33 results about "Salmonella sps" patented technology

There are provided novel synthetic stool preparations comprising bacteria isolated from a fecal sample from a healthy donor. The synthetic stool preparations are used for treating disorders of the gastrointestinal tract, including dysbiosis, Clostridium difficile infection and recurrent Clostridium difficile infection, prevention of recurrence of Clostridium difficile infection, treatment of Crohn's disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, and diverticular disease, and treatment of food poisoning such as salmonella. Methods of preparation and methods of use of the synthetic stool preparations are also provided.

The invention provides a Bacillus coagulans HEW-B379 with a probiotic effect. The above strain is named as HEW-B379, and the preservation number of the strain is CGMCC No.12553. The Bacillus coagulans HEW-B379 has a substantial probiotic property, and can effectively inhibit growth breeding of enteropathogenic Escherichia coli, Staphylococcus aureus, Salmonella typhi, salmonella, Shigella, Proteus species, Shewanella putrefaciens and Pseudomonas aeruginosa. The Bacillus coagulans HEW-B379 has strong stress resistance, can resist high temperature and simulated gastric juice and simulate bile salt environment, can keep the survival rate of 99-100%, and can effectively adjust microbial balance of animal intestinal tracts, inhibit growth of harmful microbes, promote nutrition absorption of animals, improve the conversion rate of a feed and improve the productivity of the animals.

The invention discloses preparation and application of Clostridium butyricum and a live Clostridium butyricum preparation; Clostridium butyricum LXKJ-1 is collected in China Center for Type Culture Collection on 24 March, 2016 under CCTCC NO: M201613. A seed medium, fermentation medium formulation, culture conditions for Clostridium butyricum and a production of a preparation of live Clostridium butyricum are also disclosed. The Clostridium butyricum provided herein is tolerant to acids, bases and high temperature, is highly capable of producing butyric acid, can inhibit animal pathogens, such as Escherichia coli, Salmonella, Shigella, Staphylococcus aureus, Listeria monocytogenes, and Clostridium perfringens, and can prevent diarrhea in livestock and poultry due to Escherichia coli, Salmonella and Clostridium perfringens, improving intestinal flora balance, promoting the growth of livestock and poultry, relieving constipation in sows, improving egg weight for layers, improving eggshell quality for layers, and decreasing egg-feed ratio.

Disclosed herein are is a novel bacteriophage which has specific bactericidal activity against one or more Salmonella bacteria selected from the group consisting of Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum, and Salmonella Pullorum without affecting beneficial bacteria. Disclosed are also compositions, animal feeds or drinking water, cleaners and sanitizers for preventing and treating the infectious diseases caused by Salmonella Enteritidis, Salmonella Typhimurium, Salmonella Gallinarum or Salmonella Pullorum including salmonellosis, Salmonella food poisoning, Fowl Typhoid, and Pullorum disease or for controlling the salmonella bacteria.

The invention discloses a salmonella phage LPSE34. The preservation number of the salmonella phage LPSE34 is CCTCC NO 2018121. The phage is a broad-spectrum salmonella phage and can crack drug-tolerant salmonellas, it is identified that the phage belongs to myoviridae, and the phage is named as LPSE34. The phage LPSE34 is stable in titer at the pH of 4-12 and the temperature of 30-60 DEG C. The invention further discloses application of the salmonella phage in food, especially in chicken and ham systems; and in addition, the invention further discloses a compound edible coating film formed bytaking the phage as an antibacterial substance to be combined with sodium alginate, and compared with antibiotics and chemical preservatives, the phage cannot affect the texture and flavor of food andhas the characteristics of high specificity, no residue and safety. The salmonella phage LPSE34 can serve as a bacteriocidal substance / the antibacterial substance to control pollution of food-borne pathogens to the food and is a very promising biological agent for guaranteeing the food safety.

