33 results about "Salmonella sps" patented technology

There are provided novel synthetic stool preparations comprising bacteria isolated from a fecal sample from a healthy donor. The synthetic stool preparations are used for treating disorders of the gastrointestinal tract, including dysbiosis, Clostridium difficile infection and recurrent Clostridium difficile infection, prevention of recurrence of Clostridium difficile infection, treatment of Crohn's disease, ulcerative colitis, irritable bowel syndrome, inflammatory bowel disease, and diverticular disease, and treatment of food poisoning such as salmonella. Methods of preparation and methods of use of the synthetic stool preparations are also provided.

A vaccine for the prevention of anthrax, including a live, attenuated Salmonella and at least one nucleotide sequence encoding anthrax protective antigen (PA) or a fragment thereof and a nonlethal mutated form of anthrax lethal factor (LF) or a fragment thereof. In another implementation, the vaccine is constituted for the prevention of anthrax and at least one additional pathogen, as including a live, attenuated Salmonella and at least one nucleotide sequence encoding at least a fragment of a nonlethal mutated form of anthrax lethal factor (LF) and at least one nucleotide sequence encoding at least a fragment of an antigen of an additional pathogen. Vaccines of such types can be administered to stimulate antibody response in a subject, whereby the antibody response confers immunity to the subject.

The present invention is drawn to multivalent Salmonella enterica serovar conjugate vaccines comprising conjugates of S. Typhimurium, S. Enteritidis, S. Choleraesuis, S. Typhi, S. Paratyphi A and optionally S. Paratyphi B, wherein the conjugates comprise a hapten antigen and a carrier antigen, wherein at least one of the hapten antigens or carrier antigens is characteristic of the Salmonella enterica serovar. The present invention also provides Salmonella enterica serovar reagent strains to produce the multivalent conjugate vaccines and attenuated Salmonella enterica serovars for use as vaccines.

The invention discloses a culture medium for enriching salmonella, shigella and staphylococcus aureus in a composite way and a preparation method thereof. The culture medium comprises 13-16 parts of tryptone, 4-7 parts of soy peptone, 2-3 parts of monosodium orthophosphate, 2-3 parts of glucose, 1,000 parts of distilled water, 0.07-0.13 part of bile salt, 25-40 parts of sodium chloride, 0.4-1 part of lithium chloride, 1.5-3.5 parts of mannite and 0.0002-0.0004 part of potassium tellurite, and the pH of the culture medium is 7.1-7.3. The culture medium can be used for restraining the growth of other pathogenic microorganisms while enriching three target pathogenic bacteria simultaneously, can be directly applied to separate culturing of target bacteria and biological assay experiments, and can be directly applied to a detection technology for a plurality of pathogenic bacteria based on one detection platform such as multiple PCRs (Polymerase Chain Reactions) and the like for making a diagnosis report.

The invention discloses a method for inducing and forming Salmonella in VBNC state. Under ultrasonic treatment at 190-570W for 5-25min, it was detected whether Salmonella was induced to enter the VBNC state. The invention expands the understanding of Salmonella in VBNC state, and provides theoretical basis and technical support for better application of ultrasonic heating technology to food processing, microbial control and food safety assurance.

The invention discloses a pathogen multiplex PCR (polymerase chain reaction) detection kit for chicken common bacterial diseases. The kit comprises a PCR reaction solution, specific primers for Pasteurella multocida, Salmonella, Escherichia coli O157:H7 and Staphylococcus aureus, positive controls (standard substances of Pasteurella multocida, Salmonella, Escherichia coli O157:H7 and Staphylococcus aureus, or positive plasmids of Pasteurella multocida KMT1 gene, Salmonella invA gene, Escherichia coli O157:H7 rfbE gene and Staphylococcus aureus nuc gene), a negative control (sterile enzyme-free double distilled water), and a DNA marker. The quadruplex PCR is utilized to quickly, accurately and synchronously detect the four pathogenic bacteria; the kit has the advantages of high sensitivity and high specificity; the lower detection limit reaches 0.01ng / mu L; and the accuracy is up to 92.6%.

