52 results about "Firmicutes" patented technology

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The invention discloses a bacillus altitudinis strain and application thereof. The bacillus altitudinis strain is classified and named as Bacillus altitudinis, the preservation unit is the China general microbiological culture collection center (CGMCC), the preservation date is December 28th in 2018, the preservation number is CGMCCNo.17046. The bacillus altitudinis strain belongs to the genus ofbacillus, the family of bacillaceae, the order of bacillales, the class of bacilli, the phylum of firmicutes in the domain of bacteria. The bacillus altitudinis strain can be applied in biological control over root rot of plukenetia volubilis.

The invention provides a lactobacillus plantarum improving easily-fat body and offspring weight and application thereof, and is preserved in the General Microbiology Center of China Microbiological Culture Collection Management Committee, and the preservation number is CGMCC No.14570. The lactobacillus plantarum has excellent acid and bile salt resistance, bile salt hydrolase activity and intestinal colonization ability. Animal experiments and human food experiments show that the lactobacillus plantarum can control weight gain of pregnant women and offsprings caused by a high-fat and high-sugar diet by bile salt hydrolase activity and regulating intestinal flora, at the same time, the lactobacillus plantarum can regulate the intestinal flora, optimize intestinal flora structure, increase number of beneficial bacteria, and improve ratio of bacteroidetes and firmicutes, thereby improving the easily-fat body. The animal experiments and human food experiments show that the lactobacillus plantarum has clear efficacy and high safety, and can reverse the easily-fat body by fundamentally improving the intestinal flora of humans, has a long-lasting non-rebound effect, and has great potential for market development.

The invention discloses cordyceps militaris intracellular polysaccharide and a preparation method thereof, and applications of the cordyceps militaris intracellular polysaccharide in regulation of intestinal flora. The method comprises the following steps: carrying out fermentation culture on cordyceps militaris strains, and separating to obtain cordyceps militaris mycelia; degreasing the cordyceps militaris mycelia, and performing hot water leaching to obtain a cordyceps militaris leaching liquid; and removing protein by adopting trypsin and a Sevage method, carrying out ethanol precipitation, and carrying out DEAE-Sepharose Fast Flow column chromatography purification to obtain the cordyceps militaris intracellular polysaccharide. The cordyceps militaris intracellular polysaccharide prepared by the invention can regulate the intestinal bacterial flora structure of a high-fat diet mouse, promote the growth of intestinal probiotics of high-fat diet mice, improve the proportion of phylum firmicutes / bacteroides, adjust the structure of fungal flora, promote the synthetic route of intestinal butyrate, induce activation of short-chain fatty acid receptors, promote fat metabolism and inhibit fat accumulation so as to achieve the effects of reducing blood fat and protecting the liver.

The invention discloses a bacillus toyonensis strain as well as a preparation method and application of a microbial agent thereof. The preservation department of the bacillus toyonensis SB3018 is China General Microbiological Culture Collection Center (CGMCC), the preservation date of the bacillus toyonensis SB3018 is November 21, 2014, the preservation number of the bacillus toyonensis SB3018 isCGMCC No. 10033; the bacillus toyonensis belongs to the realm of eubacteria, firmicutes, bacilli, bacillaceae and bacillus. The microbial agent of the bacillus toyonensis SB3018 is prepared by carrying out slant culture, seed culture and fermentation on the bacillus toyonensis SB3018. The preparation method comprises the steps of slant culture, seed culture, fermentation and microbial agent preparation; the microbial agent of the bacillus toyonensis SB3018 is applied to preparation of medicines for preventing and controlling tobacco root knot nematode disease and tobacco black shank. The preparation prepared by taking the bacillus toyonensis as an active component has a remarkable killing effect for the tobacco root knot nematode.

The disclosure generally provides a method of balancing microbiome and / or improving nutrient absorption, treating gut inflammation, and / or balancing the level of Firmicutes and Bacteroidetes in the gut of a subject in need thereof, the method comprising consumption by, or administration to the subject of a composition comprising a nutrient in combination with a probiotic composition comprising (i) an isolated non-pathogenic fungal strain and (ii) an isolated non-pathogenic bacterial strain.

A formulation to increase the ratio of gastrointestinal microbiota in phylum Bacteroidetes to microbiota of Firmicutes phylum preferably includes about 20-60 mg / kg of body weight of fermentable fiber, about 10-30 mg / kg of body weight of beta glucan and about 20-60 mg / kg of body weight of blueberry extracts or any fruit or berry ingredient preparation containing similar phenolics. This formulation is preferably used to control body weight, body composition, and blood glucose regulation, preferably in humans, and is preferably administered orally, preferably twice per day.

