36 results about "Proteobacteria" patented technology

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses a preparation method for a dichloro substituted II-type halogenated polyketone compound zunyimycin A, wherein the compound is derived from a secondary metabolite of a streptomyces sp.FJS31-2 strain (with the strain preservation number of CGMCC4.7321), and finally, a structure is identified and determined after strain activation, expansion culture and isolation. The compoundcan be used in treatment of diseases infected by gram-positive bacteria such as staphylococcus aureus (MRSA) and drug-resistant bacteria thereof, staphylococcus epidermidis, bacillus subtilis and thelike, is used in treatment of diseases infected by gram-negative bacteria such as super-spectrum beta-lactamase escherichia coli (ESBL), proteusbacillus vulgaris, bacterium burgeri and the like, andis used for treatment of diseases infected by fungi, such as candida albicans.

The invention discloses broad bean rhizobium with drought tolerance and application of the broad bean rhizobium. The separated broad bean rhizobium strain with drought tolerance is obtained from a broad bean host, the strain is subjected to shape, physical and chemical property and whole genome analysis and identification, the strain belongs to a new strain in the bacteria field, proteobacteria, alpha-proteobacteria, rhizobium, rhizobiaceae and rhizobium, and the microbial preservation number of the strain is CGMCC No.24092. According to the broad bean rhizobium provided by the invention, PEG (Polyethylene Glycol) is used for simulating drought stress, a broad bean artificial water control potted plant drought test and a rain-fed agricultural area field inoculation verification test prove that the broad bean rhizobium provided by the invention has the characteristics of drought tolerance, efficient nodulation, strong nitrogen fixation capability and the like; and the broad bean biomass and yield are increased, and the broad bean has application prospects in the aspects of improving the stress resistance of leguminous crops, promoting growth, improving the yield and the like.

The present invention discloses a method for further denitrogenation by using wood and rotten wood. The method comprises: S1, continuously soaking rotten wood for 20-30 h by using a hydrochloric acid solution with a concentration of 9-10%, taking the rotten wood out, and rinsing; S3, placing the rotten wood into an anaerobic ammonium oxidation base inorganic salt solution to soak for 28-32 days; and S5, placing the rotten wood in a denitrogenation tank to carry out bacterial culture, maintaining the temperature in the denitrogenation tank at 20-30 DEG C, maintaining the oxygen concentration and the like of 5-8 mg/L, discharging sewage into the denitrogenation tank, and gradually growing the microbial flora on the rotten wood, wherein one-step denitrogenation can be achieved when the proportion of delta-Proteobacteria achieves more than 50%. According to the present invention, the rotten wood is adopted to create the nature parasitism environment for the anaerobic bacteria, and the survivability and the propagation speed of the bacteria are improved; and any chemical or organic substances are not required to be added during the denitrogenation process, aeration is not required, and the bacteria in the sewage are directly utilized to carry out back-bite decontamination, such that raw material consumption is reduced, and manpower and resources are saved so as to reduce cost.

The invention discloses a composition based on brown algae extract and lactobacillus reuteri and application of the composition. The composition disclosed by the invention is prepared from the following components in parts by weight: 30 to 97 parts of brown algae extract and 3 to 70 parts of lactobacillus reuteri. The combination of the brown algae extract and the lactobacillus reuteri can promote field planting of the lactobacillus reuteri in intestinal tracts, and the field planting rate is remarkably increased. The combination of the brown algae extract and the lactobacillus reuteri can significantly reduce the fasting blood glucose and glycosylated hemoglobin concentration of type 2 diabetes model mice, improve the insulin secretion amount, significantly improve the abundance of beneficial bacteria such as bifidobacterium and lactobacillus in intestines, reduce the abundance of conditioned pathogenic bacteria such as enterococcus and proteobacteria. Good application prospects are realized.

The invention discloses a method for evaluating the ecotoxicological impact of microplastics. The method includes the following steps that (1) artemia exposure experiment is performed; (2) intestinaltract collecting is performed; (3) DNA extraction and PCR amplification are performed; (4) Illumina Miseq sequencing is performed; (5) data processing is performed; (6) data analysis is performed, specifically, cluster analysis of the processed data finds that the main phylums in each group at the phylum level are Actinobacteria and Proteobacteria; and (7) ecotoxicology evaluation is performed, specifically, the microplastics have a certain toxic effect on organisms. The method is simple in operation, high in accuracy and short in experimental period, and can rapidly detect intestinal microorganisms. The method can quickly and accurately evaluate the ecotoxicological impact of the microplastics.

The invention discloses a feed for inhibiting intestinal proteobacteria of triploid rainbow trout, belongs to the technical field of aquatic feeds, and relates to a feed for improving intestinal floraof the triploid rainbow trout. The feed provided by the invention is to solve the technical problem that of the intestinal proteobacterium diseases of the triploid rainbow trout are epidemic with enlargement of a culture scale and reinforcement of a culture density and continuous increase of environmental stress. The feed is composed of the following components in parts by weight: 200 parts of wheat middling, 398 parts of fish meal, 290 parts of soybean meal, 60 parts of fish oil, 20 parts of phospholipid oil, 10 parts of monocalcium phosphate, 3 parts of a vitamin premix, 2 parts of a traceelement premix, 2 parts of choline chloride, 5-10 parts of xylooligosaccharide and 0-5 parts of cellulose. The method provided by the invention has the advantages of simple and scientific steps, reasonable formula and the like. The feed disclosed by the invention can inhibit the intestinal proteobacteria of the triploid rainbow trout, so intestinal health is improved, and rapid growth is promoted.According to the feed disclosed by the invention, in breeding practice, the feed coefficient is reduced by 0.08, and the growth speed is increased by 0.7%-9%.

The invention provides a method for improving gas yield by adding sodium propionate to domesticate microbial communities of an anaerobic fermentation system and application of the method, and belongs to the technical field of environmental protection. According to the method, sodium propionate with different concentrations is added into biogas residues subjected to anaerobic fermentation, and then culture is performed through a culture medium, so that the conclusion that the yield of methane is obviously and regularly changed and the diversity and richness of microbial communities are respectively improved along with the increase of the concentration of sodium propionate is obtained; meanwhile, the optimal sodium propionate concentration, the optimal fermentation time and the like are determined when the methane daily yield and the methane cumulative yield are the highest, and the relative abundance of the firmicobacteria bacteria, the proteobacteria bacteria, the methanosarcina archaea and the methanomycorrhiza archaea is the maximum; therefore, the purposes of accurately controlling and remarkably increasing the methane yield and improving the microbial community composition are achieved.