175 results about "Rainbow trout" patented technology

The invention relates to a technical method of triploid seed production of rainbow trout. The technical method comprises the following steps: in proper time after sperm-egg binding, carrying out thermal (shock) treatment on oosperm in low-temperature environment in a certain time interval, then restoring the original low-temperature environment and carrying out cooling water incubation; leading more than 80% of oosperms which need to be incubated into diploid oosperm to generate triploid oosperm after thermal shock treatment; and raising the triploid oosperm into triploid offspring seed (larval fish). The implementation of the technical proposal stops the discharging of a secondary polar body of the oosperm, induces the oosperm to produce another type of oosperm which is fused by the egg, the sperm nucleus and the secondary polar body with respective monoploid chromosome and contains triploid chromosome, namely triploid oosperm, and realize industrialization of triploid seed production. Each thermal inducing incubator can produce more than 40,000 of triploid oosperms for one time, and each reproduction period (October to February) can produce more than 2,000,000 oosperms. The inductivity of the triploid is more than 80%.

The invention provides a preparation method of game fish triploid fries. The preparation method comprises the following steps of: hybridizing a rainbow trout female parent fish and an arctic char male parent fish; transferring fertilized ova which have been fertilized for 20 minutes under the condition of 7 DEG C into a water body of 26 DEG C and performing heat shock for 20 minutes; then transferring into a water body of 7 DEG C to hatch for about 60 days; and hatching fish fries from the fertilized ova, wherein the eyed rate of the fertilized ova in the hatching period is over 75 percent, the hatching rate of the fries reaches over 90 percent, and the triploid rate of the obtained fries is 90-95 percent through detection of a flow cytometer. According to the preparation method of game fish triploid fries provided by the invention, the problems of sexual precosity, size miniaturization, disease breeding and the like of bred individuals due to long-term close breeding and shortage of systematic breeding in the rainbow trout culturing process are relieved, meanwhile, the hybrid triploid has the two advantages of the hybridization and the triploid, the growth speed and the disease resistance are obviously superior to those of the conventional diploid rainbow trout, and the preparation method has higher economic benefit and market value.

The invention relates to a technical method of artificial propagation of rainbow trout, belonging to the technical field of coldwater fish (salmon and trout) cultivation. The technical method comprises the following steps: step 1, preparing seepage of single salt and the like; step 2, implementing egg washing and artificial insemination of seepage of single salt and the like; step 3, implementing continuous operation of flowing water, absorbing water, expanding and incubation of oosperm in a mesh pot barrel-type incubator; step 4, detecting fertility rate in 4 cell stage; step 5, adopting a mesh cage (or gravel) flat flow type larval fish film-out incubation groove to implement larval fish film-out incubation; step 6, after the larval fish is incubated, removing the mesh cage or the gravel, carrying out larval fish opening training and raising in a primary groove; step 7, after one month of training and raising, removing the larval fish into a hexagonal raising groove, and carrying out offspring seed raising. By adopting continuous operation technology of flowing water, absorbing water, expanding and incubation, the technical method reduces operation procedures and operation tools of stopping water, absorbing water and expanding, and improves the incubation rate by 20%. Compared with the existing embryonic detection technology, the fertility rate detection technology in 4 cell stage is mastered 9 days in advance, thus avoiding the problem that embryonic detection is not accurate.

The invention discloses a compound feed for enhancing color of rainbow trout. The compound feed is prepared from the following raw materials in parts by weight: 0.1-0.3 part of microelements, 0.03-0.06 part of vitamins, 1-2 parts of minerals, 15-25 parts of imported fish powder, 5-10 parts of shrimp shell powder, 2-5 parts of sleeve-fish ointment, 20-40 parts of bean pulp, 10-20 parts of mixed meal, 5-8 parts of corn protein powder, 2-3 parts of alfalfa powder, 0.04-0.08 part of cholate substances, 1-2 parts of spirulina powder, 4-6 parts of kelp powder, 4-6 parts of grease, 0.02-0.04 part of astaxanthin, 0.2-0.4 part of 0.2% lutein and 15-25 parts of flour. The raw materials such as the imported fish powder and the good-quality bean pulp are selected for meeting nutritional indexes of the rainbow trout, and the carotenoid-rich raw materials such as the spirulina powder, the astaxanthin, the lutein and the corn protein powder are emphatically added for enhancing the color of the rainbow trout according to physiological characteristics of the rainbow trout; the compound feed is convenient to use and free from toxic effects, and contains 40%-45% of crude proteins and 12%-16% of crude fat.