The invention relates to a special white shrimp breeding composite bacterium flora which comprises 9% of bdellovibrio BDS01, 4% of bdellovibrio BDS02, 5% of nitrobacterium, 3% of streptococcus thermophilus, 12% of yeast, 1.5% of lactobacillus bulgaricus, 1.5% of leuconostoc, 5% of aerobic denitrification bacteria, 0.5% of pediococcus, 5% of bacillus subtilis BDS-2, 2.5% of rhodobacter D8-35, 1% ofactinomycetes S317, 1% of a bacillus B135, 5% of yeast E7, 5% of acetobacterium balch, 5% of methane producing bacteria, 4% of lactic acid bacteria L2, 3% of aerobic spore-bearing bacilli, 2% of flavobacterium, 5% of bacillus coagulans, 3% of anaerobic lactobacillus, 1% of rhodop seudanonas palustris, 0.5% of pseudoalteromonas, 0.5% of rhodobacter azotoformans, 0.5% of firmicutes, 1% of lipoidase, 0.5% of protease, 1% of cyanobacteria, 3% of aerobic denitrification bacteria, 0.5% of actinomycetes, 0.2% of bacillus natto, 0.5% of viable bacteria of clostridium butyricum, 3% of photosynthetic bacteria and 0.8% of photosynthetic bacteria. Harmful indexes such as CODs (Chemical Oxygen Demands), ammonia nitrogen,nitrite and the like can be effectively controlled.

The present invention pertains to a composition comprising garcinol individually or in combination with forskolin for modifying the gut microbial diversity. More specifically, the invention relates to the use of composition comprising garcinol and forskolin for decreasing the Firmicutes / Bacteroidetes ratio and increasing the count of beneficial microbe, Akkermansia muciniphila.

The invention discloses an auxiliary method for detecting transgenic plant components in foods. The method comprises the following steps: 1, separating NF-kB-luc transgenic mice into two groups, wherein the first group is fed by common fodder while the second group is fed by to-be-detected fodder which is a mixture of to-be-detected foods and common fodder; 2, after 10 weeks, taking manure of the mice and extracting total DNA; 3, taking and breaking the total DNA to obtain a DNA segment of 300-800 bp; 4, taking the DNA segment for high-throughput sequencing of 16 S rRNA; 5, based on the results, obtaining composition of intestinal flora, wherein if the relative abundance of firmicutes in the manure of the second group of mice is obviously higher than that of the first group, and statistical difference appears, the to-be-detected foods contain the transgenic plant components. The method is simple and convenient in operation, low in cost and simple in result judgment, and has very high promotion and application values.

The invention discloses a mixed bean dietary fiber and an enzymic method preparation method and application thereof. The mixed bean dietary fiber is prepared from mixed bean cells without cell walls.Plant cell wall Viscozyme L is used for degrading cell walls of mixed beans, the mixed beans include other beans except for soybeans, such as kidney beans, chick peas, mung beans and red kidney beans.The preparation method saves energy and is environmentally friendly; compared with mixed bean starch, the fermentation speed of mixed bean dietary fiber is slower under the in-vitro fermentation condition, fermentation lasts longer, the amount of butyrate generated within 24 h is similar to that of mixed bean starch, the number of Firmicutes bacteria can be specifically increased, the number of bacteroidetes and fusobacteria bacteria is restrained, and the prebiotic function of preventing and treating various metabolism related diseases can be brought into play.

The present invention relates to monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequences homologous to PBP2-a, including pathogenic species such as the methyciline-resistant Staphylococcus Aureus (MRSA), coagulase-negative Staphilococcus, Staphylococcus sciuri and Enterococcus, and any other bacteria containing the PBP2-a protein or homologous sequences. The invention also relates to the use of the monoclonal antibodies capable of recognising and binding to the PBP2-a protein and to other proteins having sequenceshomologous to PBP2-a in a complementary immunodiagnostic test for detecting resistance to beta-lactam antibiotics.

The invention relates to a method for fast and quantitatively measuring the microbial community composition in the fermentation process of baijiu. The method is characterized in that phylum specific primers are used in a PCR system so that special amplification of microbial communities of a certain specific phylum can be performed in the fermentation process of baijiu, quantitative amplification is performed through a qPCR system, and quantitative recognition is achieved. According to the category of known microorganisms, primers of the bacteroidetes, proteobacteria, firmicutes, synergistetes and actinobacteria are respectively designed, and the microstructure change of microbial communities can be fast inspected in the baijiu fermentation process in the level of the phylum on the basis of quantitative amplification of the phylum specific primers.

