric acid residue are removed, a 3, 4-oxamido-benzoyl group is introduced through a chemical decoration technology or a benzene ring group with two fatty acid chains is introduced, so the polymyxin derivative is obtained. The derivative can be used for the clinical treatment of infection of Gram-negative multidrug resistance bacteria.

The combination of a polymyxin composition and a hop composition produces unexpected and synergistic activity against gram positive bacteria.

The invention discloses a process for extracting colistin sulfate through a precipitation method. The process comprises the following steps of: (a) adjusting pH of fermentation liquor containing polymyxin E with oxalic acid or concentrated sulfuric acid, and filtering with a filtering device; (b) adding 0 metal complexing agent and inorganic flocculant to the filtrate, and reacting at normal temperature for 0.5 to 4.0h; (c) heating, cooling, and vacuum-filtering the reactive liquid; (d) adjusting pH of the filtrate above with aqueous alkali, separating the solid from the liquid to obtain a filter cake, dissolving the filter cake with sulfuric acid solution so as to obtain acidizing fluid, adding potassium permanganate and activated carbon to the acidizing fluid respectively, agitating for0.5 to 2.0 hours, and then vacuum-filtering to obtain colistin sulfate liquid; and (e) centrifugally spray-drying the colistin sulfate liquid. With the adoption of the process, the common problems ofthe existing process for purifying fermentation liquor that the steps are complicated, the period is long, the labor intensity is relatively high, and the wastewater is difficult to treat and the like can be overcame.

The present invention relates to color developing culture medium, detecting kit and detection method for Vibrio parahaemolyticus, and belongs to the field of food microbe safety monitoring technology. The detecting kit consists of color developing culture medium comprising bacterial specific enzyme in 0.05-0.95 g and mixed color developing substrate Y-glucoside, enrichment liquid A of pancreatic casitone liquid culture medium and enrichment liquid B comprising sodium chloride, polymyxin and broth. The detection method includes the enrichment culture of the sample in enrichment liquid A and enrichment liquid B, the culture of the two parts of enrichment cultured liquid in the color developing culture medium, and judging whether to exist Vibrio parahaemolyticus according to whether to have smooth raised purple colony of 2-3 mm diameter. The present invention has low cost, high detection sensitivity, short detection period and other advantages.

The invention provides a pidotimod-containing pharmaceutical composition and a preparation method thereof. The pidotimod-containing pharmaceutical composition consists of pidotimod and any antibacterial medicament. The antibacterial medicament may be any of beta-lactams (including as penicillins, cephems and unclassical beta-lactams), aminoglycosides, polymyxins, macrolides, lincomycins, norvancomycins, tetracyclines, chloromycetins, artificial antibacterial medicaments (including sulfonamides, quinolones and furofurans), cholesterols and antiviral medicaments. The unit dosage of the pidotimod is 0.1 to 2 grams, preferably 0.4 to 0.8 gram. The dosage of the antibacterial medicament is determined according to the dosage range of the antibacterial medicament. The composition of the pidotimod and the antibacterial medicament can exogenously and directly kill pathogenic bacteria through the antibacterial medicament and mobilize endogenous anti-infection immunity through an immunomodulator to improve anti-inflection effect, and has excellent synergy.

The present invention relates to a polymyxin derivative wherein R1, R2 and R3 are optional and R1, R2, R3, R5, R8 and R9 are cationic or neutral amino acid residues selected so that the total number of positive charges at physiological pH is at least two but no more than three; and to a combination product comprising at least two such derivatives. The invention further relates to a method for treating, alleviating or ameliorating an infection in a subject, caused by a Gram-negative bacterium by administering a therapeutically effective amount of a derivative according to the present invention to said subject; to a method for sensitizing Gram-negative bacteria to an antibacterial agent by administering, simultaneously or sequentially in any order a therapeutically effective amount of said antibacterial agent and a derivative according to the present invention to said subject; to methods for developing novel antibiotics; for reducing the nephrotoxicity, for improving the pharmacokinetic properties of natural polymyxins and octapeptins; and for sensitizing clinically important bacteria to a host defence mechanism complement present in serum. Finally, the invention relates to a process for preparing such polymyxin derivatives.