The invention provides a bean pulp rainbow trout parr compound feed and a preparation method thereof. Every 100kg of bean pulp rainbow trout parr compound feed is manufactured by 10 to 15kg of steam fish meal, 45 to 55kg of full fat expanded soybean, 10 to 15kg of corn protein powder, 2 to 5kg of isolate soya protein, 3 to 5kg of beer yeast, 0.5 to 1.0kg of Squid paste, 0.5 to 1.0kg of betaine, 3 to 6kg fo flour, 1.5 to 2.5kg of soybean lecithin, 2 to 3kg of fish oil, 2 to 3kg of soybean oil, 0.3 to 0.5kg of methionine, 0.1 to 0.3kg of lysine, 0.3 to 0.5kg of Choline Chloride, 0.5 to 1.0kg of composite vitamin, 0.5 to 1.0kg of composite mineral salt and water. The invention aims at the digesting physiological characteristic during the parr period of the rainbow trout and solves the balancing problem of amino acid by optimizing and adjusting the prescription of the feed, and solves the problems of anti-nutritional factors and palatability by comprehensively using enzyme preparation and feeding promoting agent. The feed of the invention has the advantages of remarkably promoting the quick growth of the rainbow trout parr, improving the surviving rate of the rainbow trout parr, improving the digesting and absorbing rates of the rainbow trout parr on the feed, reducing the drainage of P and N, reducing the culturing cost, and the like.

The invention provides a bean pulp rainbow trout parr compound feed and a preparation method thereof. Every 100kg of bean pulp rainbow trout parr compound feed is manufactured by 6 to 12kg of steam fish meal, 50 to 60kg of expanded bean pulp, 10 to 15kg of corn protein powder, 3 to 5kg of beer yeast, 0.5 to 1.0kg of Squid paste, 0.5 to 1.0kg of betaine, 2 to 6kg fo flour, 1.5 to 2.5kg of soybean lecithin, 5 to 6kg of fish oil, 5 to 63kg of soybean oil, 0.3 to 0.5kg of methionine, 0.1 to 0.3kg of lysine, 0.3 to 0.5kg of Choline Chloride, 0.5 to 1.0kg of composite vitamin, 0.5 to 1.0kg of composite mineral salt and water. The invention aims at the digesting physiological characteristic during the parr period of the rainbow trout and solves the balancing problem of amino acid by optimizing and adjusting the prescription of the feed, and solves the problems of anti-nutritional factors and palatability by comprehensively using enzyme preparation and feeding promoting agent. The invention selects the feed materials with high biological availability, and has the advantages of remarkably promoting the quick growth of the rainbow trout parr, improving the surviving rate of culturing, improving the digesting and absorbing rates of the rainbow trout parr on the feed, reducing the drainage of P and N, reducing the culturing cost, and the like. The materials used by the compound feed of the invention have the advantages of reliable source, stable quality and low cost.

The invention relates to a preparation method of a rainbow trout feed comprising following components in percentage by weight : 18-30 of fish meal, 15-25 of blood meal, 8-18 of bean cake meal, 4-10 of bran, 3-7 of wheaten flour, 4-10 of cornmeal, 6-9 of fish liver and glandular meal, 7-15 of vegetables, 1-3 of feed mineral supplement, 1-3 of vegetable oil and 1-2 of various vitamins. The components are poured into a fish feed stirring mill to be stirred for 20 min in proportion, the mixture is taken out and is poured into a multi-purpose ring model type soft pelleter to be heated and processed into soft particle feed and is then screened by a 40-mesh filter screen, and the weight of each particle is 0.1 g.

