466results about How to "High enrichment efficiency" patented technology

The invention relates to a preparation method of covalent organic framework composite microspheres with core-shell structures. The method comprises the following steps: dissolving silicon dioxide microspheres, a first construction element and a second construction element in an organic solvent; and after adding a catalyst, rapidly synthesizing into the composite microspheres with core-shell structures at a certain temperature. In a preparation process, reaction conditions are gentle, the method is simple, and the yield is high; the prepared covalent organic framework composite microspheres have the advantages of good core-shell morphology, high specific surface area, ordered pore structures, good mechanical stability, thermal stability, chemical stability and the like, if the microspheres are used as a chromatographic stationary phase, the chromatographic mass transfer resistance can be reduced effectively, the theoretical plate height is improved, and finally, column efficiency and degree of separation are improved; and if the microspheres are used as solid-phase extracting filler, the enrichment effect can be improved remarkably. The covalent organic framework composite microspheres with core-shell structures have good application prospects in the aspect of separation and enrichment of small organic molecules.

The invention relates to a magnetic solid-phase extraction agent based on a covalent organic framework material and a preparation method and application thereof. The magnetic solid-phase extraction agent comprises a ferriferous oxide magnetic nanoparticle core, a polydopamine layer and a covalent organic framework material COF-5 layer, and the polydopamine layer and the covalent organic framework material COF-5 layer are sequentially coated outside the core. A method includes: adding functionalized magnetic nanoparticles into a to-be-treated glycoprotein sample, sufficiently stirring for extraction, using a magnet to separate the magnetic nanoparticles from a solution, adding elution liquid to elute off the glycoprotein from the magnetic nanoparticles, and measuring. The covalent organic framework material can selectively enrich glycoprotein and effectively separate the glycoprotein from impurities, so that the method has good extraction effect on the glycoprotein. The solid-phase extraction method is simple in operation step, short in time, high in recycling rate, low in organic solvent consumption and suitable for enrichment and measurement of the glycoprotein in complex biological samples like plasma and urine.

The photosynthetic bacterium immobilizing process includes preparing zeolite powder of 120-200 mesh, adding sodium alginate solution of 500-1000 mpa.s viscosity and concentrated photosynthetic bacterium liquid in the weight ratio of 1:0.03 to 0.05:0.2-0.3, mixing through stirring, pelletizing, hardening in 20-40 g / L concentration CaCl2 solution, and final clear water rinsing to obtain the product. The process has great photosynthetic bacterium immobilizing amount, high immobilizing efficiency, and high activity of the immobilized photosynthetic bacterium, and the product can deposit fast to water bottom for degrading organic pollutant and purifying water without loss.

The invention provides a full online detection multi-dimensional liquid chromatogram separation system based on a same detector. The full online detection multi-dimensional liquid chromatogram separation system comprises a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid diluent pump, a gradient mixer A, a gradient mixer B, an injection valve, an enriching column array A, an enriching column array B, a fraction collector, a liquid chromatography separation column array, a detector, a two-position ten-wayvalve and a connecting pipeline; the conversion of the previous-dimension separation state and the current-dimension separation state is realized through the switching of the two-position ten-way valve, so that three dimensions or more than three dimensions of chromatographic separation is realized. Each dimension of chromatographic separation column is selected through the liquid chromatographyseparation column array, full online monitoring and control for multi-dimensional chromatographic separation are realized based on the same gradient elute system and the same detector, and cleanlinessdegree controllability for enriching columns and separation columns is realized. Each dimension of separation is connected through the enriching columns, and the enrichment or gathering of compoundsis assisted by using the diluent pump. According to the system, efficient separation for complex system samples with high separation difficulty is realized by selecting the combinations of different chromatographic stationary phases and moving phases.

