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Overall extraction method of effective components of mulberry leaves

The technology of an active ingredient and an extraction method, which is applied in the field of overall extraction of active ingredients from mulberry leaves, can solve the problems of unsuitability for industrial production, low content of extracts from various ingredients, toxicity and the like.

Active Publication Date: 2016-01-27
上海天伟质量检测技术服务有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that only the effective components of mulberry leaves extracted by hydrophilic solvents are used, and the utilization of alcohol-soluble substances and insoluble substances in mulberry leaves is not considered, resulting in serious waste of raw materials.
The disadvantage of this method is that only a very small part of the ingredients in the mulberry leaf alkaloids (accounting for 0.1%-0.3% of the mulberry leaf content) is used, resulting in a serious waste of raw materials
[0006] Chinese patent: CN200810236144.1 discloses a compound extraction process for the co-production of mulberry leaf flavonoids, polysaccharides and alkaloids. The main steps are: raw material treatment, water extraction, macroporous resin adsorption and separation, in which chloroform is used as the extraction agent, which is toxic , it is difficult to remove in the production process, not suitable for industrial production
The disadvantage of this method is that the content of the obtained multi-component extract is too low, and there is little commercial benefit without separation
The disadvantage of this method is that the active ingredients obtained are not high when extracted with acidified deionized water, the final product types are few, the mulberry leaf resources cannot be fully utilized, and the purity of the single product obtained is low
The disadvantage of this method is that using microfiltration and ultrafiltration technology to obtain the extract is slow and costly, which is not conducive to industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] (1) Wash and dry the mulberry leaves, crush them through a 40-mesh sieve, add them into the 90v / v% ethanol-water solution at a concentration of 2:15 (W / V), stir at a high speed for 8 minutes, and mix well at a temperature of 30°C. Under the conditions of power 6KW and frequency 20KHz, high-speed stirring and ultrasonication for 1.5h, and centrifugation to obtain a primary alcohol extraction filtrate and filter residue.

[0088] (2) Add the filter residue into the ethanol-water solution with a concentration of 60v / v% at a ratio of 1:15 (W / V), repeat the extraction for 0.8h at a temperature of 50°C under the same ultrasonic conditions, and centrifuge to obtain the second alcohol extraction filtrate and filter residue.

[0089] (3) Combine the two alcohol extraction filtrates to obtain the alcohol extract of mulberry leaves.

[0090] (4) Put the alcohol-extracted filter residue into the drum, and spin to remove ethanol at a temperature of 50°C and a vacuum of 0.05 MPa unt...

Embodiment 2

[0114] (1) Wash and dry the mulberry leaves, crush them through a 40-mesh sieve, add them into the ethanol-water solution with a concentration of 90v / v% at a ratio of 1:8 (W / V), stir at high speed for 8min and mix evenly, at a temperature of 30°C, ultrasonically Under the conditions of power 6KW and frequency 15KHz, high-speed stirring and ultrasonication for 1h, and centrifugation to obtain the primary alcohol extraction filtrate and filter residue.

[0115] (2) Add the filter residue into the ethanol-water solution with a concentration of 75v / v% at a ratio of 1:10 (W / V), repeat the extraction for 1 hour at a temperature of 45°C under the same ultrasonic conditions, and centrifuge to obtain the secondary alcohol-extracted filtrate and filter residue .

[0116] (3) Combine the two alcohol extraction filtrates to obtain the alcohol extract of mulberry leaves.

[0117] (4) Put the alcohol-extracted filter residue into the rotating drum, and spin to remove ethanol at a temperatu...

Embodiment 3

[0140] (1) Wash and dry the mulberry leaves, crush them through a 40-mesh sieve, add them into the 80v / v% ethanol-water solution at a concentration of 1:10 (W / V), stir at a high speed for 8min and mix evenly, at a temperature of 45°C, ultrasonically Under the conditions of power 6KW and frequency 15KHz, high-speed stirring and ultrasonication for 1h, and centrifugation to obtain the primary alcohol extraction filtrate and filter residue.

[0141] (2) Add the filter residue into the 70v / v% ethanol-water solution at a ratio of 1:8 (W / V), repeat the extraction for 1 hour at a temperature of 45°C under the same ultrasonic conditions, and centrifuge to obtain the secondary alcohol-extracted filtrate and filter residue .

[0142] (3) Combine the two alcohol extraction filtrates to obtain the alcohol extract of mulberry leaves.

[0143] (4) Put the alcohol-extracted filter residue into the rotating drum, and spin to remove ethanol at a temperature of 50°C and a vacuum of 0.05 MPa un...

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PUM

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Abstract

The invention relates to an overall extraction method of effective components of mulberry leaves. The method comprises the steps that mulberry leaves serve as raw materials, ultrasonic alcohol extraction and water extraction are sequentially conducted to obtain alcohol extract, water extract and extracted filter residues, concentration, extraction and decoloration are conducted on the alcohol extract, enrichment and elution are conducted through anion exchange resin and cation exchange resin to obtain mulberry leaf flavone and DNJ, and saponification, copper substitution reaction and salifying are conducted on removed pigment to prepare chlorophyll sodium copper; alcohol precipitation is conducted after concentration is conducted on water extract, protein and polysaccharide are clarified and separated through a clarifying agent, spray drying is conducted on the polysaccharide to prepare mulberry leaf polysaccharide, the protein is added to conduct enzymatic hydrolysis, and micromolecule oligopeptide is prepared; ultrasonic alkali washing is conducted on extracted residues, soak degreasing is conducted on ethyl alcohol, and mulberry leaf dietary fibers are prepared; by means of the overall process, the mulberry leaves are completely dissolved in a one-station mode, six main products are prepared, the effective components in the mulberry leaves are fully utilized, and the additional value is increased; compared with single substance extract, the huge profit space and economic value exist, good business prospect is achieved, and the overall extraction method of the effective components of the mulberry leaves is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the technical field of mulberry leaf extraction, and relates to an overall extraction method for effective components of mulberry leaves, which is a step-by-step and efficient method for extracting multiple active components in mulberry leaves, that is, extracting mulberry leaves with high-concentration alcohol The target product is disassembled, and then the next step of targeted extraction process is carried out to obtain mulberry leaf polysaccharides, mulberry leaf flavonoids, mulberry leaf chlorophyll, mulberry leaf dietary fiber and mulberry leaf 1-deoxynojirimycin. method. Background technique [0002] Mulberry leaf is the dry leaf of Moraceae plant mulberry (Morusalba L.), which is the main feed of traditional silkworms and the by-product of fruit mulberry. It is produced in most parts of my country and can be collected all year round; its sources are widely available, Abundant resources and cheap prices. It tastes bitt...

Claims

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Application Information

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IPC IPC(8): C07D487/22C07D211/46C07D311/40C08B37/00C12P21/06
CPCC07D211/46C07D311/40C07D487/22C08B37/0003C12P21/06
Inventor 李森原王济强何秋旻谈学马陈莹
Owner 上海天伟质量检测技术服务有限公司
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