78results about How to "Fast magnetic response" patented technology

Bio-functionalized magnetic particles have a non-magnetic material matrix which supports core / shell magnetic elements composed from a ferromagnetic core material and a shell material. The shell material can be chosen either among an antiferromagnetic material, a ferromagnetic material of a kind different from the core ferromagnetic material or a metal material. By a proper choice of materials and dimension tuning of both the core and the shell as well as the amount and the concentration of the magnetic elements within the non-magnetic matrix, the bio-functionalized magnetic particles is tailored to exhibit an enhanced magnetic energy. When subjected to an alternating magnetic field, the magnetic particles exhibit specific rotational dynamics in correspondence with the amplitude and the frequency of the applied magnetic filed. Aggregation structures of the magnetic particles are controlled and manipulated by the alternating magnetic field. The magnetic particles are functionalized by specific ligands for the probing and manipulating of biomolecules and chemical substances.

The invention belongs to the technical field of functional material and particularly relates to a magnetic mesoporous titanium dioxide core-shell type compound microsphere as well as a preparation method and applications thereof. The core-shell type compound microsphere takes a ferroferric oxide nano particle cluster as the core and takes mesoporous titanium dioxide with high crystallinity as the shell. The preparation method comprises the following steps: firstly, preparing the ferroferric oxide nano particle cluster with stable citric acid, secondly, wrapping the surface of the cluster with an amorphous titanium dioxide shell through a sol-gel method, and finally, obtaining the magnetic mesoporous titanium dioxide core-shell type compound microsphere through hydro-thermal treatment. By being provided with the mesoporous titanium dioxide shell with high crystallinity, the compound microsphere has the characteristics of high selectivity, high enrichment capacity, high sensitivity and high recovery rate to phosphoeptide; and since a magnetic core exists, the microsphere enriched in phosphoeptide can be separated by magnetism quickly and very conveniently. The method provided by the invention has the advantages that the operation is simple, the process is controllable, and the prepared compound microsphere can be used for analyzing and identifying phosphoeptide with ultra low concentration in a biological sample.

The invention relates to a magnetic Prussian blue carbon nano composite material and a manufacturing method and application thereof, and aims to solve the problems that the conventional magnetic composite material is nonuniform in shape, low in the content of Prussian blue layers, and susceptible to falling off. The manufacturing method comprises the following steps: firstly, magnetic inorganic nano particles are anchored on the surface of a carbon nano material (oxidized graphene / carbon nano tube) with high specific surface area; then, Prussian blue nano crystal layers are grown on the surfaces of the magnetic inorganic nano particles in situ; finally, the composite material is encapsulated into calcium alginate microspheres. The prepared composite material is used for the restoration of caesium ion pollution environments such as water bodies, soil and atmosphere, and has a broad application prospect in the field of nuclear pollution environment restoration. The manufacturing method is simple, uses available raw material, and is suitable for large-scale production.

The invention belongs to the technical field of advanced nano materials, and particularly relates to a magnetic mesoporous silica composite microsphere with a Yolk-Shell structure and a manufacturing method of the magnetic mesoporous silica composite microsphere. Firstly, a sol-gel chemical synthesis method is adopted, and a magnetic nanoparticle is coated with a macromolecule layer of amorphous silicon dioxide and a macromolecule layer of polymer resin in sequence; then the self-assembling behavior of organic surface active agents as structural guiding agents and inorganic substances in solutions is utilized, and the magnetic nanoparticle is coated with a layer of silicon dioxide / surface active agent composite materials with an ordered mesostructure; the surface active agents and the macromolecule layers are removed through roasting, and then the materials of the magnetic mesoporous silica composite microsphere with the Yolk-Shell structure are obtained. The composite microsphere is provided with a large adjustable cavity, a high specific surface area and mesoporous shell layers evenly perpendicular to an interface, has magnetic responsiveness strong in stability, and has wide application prospects in nanoreactors, drug sustained release, high-capacity bioseparation and absorption. The method is simple, raw materials are easy to obtain, and amplification production can be achieved.