The invention relates to a biochip for enriching and detecting circulating tumor cells and preparation method thereof. The invention comprises the following steps: placing a clean substrate on flame, obtaining a cigarette ash layer composed of nano cigarette ash particles on surface of the substrate by deposition, using the nano cigarette ash particle as template, and deposing a layer of silica housing layer on external surface of nano cigarette ash particles by chemical vapor deposition method; removing nano cigarette ash particles in the silica housing layer by high temperature calcining, and obtaining a silicon dioxide layer composed of nano silica particles with micrometer thickness; finally, modifying an anti-EpCam antibody which can specifically identify tumor cells on the surface of the silicon dioxide layer, thereby obtaining a biochip for enriching and detecting circulating tumor cells. The biochip can enrich circulating tumor cells with high efficiency and high sensitivity, and the product has high transparency under water and can be used for detecting real time captured circulating tumor cells.

The invention discloses a magnetic cadmium ion-imprinted polymer and a preparation method thereof. The magnetic cadmium ion-imprinted polymer is prepared by taking vinyl-modified Fe3O4@SiO2 as a magnetic carrier, Cd<2+> as a template, a combination of any two of methacrylic acid, acrylamide and vinyl pyridine as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linking agent, and azodiisobutyronitrile (AIBN) as an initiator. The magnetic cadmium ion-imprinted polymer is capable of carrying out enrichment and separation on trace cadmium in an agricultural product sample, and is high in detection sensitivity and accuracy; furthermore, the magnetic cadmium ion-imprinted polymer has the advantages of being simple in preparation method and low in cost and operation requirement, having higher selectivity for template ions, and the like; the product also can be rapidly separated in presence of an external magnetic field, so that the tedious operation such as centrifugation and filtration are avoided, and enrichment and separation efficiencies are improved; the magnetic cadmium ion-imprinted polymer has a wider and good application prospect in the field of detection of food and agricultural product samples.

The invention discloses a film sample introduction enriching device in an online gas analysis mass spectrometer, the film sample introduction enriching device comprises a film, a vacuum difference cavity body, a circulation pump, a fine tuning vacuum control valve, a tubule, a capillary, a sample inlet tube, a sample feeding valve, a sample feeding cavity, an end cover, a mechanical pump and a difference extraction opening. The film sample introduction enriching device has the advantages of good mobility, being capable of rapidly enriching samples of organic contaminants in air, realizing direct sample introduction and online enriching of samples, shortening sample responding time, dynamic balance status of the vacuum of the vacuum difference cavity body and constant degree of vacuum in the mass spectrometer during the analysis process, which is helpful to quantitative analysis, effectively reduces the adsorption and memory effect of the samples at the film surface and prolongs the service life of the film, and the enriching efficiency of the film sample introduction system can be controlled according to the test requirements.

The invention discloses a mass spectrum rapid enrichment-thermal analysis membrane sample introduction apparatus and an application. The apparatus comprises an enclosed cavity, and a heating wire whose outer surface is sleeved by a tubular enrichment membrane passes through the center of the cavity; a sample inlet and a sample outlet are arranged on the wall of the cavity, the sample inlet is connected with a sample pool through a sample induction pipe, the sample outlet is connected with a tail gas pipe or a waste liquid pool through a sample discharge pipe, and a sampling device is arranged on the sample introduction pipe or the sample discharge pipe; the wall of the cavity is provided with a sweeping gas inlet and a sweeping gas outlet, the sweeping gas inlet is designed in a tee structure, one end communicates with an external gas source, one end is empty, and the other end is directly connected with the wall of the cavity; the enclosed cavity is connected with a sample introduction capillary of a mass spectrometer through a valve; and the sweeping gas outlet is connected with the tail gas pipe or the waste liquid pool through the sample discharge pipe. The apparatus provided by the invention is combined with a mass spectrometer for application, sample preprocessing is unnecessary, and the apparatus is suitable for quasi online mass spectrometry of semi-volatile organic compounds (SVOCs) in an environment.

