167results about How to "Guaranteed Quality Control" patented technology

The invention relates to a method for separating and extracting stem cells from a placenta, umbilical cord or adipose tissue, which comprises the following process steps: firstly mixing the placenta, umbilical cord or adipose tissue and a cell maintenance fluid according to the proportion by weight of 2.5-4:1, putting the mixture into a tissue crushing barrel, adding collagenase after crushing, uniformly mixing, hatching at the temperature of 37 DEG C, filtering, adding a precipitator, sucking a supernatant fluid after settling, centrifuging, removing the supernatant fluid, adding concentrated cells into a liquid of diatrizoate sodium-ficoll 400#, then centrifuging, collecting 10-15 ml of intermediate cell layer, washing with the cell maintenance fluid, counting the collected cells, and providing the cells for clinical use when the cell survival ratio is more than or equal to 95 percent. The invention not only realizes the separation and the extraction of all stem cells from the placenta, umbilical cord or adipose tissue, but also realizes industrialized production, and enables doctors to conveniently, safely and canonically obtain the adult stem cells in clinic and use the adult stem cells for treating the diseases of patients as using medicaments, thereby solving the bottleneck problem of difficult obtainment of adult stem cells in clinic and popularizing a cell treatment technology.

The invention relates to the technical field of detection, and discloses detecting device and method. The detecting device comprises an image acquiring module and an image processing module, wherein the image acquiring module acquires image information of a liquid crystal display panel to be detected, and the image processing module is in signal connection with the image acquiring module. The image processing module compares received image information with a defect database and judges defect level of the liquid crystal display panel to be detected. During use of the detecting device, the image acquiring module can acquire image information of any position of the liquid crystal display panel to be detected, the image processing module compares received the image information acquired by the image acquiring module with the defect database and judges the defect level of the liquid crystal display panel to be detected. Accordingly, by the detecting device, defect identification rate and detection efficiency of the liquid crystal display panel are improved.

The invention discloses a sectional cutting and overall craning method for a supporting beam in deep foundation pit multilayered reinforced concrete. The method comprises the steps of internal force measurement, cutting and dismantling, transferring in the foundation pit, entirely craning of the supporting beam, off-site crushing of the supporting beam and the like. The method is to construct on an underground horizontal thin slab structure without special requirement on the construction platform. A steel pipe supporting system of the underground thin slab structure is not required to be reinforced, but holes and expansion joint positions are reserved for the underground horizontal thin slab to be locally reinforced. When the supporting beam is cut off and dismantled, the steel pipe stand is not required for the lower part. The single input of the steel pipe stand material is greatly reduced on the site, and the steel pipe stand can be dismantled in advance to improve the turnover rate of the steel pipe stand.

The invention relates to a control method for a shield tunnel axis having an underground structure on an adjacent side, comprising the following steps: using a three-dimensional finite element model to numerically stimulate the shield, setting a range L1 in which the underground structure starts influencing on near pass-through of the shield and a length L2 of the segment in which priority needs to be given to the tunnel axis; regarding a segment that is 20 meters away from the focused protection segment L2 as an experimental advancing section of the shield; installing a PVC pipe having the diameter of d1 and an opening on the side thereof in the soils between the shield tunnel and the adjacent underground structure in the range L2 determined by step (1), increasing channels from which pore water is drained and decreasing aggregation of pressure of the pore water; accurately measuring indicators that control the quality of the tunnel axis; and monitoring and real-time regulating. The invention solves the problem that the control of the shield tunnel axis is influenced by the existence of the underground structure, guarantees normal usage of the underground structure and quality control of the shield tunnel axis, and is suitable for similar working conditions.

The invention relates to a method for preparing liquid smoking spice for meat from pecan shells. In the method, the byproduct shells of wild pecans in China are used as the materials. The method comprises the following steps: selecting the material, pulverizing, carrying out dry distillation, filtering, fractionating, and aging at variant temperatures to obtain the stable finished product. The liquid smoking spice can be used for meat. The content of smoking characteristic flavoring substance 2,6-dimethoxyphenol is up to 8.0-18.0 (mg / mL), and the content of polycyclic aromatic hydrocarbon 3,4-benzopyrene is controlled within 2.0 mu g / kg, thereby enriching the diversity of smoking spice products and fully utilizing the pecan shells. By combining variant temperature aging with micro-filtering to remove precipitated tar, the aging time is shorted to 4 days, which is shortened by more than half as compared with the current like products, thereby greatly enhancing the production efficiency.