The invention belongs to the technical field of advanced nanocomposites, and particularly discloses a magnetic mesoporous silica microsphere material with a yolk structure and a preparing method thereof. The macromolecule sol-gel chemical synthesis method is adopted first, magnetic nanoparticles are coated with a polymer resin macromolecule shell, then a mesoporous silica shell is grown on the surface of the polymer resin shell in a non-contact mode by means of the water-oil two-phase method, a surface active agent and an organic solvent are removed through solvent washing and extraction, and finally the magnetic mesoporous silica composite microsphere material provided with ordered mesopore ducts, a large cavity and the yolk structure is obtained. The obtained composite microsphere has the large adjustable cavity, a high specific surface area, the mesoporous shell with a uniform and vertical interface, stable and high magnetic responsiveness, a quite uniform size and high dispersity and has broad application prospects in nanoreactors, catalysis, drug sustained release, large-volume bioseparation and adsorption. The method is simple, raw materials are easy to obtain, and enlarged production is facilitated.

Bio-functionalized magnetic particles have a non-magnetic material matrix which supports core / shell magnetic elements composed from a ferromagnetic core material and a shell material. The shell material can be chosen either among an antiferromagnetic material, a ferromagnetic material of a kind different from the core ferromagnetic material or a metal material. By a proper choice of materials and dimension tuning of both the core and the shell as well as the amount and the concentration of the magnetic elements within the non-magnetic matrix, the bio-functionalized magnetic particles is tailored to exhibit an enhanced magnetic energy. When subjected to an alternating magnetic field, the magnetic particles exhibit specific rotational dynamics in correspondence with the amplitude and the frequency of the applied magnetic filed. Aggregation structures of the magnetic particles are controlled and manipulated by the alternating magnetic field. The magnetic particles are functionalized by specific ligands for the probing and manipulating of biomolecules and chemical substances.

The invention relates to an immobilized enzyme for a magnetic nanometer clay carrier. The immobilized enzyme comprises a carrier with saccharifying enzyme and corresponding immobilized enzyme as immobilizing objects. The immobilized enzyme is prepared by taking a covalence coupling method as an immobilizing method and is characterized in that: the carrier is a magnetic nanometer Fe3O4@ clay compound material; the magnetic nanometer Fe3O4@ clay compound material is composed of magnetic Fe3O4 nanometer grains and a nanometer clay material; and the magnetic Fe3O4 nanometer grains are assembled on the nanometer clay in sequence. A superparamagnetic Fe3O4@ clay compound material is prepared by adopting a solvent heat method according to the invention; the surface of the Fe3O4@ clay compound material is modified by using gamma-aminopropyl triethoxy silane, and then glutaraldehyde is used as a coupling agent for covalently fixing the saccharifying enzyme on the surface of the carrier; two novel carrier regenerating strategies are designed; after the saccharifying enzyme is immobilized by the regenerated carrier, the excellent properties, such as original activity, thermal stability and reusability, of the immobilized enzyme are still maintained; and the immobilized enzyme is also suitable for the regeneration of the immobilized enzyme carrier according to the covalence coupling method.

The invention discloses water soluble biocompatible fluorescent magnetic nanoclusters and a preparation method thereof. A metal precursor and biocompatible macromolecules are dissolved in a high-boiling-point non-polar or weakly polar solvent, while magnetic nanoclusters are formed through a high temperature decomposition method, the surface of the nanoclusters are modified with the biocompatible macromolecules in situ, and the water soluble biocompatible magnetic nanoclusters with different sizes are prepared; carboxyl and other functional groups carried on the surface of the nanoclusters are coupled with fluorescent molecules, and thus the fluorescent magnetic bifunctional nanoclusters are obtained. The preparation method has the advantages of simple process, easy operation, and low cost of raw materials; the obtained water soluble biocompatible magnetic nanoclusters have the advantages of adjustable size, strong magnetic response and good biocompatibility, can be used as materials of portable biosensors or related environment biosensors, and also can be used in magnetic resonance imaging, fluorescence imaging, cell screening and other biomedical fields.

