36 results about "Copper oxidase" patented technology

The invention relates to a processing device of ammoniacal odor and spray liquid and operation method thereof, belonging to the filed of environmental protection. The processing device mainly comprises a biotrickling filtering tower (4), a washing tower (15), a hybrid regulating reservoir (24) and an anaerobic sequence batch reactor (29). The ammoniacal odor enters the biotrickling filtering tower (4) and the washing tower (15) after diffluence, gas purified through the two towers can reach emission requirement, the concentration of NOx in the recycled spray liquid inside the biotrickling filtering tower is gradually increased, and the concentration of NH4 in the recycled spray liquid inside the washing tower is gradually increased. The drainage frequency and amount of spray liquid in the two towers are controlled so that the spray liquid is merged into the hybrid regulating reservoir (24) and enters the anaerobic sequence batch reactor (29) after water quality is adjusted, and the effluent after anaerobic amine oxidase denitrogenate processing is used as spray water of biotrickling filtering tower and washing tower and thinned water of hybrid regulating reservoir. The invention has high odor clearance; on the premise that the system has good operation, spray water is cycled in the inner loop without secondary pollution, and the water source is saved.

The invention discloses escherichia coli recombinant bacteria capable of high-producing 2, 5-dimethylpyrazine and a construction method of the escherichia coli recombinant bacteria, and belongs to thetechnical field of gene engineering. According to the escherichia coli recombinant bacteria and the construction method thereof, a genetic engineering method is applied, L-threonine dehydrogenase TDHis overexpressed in escherichia coli K-12 capable of high-producing L-threonine, and NADH oxidase NoxE derived from lactococcus microorganisms and aminoacetone oxidase AAOSO derived from streptococcus microorganisms are expressed in a heterologous manner, and meanwhile 2-amino-3-ketobutyric acid CoA ligase KBL and primary amine oxidase TynA are knocked out, so that a novel and efficient 2, 5-dimethylpyrazine synthetic route is constructed, and the problem of unbalance of cofactors in the recombinant bacteria is solved. By taking escherichia coli E. coli THR as an example, the accumulation amount of 2, 5-dimethylpyrazine reaches 1.2 +/-0.2 g/L through a 36h shaking flask fermentation experiment of the recombinant bacteria. According to the method, L-threonine high-producing strains are used as starting strains, the synthetic route of 2, 5-dimethylpyrazine in escherichia coli is successfully reconstructed, the defect of unbalanced intracellular cofactors is improved, and a new idea is provided for breeding 2, 5-dimethylpyrazine.

The present invention relates to a method for the deracemisation or chiral inversion of chiral amines by enzymatic treatment. The method employs a stereoselective enzymatic conversion and either a non-selective or partially selective chemical or enzymatic conversion, simultaneously or sequentially. The invention also provides a method for selecting a suitable enzyme, particularly a suitable amine oxidase, and for the generation of novel enzymes suitable for use in the deracemisation method.

The invention discloses preparation of lactic acid bacteria multi-copper oxidase and application of the lactic acid bacteria multi-copper oxidase in biogenic amine degradation, and belongs to the technical field of bioengineering. The multi-copper oxidase provided by the invention is derived from Lactobacillus sakei, and is produced by carrying out heterologous expression on the Lactobacillus sakei in escherichia coli. The enzyme has good biogenic amine degradation performance, and can degrade 40.90% of histamine and 41.24% of tyramine at a low concentration. Under the high-salt condition, the amine reduction performance can be further improved, and the histamine degradation rate and the tyramine degradation rate are 75.95% and 66.81% respectively. And partial amine reduction capability can be maintained under a high ethanol condition. The enzyme has a good amine reduction effect when applied to simulation systems of fermented foods such as salted fish and grape wine, and can be widely applied to industrial production.

