The invention provides a seedlessness roxburgh rose and angelica keiskei compound fruit and vegetable juice drink which is prepared from the following components in parts by weight: 20-80 parts of seedlessness roxburgh rose, 10-80 parts of angelica keiskei, 0-10 parts of stevia rebaudiana, 1-5 parts of citric acid, 0.5-3 parts of newtol, 0.1-0.2 parts of sodium carboxymethylcellulose, 0.01-0.03 parts of xanthan gum, 0.01-0.03 parts of sodium copper chlorophyllin and defined quantity of water. The invention further comprises a preparation method of the seedlessness roxburgh rose and angelica keiskei compound drink, and the preparation method comprises processes of clear juice preparation, mixing, homogenizing, degassing, sterilizing and the like. Prepared seedlessness roxburgh rose and angelica keiskei compound fruit is rich in bioactive constituents such as chalcone, superoxide dismutase (SOD), natural organic germanium, choline, a plurality of families of vitamins, in particualr B12, pantothenic acid, vitamin C and the like and has abundant nutrition and health care effects. Simultaneously, products are good in taste and tasty in sour and sweet degree.