The invention relates to a long-distance transport method for fresh cold water fishes. The long-distance transport method is characterized by comprising the following steps of putting the cold water fishes in a fish bag filled with water which is 1 / 5-1 / 4 of the weight of the fishes; fully inflating the fish bag with oxygen, and then, sealing; keeping the fish bag water-tight and air-tight; putting the fish bag in a transport case, and putting 1-2 ice bags in the transport case so as to enable the water temperature in the fish bag to be kept to be 0-18 DEG C during a whole transport process. The fish bag is made of a water-tight and air-tight soft material; the water used in the fish bag is clean spring water or clean river water of which the water temperature is 0-18 DEG C. The long-distance transport method for the fresh cold water fishes provided by the invention is suitable for keep-alive transport of the cold water fishes, such as Chinese sturgeon, Polyodonspathala, salmon, rainbow trout, golden trout, giant salamander (megalobatrachus) and the like, and also suitable for the keep-alive transport of other non-cold water fishes.

A method for inducing the tetraploid salmoniform rainbow trout by hot shock or static water pressure process features that the fertilized ova of rainbow trout is treated by hot shock at 30 deg.C for 5-6 min or static water pressure under 600-650 kg / sq.cm for 5-6 min.

The invention discloses an additive for a soybean pulp type rainbow trout feed. The adding amount of cholesterol in the soybean pulp type rainbow trout feed (a soybean protein accounts for 70 percent of total proteins of the feed) is controlled to be between 0.6 and 1.2 percent. The additive remarkably improves the growth performance and nonspecific immunity of rainbow trout, greatly reduces the using amount of fish meal in the feed, and reduces the cost of the feed.

The invention provides a rainbow trout tetraploid breeding method. Viable tetraploid rainbow trout is obtained in the method by using generic cross of rainbow trout and arcticchar. The rainbow trout and the arcticchar belong to oncorhynchus and salmo respectively; the rainbow trout is sexually matured female; and the arcticchar is sexually matured male. The generic cross of the rainbow trout and the arcticchar is used, fertilized eggs are hatched, young fish is bred and cultured for 2 to 3 years till sexual maturity; partial viable tetraploid exists in the filial generation by detection; and the method provides a feasible path for producing triploid rainbow trout in large scale. Reasonable parameters in the hatching and breeding steps are favorable for growth of larval fish and juvenile fish; the detection method combines cytology detection and chromosome detection of the microscopic level and gonad development condition detection of the macroscopic level, is strict and ordered, and is favorable for accurately judging whether the tetraploid filial generation is generated; and each operation method is simple and feasible, and has good suitability.

The invention belongs to the field of aquaculture, and particularly relates to an application of neutral sterilization electrochemical water in fish egg hatching. The neutral sterilization electrochemical water which is efficient in sterilization, environmentally friendly, safe and free of bacteria, aquatic plants and insects is adopted to hatch fish eggs and hatch eggs of famous fish such as loach, sturgeon, rainbow trout and fancy carp, the fish egg hatchability and the survival rate can be effectively improved, chemicals are replaced, drug residues and aquatic product aquaculture water area environmental pollution are avoided, and the application and method are easy and convenient to operate and fast in efficacy, and have wide application prospects.

A special feedstuff used for hucho taimen larvae relates to a fish feedstuff. The invention solves the problems of low growth speed and high death rate of hucho taimen by feeding the hucho taimen withrainbow trout feedstuff. The hucho taimen special feedstuff comprises the following components in weight percentage: 2-5 percent beer yeast, 2-6 percent wheat-middling, 2-8 percent spray drying bloodflour, 60-70 percent fish flour, 10-20 percent fish oil, 1-4 percent soybean lecithin, 0.2-0.8 percent sodium alginate, 0.01-0.05 percent L-carnitine, 0.05-0.2 percent KCL, 0.02-0.1 percent inositol,0.02-0.07 percent enzyme preparation, 0.01-0.04 percent high stability VC, 0.1-0.5 percent magnesite, 0.01-0.05 percent antioxidant, 0.05-0.02 percent Kemei, 0.8-1.5 percent zeolite. Compared the special feedstuff with the imported rainbow trout feedstuff, by feeding the hucho taimen with the special feedstuff, the survival rate of the hucho taimen can reach above 95% after 4 weeks, and the survivalrate of the hucho taimen can reach above 92% after 8 weeks; the average weight is increased by 18.35%, and the weight gain rate is improved by 9.49%.