The invention discloses a corn multi-copper oxidase coding gene ZmDEK559-2, the CDS sequence of the gene is shown as SEQ ID No.1, and the sequence of a coded protein is shown as SEQ ID No.2. The invention also discloses application of the corn multi-copper oxidase coding gene ZmDEK559-2 or a plant expression vector containing the gene in improvement of drought or high-salt adversity stress tolerance breeding of crops. Experiments prove that the drought resistance, salt resistance and other characters of the ZmDEK559-2 gene overexpression corn are obviously improved compared with those of an untransformed wild type control, and the ZmDEK559-2 gene overexpression corn can keep relatively good growth vigor when encountering adversity stress such as drought and high salt, so that the increaseof the crop yield is promoted, and it is indicated that the gene has wide application prospects in crop stress resistance genetic breeding improvement.

The invention discloses a copper-containing amine oxidase sourced from saccharopolyspora cavaleriei and capable of degrading biogenic amine and application thereof, and belongs to the technical field of molecular biology. The copper-containing amine oxidase is screened from saccharopolyspora cavaleriei, has relatively strong biogenic amine degradation performance, is wide in degradation spectrum and has certain tolerance to ethanol, the total biogenic amine degradation rates of commercially available yellow rice wine and commercially available soy sauce are 30.25% and 18.29% respectively, the copper-containing amine oxidase has certain application value in fermented wine such as yellow rice wine and grape wine, and the safety of the fermented food can be further improved.

The present invention relates to novel compounds which are capable of inhibiting certain amine oxidase enzymes. These compounds are useful for treatment of a variety of indications, e.g., fibrosis, cancer and/or angiogenesis in human subjects as well as in pets and livestock. In addition, the present invention relates to pharmaceutical compositions containing these compounds, as well as various uses thereof.

The invention discloses plant source amine oxidase as well as an extraction method and application thereof. The extraction method comprises the following steps: culturing plant tissues and extracting amine oxidase from the plant tissues. The enzyme provided by the invention is a plant-derived enzyme, so that the safety is higher; when common biogenic amine in food is taken as a substrate, obvious enzyme activity can be detected. And moreover, the amine-reducing enzyme has relatively strong degradation capability on several common biogenic amines in food. The method can be used in various foods with relatively high biogenic amine content; the high-histamine fish products, such as high-histamine fishes, shrimp paste, fish sauce, red wine, fermented vegetables, beer, ham, salted fish and spicy fermented tea, and common foods with high amino acid and protein contents, such as cereal fermented products, bean fermented products, milk fermented products, meat fermented products, fruit and vegetable fermented products, aquatic product fermented products and fermented tea, can be added into the high-histamine fish products, the cereal fermented products, the bean fermented products, the milk fermented products, the meat fermented products, the fruit and vegetable fermented products, the aquatic product fermented products and the fermented tea. And biogenic amines therein are degraded.

The invention discloses sea buckthorn fruit extract moisturizing gel and a preparation method thereof. The sea buckthorn fruit extract moisturizing gel comprises the substances of sea buckthorn fruitpropyl gallate, tryptophan, amine oxidase, oleanolic sterol ester, sea buckthorn fruit sitosterol, oleanolic acid, glycerol, bletilla striata gum, normal propyl alcohol, a preservative, essence, deionized water and the like. Gallic acid has anti-inflammatory biological activity, esterification reaction and in-vivo SOD (superoxide dismutase) combined reaction are performed, and the effects of preventing aging, resisting oxidation and preventing allergy are achieved while water is replenished. The amine oxidase can remove free radicals generated during enzymatic reaction and non-enzymatic reaction in the body and remove toxic substances in the body. The oleanolic sterol ester improves the activity of oxidase in vivo, regulates lipid stability and corrects protein metabolic disorders. By adding the moisturizing anti-aging antioxidant substance and the reaction of the moisturizing anti-aging antioxidant substance in the body, the effects of preventing allergy, enhancing moisture retention,improving lipid metabolism and the like are achieved.