The invention discloses a double-antibody sandwich ELISA (enzyme-linked immuno sorbent assay) method for detecting listeria in food on the basis of monoclonal antibodies, belonging to the technical field of immunoassay. The method comprises the following steps: immunizing 8-week BALB mouse with prokaryotically expressed listeria monocytogenes P60 protein, and obtaining 15 strains of listeria specific monoclonal antibodies by immunization, fusion and multiple screening; respectively marking horse radish peroxidase HRP, and carrying out pairwise coupling by taking the listeria monocytogenes CMCC54003 as targets. The sandwich ELISA method which is established by screening different paired cross reactions and taking 2G7 as a coating antibody and 2H8-HRP as an enzyme labelled antibody has cross reactions to listeria including the listeria monocytogenes, and has no cross reactions to tested staphylococcus aureus, escherichia coli, escherichia coli O157, salmonella, enterobacter sakazakii, campylobacter jejuni and campylobacter coli. According to the method disclosed by the invention, the monoclonal antibodies having listeria specificity are prepared and the double-antibody sandwich ELISA method is established, and the method provides a technical means for specific rapid detection of the listeria in food.

The invention discloses a lactobacillus salivarius strain and application thereof. The lactobacillus salivarius strain (with number of HVRIC4) is obtained by carrying out bacteria reproduction and enrichment on wild chicken manure in an MRS liquid selective medium and carrying out isolated culture and inoculation and purification. The microbial collection number of the strain is CGMCC No.6786. Lactobacillus salivarius has stronger acid and bile salt resistance. Antibacterial experiments in vitro indicate that the strain has stronger antibacterial effects on Gram-negative escherichia coli, salmonella and Gram-positive staphylococcus, shows a broad-spectrum antibacterial effect, has the effect of promoting growth of SPF (specific pathogen free) chickens, can effectively resist infection with salmonella and avian infectious laryngotracheitis viruses when being fed to the SPF chickens and has the effect on effectively protecting the SPF chickens.

The invention discloses a multiple PCR detection kit for 11 intestinal pathogen nucleic acid and application of the detection kit. The detection kit comprises primers for amplifying 11 intestinal pathogens which include vibrio cholerae serotype O1, vibrio cholerae serotype O139, salmonella, Shigella, vibrio parahaemolyticus, Yersinia enterocolitica, enterophathogenic escherichia coli (EPEC), enteroinvasive escherichia coli (EIEC), enteroaggregative escherichia coli (EAEC), enterotoxigenic escherichia coli (ETEC) and escherichia coli O157:H7. The multiple PCR detection kit has the advantages that the kit is capable of performing multiple detection, high in sensitivity and fast and convenient to use; specific primer sequences are used to guarantee detecting result reliability; the detection method is simple to operate, time saving, labor saving, high in detection throughput, low in reagent consumable cost, capable of directly detecting the nucleic acid extracted from encephalitis pathogens, low in detection platform and staff technical level requirements and capable of being widely popularized in conventional detection.

The invention discloses a composite enrichment medium of salmonella, shigella and staphylococcus aureus. The composite enrichment medium comprises the following components in parts by weight: 5-15 parts of tryptone, 2.5-7.5 parts of a yeast extract, 5-15 parts of sodium chloride, 1-3 parts of glucose, 1-3 parts of sodium citrate, 1-2.5 parts of magnesium sulfate, 1-2.5 parts of L-phenylalanine, 1-3 parts of mannitol, 1-2.5 parts of sodium pyruvate and 1000 parts of distilled water. In the invention, the three types of the bacteria are the main detection parts of the national food hygienic standard pathogenic bacteria and can form proliferation advantage during the culture process of the composite enrichment medium, and the bacterial enrichment speed is faster and relatively consistent; after bacterial enrichment is carried out for 4 hours, separation culture, a biochemical authentication experiment and PCR authentication are performed; and after bacterial enrichment is carried is carried out for 18-24h, bacteria in an active non-culture status can make an accurate detection report, thus achieving wide application range and large usage amount, effectively lowering cost, simplifyingdetection procedures, enhancing working efficiency and being fast and accurate.