The invention discloses a detection method for salmonella enteritidis and a detection kit. A salmonella enteritidis specific aptamer is taken as a molecular recognition element, a stem-loop structure nucleic acid H1 is opened under the action of salmonella enteritidis to undergo interaction with a stem-loop structure nucleic acid H2 so as to form partial double-stranded DNA, which includes endonuclease recognition sequences, H2 is incised under the action of endonuclease to release G basic group-rich nucleotide sequences. Under the action of Hemin, a G tetramer structure with similar HRP catalytic activity is formed, can achieve catalytic oxidation of chromogenic reaction to turn colourless substrate into blue. The method provided by the invention has high sensitivity, has a detection limit is 10 cfu / mL, also has good specificity, and other common bacteria have no influence on detection. The whole detection process can be completed at room temperature. The method has the advantages of simple operation, economical efficiency and cheapness, etc. The detection result can be directly observable, and no detecting instrument is sneeded. The method can detect living bacteria directly, and has no need for lysis of bacteria.

The invention specifically discloses a microbial inoculant, in particular to a microbial inoculant prepared from swine borne bacillus licheniformis YHSH-02 and the application thereof in livestock breeding. The culture is preserved in CGMCC, 3#, Yard #1, West Beichen Road, Chaoyang District, Beijing, with the preservation number of CGMCC NO. 15454 and the preservation date of 15th, March, 2018. The bacillus licheniformis YHSH-02 is separated from a swine intestinal tract, can generate rich lipopeptides bacilli, antifungal proteins and the like, and can inhibit escherichia coli, pseudomonas aeruginosa, enterococcus faecalis, enterobacter cloacae, pseudomonas aeruginosa, proteusbacillus vulgaris, staphylococcus aureus, salmonella and clostridium. The bacillus licheniformis YHSH-02 obtained from the swine intestinal tract is a biological control potential culture which is high in preventing and treating efficiency and wide in preventing and treating range and can promote growth obviously,and has a good application and development prospect in non-resistant breeding.

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

The invention relates to a fowl salmonella gallinarum attenuated isolated strain and application thereof. The isolated strain is preserved in the China General Microbiological Culture Collection Center, Beijing, China, on July 2, 2018, has a preservation number of CGMCC No.16049, and is classified and named as Salmonella Gallinarum. The strain is attenuated to chicken of different ages, is apathogenic, and can be used as a strain vector for preventing livestock salmonella gallinarum infection and preventing infectious diseases of livestock (chicken). The fowl salmonella gallinarum attenuated isolated strain provides data reference to evaluating the toxicity of salmonella attenuated vaccine strains and potential as the strain vector of livestock (chicken) live vaccines.

The invention discloses a salmonella bacteriophage and bacteriophage anti-bacterium composition and an application thereof. A bacteriophage is salmonella enteritidis bacteriophage LPSE1 and salmonella typhimurium bacteriophage LPST10, and both the salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have excellent inhibiting effect on salmonellas and good tolerance for pH (potential of hydrogen) and temperature. Application examples of the salmonella bacteriophage to food systems verify that the bacteriophage has excellent effect when being used for bacteriostatic agents. The salmonella enteritidis bacteriophage LPSE1 and the salmonella typhimurium bacteriophage LPST10 have synergistic effects after being mixed and have more beneficial inhibiting effect as compared with a single bacteriophage.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC4) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6786. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect of promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect on effectively protecting the SPF chickens.

The invention relates to a Listeria monocytogenes monoclonal antibody hybridoma cell strain and an application thereof and belongs to the technical field of food safety immunological detection. According to the invention, a complete antigen of a specific polypeptide of Listeria monocytogenes is uniformly mixed with an equal volume of Freund adjuvant (Sigma), BALB / c mice are immunized by subcutaneous injection, after the BALB / c mice are immunized for four times, spleen cells of immunized mice are fused with mice myeloma cells of immunized mice by virtue of a PEG method, an indirect ELISA screening and three times of subcloning are carried out to obtain a monoclonal cell strain A. The monoclonal antibody secreted by virtue of the monoclonal cell strain A has cross reaction with different Listeria monocytogenes culture supernatants and no cross reaction with listeria except Listeria monocytogenes, E. coliO 157, Salmonella and Campylobacter jejuni and thus the Listeria monocytogenes monoclonal antibody hybridoma cell strain can be used in the specific detection of Listeria monocytogenes in a food.