The invention relates to the field of ultrasonic medical images, and provides an artificial intelligence ultrasonic auxiliary system and application thereof. The system is mainly applied to an ultrasonic machine, an image system and telemedicine. The artificial intelligence ultrasonic auxiliary system comprises an image collection module. The system comprises an image transmission module, an imageframing module, an automatic classification module, an automatic film selection module, a teaching auxiliary module, a film reading prompt module and a quality control analysis module, realizes automatic classification of cases, automatically selects a standard section for negative cases, and provides functions of automatic film reading prompt, teaching assistance, quality control analysis and the like for suspected cases. After an ultrasonic auxiliary system based on artificial intelligence is implanted into an ultrasonic machine, an image information system and a remote medical system, doctors with low annual resources can quickly start, and the defect of basic medical resources is overcome.

Optical film visual intelligent detection equipment comprises a feeding unit, a detection unit and a collection unit; the feeding unit comprises a feeding module and a protection film uncovering mechanism, the protection film uncovering mechanism comprises an adhesive tape feeding module, an adhesive tape pressing and attaching module and a protection film uncovering module; the detection unit comprises a lighting module, a camera shooting module and an upper computer; the collection unit comprises a protection film attaching mechanism, a defective-product marking mechanism and a collection module, and the protection film attaching mechanism comprises a protection film feeding module and a protection film pressing and attaching module. The optical film visual intelligent detection equipment is mainly used for full-automatically detecting optical film products in a visual intelligent detection way to further screen out defected optical film products, and has the advantages of high detection efficiency, high detection reliability; meanwhile, because of relatively reasonable structure design, the advantages of compact structure, small occupied area and relatively low cost are achieved; above all, not only is the defect identification rate of the equipment to the optical film products high, but also false detection cannot occur, so that the quality control of the products is ensured.

The invention discloses a flexible production line for a spatial three-dimensional reinforcing mesh, which comprises a stirrup cloth production line, a bending production line, a main reinforcement cloth production line, and a gantry production line. The present invention also discloses a flexible production process for the spatial three-dimensional reinforcing mesh, which includes the following steps: S1, preparing stirrups and completing plane distribution; S2, pulling the stirrups onto a bending platform; S3, horizontally moving a guide beam in the same longitudinal direction so that the stirrups are planarly bent according to a set bending angle; S4, sequentially distributing main reinforcement; S5: welding and binding to form a mesh; S6, successively performing operations from an outside three-dimensional bending mechanisms to bend the mesh twice from the two sides of the production line to its centerline to achieve three-dimensional bending forming of the mesh; and S7, after themesh is formed, lifting the mesh out of the production line. The production line and the production process of the invention can improve the industrialization, mechanization, automation, and assemblylevel of the bridge tower spatial three-dimensional reinforcing mesh construction .

The invention relates to a primer pair for colorectal-cancer related gene Septin9 methylation detection. The primer pair comprises a Septin9 forward primer, a Septin9 reverse primer, a Septin9 detection probe, a beta-Actin forward primer, a beta-Actin reverse primer and a beta-Actin detection probe. The invention relates to a kit for colorectal-cancer related gene Septin9 methylation detection. The kit comprises PCR reaction liquid comprising the primers and the probes, and the PCR reaction liquid comprises the forward primers, the reverse primers, the detection probes, 10*PCR buffer, dNTP, nuclease-free water and Ex Taq enzyme. The invention also relates to a method for colorectal-cancer related gene Septin9 methylation detection. The kit and the detection method have the advantages of being accurate in detection result, high in detection flux, good in specificity and sensitivity, short in detection time, convenient to use and capable of effectively meeting the clinical requirements.