The invention relates to an antibody-coupled bionic immune magnetic sphere and a preparation method of the antibody-coupled bionic immune magnetic sphere, belonging to the technical field of nanometer materials. According to the bionic immune magnetic sphere, the water-soluble Fe3O4 nanocluster is taken as the core, a cell membrane coats the core, and an antibody with a modification group is bonded outside the cell membrane through covalent coupling. The preparation method of the antibody-coupled bionic immune magnetic sphere comprises the following steps: preparing the Fe3O4 nanocluster, namely, MNC; preparing cell membrane fragments modified by choline azide, namely, M; reacting MCN and M to obtain a bionic magnetic sphere, and reacting the modification group with the antibody to obtain the antibody with the cyclo-ethynylation modification group; and coupling bionic magnetic sphere solution and the antibody with the cyclo-ethynylation modification group, thus obtaining the bionic immune magnetic sphere provided by the invention. The bionic immune magnetic sphere is good in dispersibility, rapid in magnetic response, and high in identifying efficiency when used for capturing tumor cells, no white blood cells exist in the background, and the captured tumor cells are good in activity. For the preparation method, the raw materials are easily available and have universality in the construction of the bionic immune magnetic sphere.

The invention discloses a preparation method of monodisperse silicon oxide magnetic microspheres used for DNA purification and protein separation, which comprises the steps of: modifying magnetic nano particles by adopting a surface chemical modifying agent to obtain an oleophylic and hydrophobic surface and dispersing into an organic solvent, preparing oil drops rich in the magnetic nano particles by using a template-process high-pressure shearing action, stabilizing the oil drops to be suspended in water by means of static endowed by a surfactant and a steric hindrance effect, gradually contracting through the organic solvent in the slowly evaporated oil drops to form a magnetic nano particle complex, wherein the magnetic nano particle complex is a base core; carrying out silicon oxide coating on the complex by adopting a sol-gel method to form a silicon oxide shell with uniform thickness and controllable size; and carrying out alcohol washing and water washing multiple times to finally obtain the monodisperse silicon oxide magnetic microspheres with controllable particle diameter and a core shell structure. The silicon oxide magnetic microspheres prepared by adopting the preparation method have the characteristics of uniform particle diameter, high magnetic response speed and the like, and are easy to realize industrialized mass production.

The invention discloses a preparation method of an epoxy functional core-shell structure magnetic polymer microsphere. The core of the core-shell structure magnetic polymer microsphere disclosed by the invention is ferroferric oxide nanometer crystal druse; the shell layer of the core-shell structure magnetic polymer microsphere is cross-linked polymer containing epoxy functional group. The preparation method comprises the following three steps of: (1), preparing the ferroferric oxide nanometer crystal druse with a high value of saturated magnetic by adopting a solvothermal method; (2), modifying the surface of the ferroferric oxide nanometer crystal druse into a dual-bond functional group by adopting a sol-gel method; (3), coating the polymer shell layer with the epoxy functional group on the surface of the ferroferric oxide nanometer crystal druse by adopting a distillation-precipitation polymerization method to obtain the epoxy functional core-shell structure magnetic polymer microsphere. The epoxy functional core-shell structure magnetic polymer microsphere is narrowly distributed in grain size, controllable in magnetic content, high in surface epoxy radial reaction activity, easy to modify the functional groups including ammonia group, hydroxyl, group, the mercapto group and the like on the surface, capable of being applied to the industrial waste water treatment field by efficiently adsorbing a plurality of heavy meals or organic pollutants, and good in application prospect.

The invention provides an immunomagnetic bead used for capturing a leukocyte and a preparation method thereof. The immunomagnetic bead at least comprises a CD45 immunomagnetic bead and also can comprise or does not comprise one or two of a CD14 immunomagnetic bead and a CD15 immunomagnetic bead, wherein the CD45 immunomagnetic bead, the CD14 immunomagnetic bead and the CD15 immunomagnetic bead are immunomagnetic beads obtained by coupling a CD45 antibody, a CD14 antibody and a CD15 antibody with a magnetic sphere respectively; and a structural formula of the CD45 immunomagnetic bead, the CD14 immunomagnetic bead and the CD15 immunomagnetic bead is A-NH-N=CH-B, wherein A is the magnetic sphere and B is an antibody, or A is the antibody and B is the magnetic sphere. The immunomagnetic bead provided by the invention can be used for capturing the leukocyte, is good in specificity and sensitivity and is rapid in magnetic response, short in enrichment time and high in capturing efficiency; and the preparation method is simple, so that the immunomagnetic bead has strong practicability.