The invention relates to a nanometer antibody for resisting salmonella enteritidis and application thereof. The antibody has the function of being combined with the salmonella enteritidis. The invention also discloses the nanometer antibody and a gene sequence for coding the nanometer antibody, an expression carrier and a host cell of the nanometer antibody, and a method for producing the nanometer antibody. The nanometer antibody for resisting the salmonella enteritidis has the advantages that the size is small; the expression efficiency is high; the dissolution performance is good; the stability is high, and the like. The invention also provides the application of the nanometer antibody for resisting the salmonella enteritidis.

The invention relates to the field of food microorganisms, in particular to lactobacillus kefiranofaciens CICC 6278 and application of the lactobacillus kefiranofaciens to Xinjiang characteristic chili fermentation. Nitrite can be efficiently and quickly degraded by the lactobacillus kefiranofaciens, the lactobacillus kefiranofaciens is low in biogenic amine production, and pathogenic microorganisms can be inhibited by the lactobacillus kefiranofaciens. A bacterial strain of the lactobacillus kefiranofaciens is preserved in the China General Microbiological Culture Collection Center, and a preservation number of the bacterial strain is CGMCC 14017. The lactobacillus kefiranofaciens and the application have the advantages that the nitrite degradation rate can reach 94.8% if the bacterial strain is cultured in improved MRS liquid culture media for 24 h, and the nitrite can be completely degraded if the bacterial strain is cultured for 36 h; the bacterial strain comprises negative amino acid decarboxylase, and the pathogenic microorganisms including Escherichia coli, staphylococcus aureus, listeria monocytogenes and salmonella can be inhibited; the bacterial strain can be used for Xinjiang characteristic chili fermentation, the content of nitrite in obtained fermented chili is 1.06 mg / kg, the content of biogenic amine in the fermented chili is 5.62 mg / kg, and accordingly the edible safety of the fermented chili can be improved.

A vaccine for the prevention of anthrax, including a live, attenuated Salmonella and at least one nucleotide sequence encoding anthrax protective antigen (PA) or a fragment thereof and a nonlethal mutated form of anthrax lethal factor (LF) or a fragment thereof. In another implementation, the vaccine is constituted for the prevention of anthrax and at least one additional pathogen, as including a live, attenuated Salmonella and at least one nucleotide sequence encoding at least a fragment of a nonlethal mutated form of anthrax lethal factor (LF) and at least one nucleotide sequence encoding at least a fragment of an antigen of an additional pathogen. Vaccines of such types can be administered to stimulate antibody response in a subject, whereby the antibody response confers immunity to the subject.

The invention discloses a salmonella bacteriophage nano magnetic bead conjugate and its enrichment and separation kit. The conjugate is a complex of a salmonella bacteriophage coupled with a carboxylmagnetic bead, wherein the salmonella bacteriophage is Salmonella Typhimurium bacteriophage LPST10, and a preservation number is CCTCC NO: M 2016473. The bacteriophage of the Salmonella bacteriophagenano magnetic bead conjugate of the invention can specifically recognize and infect bacteria, and has the characteristics of good specificity and small molecular weight. The kit is capable of rapidlyand sensitively separating and enriching salmonella.

The invention provides a compound preparation for preventing necrotic enteritis of broilers and application of the compound preparation. Different bacterial strains and carriers are compounded with one another to obtain the compound preparation. The compound preparation comprises lactobacillus salivarius, garlic extract and folium mori . The content of butyric acid bacteria in the butyric acid bacteria, is not lower than 2*10<9> CFU / g, a preservation number of the lactobacillus salivarius is CGMCC No.11386, and the content of the lactobacillus salivarius with is not lower than 5*10<9> CFU / g. The compound preparation and the application have the advantages that obvious effects of inhibiting avian Escherichia coli, clostridium perfringens, salmonella and the like can be realized by the compound preparation, the compound preparation can be applied to raising the broilers, accordingly, the necrotic enteritis morbidity and mortality due to the clostridium perfringens can be obviously reduced, and growth of the broilers can be promoted.