The invention discloses a kit for extracting and enriching free DNAs. The kit comprises a sample lysate, a binding solution, a primary washing fluid, a secondary washing fluid, an eluent and nano magnetic beads. The sample lysate comprises nuclease-free water, a reagent A and a reagent B, the reagent A is at least one of guanidine isothiocyanate, guanidine hydrochloride, sodium iodide and sodium perchlorate, and the reagent B is at least one of Tween20, Triton-X100, SDS, NP40 and a polyoxyethylene type nonionic surfactant. The binding solution comprises a reagent C in addition to the components of the sample lysate, and the reagent C is at least one of isopropanol and absolute ethanol. The primary washing fluid comprises the same components as the binding solution. The secondary washing fluid is the nuclease-free water, a sodium chloride solution with the molar concentration of 0.1-1M, a Tris buffer solution or a TE buffer solution. The eluent is the nuclease-free water, the Tris buffer solution or the TE buffer solution. The invention further discloses an extraction method for the free DNAs. The technical problem that organic solvents in the prior art have a certain toxicity is solved.

The invention relates to a boron carrying agent for integrating tumor diagnosis and treatment. The boron carrying agent is tyrosine boron trifluoride (FBY) obtained by replacing a carboxyl group witha boron trifluoride group on the basis of tyrosine. FBY can be used for boron neutron capture therapy (BNCT) for treating malignant tumors; radioactive <18>F-labeled FBY can also be used for positronemission tomography (PET) imaging diagnosis. <18F>-FBY can simultaneously achieve imaging diagnosis and precise treatment of cancers.

The invention relates to a method for preparing chloramphenicol affinant column and its usage, which adopts protein A -Sepharose 4 fast flow as phase carrier, the protein A of the carrier is coupled with the chloramphenicol antibody; the coupled chloramphenicol antibody is bonded with the fixed phase chemistry; the affinant column can suit for many stroma; it uses protein A so that IgG can be connected with it with high desirability and large load capacity of the chloramphenicol column.

The invention discloses an optimizing method of enriched ammonia-oxidizing bacteria in an activated sludge population structure, belongs to the technical field of enhanced biological sewage denitrification processing, aims at solving the practical problems of trenchant running condition requirements, long cultivation time of enriched ammonia-oxidizing bacteria and high running energy consumption in current denitrifying techniques with short-course nitration and denitrification, and develops the optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure according to the growth characteristics of ammonia-oxidizing bacteria and nitrite-oxidizing bacteria in an activated sludge processing system. The optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure is mainly characterized in that a nitration reaction process is controlled with parameters of pH value and DO, aeration is halted before ammonia peak valley appears, ammonia-oxidizing bacteria is completely proliferated, proliferation chance of NOB is deprived, and nitrite-oxidizing bacteria is eliminated from the system by controlling sludge age, thus achieving the goal for the quick start of short-course nitration and denitrification. A bisection method is utilized in the operating process so as to determine the optimal time for advancing aeration halt. Furthermore, the optimizing method of enriched ammonia-oxidizing bacteria of the activated sludge population structure has the advantages of short reaction period, high enrichment efficiency, simple operation and the like.

The invention discloses a preparation method for a novel solid-phase micro-extraction head based on a single-stranded DNA aptamer modified graphene oxide coating. The preparation method comprises the following steps: (1) subjecting a quartz fiber to silanization; (2) coating a porous polymer solution on the surface of the quartz fiber; (3) repeatedly coating a porous polymer coating; (4) carrying out silanization; (5) intercalating the silanized porous polymer coating quartz fiber into a mixed solution of a PBS buffer solution, a catalyst EDC solution, an NHS solution and an aqueous graphene oxide solution; (6) repeating step (4) and step (5) a plurality of times on the same quartz fiber; and (7) taking out the graphene oxide / porous polymer coating quartz fiber and intercalating the graphene oxide / porous polymer coating quartz fiber into a mixed solution of the PBS buffer solution, the EDC solution, the NHS solution and an aptamer solution. The preparation method provided by the invention overcomes the disadvantages of a low immobilization rate, easy shedding, instability and the like of conventional coating methods, substantially increases the immobilization rate of an aptamer and improves enrichment efficiency of SPME.

A laminated magnet array architecture device for gathering oxygen of air through magnetism and a method thereof belongs to gas separation technique field. The device comprises: a laminated magnet array, a magnetic field space, fluid damping layer on opened border of the magnetic field space, a air source, a buffer, a air delivery pipe, an inlet transition section, an outlet transition section, oxygen enriched air delivery pipe, valves, nitrogen enriched air delivery pipe, a shell and an electric heater of oxygen enriched air outlet. The method is that air is injected into a magnetic field space with an opened border and a gradient magnetic field which gradient direction points to inside on the border, when air outflows from the opened border, oxygen molecules of air are constrained in the magnetic field by that the gradient magnetic field acts force on oxygen molecules. The invention has advantages of simple structure, large amount of treated air, low energy consumption, long service life and wide adaptability.