The invention provides a positive ion lipid nanometer particle / DNA compound and a preparation technology thereof. The preparation technology comprises the following steps that (1) a positive ion lipidmaterial is dissolved in absolute ethyl alcohol through heating; (2) an ethyl alcohol solution prepared in the step (1) is added to an aqueous phase solution dropwise, and positive ion lipid nanometer particles are formed through self-assembling; (3) residue ethyl alcohol in the positive ion lipid nanometer particles of the step (2) is removed; (4) filtering is carried out; (5) a DNA solution isprepared; (6) the prepared positive ion lipid nanometer particles in the step (4) and the DNA solution prepared in the step (5) are mixed according to a certain weight ratio to form the positive ion lipid nanometer particle / DNA compound; and (7) filtering is carried out. According to the preparation technology of the positive ion lipid nanometer particle / DNA compound, the operation of the preparation method is easy and rapid, the particle diameter of the prepared positive ion lipid nanometer particle / DNA compound is 50-150 nm, PDI<0.3, the positive ion lipid nanometer particle / DNA compound isdistributed in a mono-dispersion mode, the structure is stable, the compound is subjected to filtering sterilization at a terminal, and the security of the compound in clinic application of preparation of tumor curing medicine is effectively ensured.

The invention discloses a preparation method of a burkholderia gladioli molecular standard sample. The preparation method comprises the step of carrying out freeze drying on extracted genome nucleic acid of burkholderia gladioli, wherein the freeze drying conditions are as follows: a freeze dryer is started, and a cooling trap is started when temperature of a drying chamber is lowered to -35 DEG C; when the temperature of the cooling trap is lowered to -40 DEG C, a nucleic acid sample prefrozen for 2 hours at the temperature of -80 DEG C is put into the drying chamber and then vacuumized; when vacuum degree is reduced to 0.5Torr, freezing is finished; and the sample is dried at the temperature of 15 DEG C, the sample is taken out when the vacuum degree is reduced to 0.1Torr, a penicillin bottle is tightened to obtain a burkholderia gladioli genome nucleic acid standard sample, and the sample is kept in a dark place and stored at the temperature of 20 DEG C. The molecular standard sample disclosed by the invention has good physical and chemical properties and can be detected by adopting a specific primer through PCR process, and sensitivity reaches 10<-4>. Detection test shows that the standard sample has good DNA integrity, uniformity and stability, can be applied to import and export detection as a positive reference material, and accuracy of a detection result is improved.

The invention relates to a primer pair for methylation detection of a lung cancer related gene SHOX2. The primer pair comprises an SHOX2 forward primer, an SHOX2 reverse primer, an SHOX2 detection probe, a beta-Actin forward primer, a beta-Actin reverse primer and a beta-Actin detection probe. The invention relates to a kit for the methylation detection of the lung cancer related gene SHOX2. The kit comprises PCR reaction liquid containing the primers and probes above, the PCR reaction liquid comprises the forward primers, the reverse primers, the detection probes, 10*PCR buffer, dNTP, nuclease-free water and Ex Taq enzymes. The invention further relates to a method for the methylation detection of the lung cancer related gene SHOX2. The kit and the detection method thereof have the advantages of being accurate in detection result, high in detection flux, good in specificity and sensitivity, short in detection time, convenient to use and capable of effectively meeting clinical requirements.

The invention discloses an HPLC wavelength switching method for simultaneously determining the gallic acid content and the ziyuglycoside I content in sanguisorba officinalis l or a sanguisorba officinalis l preparation. The HPLC wavelength switching method comprises: (1) establishing a standard curve of gallic acid and ziyuglycoside I: a, preparating a reference substance solution, and b, determining the reference substance solution; and (2) determining the gallic acid content and the ziyuglycoside I content in a sample to be detected: c, preparing a sample solution, and d, determining the sample solution. According to the present invention, the HPLC wavelength switching method for simultaneously determining the gallic acid content and the ziyuglycoside I content in the sanguisorba officinalis l or the sanguisorba officinalis l preparation is successfully established, the gallic acid content and the ziyuglycoside I content can be simply, rapidly, accurately and simultaneously determined, the active component content in the sanguisorba officinalis l or the sanguisorba officinalis l preparation can be completely monitored, the intrinsic quality of the drug can be accurately reflected, and the effective assurance is provided for the quality control of the sanguisorba officinalis l or the sanguisorba officinalis l preparation.