The invention relates to genitourinary tract microorganism cultivation and detect method thereof, in particular to a genitourinary tract ureaplasma urealyticum (Uu) and mycoplasma hominis (Mh) culture medium and a detect method thereof. The culture medium comprises a fluid medium and a solid medium which are combined when the medium is used for culturing, separating and differentiating mycoplasma. The formulation comprises essential elements such as tryptic soy broth, yeast extract, urea, arginine, L-cysteine, phenol red, HEPES liquid, calf (fetal calf) serum, manganese sulfate, vancomycin, amphotericin B, trimethoprim, polymyxin, penicillin, and the like, and thereby the nutrition for mycoplasma growth and the antibiotic combination for inhibiting undesired microbe growth are optimized, no millipore filter is needed for sample inoculation and subcultivation, and the mycoplasma grows quickly. The fluid medium can maintain and prolong the logarithmic phase and indicate the growth of the mycoplasma, and the solid medium can used for observing Uu and Mh bacterial colonies, which has large background reflectance, thereby being easy for differentiation and significantly increasing the sensibility and the specificity of genitourinary tract mycoplasma detect.

The present invention discloses one kind of fertilizer plus farm chemical composition, which consists of ferrous sulfate 15-45 weight portions, ammonium molybdate 0.5-5 weight portions, magnesium sulfate 5-15 weight portions, composite amino acids 5-14 weight portions, zinc sulfate 1-10 weight portions, copper sulfate 0.5-5 weight portions and polymyxin 1-5 weight portions, and may include optional carrier, surfactant, dispersant and agricultural auxiliary agent. The fertilizer plus farm chemical composition has excellent preventing and controlling effect on the fungal, bacterial and physiological diseases of soybean and other plants.

The invention discloses an antibacterial drug composition for treating Gram-negative bacterial infections. The composition is prepared from TNP-2092 and a cell membrane penetrant, wherein the cell membrane penetrant is polymyxin B or polymyxin E. According to the antibacterial drug composition for treating Gram-negative bacterial infections, the Gram-negative bacterial infections are treated through the combined use of TNP-2092 and the cell membrane penetrant, the antibacterial activity of the composition is enhanced compared with the use of TNP-2092 or the cell membrane penetrant alone, and the composition has an antibacterial synergistic effect and can be used for treating the Gram-negative bacterial infections, including drug-resistance bacterial infections.

The invention provides an arenicola cristata antibacterial peptide NZ17074 derived peptide N6 and application thereof. An antibacterial peptide NZ17074 molecular structure is optimally designed to obtain a novel derived peptide N6, and the amino acid sequence of the novel derived peptide N6 is shown as SEQ ID NO: 1. The antibacterial peptide N6 provided by the invention has a very good sterilization effect on Gram-negative bacteria, and is used for remarkably reducing the haemolytic activity on mouse erythrocytes and the cytotoxicity on mouse original macrophages. A mouse toxemia model test result shows that the antibacterial peptide N6 also has an endoxemia resisting effect, and can be used for remarkably improving the survival rate of mice, and the effect of the antibacterial peptide N6 is better than that of an antibiotic polymyxin. The antibacterial peptide N6 has small molecular weight and is easily artificially synthesized; the antibacterial peptide N6 is a low-molecular-weight polypeptide with extremely good application value, can be used for preparing novel antibacterial and anti-infection medicines and the like, and has a good application prospect.

The invention belongs to the technical field of medicines, and in particular relates to a polymyxin E composition and a preparation method and application thereof. In the composition, total content of polymyxins E1, E2, E3, E1-1 and E1-7MOA is more than or equal to 80 percent, wherein the polymyxin E is preferably polymyxin E sulfate or polymyxin E sodium mesylate, and is particularly preferably polymyxin E sodium mesylate. The polymyxin E composition can be used for preparing medicines for treating Gram-negative bacterial infections.

The invention discloses a LAMP primer group, a kit and a detection method for rapid test of a bacterial polymyxin drug resistance gene mcr-1. The kit comprises a pair of outer primers, a pair of inner primers and a pair of loop primers. The six specific primers and a DNA polymerase with strand displacement activity are adopted for amplification of a DNA sample at 60-65 DEG C, and color changes in a reaction tube can be observed to judge whether amplification occurs or not after addition of a color developing agent. The LAMP primer group, the kit and the detection method have advantages of quickness, high efficiency, simplicity and convenience in operation, high specificity, high sensitivity, simplicity and convenience in detection, suitableness for onsite detection and the like and is suitable for popularization and application.

The present invention relates to a polymyxin synthetase isolated from Gram-positive Paenibacillus sp. and a gene cluster encoding the same, more precisely a polymyxin synthetase isolated from Paenibacillus polymyxa E681, ATCC21830 and F4 strains, a gene cluster encoding thereof and a preparation method of polymyxin or its derivatives using the gene cluster. The polymyxin synthetase of the present invention can be effectively used for the increase of productivity of polymyxin and the development of a novel antibiotic.

