The invention relates to a high-efficiency breeding method of healthy seedlings of
cane, the method is proposed aiming at the problems that seed performance degenerates, yield is greatly reduced and the quality is reduced, which are caused by that the
cane is taken as asexually propagated
crop, propagation coefficient is low, the amount of seeds used for
cane implantation is large, and after the implantation of a plurality of years, the cane is easy to be infected with a
disease; improved type healthy seedlings of the cane can be cultured by nine steps of
seedling mild water
detoxication processing,
seedling sprouting,
in vitro peeling
inoculation, induction culture,
virus check, propagation culture, rootage culture, domesticated
seedling-refining and outdoor temporary planting; the method adopts the combination of mild water
detoxication and stem tip
detoxication with thorough detoxication and good effect; the direct peeling
inoculation of the explant does not need to be treated with disinfection
processing, has low
pollution rate and light
browning, and is easy to generate clustering buds by induction; the propagation coefficient is high, the speed is high, the period is short; the seed performance is table, the seedling is strong; the
virus detection is precise and accurate; the
survival rate of the outdoor temporary planting is high and is up to more than 90 percent; the production cost is low, the efficiency is high, and the method is suitable for industrialized production.