The invention discloses Saccharomyces cerevisiae engineering bacteria for expressing recombinant rainbow trout antibiotic peptide Oncorhyncin II and the preparation method thereof. The preparation method comprises the steps as follows: introducing the coding genes of the mature peptide of the rainbow trout antibiotic peptide Oncorhyncin II into the Saccharomyces cerevisiae to obtain the strains capable of secretion-expressing the rainbow trout antibiotic peptide Oncorhyncin II. The Saccharomyces cerevisiae rainbow trout antibiotic peptide Oncorhyncin II is preserved in China General Microbiological Culture Collection Center on June 19th, 2008 with the preservation number of CGMCC No.2554. The Saccharomyces cerevisiae engineering bacteria can stably express the recombinant rainbow trout antibiotic peptide Oncorhyncin II, which has antibacterial activity to gram positive bacteria and gram negative bacteria, such as Escherichia coli, Staphylococcus aureusetc, and can be used for preparing feed additives, fresh-keeping agents, cosmetics and pharmaceutical products.

The invention relates to application of rubber seed oil to rainbow trout compound feed and a formula of the rubber seed oil, and belongs to the technical field of aquaculture. The rubber seed oil is substituted for fish oil partially to be added into the rainbow trout compound feed. The compound feed comprises, by weight, 16-20% of fish meal, 28-32% of bean pulp, 8-12% of cottonseed meal, 13-17% of rapeseed dregs, 6-10% of corn gluten meal, 1-4% of high gluten flour, 4-12% of the rubber seed oil, 0-8% of the fish oil, 0.3-0.5% of soya bean lecithin, 0.7-1.2% of monocalcium phosphate, 0.1-0.3% of choline chloride, 0.02-0.05% of vitamin C phosphate, 0.01-0.03% of ethoxyquin, 0.5-1.0% of mineral substance premix and 0.5-1.0% of vitamin premix. The sum of weight percentages of all raw materials is 100%. The application of the rubber seed oil to the rainbow trout compound feed and the formula of the rubber seed oil have the advantages that the rubber seed oil is fully utilized to serve as a fat source in the rainbow trout compound feed, the fish oil as feed grease in short supply is saved, and the compound feed has no bad influence on rainbow trout; formula cost and feed factors are reduced, high cost performance is achieved, and culture benefit of the rainbow trout is improved.

The invention belongs to the technical field of fish culture, and particularly discloses a breeding method for prolonging the service life of rainbow trout. The method comprises the following steps that 1, an aquaculture pond is selected; 2, rainbow trout fry are put; 3, culture management is carried out; 4, feed is put at the advanced fry stage; 5, water management is carried out. The rainbow trout is cultured through the culture method, the disease resistance of the rainbow trout can be enhanced, the survival rate is greatly increased, the obtained rainbow trout is fat, delicious and tender in meat, and the economic value is extremely high.

The invention aims at providing a method for breeding full-feminization rainbow trout. The method comprises the steps of first feeding a mixed feed containing a feed for common fishes and letrozole and then feeding the feed for common fishes in the breeding of first upward floating fries obtained in first fertilized ovum incubation; taking sperms of false milter with female genotype and male phenotype from adult fish to perform second fertilization together with ovum of the rainbow trout, and finally obtaining second adult fish, namely the full-feminization rainbow trout. The mixed feed containing the letrozole is used in the first feeding process, thereby facilitating control of gender of larval fish. Parameters of the method are reasonably set in two-time incubation and feeding steps, and various factors produce synergistic effect, thereby facilitating the incubation of the fertilized ovum to obtain upward floating fries and enabling the upward floating fries to grow to be adult fish. Various operation methods in the method are simple, convenient and easy to perform, and the method is good in adaptability and suitable for being popularized and used in vast numbers of farmers.