The invention discloses a method for extracting undaria pinnatifida sulfated polysaccharide. The method includes utilizing undaria pinnatifida powder as a raw material and then conducting enzymatic hydrolysis, enzyme deactivation, sodium alginate removal, CTAB precipitation, dissolution, concentration and desalting and lyophilization to obtain the undaria pinnatifida sulfated polysaccharide. The extraction rate of the undaria pinnatifida sulfated polysaccharide is 0.45%-0.55%. Compared with the other methods, the method achieves the higher extraction rate, and the purity of the obtained undaria pinnatifida sulfated polysaccharide is better, being 86.42%-88.59%. The undaria pinnatifida sulfated polysaccharide can be used for regulating intestinal microorganisms, especially for improving theproportion of firmicutes and bacteroides in the intestinal tract.

The invention provides application of a mesona chinensis extract in preparation of drugs or health-care products for regulating intestinal flora and relates to the technical field of traditional Chinese medicines. According to the application, experiments prove that the mesona chinensis extract can be used for regulating an intestinal flora structure of a high-fat rat, promoting growth of beneficial bacteria, i.e., akkermansia muciniphila, improving a ratio of firmicutes / bacteroidetes, regulating the content of short-chain fatty acids, improving disorder of the intestinal florae, promoting metabolism of fats and inhibiting accumulation of the fats, thereby playing a role in reducing blood fat and protecting liver.

The invention provides a biogas fermentation promotion agent. The promotion agent is prepared from a following microorganism culture solution according to the volume part of the microorganism culturesolution, 15 to 20 parts of firmicutes, 15 to 20 parts of bacteroides, 3 to 5 parts of spirochaeta, 4 to 6 parts of thermotoga, 3 to 5 parts of deltaproteo bacteria, 3 to 5 parts of synergistetes, 3 to 5 parts of cellulomonas and 35 to 40 parts of euryarchaeota. According to the biogas fermentation promotion agent provided by the invention, selected 13 anaerobic fermentation functional strains arepreserved in strain preservation centers at home and abroad and are easy to obtain, culture and compound; according to the fermentation promotion agent provided by the invention, various microorganisms are matched reasonably and can be adaptive to fermentation raw materials with different types so that various raw materials can be rapidly degraded and converted into biogas; according to the biogas fermentation promotion agent, various microorganisms have the same or similar growth culture conditions and the promotion bacterium agent can normally grow and metabolize under the same environmentcondition and play the largest role.

The present invention concerns a new competence stimulating peptide identified in Firmicutes, in particular Streptococcus, and more preferably S. thermophilus and methods of producing transformation competent Firmicutes, in particular Streptococcus, and more preferably S. thermophilus bacteria.

Provided are means, methods, and compositions effective for obesity treatment using probiotics such as bifidobacteria or lactobacilli. Also, a method of selecting probiotic bacteria having a body fat reducing effect, and a method of selecting responders to probiotic bacteria having an anti-obesity action are provided. Provided is a composition for reducing body fat, or the like, which contains Bifidobacterium and / or lactobacilli as an active ingredient, and is used for a pre-obesity group or an obesity disease including intestinal flora in which the ratio of the phylum Firmicutes to the phylum Bacteroidetes has a high value.

The current invention relates to relates to methods of improving commensals in an animal by feeding the animal with a diet including quinoa grain. The quinoa grain is in an amount effective to increase parameters for commensals such as the percentage of lactobacillus in total microbiota, the percentage of bifidobacteria in total microbiota, the percentage of clostridium in total microbiota, and the firmicutes to bacteroidetes ratio. The current invention also relates to pet food compositions that include effective amount of quinoa grain to increase the commensals parameters. In addition, the methods of making such a food compositions are also disclosed.

The present invention provides a prophylactic or therapeutic composition for a metabolic disorder, a composition for amplifying the amount of short-chain fatty acid in the intestine, or a composition for decreasing a ratio of bacteria belonging to the Firmicutes to bacteria belonging to the Bacteroidetes in the intestine, each containing an exopolysaccharide produced by Leuconostoc mesenteroides, and further, a food, a pharmaceutical product, a feed and the like containing the composition.