The invention discloses a mobile adsorption concentration-thermal desorption device and an application method thereof. The device comprises a control system, a gas circuit part and a mobile cold trap consisting of a heating module, a refrigerating module and a mobile mechanism. The method comprises the steps of control system starting, operation of the refrigerating module, sample collection, operation of the heating module, sample desorption, pipeline cleaning, and the like. The device has the advantages that a refrigerating fin is easy to damage after long-time overheating, and the gas adsorption and gas enrichment efficiency is effectively improved. In comparison with the prior art, the device shortens the adsorption and analysis time, reduces the time consumption, lowers the cost and improves the concentration efficiency.

The invention discloses a magnetic nanoparticle and an immunomagnetic nanoparticle with a cell-like structure as well as a preparation method and application thereof. The magnetic nanoparticle with the cell-like structure uses a superparamagnetic Fe3O4 nanoparticle as a core and is wrapped with a white cell membrane on the surface, so that the magnetic nanoparticle has excellent magnetic responseperformance, furthermore, the white cell membrane wrapping on the surface of the magnetic nanoparticle is homologous with white cells, thereby effectively weakening non-specific adsorption to the white cell; an antibody is modified onto the white cell membrane wrapping on the surface of the magnetic nanoparticle through mediating of lipid molecules-polyethylene glycol-biotin molecules and avidin,so as to obtain the immunomagnetic nanoparticle with the cell-like structure. CTCs cells can be enriched and separated with high efficiency and high purity.

The invention relates to a method for enrichment of vanadium in vanadium-containing stone coal and V-concentrating phase regulation. The method includes: taking stone coal vanadium ore as the raw material, using Fe2O3 and carbon powder as the additives; carrying out crushing and mixing to obtain pellets, performing roasting at a temperature of 900-1100DEG C so as to obtain a V-concentrating phase composed of Fe, V and O in stone coal. The method is characterized in that: (1) vanadium is enriched into the V-concentrating phase composed of Fe, V and O by a high temperature chemical process, and compared with the conventional physical beneficiation method, the enrichment efficiency is higher; (2) the adding amount of carbon powder can be adjusted according to the carbon content of stone coal, the adaptability is good, and the treatment scope is wider; and (3) by changing the roasting conditions, V-concentrating phase regulation can be realized. The method provided by the invention not only can the V-concentrating phase with high vanadium content and particle size suitable for subsequent separation, but also can control the V-concentrating phase, has the advantages of wide application range and high enrichment efficiency, and is suitable for application in the technical field of vanadium preconcentration in vanadium-containing stone coal.

The invention relates to a recovery method for lithium, and more particularly to an electrochemical recovery method of lithium in cathode materials of waste lithium batteries. The method comprises the following steps: dividing an electrolytic cell into two areas: an anode chamber and a cathode chamber by using a monovalent cation selective permeable membrane; taking the cathode materials of the waste lithium batteries as an anode, and taking at least one of a lithium salt solution, a divalent cationic electrolyte solution and a trivalent cationic electrolyte solution as an anode chamber electrolytic solution; taking an inert electrode material as a cathode, and taking a lithium salt solution as a cathode chamber electrolyte solution; applying external potential, enabling the lithium in the electrode materials of the waste lithium batteries to form li-ion dissolved in the anode chamber electrolyte solution, enabling the li-ion to enter into the cathode chamber for enrichment through monovalent cation selective permeable membrane, so as to obtain a lithium-rich solution. The method has the advantages of being simple and effective, low in cost and high in lithium enrichment efficiency, the recovery rate of lithium is up to 95% or more, the purity of lithium liquid is high, and continuous recovery of lithium in electrode materials of waste lithium batteries can be realized.