The invention discloses a biosensing detection method used for identifying a salmo salar and a rainbow trout. The biosensing detection method comprises the following steps: the step 1 of extracting genome DNA of a sample to be detected, using a specific primer pair of the salmo salar to carry out PCR reaction with the genome DNA of the sample to be detected as a template and obtaining a PCR amplification product I of the sample to be detected; the step 2 of adding the PCR amplification product I obtained in the step 1 to the surface of an electrode decorated by an anchoring acceptor for reaction; the step 3 of detecting an electrochemistry signal of an electrochemistry activity labeled molecule and deciding that the sample to be detected comes from the salmo salar if the electrochemistry signal of the electrochemistry labeled molecule exists. The specific primer pair of the salmo salar comprises the electrochemistry activity labeled molecule and an anchoring labeled molecule on the upstream of a base sequence respectively, and the anchoring labeled molecule can be captured by the anchoring acceptor to enable the PCR amplification product with the anchoring labeled molecule to be captured by the surface of the electrode. The method is suitable for fish samples of any growth stage and in any shape.

The invention relates to a breeding method of rainbow trout. The breeding method mainly comprises the following steps: (1) feeding fries with a first mixed feed for 4-6 months, wherein the first mixed feed comprises a first conventional feed and a first additive, and the ratio of the first conventional feed to the first additive is 1000:(15-35); (2) breeding in the microflow under the breeding condition that the water temperature is 9-11 DEG C in the step (1), wherein the pH value is 7-8, and the dissolved oxygen (DO) value is 6.5-9.5mg / L; (3) after the feeding of the first mixed feed is finished, feeding with second mixed feed, wherein the second mixed feed comprises second conventional feed and a second additive, and the ratio of the second conventional feed to the second additive is 1000:(10-20); and (4) breeding in the microflow under the breeding condition that the water temperature is 7-15 DEG C in the step (3), wherein the pH value is 6.6-9, and the dissolved oxygen (DO) value is 6-10mg / L. The breeding method is suitable for the growth of the rainbow trout, and is capable of improving the immunity and reducing the disease rate; the mixed feeds are reasonable in nutritive proportion and are capable of improving the appetite and feed intake of fish.

The invention discloses a breeding and hatching device for salmon and trout. The salmon and the trout comprise chum salmon, salmon, golden trout, rainbow trout and the like, and have delicious meat and a wide development prospect, and particularly eggs of the salmon and the trout have high nutritional value and economic value. Most of the eggs are demersal and have large granules, and the conventional organic glass cylinder hatcher and glass reinforced plastic tank hatcher cannot meet the requirements of hatching and breeding the salmon and the trout. The breeding and hatching device for the salmon and the trout comprises a support (1) which is provided with a rail, wherein the rail is connected with a rail sliding block (2) which supports a hatching tank (3); a water spray pipe (4) and a water supply pipe (5) are arranged above the hatching tank (3); a support plate (12) is arranged below the hatching tank (3); an ultraviolet lamp ozone generator (6) is arranged below the support plate (12); and a circulating water tank (7) is arranged below the ultraviolet lamp ozone generator (6). The breeding and hatching device is used for breeding and hatching the salmon and the trout.

The invention discloses a mass spectrometry identification method for salmo salar and rainbow trout. The method includes the following steps: a) obtaining the amino acid sequence of salmo salar; b) obtaining the amino acid sequence of rainbow trout; c) utilizing a specific enzymatic method to cut to-be-detected protein into a small molecular weight polypeptide fragment mixture, utilizing an electrostatic field orbitrap high resolution mass spectrometry full scan mode to detect the molecular weight and fragment information of polypeptide in the mixture, performing comparison with an amino acidsequence obtained before, and qualitatively judging whether the characteristic peptide fragments of the salmo salar and rainbow trout exist; and d) selecting the characteristic peptide fragment determined as the salmo salar or the rainbow trout, and utilizing tandem quadrupole mass spectrometry to establish a multi-reaction monitoring method to perform quantitative analysis. Identification can beperformed by utilizing the protein characteristic peptide fragments, so that the qualitative identification and adulteration quantification of the salmo salar and rainbow trout can be simultaneously realized; and minimum detection limit can reach 1%, so that the method is high in sensitivity, high in accuracy and strong in anti-interference ability, and can be widely applied to the identificationof the salmo salar.