The invention relates to a simple preparation method for a Fe3O4@C@TiO2 magnetic separation photocatalyst. The method includes steps: 1) taking beta-FeOOH nanorods, and dispersing into water to form dispersion liquid; 2) adopting resorcinol and formaldehyde for synthesizing FeOOH@RF core-shell nanorods, and dispersing into ethyl alcohol to form dispersion liquid; 3) adding ethyl alcohol, CH3CN and ammonium hydroxide, and well stirring and mixing to form liquid A; taking ethyl alcohol and CH3CN again, well mixing, and adding TBOT to form liquid B; mixing for room-temperature reaction for a certain time to obtain the nanorods; 4) performing high-temperature calcinations to obtain the Fe3O4@C@TiO2 magnetic separation photocatalyst. The method has advantages that the magnetic-response recyclable Fe3O4@C@TiO2 visible-light nanometer photocatalyst is prepared according to the simple and mild method; the method is simple in operation and low in equipment requirement, and the prepared Fe3O4@C@TiO2 core-shell nanorods are high in dispersity and uniform in nanoparticle size.

The invention belongs to the technical field of advanced nano-composite materials and particularly provides an inorganic magnetic three-dimensional ordered macro-porous (3DOM) material and a preparation method thereof. The preparation method comprises the following steps: firstly, dispersing magnetic nano-particles and polymer microspheres in a solution, so that the gaps formed by arranging the polymer microspheres in an ordered manner can be filled with the magnetic nano-particles by the evaporation self-assembly of the solution; then, filling the remaining gaps of the polymer microsphere / magnetic nano-particle composite material with inorganic oxide sol by the nano-casting method, so that the ordered composite structure can be fixed; and finally, calcining in the presence of nitrogen to remove the polymer microspheres, so as to obtain the 3DOM material. The magnetic 3DOM material has the advantages of higher magnetic response and higher stability, and the 3DOM material is provided with ordered macro-pores which are communicated on a three-dimensional basis. Therefore, the invention is widely applicable in the aspects of bio-separation, drug delivery, catalytic loading and the like. The preparation method is simple and the materials are easily available. Therefore, the invention is suitable for large-scale production.

The invention discloses a photonic crystal hydrogel film and preparation and application thereof. The photonic crystal hydrogel film is prepared from polyacrylamide gel and Fe3O4@C nanoparticles distributed in the polyacrylamide gel, wherein the Fe3O4@C nanoparticles are nanoparticles prepared by coating the surface of Fe3O4 with a C material, the Fe3O4@C nanoparticles are arranged in a chain shape in certain direction in the polyacrylamide gel, and the concentration of the Fe3O4@C nanoparticles in the photonic crystal hydrogel film is 1 to 50mg / mL. According to the photonic crystal hydrogel film disclosed by the invention, categories, sizes and distribution modes of key doped particles in the photonic crystal hydrogel film and a corresponding preparation method are improved, so that compared with the prior art, the problems of formaldehyde detection is expensive and complex to operate are effectively solved, and a novel path is provided for detecting formaldehyde in solutions.

The invention discloses a method for in-situ hydrothermal preparation of a magnetic metal-organic framework core-shell material, relating to preparation of core-shell materials. The invention provides a method for in-situ hydrothermal preparation of a magnetic metal-organic framework core-shell material with the advantages of being simple and convenient in synthesis and free of an additional metal ion source. The method comprises the steps of sequentially adding FeCl3.6H2O, anhydrous sodium acetate and ethylene glycol to a container, mixing evenly, transferring the mixture to a reaction kettle, carrying out heating reaction and cooling to a room temperature; washing an obtained black product by using ethanol and drying to obtain Fe3O4 microspheres; sequentially adding the obtained Fe3O4 microspheres, trimesic acid and water to the reaction kettle, mixing evenly, and carrying out heating reaction to obtain a brown solid sample; and cooling the sample to the room temperature and drying under a nitrogen condition, wherein the obtained sample is the magnetic metal-organic framework core-shell material, namely a Fe3O4@MIL-101(Fe) core-shell material.

