35 results about "Hematopoietic organ" patented technology

The invention discloses a method for preparing a rainbow trout IHN (Infectious Haematopoietic Necrosis) inactivated vaccine, which comprises the following steps: carrying out grinding, filtering and poison dipping processing on pancreas and livers of juvenile fish which is attacked, but is still alive, inoculating rainbow trout gonad (RTG) cells, carrying out blind passaging at 14 DEG C, keeping for 5 days when carrying out blind passaging on the tenth generation, and collecting cell poisonous fluid; inoculating chinook salmon embryonic (CHSE) cells, carrying out passaging at 14 DEG C, and raising the culture temperature by 1 DEG C when passaging for 5 generations each time until the culture temperature is raised to 20 DEG C; and adopting epithelioma papulosum cyprini (EPC) cells to continuously carry out passaging under the condition of 20 DEG C, passaging to the twelfth generation to obtain high-titer virus solution and carrying out inactivation to obtain the rainbow trout IHN inactivated vaccine. According to the preparation method, RTG-2, CHSE-214 and EPC cells are utilized to alternately culture rainbow trout IHN viruses at a specific environment temperature so as to obtain a high-titer IHNV (Infectious Hematopoietic Necrosis Virus) antigen and produce the inactivated vaccine; and the technical difficult problem that the high-titer IHNV antigen cannot be obtained through single cells is solved.

The invention discloses an application of IL-2 protein to preparation of an animal vaccine adjuvant. The amino acid sequence of the IL-2 protein is shown as sequence 2 in a sequence table. Experiments prove that compared with Oncorhynchus mykiss injected with IHNV (infectious hematopoietic necrosis virus) G (glycoprotein), the level of expression of IgT gene, Mx gene, Viperin gene, CD4 gene, CD8 gene and TNF-alpha gene of Oncorhynchus mykiss injected with the IHNV G and the IL-2 protein is increased substantially, the titer of a neutralizing antibody is increased remarkably, proliferation of lymphocytes is enhanced greatly, and the protective efficacy of the IHNV G is improved effectively. Therefore, the IL-2 protein has important application value in preparation of the animal vaccine adjuvant.

The invention relates to a compound medicine and method for preventing and treating infectious hematopoietic organ necrosis of Masu salmon, belonging to the technical field of aquaculture; 15%~25%, wild chrysanthemum 12%~18%, neem leaves 14%~16%, Atractylodes macrocephala 7%~13%, dried ginger 5%~15%, Houttuynia cordata 3%~7%, licorice 2%~ 8%; specific control methods include aeration of breeding water, methionine iodine bath and disinfection of breeding water, and vitamin C, vitamin E and compound drugs need to be added to the diet. The invention has simple operation, does not affect the quality of aquatic products, and is widely applicable to the prevention and treatment of infectious hematopoietic necrosis of masu salmon.

The invention discloses application of IL-17A protein in preparing animal vaccine adjuvants. The IL-17A protein is protein shown as an amino acid sequence 2 in a sequence table. Experiments prove that compared with rainbow trout injected with infectious hematopoietic necrosis virus G protein, expression level of IgT gene, IgM gene, Mx gene, Viperin gene and CD8 gene of the rainbow trout injected with the infectious hematopoietic necrosis virus G protein and the IL-17A protein is improved remarkably, valence of neutralizing antibody is improved remarkably, proliferation of lymphocytes is remarkably enhanced, and protection force of the infectious hematopoietic necrosis virus G protein is effectively increased. Therefore, the IL-17A protein has important application value in preparing the animal vaccine adjuvants.