The invention discloses sow perfusion fluid containing callicarpa nudiflora as well as a preparation method and application of the sow perfusion fluid. The sow perfusion fluid can achieve effects of promoting afterbirth and lochial discharge of sows after delivery, accelerating uterine recovery, preventing and treating sow hysteritis caused by infection of pathogenic bacteria (such as staphylococcus aureus, Escherichia coli, salmonella and pseudomonas aeruginosa), treating endometritis, promoting postpartum recovery and ensuring reproductive capacity of the sows, has lasting drug efficacy, anddoes not have irritation, drug residue or other harm to uterine mucosa of the sows.

The invention provides a bacteriocin produced by Bacillus coagulans and an application thereof, which belong to the field of microorganisms, the bacteriocin is extracted from of Bacillus coagulans with a preservation number of CGMCC NO. 9951, and can tolerate simulated gastric acid and simulate bile salt environment; has significant probioticity and can effectively inhibit growth and reproductionof Escherichia coli, Pastevula mulfocida, salmonella typhi, Salmonella, Shigella dysenteriae, Vibrio alginolyticus, Aeromonas hydrophila, and vibrio parahaemolyticus; and the bacteriocin has good stability and good antibacterial effect. The bacteriocin and bacteriocin-producing Bacillus coagulans can be used in the fields of prevention and control of poultry and aquatic diseases.

The invention discloses a double-antibody sandwich method for detecting beta-glucuronidase in escherichia coli cells of food, belonging to the technical field of immunoassay. The double-antibody sandwich method provided by the invention comprises the following steps of carrying out immune on seven-week BALB / c mice by using escherichia coli beta-glucuronidase (EC3.2.1.31), and fusing and screening to obtain ten monoclonal antibodies, respectively marking HRP and carrying out pairing; and establishing an sandwich ELISA analytical method by utilizing CGMCC No.7209 as a coating antibody, utilizing a CGMCC No.7211 as an enzyme labelled antibody, and utilizing the recombination expressive escherichia coli beta-glucuronidase as a standard substance. According to the double-antibody sandwich method provided by the invention, the beta-glucuronidase in escherichia coli ATCC 25922 detection cells is cracked, the escherichia coli is detected, and the LOD is 3.27*10^4cfu / mL; according to the method, the monoclonal antibody of a landmark of the escherichia coli, namely beta-glucuronidase, is prepared, the double-antibody sandwich method for detecting the escherichia coli is established, the method and salmonella, E.coli, enterobacter sakazakii, staphylococcus aureus and listeria monocytogenes do not have cross reaction, and the new detection method is provided for detecting the escherichia coli in food.

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

The invention discloses a culture medium for enriching salmonella, shigella and staphylococcus aureus in a composite way and a preparation method thereof. The culture medium comprises 13-16 parts of tryptone, 4-7 parts of soy peptone, 2-3 parts of monosodium orthophosphate, 2-3 parts of glucose, 1,000 parts of distilled water, 0.07-0.13 part of bile salt, 25-40 parts of sodium chloride, 0.4-1 part of lithium chloride, 1.5-3.5 parts of mannite and 0.0002-0.0004 part of potassium tellurite, and the pH of the culture medium is 7.1-7.3. The culture medium can be used for restraining the growth of other pathogenic microorganisms while enriching three target pathogenic bacteria simultaneously, can be directly applied to separate culturing of target bacteria and biological assay experiments, and can be directly applied to a detection technology for a plurality of pathogenic bacteria based on one detection platform such as multiple PCRs (Polymerase Chain Reactions) and the like for making a diagnosis report.

The invention discloses a method for inducing and forming Salmonella in VBNC state. Under ultrasonic treatment at 190-570W for 5-25min, it was detected whether Salmonella was induced to enter the VBNC state. The invention expands the understanding of Salmonella in VBNC state, and provides theoretical basis and technical support for better application of ultrasonic heating technology to food processing, microbial control and food safety assurance.

The invention discloses a construction method of a specificity salmonella H:z29 diagnostic serum engineering strain. The construction method is characterized in that a modern molecular biological technology is used for transforming a conventional multi-clone antibody preparation system, and surface flagellar antigen synthetic gene deleted host bacteria are constructed; surface flagellar antigen synthetic genes of different pathogenic microorganisms are cloned to carriers suitable for expression, and the carriers are transferred to the antigen gene deleted host bacteria so as to obtain a series of engineering strains having the same genetic background and containing different pathogenic microorganism specificity surface flagellar antigen genes. The technology system can be used for constructing a special antibody library comprising a plurality of salmonellae. The antibody library can be used for supplementing existing anti-serum types and meanwhile can provide basis for the application of other immunology technologies.