The invention belongs to the technical field of the advanced nanometre composite material, particularly to magnetic inorganic nano-particle / zeolite putamen compound microsphere and the preparation method. The invention first adopts the collosol-gel chemical synthesis, wraps a layer of amorphous silica on the external surface of the magnetic inorganic nano-particle, then uses the layer by layer self-assembly technology to adsorb the polyelectrolyte with positive charge and the zeolite nano-particle with negtive charge on the magnetic microsphere surface with negtive charge, finally wraps a layer of zeolite materials on the exterior of the magnetic microsphere through the air-solid phase crystal transformation technology, thereby obtaining the magnetic zeolite compound microsphere with the putamen structure. The compound microsphere has strong magnetic responsibility, and the surface can adsorb a great deal of polypeptide, thereby having wide application foreground on the aspect of bioseparation and high efficiency proteid enzymolysis. The invention has simple method, easy obtaining of the raw materials, which is suitable for the enlarged production.

The invention provides PD-L1 antibody immunomagnetic beads and a preparation method thereof. Each PD-L1 antibody immunomagnetic bead comprises a magnetic microsphere part and an antibody part; the immunomagnetic beads respectively have a structural formula of A-NH-N=CH-B, wherein A represents a magnetic microsphere and B represents a PD-L1 antibody, or A represents a PD-L1 antibody and B represents a magnetic microsphere. When being used for capturing tumor cells, the immunomagnetic beads are not only good in specificity and sensitivity, but also rapid in magnetic response, short in enrichment time and high in capture efficiency. Furthermore, the immunomagnetic beads are stable in properties, small in particle size, good in magnetic responsiveness and good in dispersity. The preparation method is simple and has very high practicality.

The invention discloses a preparation method of a core-shell type boryl magnetic microsphere capable of greatly enriching glycoprotein. The preparation method comprises the following steps: taking ferric trichloride hexahydrate and acetate as raw materials, adopting a solvothermal method to prepare nano ferroferric oxide magnetic particles; adopting a sol-gel method to surface a layer of SiO2 on the surfaces of the nano ferroferric oxide magnetic particles; modifying the magnetic microsphere coated with SiO2, and enabling the surface of the magnetic microsphere to obtain an active vinyl functional group; preparing the core-shell type boryl magnetic microsphere with amino rich in surface through polymerization reaction; modifying a great number of borate groups by the amino in the surface and carboxybenzene borate through amidation reaction, thereby obtaining the core-shell type boryl magnetic microsphere. The core-shell type boryl magnetic microsphere prepared by the preparation methodis uniform in particle diameter distribution, is regular in structure, is controllable in magnetic content, has the characteristics of high magnetic response and surface modification, can separate and purify glycoprotein under a physiological condition, is good in separating ability, and is a biological magnetic separating material with application prospect.

The invention provides a polydopamine multi-level nano-drug carrier, and a preparation method and an application thereof. The preparation method comprises the following steps: S1, uniformly mixing ethanol, ultrapure water and ammonia water, then adding a dopamine hydrochloride solution, carrying out stirring at a room temperature for 25-35 h, and centrifugally collecting precipitates so as to obtain PDA microspheres with a particle size of about 120-350 nm; S2, dissolving the PDA microspheres prepared in the step S1 into ethanol, then adding ferric acetylacetonate and polyol, raising the temperature of the solution to 65-75 DEG C under a vacuum condition, keeping the temperature for 15-30 min so as to remove the ethanol, introducing nitrogen, carrying out heating to 200-220 DEG C, carryingout stirring for 2 h, continuing heating to 280-290 DEG C, carrying out stirring for 50-70 min, carrying out cooling to a room temperature, adding isopyknic acetone, carrying out separating with a magnet, and carrying out washing with ethanol so as to obtain PDA@IOs microspheres; and S3, dispersing the PDA@IOs microspheres obtained in the step S3 into a Tris-HCl buffer solution with a pH value of8-9, and carrying out stirring for 22-26 h so as to obtain the polydopamine multi-level nano-drug carrier, namely PDA@IOs@PDA nanometer microspheres. The nanometer microspheres have metal affinity, good magnetic response and high photo-thermal conversion efficiency.

