The invention discloses a method for separating and enriching sulfa
antibiotics in drinking water. The method comprises a step of adjusting the pH of a
water sample to 3 to 4 with acid and filtering to obtain a preprocessed
water sample, a step of sequentially activating an MCX
solid phase extraction column with
methanol and
ultrapure water and allowing sampled water to pass through the column, astep of
drying the MCX
solid phase extraction column under the protection of
nitrogen after the enrichment is completed, rinsing the column with
ultrapure water with pH of 3 to remove water-soluble interference impurities of the
water sample enriched on the MCX
solid phase extraction column, eluting with a
methanol solution containing 8-15%
ammonia water, slowly blowing an eluate with
nitrogen stream to be nearly dry, and adding an
aqueous solution re-dissolved residue containing
methanol to complete the separation and enrichment of the sulfa
antibiotics in the drinking water. The method of the invention has the advantages of simple operation, environmental friendliness and high
enrichment ratio, multiple drugs of the same type of
antibiotics and other drugs with similar physical and chemical properties to sulfonamides can be simultaneously enriched, the method is particularly suitable for the separation detection of trace sulfonamide antibiotics in a drinking
water body, and the accuracy is high.