The invention discloses a pathogen multiplex PCR (polymerase chain reaction) detection kit for chicken common bacterial diseases. The kit comprises a PCR reaction solution, specific primers for Pasteurella multocida, Salmonella, Escherichia coli O157:H7 and Staphylococcus aureus, positive controls (standard substances of Pasteurella multocida, Salmonella, Escherichia coli O157:H7 and Staphylococcus aureus, or positive plasmids of Pasteurella multocida KMT1 gene, Salmonella invA gene, Escherichia coli O157:H7 rfbE gene and Staphylococcus aureus nuc gene), a negative control (sterile enzyme-free double distilled water), and a DNA marker. The quadruplex PCR is utilized to quickly, accurately and synchronously detect the four pathogenic bacteria; the kit has the advantages of high sensitivity and high specificity; the lower detection limit reaches 0.01ng / mu L; and the accuracy is up to 92.6%.

The invention discloses a double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) method for detecting listeria in food on the basis of monoclonal antibodies, belonging to the technical field of immunoassay. The method comprises the following steps: immunizing 8-week BALB mouse with prokaryotically expressed listeria monocytogenes P60 protein, and obtaining 15 strains of listeria specific monoclonal antibodies by immunization, fusion and multiple screening; respectively marking horse radish peroxidase HRP, and carrying out pairwise coupling by taking the listeria monocytogenes CMCC54003 as targets. The sandwich ELISA method which is established by screening different paired cross reactions and taking 2G7 as a coating antibody and 2H8-HRP as an enzyme labelled antibody has cross reactions to listeria including the listeria monocytogenes, and has no cross reactions to tested staphylococcus aureus, escherichia coli, escherichia coli O157, salmonella, enterobacter sakazakii, campylobacter jejuni and campylobacter coli. According to the method disclosed by the invention, the monoclonal antibodies having listeria specificity are prepared and the double-antibody sandwich ELISA method is established, and the method provides a technical means for specific rapid detection of the listeria in food.

The invention discloses a real-time fluorescence PCR based method for rapid screening of pathogenic microorganisms in cucumber. The method combines the traditional bacterial culture method, uses water-boiling method to extract salmonella, staphylococcus aureus, Escherichia coli O157:H7 and Listeria monocytogenes in selectively enriched cucumber, and applies real-time fluorescence PCR technology to screen the four food-borne pathogenic bacteria quickly. The DNA extraction technique adopted by the invention can complete the whole process of sample DNA extraction, and the real-time fluorescence PCR technique can whole process identification of a sample within 90min, therefore the method provided by the invention has the advantages of high efficiency, quickness, real-timeness, simple operation and the like, and provides technical support for rapid screening of food-borne pathogenic bacteria in fresh fruit and vegetable cucumber.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC4) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6786. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect of promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect on effectively protecting the SPF chickens.

The invention discloses a composite enrichment medium of salmonella, shigella and staphylococcus aureus. The composite enrichment medium comprises the following components in parts by weight: 5-15 parts of tryptone, 2.5-7.5 parts of a yeast extract, 5-15 parts of sodium chloride, 1-3 parts of glucose, 1-3 parts of sodium citrate, 1-2.5 parts of magnesium sulfate, 1-2.5 parts of L-phenylalanine, 1-3 parts of mannitol, 1-2.5 parts of sodium pyruvate and 1000 parts of distilled water. In the invention, the three types of the bacteria are the main detection parts of the national food hygienic standard pathogenic bacteria and can form proliferation advantage during the culture process of the composite enrichment medium, and the bacterial enrichment speed is faster and relatively consistent; after bacterial enrichment is carried out for 4 hours, separation culture, a biochemical authentication experiment and PCR authentication are performed; and after bacterial enrichment is carried is carried out for 18-24h, bacteria in an active non-culture status can make an accurate detection report, thus achieving wide application range and large usage amount, effectively lowering cost, simplifyingdetection procedures, enhancing working efficiency and being fast and accurate.