The invention relates to the field of biological information technology and medical health, and discloses a concept, thought and method for mining a community functional group based on the network analysis technology, and an FBA functional group found from a healthy human oral flora. The functional group is mainly composed of bacteria of the phylum F, the phylum B and the phylum A of actinobacteria, the whole functional group is of a structure that the phylum F and the phylum B synergistically inhibit the phylum A, and the functional group is generally a module with the most species number and the highest density in a healthy oral cavity network. Specifically, the functional group is composed of two sub-functional groups (Sub-Guld), wherein one of the sub-functional groups is mainly composed of F and B mycophylum bacteria, and the other one of the sub-functional groups is mainly A mycophylum bacteria. Species in the two sub-functional groups are in a synergistic relationship, but the two sub-functional groups are in a complete antagonistic competition relationship. The FBA functional group can effectively reflect the stability of the environment in the oral cavity, and a biological foundation is laid for oral health and oral disease healing condition monitoring.

The invention discloses a new application of sodium copper chlorophyllin, relates to the field of medicine, and relates to the application of sodium copper chlorophyllin in the preparation of medicines for treating and regulating intestinal flora. The beneficial effects of the present invention are: the sodium copper chlorophyllin is similar to prebiotics, which balances the microbial flora ecology of the intestinal tract, thereby limiting the growth of conditional pathogenic bacteria and the release of bacterial toxins in the intestinal tract, thereby alleviating liver disease. The present invention verifies through experiments that the sodium copper chlorophyllin can be used to regulate intestinal flora, increase the number of Firmicutes bacteria, and inhibit the abundance of α-proteobacteria and γ-proteobacteria. The imbalance of intestinal flora is closely related to various liver diseases, including liver failure and cirrhosis. Therefore, the preparation of sodium copper chlorophyllin can be used to prevent and treat various liver diseases.

The invention belongs to the technical field of sewer greasy dirt treatment, and particularly relates to a compound bacterium preparation for removing sewer greasy dirt and a preparation method of the compound bacterium preparation. The compound bacterium preparation is prepared from the following raw materials in parts by weight: 30 to 40 parts of proteobacteria fermentation liquor, 20 to 30 parts of thick-walled mycophylum fermentation liquor, 5 to 12 parts of acidobacter fermentation liquor, 15 to 20 parts of bacteroides fermentation liquor, 10 to 16 parts of menosphaeria virens fermentation liquor, 15 to 25 parts of bacillus mycophylum fermentation liquor, 5 to 10 parts of deinococcus-thermophilic mycophylum fermentation liquor and 12 to 20 parts of cyanobacteria fermentation liquor. The concentration of the phylum patellae viable bacteria in the phylum patellae viable bacteria fermentation liquor is greater than 1 * 10 < 8 > / ml. The compound bacteria preparation can effectively digest and absorb oil stains generated in kitchens so as to remove the oil stains in sewers.

Treatment with catestatin changes the proportion of two major phyla Bacteroidetes and Firmicutes in the gut microbiota in an opposite manner observed in intestinal disorders like IBD, IBS or non-intestinal disorders like obesity. Specifically, administration of an effective amount of catestatin increased the relative percentage of Bacteroidetes and decreased the relative percentage of Firmicutes in the gut microbiota.

The invention discloses application of maltopentaglycosyl trehalose and microspheres thereof in regulating intestinal flora, and belongs to the technical field of biology. The preparation method of the maltopentasosyl trehalose microspheres, disclosed by the invention, comprises the following steps: (1) preparing a maltopentasosyl trehalose-sodium alginate mixed solution; and (2) preparing the maltopentaglycosyl trehalose microspheres. The maltopentaglycosyl trehalose microspheres are almost not digested when passing through the upper digestive tracts such as the oral cavity, the stomach, the small intestine and the like, and the maltopentaglycosyl trehalose is protected to completely reach the colon part. According to the microspheres disclosed by the invention, the abundance of pathogenic bacteria is reduced by remarkably improving the abundance of probiotics in an in-vitro fermentation process, so that the intestinal flora of a human body is regulated. Meanwhile, the ratio of the firmicobacteria to the bacteroides can be promoted to be reduced, the host capacity absorption is reduced, and the composition is beneficial to obese people.

The invention discloses a nucleic acid detection kit and detection method for firmicutes and bacteroides. According to the nucleic acid detection kit and detection method of the invention, highly conserved regions of the firmicutes and bacteroides are taken as target regions, and the detection method capable of simultaneously detecting the firmicutes and bacteroides is established by designing and optimizing specific primers and probes. Compared with a traditional bacterial identification method, the method has the advantages of being high in specificity, high in sensitivity, convenient to operate, rapid and accurate in detection and the like; the method can be used for relative quantitative detection of the firmicutes and bacteroides of actual sample intestinal flora; the method can also be used for quantitative detection of the ratio of the firmicutes to the bacteroides; the method is helpful for research on obesity inflammation and type 2 diabetes mellitus, and has important significance for regulating intestinal flora and promoting intestinal health through research on the intestinal flora.