The invention provides an automatic pole piece compaction density control system and an automatic pole piece compaction density control method. A grinding production line is used for passing of a coated battery pole piece; the production of the pole piece is controlled by a grinding production line control system; the surface density of a pole piece is detected through a double-faced penetration type beta sensor; the thickness of the pole piece is detected through a double-faced laser sensor; the detected data is transmitted to the grinding production line control system through a data transmission network; the pole piece is grinded through a grinding and rolling roller; a servo control system is mounted on the grinding and rolling roller to control a rolling roller gap; a position detecting sensor is mounted on the grinding and rolling roller to detect the practical rolling roller gap. The automatic pole piece compaction density control system is simple in structure and reasonable in design, manual interferences and adjustment to the pole piece rolling degree are reduced, and the automation adjustment is realized; lag phenomena to the adjustment to the pole piece thickness and the compactness density in a production process are reduced. In a pole piece grinding process, the compactness density is automatically and stably controlled, so that the equipment is relatively intelligent. The invention provides a novel pole piece grinding method which can be used for broadening a control mode of the polar piece grinding production.

The invention discloses a method for protecting and purifying heparin sodium under a resin adsorption state. The method takes a heparin sodium crude product as a raw material, after dissolving, resinadsorption is carried out, under the resin adsorption state, mixed salt solution is employed to remove nucleic acid, salt solution is carried out to remove binding protein, a salt and ethanol mixed solution is employed for performing ultrasonic removing of an ethanol-soluble object, salt is employed for gradient elution and desorption, a methanol and ethanol mixed solution is used for ultrasonic decolouring, absolute ethyl alcohol is used for ultrasonic dehydrating to remove methanol, a sediment is subjected to dissolving and ultrafiltration to remove salt and alcohol, and a fine product heparin sodium is prepared through freeze-drying. The method abandons a traditional heparin sodium chemical oxidation impurity-removing purifying method, the prepared heparin sodium has the advantages of complete molecular structure, less fracture, and good stability, the unit titer is more than 180 IU / mg, and the titer yield is 98-99.5%. The heparin sodium as a raw material is in favor of stability ofa heparin preparation, and is in favor of controlling important quality indexes such as weight average molecular weight and molecular weight distribution of the low molecular heparin preparations such as enoxaparin sodium.

The invention relates to a construction method for a post-injection cement mortar masonry brickwork, and relates to a masonry construction method for masonry in building engineering. The construction method includes arranging a groove on at least one surface of each brickwork component; building the brickwork components under a mortar-free condition in a self-stabilizing manner; arranging mortar injecting holes used for filling mortar at corresponding positions of masonry which is built in a self-stabilizing manner; injecting the mortar in the grooves among the brickwork components via the mortar injecting holes; cleaning spilt mortar; and plastering and whitewashing the masonry according to the traditional mode and completing a project. The construction method is practical and reliable, materials can be obtained conveniently, manufacture is simple, service is convenient, and effects are good. Besides, problems of large quantities of occupied workers, unprofessional working procedure division, low work efficiency and the like in an existing masonry construction process are solved. In addition, workload for constructing the traditional masonry is divided into three blocks including that the masonry components are effectively manufactured in a factory in a standard manner, continuous building of the masonry components is unaffected in a field, and the cement mortar is injected in a later stage. Division of labor based on specialization is clear, the quantity of each working procedure can be assuredly controlled, and work efficiency is greatly improved.

The invention belongs to the technical field of molecular biology and relates to a detection product for genomic methylation, in particular to a primer probe combination for methylation detection of an RASSF1A gene and a P16 gene and application of the primer probe combination. The primer probe combination for the methylation detection of the RASSF1A gene and the P16 gene comprises detection primer probes; and the detection primer probes comprise an RASSF1A gene methylation detection specific primer pair, an RASSF1A gene specific probe, a P16 gene methylation detection specific primer pair anda P16 gene specific probe. According to the primer probe combination, methylation sites of the RASSF1A gene and the P16 gene are accurately detected, detection precision of ctDNA is remarkably improved, and absolute quantification is realized; and in addition, the primer probe combination has high specificity and accuracy, and is suitable for early screening of liver cancer.

