305 results about "Direct analysis" patented technology

This invention relates to a desorpton / ionization source operated under ambient conditions for direct analysis of solid or liquid samples on a surface. The source comprises of a laser desorption system and a UV / electrospray combined ionization system. The source is suitable for simultaneously ionizing samples with different polarity in a complex mixture. At the same time, the compact design of the source with multiple channels can maintain the level of local concentration of the analyte ions inside the source for higher efficiency of sample ionization and introduction.

An apparatus and method for direct analysis of continuous-flow liquid samples by desorption electrospray ionization-mass spectrometry (DESI-MS) including a sample stage that is adapted to receive a liquid sample and a nebulizing ionizer that is configured to generate a charged, nebulized solvent and thereby desorb at least a portion of the liquid sample from the sample stage.

The invention relates to a method and device for directly analyzing the generation of a sample and analyzing ions without pretreatment. The method comprises the following steps of: generating neutral molecules at atmospheric pressure; conveying the generated neutral molecules into a low-pressure area; and making the neutral molecules react with charged droplets sprayed from an electron spray needle point or photons emitted from a vacuum ultraviolet source in the low-pressure area for ionizing, wherein the ions generated in the process are converged and guided in an alternative electric field before being conveyed to a mass spectrograph or an ion migration spectrograph for further analyzing.

The present invention provides a device, system, and associated methods to actively or passively sample air by directing it onto the surface of a porous light-absorbing semiconductor, for example, a desorption / ionization on porous silicon (“DIOS”) chip. Upon adsorption of an analyte, the surface may be analyzed directly by laser desorption / ionization time-of-flight mass spectrometry. Because the process of laser desorption / ionization and subsequent mass detection does not require elevated temperatures, thermal degradation of analytes is avoided.

A method the direct analysis of an analyte in keratinized structures, e.g., hair and fingernails, which comprises preparing a mixture containing a low redox potential activator compound such as dithiothreitol or dithioerythritol, an enzyme suitable for the digestion of the keratin structure, a sample of the keratin structure and a biological detergent that aids the digestion of the keratinized structure at a relatively low pH, e.g., between about 6.2 and 8; permitting the enzyme to at least substantially digest the sample of keratin structure, and subjecting the digest solution to analysis, preferably by radioimmunoassay, to determine the identity and amount of analyte in the keratin structure sample. To accelerate the method, cupric sulfate may be added to the mixture after degradation of the keratin sample. The enzyme may be a peptidase, endopeptidase or proteinase, with papain, chymopapain, and proteinase K being preferred for use in the invention. The preferred biological detergents include betaine, sulfo-betaine, alkylglucosides and bile acids.

Devices and methods for thermally lysing of biological material, for example vegetative bacterial cells and bacterial spores, are provided. Hot solution methods for solubilizing bacterial spores are described. Systems for direct analysis are disclosed including thermal lysers coupled to sample preparation stations. Integrated systems capable of performing sample lysis, labeling and protein fingerprint analysis of biological material, for example, vegetative bacterial cells, bacterial spores and viruses are provided.

A system using tank corrosion sensors to provide for an overall assessment and monitoring of the electro-chemical corrosion and coatings condition in ships' tanks, and particularly in ships' seawater or compensated fuel tanks. The system includes reference half-cells mounted along a suspended cable and one instrumented sacrificial anode at the end of the cable to provide optimal sensing capability within a tank structure. The reference half-cells and the sacrificial anode measure a potential and current output, respectively. Together the measurements provide objective information that can be used to predict corrosion damage and coating deterioration occurring throughout the structure of the tank. The system may be used for an overall assessment and monitoring of the electrochemical corrosion and coatings condition. In a preferred embodiment, the measurements are stored in a datalogger that is optimally contained within an associated instrument housing. If used with other systems in other tanks, the system may be used to monitor the relative tank condition, trend tank condition changes over time, range tank behavior into three categories and provide a direct analysis methodology for making tank maintenance decisions.

An extraction and analysis device includes a microfluidic based collection system that extracts one or more different analytes from a fluid-based sample and an optical analysis system directly coupled to the collection system to perform optical analysis on the one or more collected analytes. The microfluidic based collection system includes microfluidic circuitry for directing a fluid based sample to a purification chip. Analytes collected within the purification chip can be either subsequently removed and analyzed or the analytes can be analyzed directly, while still within the purification chip, using the optical analysis system. The purification chip is preferably comprised of a plurality of pillars, the surface area of each pillar is coated with a specific capture chemistry. The specific capture chemistry is applied by derivitizing the pillars such that a ligand, such as a nucleic acid, an amptimer, or an antibody is attached to each pillar.

The invention discloses a vehicle-mounted dust-haze pollutant real-time on-line monitoring system and a vehicle-mounted dust-haze pollutant real-time on-line monitoring method. The monitoring system includes a PM2.5 online mass spectrum detection system, a VOCs online mass spectrum detection system and a VOCs online spectrum detection system which are arranged on a moving monitoring vehicle. The monitoring method includes the steps of performing on-line measurement to particulate matters in atmosphere through PM2.5 online time of flight mass spectrum to obtain number concentration of the particulate matters, spectrogram of particle size and online mass spectrum data, classifying the ingredients of the particle matters through an ART-2a method to obtain chemical ingredient data of single particle under different time resolutions and particle sizes, analyzing the source of the particulate matters through a mass spectrum direct analysis technology to determine the source of the particulate matters, directly performing online measurement to VOCs in atmosphere through the VOCs online time of flight mass spectrum to obtain the ingredient and concentration characters of the VOCs, and directly performing online measurement to the VOCs in atmosphere through VOCs online spectrum to obtain the ingredient and concentration characters of the VOCs.

The current invention involves a method and a device for generating and analyzing ions in order to analyze samples directly without sample preparation. The gaseous neutral molecules are desorbed under atmospheric pressure by a desorption method. The desorbed neutral molecules are then transferred into a low pressure region where they are post-ionized by a mist from an electrospray probe tip or by photons from a vacuum UV source. The generated ions are then focused in a time varying electric field in the low pressure chamber before they are transferred into a mass spectrometer or ion mobility spectrometer for further analysis.

A card or insert having a plurality of recesses for a sample preparation device, the card containing cast-in-place composite and / or non-filled structures which are useful as sorptive or reactive media or for size-based separations. Any particular card size or configuration can be used, and the inclusion of a large amount of adsorptive particles in polymer is achieved while still maintaining the membrane three dimensional structure. In a first preferred embodiment, the composite structures comprise particles entrapped within a porous polymeric substrate, and are cast in-place into a plurality of recesses in an insert for a multi-well sample preparation device, thereby providing an effective platform for high throughput micromass handling. With the appropriate selection of particle chemistry, virtually any separation or purification operation can be conducted in multiplicity, including selective bind / elute chromatography operations, on sample mass loads less than 1 microgram in volumes of a few microliters, as well as larger mass loads and volumes. Manufacturing flexibility and high throughput is achieved. The card can be configured for direct analysis of bound sample without elution.

A liquid chromatograph has switching valves as a flow passage switching mechanism and an automatic sampler as an injection section. The liquid chromatograph can switch, by switching the switching valves between a concentration flow passage for concentrating the sample injected by the automatic sample via a flow passage which connects the automatic sampler to the trapping column; a concentration analysis flow passage for separating and analyzing the concentrated sample on a per-composition basis which connects the trapping column to the analysis column; and a direct analysis flow passage by separating the sample on a per-composition basis which connects the automatic sampler to the analysis column.