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Kit for extracting and enriching free DNAs and extraction method for free DNAs

A kit and reagent technology, applied in the field of DNA detection, can solve problems such as the toxicity of organic solvents, achieve the effect of simple operation, short time consumption, and meet the clinical use conditions

Inactive Publication Date: 2019-06-21
SHENZHEN APOSTLE SUSTECH LTD
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

[0005] The main purpose of the present invention is to propose a free DNA extraction and enrichment kit and a method for extracting free DNA, aiming to solve the technical problem that organic solvents have certain toxicity in the existing free nucleic acid extraction method

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  • Kit for extracting and enriching free DNAs and extraction method for free DNAs
  • Kit for extracting and enriching free DNAs and extraction method for free DNAs

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Embodiment Construction

[0046] Embodiments of the present invention will be described in detail below, examples of which are shown in the drawings, wherein like reference numerals designate like elements or elements having the same function throughout. The embodiments described below by referring to the accompanying drawings are exemplary and are intended to explain the present invention, but cannot be construed as limitations to the present invention. Based on the embodiments in the present invention, those skilled in the art do not make creative work premise All other embodiments obtained below all belong to the protection scope of the present invention.

[0047] In order to solve the above technical problems, the present invention proposes a free DNA extraction and enrichment kit. The free DNA extraction and enrichment kit includes a sample lysate, a binding solution, a primary washing solution, a secondary washing solution, an eluent and nano magnetic beads. Wherein, the sample lysate compositio...

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Abstract

The invention discloses a kit for extracting and enriching free DNAs. The kit comprises a sample lysate, a binding solution, a primary washing fluid, a secondary washing fluid, an eluent and nano magnetic beads. The sample lysate comprises nuclease-free water, a reagent A and a reagent B, the reagent A is at least one of guanidine isothiocyanate, guanidine hydrochloride, sodium iodide and sodium perchlorate, and the reagent B is at least one of Tween20, Triton-X100, SDS, NP40 and a polyoxyethylene type nonionic surfactant. The binding solution comprises a reagent C in addition to the components of the sample lysate, and the reagent C is at least one of isopropanol and absolute ethanol. The primary washing fluid comprises the same components as the binding solution. The secondary washing fluid is the nuclease-free water, a sodium chloride solution with the molar concentration of 0.1-1M, a Tris buffer solution or a TE buffer solution. The eluent is the nuclease-free water, the Tris buffer solution or the TE buffer solution. The invention further discloses an extraction method for the free DNAs. The technical problem that organic solvents in the prior art have a certain toxicity is solved.

Description

technical field [0001] The invention relates to the field of DNA detection, in particular to a kit for extracting and enriching free DNA and a method for extracting free DNA. Background technique [0002] Nucleic acid includes deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), which are biological macromolecules with genetic information. Blood free DNA (cell free DNA, cfDNA) is referred to as circulating deoxyribonucleic acid, which refers to the partly degraded body's endogenous DNA that is free from the extracellular space in circulating blood; studies have found that free DNA also contains very important genetic information. In recent years, cell-free DNA research has become a hot field in genome molecular diagnosis research. It has been gradually applied clinically to all stages from the initial diagnosis to treatment and development of tumors, including: early screening, evaluation of tumor heterogeneity, metastasis Evaluation of recurrence risk and prognosis, rea...

Claims

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Application Information

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IPC IPC(8): C12N15/10
Inventor 万昊张博葛东亮
Owner SHENZHEN APOSTLE SUSTECH LTD
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