Electrophoresis microchip with online ion enrichment device and detection method

Abstract

The invention discloses an electrophoresis microchip with an online ion enrichment device. The electrophoresis microchip comprises an electrophoresis microchip body and a sample injection pool arranged in the electrophoresis microchip body. The sample injection pool is communicated with a sample enrichment pool through ion enrichment flow channels. The sample enrichment pool is communicated with asample waste liquid pool through an ion sample injection flow channel. A buffer solution injection tank and a buffer solution waste tank are communicated through an ion separation flow channel. The ion sample injection flow channel and the ion separation flow channel are vertically arranged in a crossed manner and are internally communicated. The volume of the sample injection pool is larger thanthat of the sample enrichment pool, and the ion enrichment flow channels are located at the shortest distance between the sample injection pool and the sample enrichment pool. The invention also discloses a detection method using the electrophoresis microchip. A two-time enrichment structure and a horn mouth shape are innovatively adopted, the ion detection sensitivity of the electrophoresis microchip is greatly improved, and ions can be rapidly enriched, separated and detected on the electrophoresis microchip.

Description

technical field [0001] The invention relates to the field of microfluidic chip electrophoresis, in particular to an electrophoresis microchip with an ion online enrichment device and a detection method. [0002] technical background [0003] Ion detection technology plays a vital role and significance in many fields such as clinical medicine, water quality monitoring, soil survey, food and drug quality management. as K in soil + 、Na + , Li + , NH 4 + , Ca 2+ , Mg 2+ Plasma is an important indicator of soil quality survey; NH in water 4 + 、PO 4 + When the plasma content is too much, it will cause the eutrophication of the water body, the algae in the water body will multiply rapidly, and other water body organisms will die in large numbers, thus destroying the ecological balance; while the Cd in the water body and soil 2+ , Pb 2+ Such heavy metal ions will gradually accumulate in the food chain and eventually be brought into the human body to cause diseases. Due t...

AI Technical Summary

Problems solved by technology

Among them, the isokinetic electrophoresis enrichment technology and sweeping technology, although the enrichment efficiency is high and the enrichment multiple is large, but the chip production is difficult, the cost is high, and the operation is cumbersome.

Although the field amplification enrichment technology has the advantages of no need for other equipment, low cost and simple operation, it also has the disadvantages of low enrichment efficiency and small enrichment multiples.

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