Method for increasing blood storage protein (SP) amount of silkworm larvae in early stage
A technology of protein and larvae, applied in animal husbandry and other fields, can solve the problems of few silkworms, a large amount of manpower and material resources, etc., and achieve the effect of simple method, increased yield, and easy implementation
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Embodiment l
[0026] Embodiment 1, a kind of heavy ion ray irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing storage protein SP in the early stage of the 5th instar, and carries out the following steps successively:
[0027] 1. Preparation of larvae of the order Bombyx mori:
[0028] The hatched silkworms were reared with mulberry leaves or artificial feed according to the conventional method until the 3rd day of the 4th instar, and they were set aside.
[0029] 2. Irradiation stimulation (physical stimulation) of hematopoietic organs of silkworm by heavy ion rays:
[0030] First make a hole with a diameter of 3.5mm on a 2.0mm thick acrylic resin plate, align the hole with the left and right sides of the 2nd and 3rd thoracic segments of the 4th instar and 3rd day larvae (where the hematopoietic organs exist), and use scotch tape to The paper fixes the larva part, and uses 30Gy equivalent heavy ion rays (specifically carbon ion...
Embodiment 2
[0046] Embodiment 2, a kind of laser irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing storage protein in the early stage of 5th instar, the following steps are carried out successively:
[0047] 1. Preparation of larvae of the order Bombyx mori:
[0048] The hatched silkworms were reared with mulberry leaves or artificial feed according to the conventional method until the 3rd day of the 4th instar, and they were set aside.
[0049] 2. Laser irradiation stimulation of silkworm hematopoietic organs
[0050]First make a hole with a diameter of 3.5mm on a 2.0mm thick acrylic resin plate, align the hole with the left and right sides of the 2nd and 3rd thoracic segments of the 4th instar and 3rd day larvae (where the hematopoietic organs exist), and use scotch tape to The larvae were partially fixed with paper, and irradiated with a He-Ne laser (wavelength 632nm, output power 32mW, working current 10mW) for 30s stimu...
Embodiment 3
[0062] Embodiment 3, a kind of heavy ion ray irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing and storing protein in the early stage of the 5th instar:
[0063] The larvae of Bombyx mori were changed to the first day of the fifth instar; the blood sampling time was changed to the third day of the fifth instar; the rest were the same as in Example 1.
[0064] The identification results of the target protein in blood are:
[0065] The above-mentioned serum was taken for SDS-PAGE electrophoresis according to the method of Laemmli (1970). The electrophoresis results showed that after local irradiation of hematopoietic organs by heavy ion rays, the silkworm was induced to produce a large amount of two proteins near the blood 70kDa ( image 3 ); after SDS-PAGE, the serum was transferred to PVDF membrane, but the antiserum of silkworm storage protein SP-1 and SP-2 were used for immunoblotting respectively, and the resul...
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