The invention discloses a method for collecting dioxin from incineration flue gas of household garbage. The method comprises the following steps: the incineration flue gas of the household garbage sequentially passes through a first-stage double-pipe heat exchanger, a filter cylinder and at least one stage after-treatment double-pipe heat exchanger; the flue gas flowing out of the last stage after-treatment double-pipe heat exchanger sequentially passes through a n-hexane gas-washing bottle, a buffer bottle, a glass filling tube, a toluene gas-washing bottle and a buffer bottle which are placed in an ice-water mixture; the inner wall of a heat exchange sleeve of the first-stage double-pipe heat exchanger is washed with toluene solution in the gas-washing bottle, and the toluene solution is collected after washing; and a collection bottle is arranged at the lower part of the first-stage after-treatment double-pipe heat exchanger, condensed water containing a dioxin matter generated by condensation in the flue gas is received, and the filter cylinder containing smoke dust, the condensed water, resin and toluene for washing the inner wall of the heat exchange sleeve of the first-stage double-pipe heat exchanger are taken as final samples. By adopting the method, the enrichment efficiency of the dioxin is improved, and the dioxin in the incineration tail gas of the household garbage can monitored more accurately.

The invention discloses a dynamic headspace-cold focusing-GC (Gas Chromatography)-MS (Mass Spectrometer) tobacco headspace element analysis method comprising the following steps of: arranging a tobacco sample in a dynamic headspace instrument, heating the tobacco sample, blowing and sweeping the tobacco sample continuously, enabling the blown and swept tobacco headspace elements to enter into a gas chromatograph PTV (Programmed Temperature Vent) through a transmission pipeline to be condensed and enriched, separating the enriched tobacco elements through a gas chromatograph GC, and then enabling the separated tobacco elements to enter into the MS for carrying out qualitative and quantitative analysis.

The invention discloses a polymer brush graft boracic acid affinity separation material and a preparation method and application, which use a reversible-addition fragmentation chain transfer (RAFT) polymerization method, use amide compounds with phenylboronic acid groups as monomers, wherein the amide compounds are polymerized on the surfaces of polyacrylic acids, silica gels, agarose and magnetic nanoparticles to obtain the polymer brush graft boracic acid affinity separation material. The polymer brush graft boracic acid affinity separation material has the advantages of high adsorption capacity, excellent selectivity and rapid mass transfer rate, can be used for preparation, separation and content analysis of biomolecules containing cis vicinal diols, and can greatly improve separation enrichment efficiency.

The present invention relates to a method for quickly enriching trace polypeptide or protein sample by using nano calcium carbonate particles in-situ polymerized polymethyl methacrylate (CaCo3-PMMA) as nano adsorbing agent and utilizing solid-free miniaturated matrix auxiliary laser analysis ion source mass spectrography to make direct analysis. The invented nano material not only can implement quick and high-effective enrichment of protein and polypeptide, but also has good compatibility with matrix auxiliary laser analysis ion source mass spectrography. Said invention can be extensively used in the field related to protein phylogeny.

The invention discloses a method for detecting phenolic antibacterial agents in textiles through the combined use of in-situ derivatization, dispersive liquid phase microextraction and gas chromatography / mass spectrometry. A technical program suitable for the simultaneous detection on five types of chlorinated phenols and o-phenylphenol in the textiles is established, a target compound is extracted by utilizing a dispersive liquid phase microextraction technology after being subjected to extraction and derivatization, and qualitative and quantitative analysis is carried out by using a gas chromatograph / mass spectrometer. The method can be applicable to the determination of the five types of chlorinated phenols and o-phenylphenol in the textiles such as clothes, fiber packing, yarns, clothing materials and household decoration products. The method is simple and rapid to operate, low in cost, environment-friendly and high in enrichment efficiency.