The invention discloses a culture medium and micro-culture method of cholera vibrio through this culture medium, wherein the culture medium comprises the following parts: peptone, beef grease, sodium chloride, agar, yeast leaching grease, anhydrous sodium sulfite, sodium citratej, dodecyl sodium sulfate, sucrose, phenol red, garamycin, polymyxin, potassium tellurite, benzalkonium bromide and distilled water; the method comprises het following steps: adding the mixed, melt and heat materials on the even pit of culture plate; putting the solidifying culture plate through plastic vacuum bag in the fridge; keeping under 2-6 deg.c; fetching the culture plate at the bacteria culture time; opening the package; seeding the detected sample in the pit; stewing for 9-18 h at 36+-1 deg.c; observing the result of culture plate; displaying yellow if there is growing bacteria; fitting for serum experiment directly.

Described are compounds of formula (I) for use in combination treatment with a second active agent, such as rifampicin, for example for treatment of a microbial infection. The compound of formula (I) is a polymyxin compound is:where the groups -A-, —R1, —R2, —R3, —R4, —R5, —R6, —R7, —R8, and —X— are described in detail within the description.

The antimicrobial hydrogel wound dressing is a swellable polymer gel made from about 7-9% (wt / vol) polyvinyl alcohol (PVA), preferably 8.9%, about 0.1% (wt / vol) polyvinyl pyrrolidone (PVP), and about 1-2% (wt / vol) agar, preferably 1%, the balance (about 90%) being distilled water, the foregoing contents being crosslinked by gamma radiation at a dose of about 30 kGy. Prior to crosslinking by gamma radiation, an effective amount of a pair of antibiotics is added to the gel at room temperature. The antibiotics include about 10,000 IU of polymyxin B sulfate, and about 5 mg neomycin per gram of gel. The polymyxin provides effective protection against various forms of gram negative microorganisms, and the neomycin is a broad spectrum antibiotic that provides protection against various forms of gram positive microorganisms. The hydrogel has sufficient mechanical strength for use as a wound dressing, and is capable of absorbing water up to 900% of its volume.

The present invention relates to a polymyxin synthetase isolated from Gram-positive Paenibacillus sp. and a gene cluster encoding the same, more precisely a polymyxin synthetase isolated from Paenibacillus polymyxa E681, a gene cluster encoding thereof and a preparation method of polymyxin or its derivatives using the gene cluster. The polymyxin synthetase of the present invention can be effectively used for the increase of productivity of polymyxin and the development of a novel antibiotic.

The invention discloses a new type of Neisseria gonorrhoeae medium, which is characterized in that the components include basal medium, sheep blood and bacteriostatic agent: ①Basic medium: 1.0-2.0 parts of peptone, 0.05-0.15 parts of cornstarch, K2HPO40. 2-0.8 parts, KH2PO4 0.05-0.2 parts, NaCl 0.25-1.0 parts, agar 1-3 parts; ② sheep blood 3-10 parts; ③ bacteriostatic agent: vancomycin 0.003-0.005 parts or lincomycin 0.003-0.01 parts, polymyxin 0.0075-0.015 parts, nystatin 0.417-0.667 parts and trimethoprim 0.005-0.01 parts. The culture medium is suitable for grassroots medical institutions, has excellent performance, and has a simple and reasonable preparation method, which not only overcomes the shortcomings in the prior art, but also ensures the effect of separation and culture.

The invention relates to a sorbent for removing endotoxins from a biological liquid, comprising a water-insoluble, porous substrate having a neutral, hydrophobic surface, wherein the surface of the substrate has an adsorbent coating made of polymyxin and albumin, wherein polymyxin and albumin are bonded to the surface of the substrate in a noncovalent manner. The invention further relates to a method for producing such a sorbent. The sorbent is used in extracorporeal blood purification, in particular in order to treat individuals who have sepsis.

The present invention relates to a polymyxin synthetase isolated from Gram-positive Paenibacillus sp. and a gene cluster encoding the same, more precisely a polymyxin synthetase isolated from Paenibacillus polymyxa E681, a gene cluster encoding thereof and a preparation method of polymyxin or its derivatives using the gene cluster. The polymyxin synthetase of the present invention can be effectively used for the increase of productivity of polymyxin and the development of a novel antibiotic.

The invention discloses a method for synthesizing polymyxin E through a fermentation method, which comprises: inoculating bacillus polymyxa AS1.541 into culture medium to culture and is characterized in that the concentration of the organic nitrogen source which is used in the culture medium is 4%-10%. The highest fermentation unit of the polymyxin E which is synthesized through fermenting by the method of the invention can reach 22143.7U / ml.