35 results about "Hematopoietic organ" patented technology

The invention discloses a method for preparing a rainbow trout IHN (Infectious Haematopoietic Necrosis) inactivated vaccine, which comprises the following steps: carrying out grinding, filtering and poison dipping processing on pancreas and livers of juvenile fish which is attacked, but is still alive, inoculating rainbow trout gonad (RTG) cells, carrying out blind passaging at 14 DEG C, keeping for 5 days when carrying out blind passaging on the tenth generation, and collecting cell poisonous fluid; inoculating chinook salmon embryonic (CHSE) cells, carrying out passaging at 14 DEG C, and raising the culture temperature by 1 DEG C when passaging for 5 generations each time until the culture temperature is raised to 20 DEG C; and adopting epithelioma papulosum cyprini (EPC) cells to continuously carry out passaging under the condition of 20 DEG C, passaging to the twelfth generation to obtain high-titer virus solution and carrying out inactivation to obtain the rainbow trout IHN inactivated vaccine. According to the preparation method, RTG-2, CHSE-214 and EPC cells are utilized to alternately culture rainbow trout IHN viruses at a specific environment temperature so as to obtain a high-titer IHNV (Infectious Hematopoietic Necrosis Virus) antigen and produce the inactivated vaccine; and the technical difficult problem that the high-titer IHNV antigen cannot be obtained through single cells is solved.

An inducing agent or enhancing agent, for the expression of HM1.24 antigen in hematopoietic tumor cells, comprising interferon α, interferon γ, or the IRF-2 protein as an active ingredient, as well as an anti-tumor agent for hematopoietic tumors which comprises a combination of said inducing agent or enhancing agent and an antibody against HM1.24.

The invention discloses an infectious hematopoietic organ necrosis vaccine and a method for amplifying a virus of the infectious hematopoietic organ necrosis vaccine on muscle cells of Pimephales promelas. The invention provides the method for proliferating infectious hematopoietic organ necrosis virus in vitro. The method comprises the following steps of: inoculating the infectious hematopoietic organ necrosis virus to muscle cells of Pimephales promelas according to the MOI value of 0.001, culturing, and collecting supernate, so as to proliferate the infectious hematopoietic organ necrosis virus on the muscle cells of Pimephales promelas. According to the present invention, the IHNV is inoculated to the FHM cell at the concentration of MOI = 0.001, the required virus collection time is short, and the virus titer is high and stable. According to the proliferation scheme, viruses are amplified on a large scale, the inactivated vaccine is prepared from BPL and formaldehyde, the result shows that the immune effect of the vaccine on rainbow trout is good, and the relative immune protection efficiency can reach 80% or above.

The invention discloses RT-LAMP detection primer pairs, a detection kit and a detection method for infectious haematopoietic necrosis virus (IHNV). The detection primer pairs comprises a pair of outer primers, a pair of inner primers and a pair of loop primers; the detection kit comprises primer liquid, reaction liquid, DNA polymerase, reverse transcriptase and control; and the detection kit also can contain a color developing agent. The detection method comprises the following steps: extracting to-be-detected virus RNA, and amplifying a sample RNA template at the temperature of 63-65 DEG C by utilizing reverse transcription activity of reverse transcriptase and adopting six specific primers and one DNA polymerase with strain displacement activity, wherein the pg grade of pure virus RNA can be detected; and identifying by adding SYBR Green I ESE-Quant-tube Scanner instrument detection, or utilizing a turbidity meter for observing change of turbidity of sediments in a reaction tube, so as to judge whether to carry out amplification or not and determine whether the to-be-detected sample contains IHNV RNA or not. The RT-LAMP detection primer pairs, the detection kit and the detection method for the IHNV have the advantages of quickness, high efficiency, easy operation, high specificity, high sensitivity, easy identification and applicability to field detection and are applicable to popularization and application.

The invention discloses a preparation method for a standard sample of an infectious hematopoietic organ necrosis virus molecule. The preparation method is characterized by including following steps: extracting an infectious hematopoietic organ necrosis virus RNA (ribonucleic acid); performing RT-PCR (reverse transcription-polymerase chain reaction); purifying target genes; performing connection transformation of target segments; recycling plasmid; performing in vitro transcription; performing RNA purification after transcription; and performing phenol chloroform extraction. The preparation method is time-saving and labor-saving in a preparation process, the standard sample of prepared infectious hematopoietic organ necrosis virus ribonucleic acid is high in stability and fine in uniformity, standard samples can be provided for infectious hematopoietic organ necrosis virus detection researches, medicine researches, application researches and the like, so that comparison of different laboratory results is realized, and quality control of laboratories is guaranteed.