The invention belongs to the technical field of functional materials, and specifically relates to magnetic composite microspheres with a Raman-enhanced characteristic as well as a preparation method and application thereof. The magnetic composite microspheres take ferroferric oxide / carbon composite microspheres as cores and silver nanoparticles as shells. The preparation method of the magnetic composite microsphere comprises the following steps: firstly, preparing the magnetic ferroferric oxide / carbon composite microspheres; then, reducing silver nitrate liquor by using a reducer to prepare the ferroferric oxide / carbon / silver nanoparticle composite microsphere with uniform and compact silver nanoparticles on the surfaces through an in-situ chemical reduction method. The magnetic composite microspheres provided by the invention have the characteristics of high magnetic responsiveness, high stability, high Raman-enhanced characteristic and high recovery rate. The preparation method is simple in operation and controllable in process, and the prepared magnetic composite microspheres can be used for Raman analysis and detection of trace persistent organic pollutants in food safety and environment.

The invention provides an E-cadherin, Cadherin-11 and EpCAM multi-antibody immunomagnetic bead and a preparation method thereof. The multi-antibody immunomagnetic bead comprises E-cadherin immunomagnetic beads, Cadherin-11 immunomagnetic beads and EpCAM immunomagnetic beads, wherein the E-cadherin immunomagnetic beads, the Cadherin-11 immunomagnetic beads and the EpCAM immunomagnetic beads are immunomagnetic beads respectively obtained by coupling an E-cadherin antibody, a Cadherin-11 antibody and an EpCAM antibody with magnetic microspheres. The multi-antibody immunomagnetic bead is used for capturing CTC and has the effects that the specificity and the sensibility are good, the magnetic response is rapid, the enrichment time is short, and the capturing efficiency is high; and furthermore, the performance is stable, the particle size is small, and the magnetic responsiveness and the dispersity are good. Furthermore, the preparation method is simple and has very strong practicability.

The invention discloses a method for preparing a magnetic field driven intelligent flexible material. The method comprises the following step: by taking mold molding as a preparation method, N,N-dimethyl acrylamide hydrogel as a substrate material, and Fe3O4 nanoparticles as a magnetic response phase, and nano wood plasma cellulose as a reinforcing phase, carrying out in-situ free radical polymerization, so as to obtain the magnetic field driven intelligent flexible material of different magnetic response speeds. The material prepared by using the method is rapid in magnetic field response speed, high in mechanical strength, long in service life, in addition low in production cost, convenient to process and manufacture and wide in application range.

The invention discloses a method for purifying Fe3O4 nano-particles taking oleic acid as a ligand and synthesizing magnetic nano-stirrers and application, relates to a technology for synthesizing themagnetic nano-stirrers, and belongs to the technical field of inorganic nano-material synthesis. The technology comprises the following steps: synthesizing Fe3O4 nano-particles taking oleic acid as aligand; effectively removing iron oleate by-products on the surfaces of particles; carrying out subsequent hydrophilic treatment on the Fe3O4 nano particles; adjustment of assembly mode, realizing effective assembly of the magnetic nanoparticle chain; especially for small-size nano magnetic particles. Pretreatment before particle assembly and adjustment of an assembly mode are provided, assembly of small-size nano chains is achieved, world records of the minimum-size nano chains are broken through, meanwhile, the chain forming probability of the nano stirrer is greatly increased, and high practicability is achieved.

