Application of flavonoid glycosides of blancia in improving intestinal barrier function
A technology of blancillin glycosides and intestinal barrier function, which is applied in the field of drug development, can solve problems such as poor effect, lack of safety assessment, and low immune system, and achieve the effect of improving intestinal barrier dysfunction
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Embodiment 1
[0030] Embodiment 1: Preparation and administration of mouse model
[0031] The mice were randomly divided into 3 groups (n=8), namely the control group, the model group, and the flavonoid glucoside group. The normal group was fed with common feed (14.7% J, 13% fat), and the rest of the groups were fed with HFD (21.8% J, 60% fat, D12492), free to eat. After 8 weeks of dietary intervention, except for the normal group, the other two groups were given 120 mg / kg STZ (dissolved in 10 mM citrate buffer, pH 4.0) after fasting for 12 hours. Mice in the normal group were given injection buffer at the same frequency. Each group continued to maintain the current diet, and after one week blood was collected from the tail vein to measure the blood glucose level. When the fasting blood glucose (FBG) level was higher than 11.1 mmol / L, mice were used as T2DM models.
[0032] Each group continued to maintain the current diet. After 8 weeks, the model group was injected intraperitoneally wi...
Embodiment 2
[0033] Example 2: Application of Buliuxing flavonoid glycosides in improving intestinal barrier function in mice
[0034] After 6 weeks, the mice were taken out, anesthetized by injection of 10% chloral hydrate, blood was collected from the eyeballs, and the ileum tissue and feces of the mice were taken from the mice.
[0035] (1) Changes of endotoxin in mouse serum
[0036] After centrifuging the whole mouse blood at low temperature at 12000rpm, take the supernatant, and use the endotoxin assay kit to quantify the expression of endotoxin in the serum of several groups of mice, such as figure 1 As shown in Table 1, the serum endotoxin content in the ileum of the mice in the model group was significantly increased, but after administration of vaccinia flavone glycosides, the serum endotoxin content could be reduced to a normal level.
[0037] (2) Changes of alkaline phosphatase (IAP) in mouse ileum tissue
[0038] The expression of alkaline phosphatase (IAP) in the ileum tiss...
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