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Method for synchronously enriching and purifying aflatoxin B1 and capsaicine

A technology of aflatoxin and capsaicin, applied in chemical/physical processes, material separation, instruments, etc., can solve the problems of non-reusable use and high unit price, and achieve the effects of strong practicability, low price and wide application prospects

Active Publication Date: 2020-01-21
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the enrichment and purification of aflatoxin B1 and capsaicin mainly use immunoaffinity columns, but the unit price of such purification columns is high and cannot be reused
No cheap solid-phase extraction material with both enrichment and purification functions for aflatoxin B1 and capsaicin has been reported yet

Method used

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  • Method for synchronously enriching and purifying aflatoxin B1 and capsaicine
  • Method for synchronously enriching and purifying aflatoxin B1 and capsaicine
  • Method for synchronously enriching and purifying aflatoxin B1 and capsaicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] The first step is to prepare magnetic composite nanomaterials:

[0033] Take 50 g of liquid Sabouraud medium (Qingdao Hi-Tech Industrial Park Haibo Biotechnology Co., Ltd.) dissolved in 1 L of distilled water, autoclave at 115°C for 20 min, and set aside. Take a certain volume of non-toxin-producing Aspergillus flavus fungal spore liquid and add it to 50 mL of sterilized liquid Sabouraud medium, so that the concentration of fungal spores in the liquid medium is 5 × 10 5 cells / mL, seal the Erlenmeyer flask and culture it on a shaker at 28°C for 5 days to obtain mycelium balls with a diameter of 1-2mm. Filter the mycelium balls with 200-mesh nylon cloth, rinse the balls with a large amount of water, and wash them in absolute ethanol for 7 times. Cloth filter, and finally the washed bacteria balls are dried in an oven at 60°C. Weigh 1 g of the above-prepared bacterial spheres into a 100 mL Erlenmeyer flask, add 80 mL of graphene oxide aqueous solution (4.5 mg / mL), shake ...

Embodiment 2

[0037] Determination of parameter conditions in the simultaneous enrichment and purification method of embodiment two:

[0038] (1) Solvent selection for enrichment system

[0039] Weigh 5mg of capsaicin and dilute with pure methanol to a 500mL volumetric flask to prepare a 10μg / mL standard solution. Take 1mg aflatoxin B 1 Dilute with pure methanol to a 1000mL volumetric flask, and configure to obtain a 1μg / mL standard solution. Take 40 μL of the above capsaicin standard solution and aflatoxin B 1 Put 50 μL of the standard solution in a 10 mL centrifuge tube, blow it to dryness with nitrogen, add 10 mL of 3% methanol aqueous solution to make the extract. According to the above steps, adjust the ratio of methanol water added to 5%, 10%, 20%, 30%, 50% and 70%. Also select different ethanol-water ratio extracts. Add 100 mg of synthesized magnetic composite nanomaterials to each extract, mix thoroughly for 5 min, and after magnetic separation, detect the standard sample conte...

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Abstract

The invention discloses a method for synchronously enriching and purifying aflatoxin B1 and capsaicine. The method comprises the following steps: mixing bacteria spheres loaded with reduced graphene oxide and obtained by culturing aspergillus flavus fungal spores which do not produce toxin with a precursor of iron, and preparing a magnetic composite nano material by taking ascorbic acid as a reducing agent through a hydrothermal method; adding the material into a sample extracting solution, and fully mixing the materials to adsorb aflatoxin B1 and capsaicine; adsorbing the magnetic composite nano material by using a magnet, and removing the sample extracting solution; adding the purification liquid, fully and uniformly mixing the liquid with the magnetic composite nano material, adsorbingthe magnetic composite nano material by using a magnet, and removing the purification liquid; and finally, adding the eluent, fully and uniformly mixing the magnetic composite nano material, adsorbingthe magnetic composite nano material by using a magnet, and concentrating the collected eluent to obtain a concentrated solution containing a target object. The method is simple and convenient to operate, short in consumed time, low in cost and capable of synchronously enriching aflatoxin B1 and capsaicine.

Description

technical field [0001] The invention belongs to the technical field of agricultural product quality and safety detection, and in particular relates to a method for synchronous enrichment and purification of aflatoxin B1 and capsaicin. Background technique [0002] After collection by the government and a lot of research on waste oils and fats from kitchens by scholars from relevant research institutions across the country, it was found that due to the dietary characteristics of Chinese people, capsaicinoids will remain in waste oils, and because of the stability of capsaicinoids, they It is often not easy to be removed and becomes one of the markers of kitchen waste grease. In addition, kitchen waste oil is also highly susceptible to aflatoxin contamination because it is often extracted from gutter-like environments. If capsaicin and aflatoxin can be detected simultaneously, the possibility of it being judged as "waste kitchen fat" will be extremely high. Therefore, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01D15/08G01N30/06G01N30/08G01N30/14
CPCB01D15/08G01N30/06G01N30/08G01N30/14G01N2030/062
Inventor 喻理李培武马飞张兆威王督胡小风姜俊
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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