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A method for increasing the amount of protein sp stored in early blood of silkworm larvae

A technology of protein and larvae, applied in animal husbandry, etc., can solve the problems of few silkworms, a large amount of manpower and material resources, and achieve the effect of simple method, increased production and shortened feeding cycle

Active Publication Date: 2020-06-16
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the silkworm itself can produce storage protein, the amount produced by the silkworm in the early 5th instar is very small. Although the silkworm can produce a certain amount of storage protein in the late 5th instar, it requires a lot of manpower and material resources to raise it to the late 5th instar.

Method used

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  • A method for increasing the amount of protein sp stored in early blood of silkworm larvae
  • A method for increasing the amount of protein sp stored in early blood of silkworm larvae
  • A method for increasing the amount of protein sp stored in early blood of silkworm larvae

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Experimental program
Comparison scheme
Effect test

Embodiment l

[0026] Embodiment 1, a kind of heavy ion ray irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing storage protein SP in the early stage of the 5th instar, and carries out the following steps successively:

[0027] 1. Preparation of larvae of the order Bombyx mori:

[0028] The hatched silkworms were reared with mulberry leaves or artificial feed according to the conventional method until the 3rd day of the 4th instar, and they were set aside.

[0029] 2. Irradiation stimulation (physical stimulation) of hematopoietic organs of silkworm by heavy ion rays:

[0030] First make a hole with a diameter of 3.5mm on a 2.0mm thick acrylic resin plate, align the hole with the left and right sides of the 2nd and 3rd thoracic segments of the 4th instar and 3rd day larvae (where the hematopoietic organs exist), and use scotch tape to The paper fixes the larva part, and uses 30Gy equivalent heavy ion rays (specifically carbon ion...

Embodiment 2

[0046] Embodiment 2, a kind of laser irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing storage protein in the early stage of 5th instar, the following steps are carried out successively:

[0047] 1. Preparation of larvae of the order Bombyx mori:

[0048] The hatched silkworms were reared with mulberry leaves or artificial feed according to the conventional method until the 3rd day of the 4th instar, and they were set aside.

[0049] 2. Laser irradiation stimulation of silkworm hematopoietic organs

[0050]First make a hole with a diameter of 3.5mm on a 2.0mm thick acrylic resin plate, align the hole with the left and right sides of the 2nd and 3rd thoracic segments of the 4th instar and 3rd day larvae (where the hematopoietic organs exist), and use scotch tape to The larvae were partially fixed with paper, and irradiated with a He-Ne laser (wavelength 632nm, output power 32mW, working current 10mA) for 30s stimu...

Embodiment 3

[0062] Embodiment 3, a kind of heavy ion ray irradiation stimulates the hematopoietic organ of Bombyx mori larvae to induce the method for massively producing and storing protein in the early stage of the 5th instar:

[0063] The larvae of Bombyx mori were changed to the first day of the fifth instar; the blood sampling time was changed to the third day of the fifth instar; the rest were the same as in Example 1.

[0064] The identification results of the target protein in blood are:

[0065] The above-mentioned serum was taken for SDS-PAGE electrophoresis according to the method of Laemmli (1970). The electrophoresis results showed that after local irradiation of hematopoietic organs by heavy ion rays, the silkworm was induced to produce a large amount of two proteins near the blood 70kDa ( image 3 ); after SDS-PAGE, the serum was transferred to PVDF membrane, but the antiserum of silkworm storage protein SP-1 and SP-2 were used for immunoblotting respectively, and the resul...

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Abstract

The invention discloses a method for increasing blood storage protein (SP) amount of silkworm larvae in an early stage. Positions corresponding to hematopoietic organs of the silkworm larvae in a target stage are exposed to through holes of a separation plate, and the silkworm larvae are stimulated by heavy ion beams or laser irradiation through the through holes. When the heavy ion beams stimulation is carried out, the dose is controlled at 20-40 Gy. When the laser irradiation stimulation is carried out, the irradiation time is 20-80 s. The method better solves the problem that the amount ofthe blood SP of the silkworm larvae is less in the early fifth instar. The left and right sides of second and third thoracic segments of the silkworm larvae (where the hematopoietic organs exist) arephysically stimulated to induce the mass production of the SP in the early fifth instar of the silkworm. The induction treatment can increase the blood SP-1 content by 15-21% and the blood SP-2 content by 14-17%.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for increasing the amount of blood storage protein SP in silkworm larvae, in particular to a method for inducing and increasing the amount of blood storage protein SP in the early fifth instar by physically stimulating the hematopoietic organs of silkworm larvae. Background technique [0002] The silkworm is a model insect of the order Lepidoptera. The blood of the silkworm is responsible for many important physiological functions, and the change of its protein will effectively reveal the physiological and pathological change mechanism of the silkworm; especially in the important period of metamorphosis such as pupae and eclosion, its physiological structure and function will undergo huge changes , and these changes are accomplished by the participation of some important proteins in the blood, especially storage proteins. The research on the function of silkworm blood protein...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/04
CPCA01K67/04
Inventor 屠振力
Owner ZHEJIANG UNIV
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