The invention relates to a photochromic room temperature magnetic semiconductor material and a preparation method thereof. The semiconductor material adopts a Bi2WO6 semiconductor material, the morphology of the semiconductor material adopts a lamellar structure; the lamella thickness is 1 to 5nm. The semiconductor material is formed by regulating a bismuth source and tungstate through a high molecular surfactant in a hydrothermal condition. The semiconductor material has the advantages that the morphology of the semiconductor material adopts a lamellar structure, a lamella adopts an ultrathinnanosheet, and the lamellar structure is beneficial to transferring of illuminated electron between a Bi2O2 lamella and a WO6 lamella, so that spin magnetic moment is produced, and photoinduced magnetism is exhibited. The semiconductor material has photochromic performance at a room temperature, and is high in photochromic rate, and the color can be changed after 5 to 10min of irradiation.

The invention discloses a method for preparing carbon-copper-coated ferroferric oxide composite magnetic oil removal particles. The method comprises the following steps of modifying ferroferric oxide,introducing a carbon source, adding a lubricant, an emulsifying and thickening agent and a stabilizer, and then carrying out heat treatment so as to prepare the Fe3O4@C / Cu composite magnetic oil removal particles. The method has the advantages that the technical problems that a floating oil cleaning technology is tedious in preparation process, incapable of realizing mass production and the likecan be solved; and the particles prepared through the method is high in surface carbonization degree and stable in carbon layer, mass production can be realized, and the good particle preparation method solves the problem of floating oil on the water surface.

The invention discloses a method for synchronously enriching and purifying aflatoxin B1 and capsaicine. The method comprises the following steps: mixing bacteria spheres loaded with reduced graphene oxide and obtained by culturing aspergillus flavus fungal spores which do not produce toxin with a precursor of iron, and preparing a magnetic composite nano material by taking ascorbic acid as a reducing agent through a hydrothermal method; adding the material into a sample extracting solution, and fully mixing the materials to adsorb aflatoxin B1 and capsaicine; adsorbing the magnetic composite nano material by using a magnet, and removing the sample extracting solution; adding the purification liquid, fully and uniformly mixing the liquid with the magnetic composite nano material, adsorbingthe magnetic composite nano material by using a magnet, and removing the purification liquid; and finally, adding the eluent, fully and uniformly mixing the magnetic composite nano material, adsorbingthe magnetic composite nano material by using a magnet, and concentrating the collected eluent to obtain a concentrated solution containing a target object. The method is simple and convenient to operate, short in consumed time, low in cost and capable of synchronously enriching aflatoxin B1 and capsaicine.

The invention relates to a functional magnetic material for selective separation of pepsase, wherein the functional magnetic material is prepared by the following preparation steps: preparing ferroferric oxide particles, namely preparing silicon dioxide-coated ferroferric oxide particles by using tetraethoxysilane as a silicon source; adding functional monomers containing glycosyl and positive charges into an aqueous solution, freely polymerizing with allyl triethoxysilane in the presence of an initiator, introducing nitrogen into the mixed solution to remove oxygen, and carrying out a reaction at normal temperature; after the polymerization is finished, adding ferroferric oxide coated with silicon dioxide, and carrying out a reaction in a water bath; and washing the obtained material withdistilled water, and drying to obtain the material. The functional magnetic material disclosed by the invention is good in biocompatibility and good in dispersity; the material has the advantages ofuniform particle size, strong adsorption performance on pepsase, stable performance, realization of repeated use, repeated use for at least six times, realization of the separation of pepsase after the material is placed in a room temperature dry environment for half a year, great saving of the use production cost, and wide application prospect.

The invention discloses a nanometer immune-magnetic bead, and preparation method and a kit thereof. The method comprises the following steps: 1, injecting an iron source and stabilizer mixed liquid into an alkaline solution in an inert atmosphere to obtain magnetic nano-particles, wherein a volume ratio of the alkaline solution to the mixed liquid is 1:(8-10), and a molar ratio of the iron source to the stabilizer to an alkaline substance in the alkaline solution is 1:(5-10):(10-14); and 2, modifying the surfaces of the magnetic nano-particles surface with an antibody having specific immune activity. The preparation method of the nanometer immune-magnetic bead has a simple preparation process and good repeatability. The kit constructed on the basis of the nanometer immune-magnetic bead has the characteristics of good dispersion stability in a target detection liquid, fast magnetic response, strong specificity, high sensitivity and high target capture rate, and has a certain promotion effect on the improvement of the food safety detection efficiency.