The invention belongs to the technical field of fishing culture, and particularly discloses a culture method of rainbow trout. The method includes the following steps: (1) selection of fishpond; (2) preparation of solutions, such as single salt; (3) spawn collection, milt collection, spawn washing and insemination; (4) fertilized eggs hatch; (5) fry rearing; (6) rainbow trout fry input; (7) fish feed discharging in the juvenile stage; (8) fish feed discharging in the adult stage; (9) water body management. By applying the culture method of rainbow trout, the rainbow trout has great economic values with strong disease resistance, high survival rate and succulent and tender meat quality.

The invention provides an Oncorhynchus mykiss fingerling forage. The Oncorhynchus mykiss fingerling forage comprises 55-65 parts of Peruvian fishmeal, 8-12 parts of soybean meal, 2-8 parts of wheat bran, 8-12 parts of barley flour, 2-8 parts of corn flour, 2-8 parts of powdered Leaf of Japanese Pagoda tree, 2-8 parts of beer yeast, 2-4 parts of soybean oil, 0.5-1.5 parts of mineral matters, 0.05-0.15 parts of multiple vitamins and 4-6 parts of phosphatide. The Oncorhynchus mykiss fingerling forage has the advantages of carrying of nutrient components suitable for Oncorhynchus mykiss fingerlings, balanced energy and proteins, and high forage conversion rate.

The invention relates to a rainbow trout feed, which is prepared with the following raw materials by weight percent: 18-30 percent of loach powder, 15-25 percent of duck blood meal, 8-18 percent of rapeseed cake meal, 4-10 percent of bran, 3-7 percent of sorghum powder, 4-10 percent of soybean meal, 6-9 percent of river snail powder, 7-15 percent of fresh cabbage, 1-3 percent of mineral additive, 1-3 percent of animal oil and 1-2 percent of vitamins. The rainbow trout feed is prepared through the following steps: pouring the materials into a fish feed mixer according to a proportion, mixing for 30 minutes, taking out the mixture, pouring the mixture into a multi-purpose ring-mode type soft-particle granulator for fish feed, heating to produce soft-particle feed, and then screening with a 80-mesh sieve, thereby obtaining the product. The weight of each piece of rainbow trout feed is 0.2g.

The invention discloses an intestinal microbial preparation suitable for cold water rainbow trout. The preparation comprises clostridium butyricum, bacillus coagulans, and radix astragali extract andChinese gall extract which come from Chinese herbal medicines. The radix astragali extract and Chinese gall extract are used as a probiotic adjuvant. Each kilogram of the preparation contains 6.5x10<10> to 8.5x10<14> CFU of the viable clostridium butyricum, 2.5x10<10> to 4.5x10<14> CFU of the viable bacillus coagulans, and 25-50g of the radix astragali extract and the Chinese gall extract. According to the intestinal micro-ecological characteristics of the cold water rainbow trout, the technology of the invention can improve intestinal tissue structure, improve intestinal microbial functions of the cold water rainbow trout, maintain intestinal microbial flora balance, increase the breeding of beneficial bacteria, reduce the colonization of harmful bacteria, effectively regulate and promotethe metabolism of intestinal microorganisms, and indirectly and targetedly enhance the body immune functions of the cold water rainbow trout. In addition, the micro-ecological balance regulation compound preparation has the advantages of high efficiency, safety, no residue, and no drug resistance.

