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Intestinal microbial preparation suitable for cold water rainbow trout

A preparation, cold water technology, applied in the field of intestinal microbial preparations, can solve problems such as the effect of the formulation, not combined with the intestinal system and immune system, etc., to promote multi-level effects, inhibit the growth of pathogenic bacteria and other harmful bacteria, and regulate functions. Effect

Active Publication Date: 2018-04-20
MARINE BIOLOGY INST OF SHANDONG PROVINCE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The currently disclosed probiotics are all different single strains, and do not combine the characteristics of cold-water cultured animals, pathogenic bacteria and Chinese herbal medicine preparations, nor discuss the targeted effect of the formula in combination with the intestinal system and immune system

Method used

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  • Intestinal microbial preparation suitable for cold water rainbow trout
  • Intestinal microbial preparation suitable for cold water rainbow trout
  • Intestinal microbial preparation suitable for cold water rainbow trout

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Analysis of the intestinal microbial flora structure of rainbow trout

[0014] After the intestinal microorganisms of rainbow trout were separated and purely cultivated on a plate, they were identified by physiological and biochemical tests such as colony color, Gram staining, bacterial shape, motility, glucose oxidative fermentation, and oxidase, and the bacterial species obtained by analysis were Micrococcus, Acinetobacter, Corynebacterium, Aeromonas, Staphylococcus, Pseudomonas, Bacillus, etc. The abundance of intestinal microbial flora was analyzed by high-throughput sequencing technology, and the core flora in the intestinal tract was composed of four phyla of bacteria: Bacteroidetes, Proteobacteria, Firmicutes and Actinomycetes; The bacterial groups with the highest abundance on the mucous membrane belong to the genus Acinetobacter, Cetella, Pseudomonas and Psychrobacter, while Aeromonas, Clostridium and other types of bacteria are less, which cannot fu...

Embodiment 2

[0020] Example 2: Inhibitory effect of Chinese herbal medicine extracts on pathogenic bacteria Aeromonas salmonicida

[0021] The antibacterial effect of Chinese herbal medicine extracts on pathogenic bacteria was screened by Oxford cup inhibition zone test. Aeromonas salmonicida is activated by BHI medium, and the activated Aeromonas salmonicida is coated with TSA plate (150 μl per plate); take the center of the medium plate coated with bacteria as the center point, according to the side length of 4cm, etc. Place a sterile Oxford cup on the side of the triangle (to ensure that the bottom layer is not penetrated), put three Oxford cups on each plate, add different Chinese herbal extracts (drug concentration: 0.1mg / ml, adding volume: 50μl) into the Oxford cups, overnight at 28°C to cultivate.

[0022] Among them, the astragalus extract is prepared by pulverizing the Chinese herbal medicine astragalus into powder with a high-speed pulverizer, extracting 4 times at 60° C. for 1 ...

Embodiment 3

[0026] Embodiment 3: the preparation method of microbial preparation

[0027] Bacillus coagulans and Clostridium butyricum were activated overnight, transferred to fresh medium, cultured overnight at 30°C, and the concentration of Clostridium butyricum was diluted with sterile saline to 6.5×10 10 ~8.5×10 14 CFU, Bacillus coagulans is 2.5×10 10 ~4.5×10 14 CFU, soaked in diluted bacterial solution for 1 hour per kilogram of feed, aired in the shade, and dried for later use. Weigh 25-50 grams of Radix Astragali and Galla gall extracts, dissolve and dilute them with sterile water at a ratio of 1:100, soak in the diluted solution for 1 hour per kilogram of feed, dry in the shade, and dry for later use.

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Abstract

The invention discloses an intestinal microbial preparation suitable for cold water rainbow trout. The preparation comprises clostridium butyricum, bacillus coagulans, and radix astragali extract andChinese gall extract which come from Chinese herbal medicines. The radix astragali extract and Chinese gall extract are used as a probiotic adjuvant. Each kilogram of the preparation contains 6.5x10<10> to 8.5x10<14> CFU of the viable clostridium butyricum, 2.5x10<10> to 4.5x10<14> CFU of the viable bacillus coagulans, and 25-50g of the radix astragali extract and the Chinese gall extract. According to the intestinal micro-ecological characteristics of the cold water rainbow trout, the technology of the invention can improve intestinal tissue structure, improve intestinal microbial functions of the cold water rainbow trout, maintain intestinal microbial flora balance, increase the breeding of beneficial bacteria, reduce the colonization of harmful bacteria, effectively regulate and promotethe metabolism of intestinal microorganisms, and indirectly and targetedly enhance the body immune functions of the cold water rainbow trout. In addition, the micro-ecological balance regulation compound preparation has the advantages of high efficiency, safety, no residue, and no drug resistance.

Description

technical field [0001] The invention belongs to the technical field of feed additives, and in particular relates to an intestinal microbial preparation suitable for cold-water rainbow trout. Background technique [0002] There is a huge cold water mass in the Yellow Sea of ​​my country, the water quality is good, and it has the conditions to develop high-quality cold-water fish farming industry. The main problem restricting the development of my country's high-quality low-temperature fish farming industry is that frequent breeding diseases and high water temperature in the breeding sea area are not suitable for breeding high-quality low-temperature-resistant fish. [0003] Probiotics are a class of live bacterial preparations that play a beneficial role by improving the ecological balance of the host's intestinal flora, thereby improving the health level and state of the host. Aquaculture probiotics are microbial preparations with no toxic side effects and no drug resistanc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/80A23K10/18A23K10/30
CPCA23K10/18A23K10/30A23K50/80Y02A40/818
Inventor 樊英叶海斌刁菁严芳李乐王晓璐盖春蕾于晓清李天保许拉王淑娴
Owner MARINE BIOLOGY INST OF SHANDONG PROVINCE
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