The present invention discloses a method for enriching phthalate in air through a graphene / ionic liquid composite material. According to the present invention, the material obtained by compounding graphene and a poly-1-allyl-3-ethylbenzimidazole hexafluorophosphate ion liquid is adopted as an adsorbent, phthalate in air is analyzed by a thermal desorption-gas chromatography-mass spectrometry combined detection means, and graphene oxide prepared by using a ball milling method is activated with CO2 to obtain the carbon material with characteristics of high specific surface and more pore channels so as to increase the enrichment efficiency; and the method has the following advantages that the use of a large number of the organic solvent is not required, the problems of solvent evaporation, cross-contamination and the like are avoided, the operation steps are simple, the selectivity on phthalate is good, the sensitivity is high, the adsorbent can be recycled, and the gas-state phthalate and the granular phthalate can be simultaneously enriched.

The invention relates to an application of a magnesium oxide microsphere in environmental water samples, and particularly a method of using a magnesium oxide microsphere as a solid phase extraction adsorbent for enrichment of polycyclic aromatic hydrocarbons in environmental water samples. A solid phase extraction empty tube is filled by a dry method; various experimental parameters of the solid phase extraction process (column equilibrium, sample application, leaching, elution) are optimized, including the type (methanol, isopropanol, acetone...) and the concentration (0-30%) of a modification agent, the type (dichloromethane, n-hexane, ethyl acetate...) of an elution solvent, the sample applying volume (10-250 mL), the sample applying flow rate (1.0-10.0 mL / min), so as to obtain an optimal condition; and finally the method is applied to the enrichment of polycyclic aromatic hydrocarbons in environmental water samples. The invention has the advantages of simple operations, low cost, environment-friendly performance, and high efficiency.

The invention provides a cancer monitoring-oriented biological coating for the specific whole blood capture of circulating tumor cells and a preparation method of the biological coating and belongs tothe field of biotechnology and clinics. According to the coating, polyglycidyl ether (PG) or polyethylene glycol (PEG) is adopted, the adhesion bionic chemical modification of mussels is used in combination, and therefore, the specific capture of the circulating tumor cells can be performed. According to the biological coating, and the preparation method thereof, the surface of a substrate is modified with a functionalized polymer; the adhesion of non-specific cells to other blood components is prevented; a specific antibody is fixed on the surface of the polymer; a to-be-detected sample (blood) is dropwise added to the surface of the coating; the synergistic effect of specific adsorption and non-specific adhesion resistance is achieved through the synergistic effect of the specific antibody for identifying the circulating tumor cells and the non-specific adhesion resistance polymer, so that the circulating tumor cells in the to-be-detected sample can be efficiently and accurately captured. With the method provided by the invention adopted, the circulating tumor cells can be efficiently captured. The method has the advantages of low cost, simple operation and extremely high sensitivity, and can be used for clinical detection.

The invention belongs to the field of biochemical analysis, and relates to a magnetic nano-material, a preparation method and a purpose of the magnetic nano-material for a method for enrichment palmitoyl-modified protein / peptide segment. The method employs a synthesized nano Fe3O4 magnetic core as a material magnetic center, 3-(2-pyridine dimercapto)propionic acid N-hydroxysuccinimide eater (SPDP) is used for coating the Fe3O4 magnetic core, and a Fe3O4-coated NH2-coated SPDP magnetic nano-material is prepared: the nano-material can selectively increase the efficiency of enrichment of palmitoyl-modified protein / peptide segment, and can be used for analyzing a practical biological sample. The magnetic nano-material has the advantages of good selectivity, high sensitivity, simple preparation, and rapid and convenient separation, and the enrichment method of the magnetic nano-material has the advantages of simple operation, time saving and economy, and high efficiency and sensitivity.

The invention relates to the technical field of aquatic product virus detection, and discloses an ssDNA aptamer for identifying micropterus salmoides iridovirus. The nucleotide sequence of the ssDNA aptamer is shown as SEQ ID NO: 1-3, and the aptamer has the advantages of high specificity, high affinity, zero cytotoxicity, easy modification and substitution of different parts of the aptamer, and easy preservation. A prediction secondary structure of the aptamer provided by the invention has a unique stem-loop structure, the structure is stable and the target binding capacity is strong, so thatthe aptamer is suitable for preparing a micropterus salmoides iridovirus detection probe and a drug carrier and is applied to separation and purification of drugs; and rapid and efficient identification detection or treatment of micropterus salmoides iridovirus is facilitated, and the aptamer has a good application prospect.