Method for rapidly detecting pathogenic bacteria at high efficiency, biological dependent sensor and preparation method thereof
A detection method and technology for pathogenic bacteria, applied in biological testing, instruments, measuring devices, etc., can solve the problems of high detection limit, high cost, and cumbersome process.
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[0059] In order to make the above objects, features and advantages of the present invention more comprehensible, the present invention will be further described in detail below in conjunction with the accompanying drawings and specific embodiments.
[0060] One of the core concepts of the present invention is to use the unique enzyme activity mechanism of FOF1-ATP (Adenosine-Triphosphate, adenosine triphosphate) enzyme to develop a highly sensitive biosensor FOF1-ATPase pathogenic bacteria detection technology. F0F1-ATPase is a rotary molecular motor that can utilize transmembrane H + Gradient synthesis of ATP, hydrolysis of ATP and reverse transport of H + . During the process of ATP synthesis, protons can be pumped from the inside of the chromoplast membrane to the outside of the membrane, resulting in the inner membrane microenvironment solution H +concentration changes. Connecting loads on F0F1-ATPase will affect its rotation performance in different degrees, and connec...
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