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Method for preparing micro-flow chip

A microfluidic chip and cover slip technology, which is applied in chemical instruments and methods, laboratory containers, measuring devices, etc., can solve the problems of loss, lack of chips to analyze cells, and different expression levels, so as to achieve good activity and wide application Potential, the effect of easy operation

Active Publication Date: 2013-12-18
WUHAN YZY MEDICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, as an epithelial cell surface marker, EpCAM has different expression levels in different types of tumor cells, and its use is limited by the type of tumor cells.
In addition, the chemical modification steps for the substrate are cumbersome and time-consuming, which increases the success rate of uncertainty interference experiments
In addition, by manipulating the standing wave of the surface acoustic wave, the acoustic radiation force is used to directly manipulate the tumor cells for sorting and capture; the method of dielectrophoresis is used to sort the cells by using the difference in force of the dielectric particles in the uneven electric field. and capture, but these methods inevitably have adverse effects on cell viability
In addition, the chip obtained by using the current microfluidic chip preparation method lacks the ability to analyze cells on the chip after capturing tumor cells, and needs to be transferred to a centrifuge tube before subsequent experiments can be performed. This transfer process is difficult and will cause a lot of damage. cell loss

Method used

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Examples

Experimental program
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Embodiment 1

[0061] figure 1 It shows the preparation method of the microfluidic chip according to one embodiment of the present invention. First, enter the mask preparation operation S1100: use CorelDraw software to design two kinds of masks, and use a high-resolution laser imagesetter to prepare corresponding masks on the film. The mask includes a photosensitive area and a non-photosensitive area, such as figure 2 The schematic diagram of the first mask shown, wherein the photosensitive area 100 of the first mask includes a first closed pattern 101 and a strip pattern 102 spaced from the closed pattern 101 at a certain distance, and the first mask also includes a ring-shaped closed pattern 103 , the ring-shaped closed figure 103 is connected with the belt-shaped figure 102, wherein the non-photosensitive unit 300 for forming micropillars is included in the closed figure 103; as image 3 The schematic diagram of the second mask shown, on the basis of the first mask, the closed figure 10...

Embodiment 2

[0064] figure 1 It shows the preparation method of the microfluidic chip according to one embodiment of the present invention. First, enter the mask preparation operation S1100: use CorelDraw software to design two kinds of masks, and use a high-resolution laser imagesetter to prepare corresponding masks on the film. The mask includes a photosensitive area and a non-photosensitive area, such as figure 2 The schematic diagram of the first mask shown, wherein the photosensitive area 100 of the first mask includes a first closed pattern 101 and a strip pattern 102 spaced from the closed pattern 101 at a certain distance, and the first mask also includes a ring-shaped closed pattern 103 , the ring-shaped closed figure 103 is connected with the belt-shaped figure 102, wherein the non-photosensitive unit 300 for forming micropillars is included in the closed figure 103; as image 3 The schematic diagram of the second mask shown, on the basis of the first mask, the closed figure 10...

Embodiment 3

[0067] figure 1 It shows the preparation method of the microfluidic chip according to one embodiment of the present invention. First, enter the mask preparation operation S1100: use CorelDraw software to design two kinds of masks, and use a high-resolution laser imagesetter to prepare corresponding masks on the film. The mask includes a photosensitive area and a non-photosensitive area, such as figure 2 The schematic diagram of the first mask shown, wherein the photosensitive area 100 of the first mask includes a first closed pattern 101 and a strip pattern 102 spaced from the closed pattern 101 at a certain distance, and the first mask also includes a ring-shaped closed pattern 103 , the ring-shaped closed figure 103 is connected with the belt-shaped figure 102, wherein the non-photosensitive unit 300 for forming micropillars is included in the closed figure 103; as image 3 The schematic diagram of the second mask shown, on the basis of the first mask, the closed figure 10...

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Abstract

The invention discloses a method for preparing a micro-flow chip. The method comprises the following step of preparing and operating a masks, namely, designing two masks, wherein the masks comprise light-sensitive areas and non-light-sensitive areas; the light-sensitive area of a first mask comprises a first sealing graph and a banding graph which is isolated from the first sealing graph for a certain distance; the light-sensitive area of a second mask comprises a second sealing graph and a banding graph; the second sealing graph is formed in a mode that the first sealing graph is expanded in parallel till being connected with the banding graph; and the method further comprises a step of carrying out first etching operation, second etching operation and bonding operation. By utilizing the chips prepared by using the method disclosed by the invention, tumor cells can be effectively separated, and moreover the tumor cells after being captured can be directly analyzed.

Description

technical field [0001] The invention relates to a method for preparing a microfluidic chip, in particular to a method for preparing a microfluidic chip for capturing tumor cells. Background technique [0002] Circulating tumor cells usually refer to the cells that come from the primary tumor tissue and enter the peripheral blood of the human body after shedding and infecting. Therefore, circulating tumor cells can be used as an important marker for early tumor diagnosis. In addition, circulating tumor cells also play an important role in tumor metastasis and recurrence. Therefore, it is of great significance to study the physiological mechanism of circulating tumor cells. However, the number of circulating tumor cells and their absolute concentration in human peripheral blood are very low, making the research on circulating tumor cells very difficult, so the sorting and capture of circulating tumor cells is an important research topic. [0003] At present, the commonly us...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N33/574
Inventor 刘威刘侃张南刚国世上周鹏飞
Owner WUHAN YZY MEDICAL SCI & TECH
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