The invention discloses a method for construction of a continuous grid frame 0# section or a linear section through an assembly-type bracket corbel construction device, and particularly relates to a method for construction of a continuous beam linear segment without a floor stand. The method comprises the following steps that (1) a horizontal transverse beam, a vertical supporting mechanism, a slant supporting mechanism, a tie bar, a shear-resisting plug, an embedded steel tube and an embedded steel box are manufactured; (2) the embedded steel tube and the embedded steel box are installed; (3) a corbel tripod is formed by the horizontal transverse beam, the vertical supporting mechanism and the slant supporting mechanism; (4) when the corbel tripod is installed in place, stress is borne by the shearing-resisting plug, the tie bar penetrates through the embedded steel tube and is used for tensioning the horizontal transverse beam and the two ends of the tie bar are fixed; (5) after a bracket is installed, following construction of a bottom die platform and beams is conducted; (6) connection bolts of the tripod are detached, the tripod is put down in a hanging mode and is detached into independent rods which are not assembled, then, the shearing-resistant plug and finish-rolled threaded steel pull bars are detached, and all components are transferred to the next construction point for reassembly or are transferred to a stock for storage.

The invention belongs to the technical field of medicines, and in particular relates to a polymyxin E composition and a preparation method and application thereof. In the composition, total content of polymyxins E1, E2, E3, E1-1 and E1-7MOA is more than or equal to 80 percent, wherein the polymyxin E is preferably polymyxin E sulfate or polymyxin E sodium mesylate, and is particularly preferably polymyxin E sodium mesylate. The polymyxin E composition can be used for preparing medicines for treating Gram-negative bacterial infections.

The invention relates to a solvent desorption device. The solvent desorption device comprises an adsorption pipe. The solvent desorption device is characterized by also comprising an adsorption pipe connector and a pump, wherein the adsorption pipe connector is used for connecting the adsorption pipe, which adsorbs to-be-detected samples, into a desorption flow channel; the pump enables a desorption liquid to circulate in the desorption flow channel so as to desorb the samples in the adsorption pipe and make the samples in a balanced state. The invention also provides a solvent desorption method. The solvent desorption device and method have the advantages of time saving effect, high desorption efficiency and high desorption repetition rate.

The invention discloses a HPLC method for simultaneously determining the gallic acid content and the (+)-catechin content in a radix sanguisorbae preparation.The gallic acid content and the (+)-catechin content can be determined simply, conveniently, accurately and simultaneously, wherein the detection limit of gallic acid reaches 0.62 ng, the average recovery rate of the gallic acid reaches 98.99%, the detection limit of (+)-catechin reaches 12.20 ng, and the average recovery rate of the (+)-catechin reaches 101.09%.Therefore, the method has the advantages that the active ingredient content in the radix sanguisorbae preparation can be more comprehensively monitored, the inherent quality of medicine is more truly reflected, and an effective guarantee is supplied to quality control over the radix sanguisorbae preparation.

The invention discloses a method for measuring surface defects based on a spectral confocal sensor. A plane reflector is installed on an angular displacement table, the distance between a spectral sensor and a to-be-measured workpiece is adjusted, the acquisition of spectral peak intensity data at a set inclination angle is finished, the angular displacement table is adjusted to be at different inclination angles, until the acquisition of the spectral peak intensity data at all specified inclination angles is finished; a "steepness-distance-spectral peak intensity" characteristic curve is established; scanning measurement is performed on the to-be-measured workpiece, spectral peak intensity data of return light on the surface of the workpiece is acquired, and a reflectivity matrix of the surface of the to-be-measured workpiece is calculated; and a reflectivity threshold value is set, and a reflectivity sudden change region is identified by setting a reflectivity threshold value region,so that a defect region and a normal region are distinguished, and defect positioning and contour extraction are completed. A mathematical model between the spectral peak intensity and the surface reflectivity is built by an experiment method, and the surface defects are positioned by the reflectivity, so that a novel method is provided for the detection of the surface defects of optical elements.

The invention belongs to the technical field of medicine analysis, and particularly relates to a high performance liquid chromatography for determinating the taurine content of taurine granules or taurine eye drops based on a differential detector. According to the high performance liquid chromatography for determinating the taurine content based on the differential detector, high performance liquid chromatography is combined with a differential refraction detector to establish a high performance liquid chromatography-differential detection method for directly determining the taurine content;with an NH2 column as a separation column, detection is performed by using a differential refraction display; in the whole detection process, use of a carrier gas is not required and derivatization oftaurine is not required; in addition, no intermediate reaction process exists, the linear range is wide, and the taurine content can be directly determined easily, conveniently, quickly and accurately; by the high performance liquid chromatography, determination of the taurine content of the taurine granules and the taurine eye drops can be achieved.