The invention discloses an infectious hematopoietic necrosis vaccine and a method for amplifying infectious hematopoietic necrosis virus (IHNV) on epithelioma papulosum cyprini (EPC). The invention provides a method for preparing an infectious hematopoietic necrosis vaccine, and the method comprises the following steps: 1) inoculating IHNV to EPC according to an MOI value of 0.0001, carrying out culturing, and collecting supernatant, thereby obtaining proliferated IHNV; and 2) preparing the infectious hematopoietic necrosis vaccine by using the proliferated IHNV. Experiments in the invention prove that when the IHNV is inoculated to the EPC with the concentration of MOI = 0.0001, the required virus harvest time is short, and the virus titer is high and stable. The IHNV is amplified on a large scale based on the proliferation scheme, and an inactivated vaccine is prepared from BPL and formaldehyde. Results show that the vaccine has a good immune effect on Oncorhynchus mykiss, and the relative immune protection efficiency was up to 84%.

The invention provides an inactivated vaccine for an epizootic haematopoietic necrosis disease in carassius auratus and a preparation method of the inactivated vaccine for the epizootic haematopoieticnecrosis disease in the carassius auratus. The vaccine comprises spinal cord tissue cell lines of carassius auratus gibelio and cyprinidherpesvirus II, and a preservation number of the spinal cord tissue cell lines of the carassius auratus gibelio is CCTCC NO: C2018211. The preparation method comprises the following steps: performing CyHV-2 virus amplification on the cultured spinal cord tissue cell lines of the carassius auratus gibelio to obtain CyHV-2 virus solutions; and performing inactivation treatment on the CyHV-2 virus solutions. The vaccine prepared by adopting the method disclosedby the invention has a good immunoprotection effect, can be applied to preventive immunity for the epizootic haematopoietic necrosis disease in the carassius auratus, and can increase survival ratiosof raised carassius auratus and the raising efficiency of the carassius auratus.

The invention discloses a high-efficient PCR (polymerase chain reaction) detection method of infectious hematopoietic necrosis virus and relates to a detection method of the infectious hematopoietic necrosis virus. The invention aims at the problem that the existing PCR method for inspecting the infectious hematopoietic necrosis virus is not high in sensitivity. The method comprises the following steps: 1) designing specific primers IHNV-Lf and IHNV-Lr; 2) getting a tissue filtrate after treatment of a detected pathological material; 3) preparing RNA (ribonucleic acid) of the IHNV (infectious hematopoietic necrosis virus); 4) performing PCR amplification to get an amplified product; and 5) observing the PCR amplified product and determining a result to end the detection. The detection method disclosed by the invention has great specificity, and the cross reaction with VHSV (viral hemorrhagic septicemia virus) does not exist; and according to the detection method disclosed by the invention, a polymerase protein is taken as a detection target, the specific primers IHNV-Lf and IHNV-Lr are utilized for detection, the operation is simple, the accuracy is high, the detection method is sensitive, the sensitivity under the condition of low concentration is great, the detection cost is relatively lower, and the detection method is more time-saving in comparison with an existing nested PCR (two-round PCR).

The invention relates to a method for detecting fish contagious hemopoietic organ necrosis virus based on RPA and belongs to the technical field of biodetection. The method comprises the following steps: determining RPA primers according to a virus G protein conserved sequence, an upstream primer: 5'-GATGAGTGGGAGGGCTTTCACGGATTGC-3' and a downstream primer: 5'-GCACGCGGCAACAGCAAGGAGGAGAACAAGG-3'; extracting virus RNA from an RNA extraction kit box, preparing cDNA from virus RNA through an inverse transcription kit and freezing the cDNA for later use; detecting impurity and content of cDNA undera nucleic acid protein analyzer according to OD260/OD280 and concentration value; preparing an RPA reaction system; and identifying an RPA product. The method has the beneficial effects that the specific primers are designed according to a gene G of an IHNV conserved sequence, and the detection method is simpler and more efficient, high in specificity and high in sensitivity, is not dependent on ahigh end instrument, is suitable for field rapid detection, and can control burst of fish epidemic diseases.

A medicament for improving prognostic survival in therapy of malignant tumor is provided that may improve prognostic survival in DIC patients where the basal disease is malignant tumor, especially malignant tumor in hematopoietic organs. The medicament according to the invention comprises as a main active ingredient Activated Protein C, which is obtained from plasma or prepared by using the genetic recombination technique, and efficiently prolongs life-span of DIC patients where the basal disease is malignant tumor, especially malignant tumor in hematopoietic organs. In particular, the medicament may reduce adverse side effects of chemotherapeutics in chemotherapy of malignant tumor to enhance efficacy of said therapy and improve prognostic survival of patients suffering from malignant tumor.

The present invention discloses a set of reagents for detection of infectious pancreatic necrosis virus. The kit for detecting infectious pancreatic necrosis virus disclosed in the invention consistsof six single-stranded DNAs named B2-F3, B2-B3, B2-FIP, B2-BIP, B2-LooF and B2-looB; B2-F3, B2-B3, B2-FIP, B2-BIP, B2-LooF and B2-looB are respectively SEQ ID No. in the sequence listing. Single-stranded DNA as shown in 1-6. The experiment proves that the kit of the present invention has good specificity for detecting infectious pancreatic necrosis virus, and does not cross-react with infectious hematopoietic organ necrosis virus (IHNV) and viral hemorrhagic septicavirus (VHSV); sensitivity is high, sensitivity is 0. .0008fg / 25[mu]L reaction system; detection is simple and time-saving.