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Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof

A detection method and technology for pathogenic bacteria, applied in biological testing, instruments, measuring devices, etc., can solve the problems of high detection limit, high cost, and cumbersome process.

Inactive Publication Date: 2013-08-14
BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to provide a method for fast and efficient detection of pathogenic bacteria, which can solve the problems of high detection limit, long detection time, cumbersome process and high cost in the prior art

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  • Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof
  • Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof
  • Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof

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[0059] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0060] One of the core concepts of the present invention is to use the unique enzyme activity mechanism of FOF1-ATP (Adenosine-Triphosphate, adenosine triphosphate) enzyme to develop a highly sensitive biosensor FOF1-ATPase pathogenic bacteria detection technology. F0F1-ATPase is a rotary molecular motor that can utilize transmembrane H + Gradient synthesis of ATP, hydrolysis of ATP and reverse transport of H + . During the process of ATP synthesis, protons can be pumped from the inside of the chromoplast membrane to the outside of the membrane, resulting in the inner membrane microenvironment solution H +concentration changes. Connecting loads on F0F1-ATPase will affect its rotation performance in different degrees, and connec...

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Abstract

The invention provides a method for rapidly detecting pathogenic bacteria at high efficiency, a biological dependent sensor and a preparation method thereof, wherein the biological dependent sensor comprises a plastid with the function of converting signals and a capturing system with the function of recognizing molecules; the method for rapidly detecting the pathogenic bacteria at high efficiency comprises the following steps: preparing the biological dependent sensor; using the biological dependent sensor to capture a sample detecting solution; simultaneously setting the blank comparison and the positive concentration comparison of a standard substance and starting anadenosine triphosphate synthetic reaction; and carrying out the detection by utilizing a fluorescence scanner. The schemein the invention has the advantages of low detection limit and short detection time; the detection limit can reach 100cfu / hole; if the detection limit is computed according to 1cfu / 25g, the whole detecting course is not beyond 12 hours, is shortened by at least 60 hours than the conventional microorganism test method and is shortened by at least 36 hours than a PCR (Polymerase chain Reaction) method; and when the concentration of the bacteria in a sample is higher than 1cfu / 25g, the needed time is short, thus the deep influence on food safety and disease treatment bacterium control can be generated.

Description

technical field [0001] The invention relates to the technical field of food safety detection, in particular to a method for rapidly and efficiently detecting pathogenic bacteria, a biosensor and a preparation method thereof. Background technique [0002] In recent years, vicious food safety incidents have occurred frequently around the world. Regardless of developed countries or developing countries, the incidence of food-borne pathogens remains high. The rapid detection of food-borne pathogens has always been the focus of research at home and abroad. . In the prior art, the following detection methods are generally used for rapid detection of foodborne pathogenic bacteria: [0003] Option 1. The traditional microbial method, that is, the detection method specified in GB13091-2002, generally requires the following five consecutive stages: pre-enrichment with non-selective liquid medium for 16 to 20 hours; enrichment with selective liquid medium 24 hours; Streak identificat...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/535G01N33/52
Inventor 武会娟刘清珺魏玲伦永志亢子佳
Owner BEIJING CENT FOR PHYSICAL & CHEM ANALYSIS
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