The invention relates to a porcine circovirus type 2 double antibody sandwich ELISA reagent kit and a detection method. The reagent kit includes an enzyme label plate coated with a monoclonal antibodyagainst a porcine circovirus Cap protein, a blocking buffer, a primary antibody, an enzyme-labeled secondary antibody, concentrated washing liquid, a colored solution, a stopping solution, a standard, a positive contrast, and a negative contrast. The porcine circovirus type 2 double antibody sandwich ELISA has the advantages of short detection cycle, simple operation, high sensitivity, strong specificity and simple equipment requirements and can perform qualitative, semi qualitative and quantitative analysis. Moreover, the reagent kit can achieve rapid detection of large quantities of samples. The invention also provides an application of the reagent kit in detecting an antigen of a porcine circovirus type 2 inactivated vaccine.

The invention discloses a novel laser engraving device which comprises a horizontal worktable, wherein a rotating cavity is formed in the horizontal worktable; supporting rods are symmetrically arranged at the left and right ends of the upper end surface of a final assembly worktable, and the upper ends are connected between the supporting rods through a push rod in a hydraulic cavity through a fixed block; a lifting cutting device is mounted on the rod body of the push rod in a sleeving manner; a plurality of screw-up mechanisms and four same screw-up screws matched with the screw-up mechanisms in use are uniformly distributed in the middle position of the upper end surface of the worktable. During work, blank waste caused by displacement and processing failure when workpieces are processed by means of a clamping structure formed by a pressing spacer and a round supporting plate is avoided. A lifting cutting device shifts left and right through a hydraulic pump, so that the working point can be determined more accurately, the processing precision is improved. A rotating gear A and a gear slot are matched, so that the friction coefficient can be reduced.

The invention discloses an analysis method for impurity content of exenatide, and belongs to the technical field of medicine analysis. According to the method provided by the invention, the exenatideand three specific impurities are digested by using trypsin, after digestion, a sample is separately separated by using two chromatographic processes, impurity [D-His<1>]-exenatide, impurity [Double Pro<38>]-exenatide and impurity [Des-Gly<2>]-exenatide contents in the exenatide are computed and measured by using an external standard method. The method provided by the invention is convenient, efficient and high in accuracy, and can effectively separate impurities and accurately quantify the same.

The invention discloses a novel gas turbine low-pressure compressor rotor structure and an assembling method. The rotor structure comprises one to six stages of working impellers, and two adjacent stages of working impellers are connected through a passage ring assembly and a distance ring assembly; the first-stage working impeller is connected with a front sealing labyrinth disc, and the sixth-stage working impeller is connected with a rear sealing labyrinth disc; the distance ring assembly and the working impeller are connected through a bolt, and a locking nut is screwed in the bolt. The passage ring assembly is connected with the working impeller through a pin, a pin hole is formed between the passage ring assembly and the working impeller, and swing gaps are formed between the pin and the pin hole and between the passage ring assembly and the working impeller. The assembling method comprises the following steps of aligning all the parts in places; and pre-tightening the locking nut on the bolt, unscrewing for two circles after 20-30 minutes, and finally and tightly screwing in. According to the present invention, centering and torque transmission between parts are guaranteed, and good centering and reliable force transmission can be achieved.

The invention discloses a preparation method for a standard sample of an infectious hematopoietic organ necrosis virus molecule. The preparation method is characterized by including following steps: extracting an infectious hematopoietic organ necrosis virus RNA (ribonucleic acid); performing RT-PCR (reverse transcription-polymerase chain reaction); purifying target genes; performing connection transformation of target segments; recycling plasmid; performing in vitro transcription; performing RNA purification after transcription; and performing phenol chloroform extraction. The preparation method is time-saving and labor-saving in a preparation process, the standard sample of prepared infectious hematopoietic organ necrosis virus ribonucleic acid is high in stability and fine in uniformity, standard samples can be provided for infectious hematopoietic organ necrosis virus detection researches, medicine researches, application researches and the like, so that comparison of different laboratory results is realized, and quality control of laboratories is guaranteed.