The invention discloses a method for culturing and transporting fresh fishes by adopting electrified water for fishing. The method is characterized by comprising the following steps of screening fishes; preparing the electrified water for fishing; transporting the fishes; establishing a culture pond for culturing the fresh fishes; distributing the fresh fishes to a destination. According to the method provided by the invention, fresh grass carp, crucian, chub, carp, black carp, bighead carp, channel catfish, catfish, sturgeon, rainbow trout and other commercial fishes are cultured and transported by using safe, high-efficiency and no-pollution electrified water for fishing; bacteria in body surfaces of the fresh fishes and in a purified water body are effectively purified, and the generation of ammonia nitrogen and nitrite is inhibited; the culturing and transporting survival rate of the fresh fishes is improved, and the quality of the fresh fishes is improved. The method is simple in process and has great application potential.

The invention discloses a breeding method of rainbow trout triploids through hydrostatic pressure induction. A certain time interval is selected at a proper time after sperm-egg binding so as to carryout hydrostatic pressure treatment with certain intensity on fertilized eggs, and then a normal environment is recovered for conventional incubation, so that the fertilized eggs which can be bred into diploids generate 100% triploid fertilized eggs after hydrostatic pressure treatment, and then the triploid fertilized eggs are bred into triploid fry. The method has the advantages that a built rainbow trout triploid breeding technical method can be used for generating the triploid rainbow trout fry through efficient batch induction, the induction rate of the rainbow trout triploids is up to 100%, and the survival rate of the rainbow trout triploids is not obviously different from that of diploid rainbow trout. The method is easy to operate, high in practicability, easy to implement, safe and reliable, and provides technical support for large-scale production of the rainbow trout triploids.

The invention provides infectious hematopoietic necrosis (IHN) nucleic acid vaccines for Chinese rainbow trout and application thereof. A preparation method of the IHN nucleic acid vaccines for Chinese rainbow trout includes the following steps that firstly, specific primers for amplifying genotype J IHN virus surface glycoprotein genes (Glycoprotein, G) are designed; secondly, IHN virus isolates are cultured with a virus sensitive cell line, and then virus genome RNA is extracted; thirdly, the virus genome RNA obtained in the second step is subjected to one-step RT-PCR amplification with the primers obtained in the first step; fourthly, a recovery product obtained in the third step is connected with a pMD19-T simple vector, a connection product is converted into DH5alpha competent cells, single colonies are picked out, plasmids are extracted for PCR identification after amplified culture, and plasmids identified to be correct are subjected to sequencing; fifthly, IHNV isolate glycoprotein genes identified to be correct in the fourth step are cloned to a eukaryotic expression vector pcDNA3.1 with BamH I and Xho I restriction enzyme cutting sites to establish the nucleic acid vaccines.

The invention relates to the field of the aquaculture feed and in particular relates to a selenium-enriched rainbow trout feed, and a preparation method and an application thereof. The feed provided by the invention comprises Peru fish meal, swine blood meal, euphausiid shrimp meal, chicken meal, corn gluten meal, bean pulp, soybean protein isolate, vital gluten, pregelatinized starch, sleeve-fish oil, soya bean lecithin, glycine betaine, choline chloride, a mineral substance premix, a vitamin premix and yeast selenium. By feeding rainbow trout with the feed provided by the invention for 70 days, the total selenium content (wet weight) in muscles of the rainbow trout can reach 0.57-1.12mg / kg. The total selenium content (wet weight) of full fish can reach 0.58-1.06mg / kg. The rainbow trout bred by the feed is better in growth performance, feed nutrient gain rate and muscle quality. In addition, the selenium-enriched rainbow trout feed also has the functions of protecting liver and gallbladder and enhancing the oxidation resistance of rainbow trout and non-specific immune function.

Rainbow trout juvenile fish compound feed with low nitrogen and phosphorus emission is prepared from, by weight, 35-50 parts of compound protein component, 10-25 parts of compound grease compound, 1-5 parts of compound Chinese herbal medicine component, 2-5 parts of algae powder special component, 1-5 parts of microecological preparation, 10-15 parts of buckwheat middling, 10-15 parts of whole oat powder, 0.02-0.1 part of vitamin mixture and 0.1-0.5 part of mineral substance mixture. The feed can meet the requirements of rainbow trout juvenile fish for high protein, conform to the characteristics of sarcophagi of rainbow trout juvenile fish, promote growth, reduce nitrogen and phosphorus emission and improve the breeding efficiency.