The invention relates to an amine oxidase NDAO. The amino acid sequence of the amine oxidase NDAO is SEQ ID NO. 1. The degradation rate of the amine oxidase NDAO provided by the invention on HFB1 is 100%. Amine oxidase NDAO is excellent in property, can be applied to industries such as agriculture, feeds and foods, and reduces harm of Fumonisins FB1 to animal and human health.

The present invention is directed to hydrazino compounds that functions as inhibitors of copper-containing amine oxidases commonly known as semicarbazidesensitive amine oxidases (SSAO), including the human SSAO known as Vascular Adhesion Protein-1 (VAP-1). These SSAO inhibitors have therapeutic utility as drugs to treat conditions and diseases including, but not limited to, a number of inflammatory conditions and diseases (in particular chronic inflammatory conditions such as chronic arthritis, inflammatory bowel diseases, and chronic skin dermatorses), diseases related to carbohydrate metabolism and to aberrations in adipocyte differentiation or function and smooth muscle cell function, and vascular diseases. The compounds have general formula: or a pharmaceutically acceptable solvate, hydrate, or salt thereof, wherein R<1> to R<8> and X are as defined herein.

The invention relates to pyridazinone and pyridone compounds having formula (I) or (I′), and pharmaceutically acceptable salts, hydrates, and solvates thereof (I) wherein R₁/R₄ and X and X₃ are as defined in the claims. The invention further relates to their use as inhibitors of copper-containing amine oxidases. The present invention also relates to the preparation of the aforementioned compounds and to pharmaceutical compositions comprising as an active ingredient(s) one or more of the aforementioned compounds, pharmaceutically acceptable salts, hydrates, or solvates thereof.

The invention relates to amine oxidase ASAO. The amino acid sequence of the amine oxidase ASAO is as shown in SEQ ID NO. 1. The degradation rate of the amine oxidase ASAO provided by the invention on HFB1 is 100%; and amine oxidase ASAO is excellent in property, can be applied to industries such as agriculture, feeds and foods, and reduces harm of Fumonisins FB1 to animal and human health.

The invention provides a method for preparing N-methyl pyrroline through combined catalysis of three enzymes. The method comprises the following steps: taking L-ornithine as a substrate, and carryingout combined catalysis by using ornithine decarboxylase EcODC, putrescine N-methyltransferase AtPMT and amine oxidase AaDAO3 to obtain the N-methyl pyrroline. The method can also be realized by fermentation of a genetically engineered bacterium expressing the three enzymes. The method is environmentally friendly, has mild reaction conditions, and has an industrial development prospect.

The invention belongs to the technical field of fine chemical synthesis, and particularly relates to an indoline isochroman derivative as well as an enzymatic synthesis method and application thereof. 2,3-dihydroxy benzoic acid and an indole compound are taken as substrates, multi-copper oxidase is taken as a catalyst, and the indoline isochroman derivative is obtained through reaction. The indoline isochroman compound with high added value is obtained from cheap and easily available chemical raw materials through the oxidative coupling reaction catalyzed by using the multi-copper oxidase as the catalyst, and the method is mild in reaction condition, simple to operate, low in cost, green and environmentally friendly.

The invention discloses recombinant escherichia coli for efficiently producing glutaric acid as well as a construction method and application of the recombinant escherichia coli. A double-plasmid recombinant strain is constructed by means of molecular biology, an aromatic aldehyde synthase gene AAS, an amine oxidase gene Mao and an aldehyde dehydrogenase gene Glox are co-expressed, and constructed expression plasmids are introduced into escherichia coli to construct recombinant cells. And carrying out ampicillin resistance and kanamycin resistance combined plate screening to obtain a recombinant strain for efficiently producing glutaric acid. And the efficient production of glutaric acid is realized by optimizing the substrate concentration, the thallus concentration and the conversion temperature. The L-lysine with the concentration of 30 g/L can be converted into glutaric acid with the concentration of 19.65 g by reacting for 30 h under the conversion conditions that the thallus concentration is 30 g/L, the pH value is 8 and 6 mM of NAD < + > is additionally added, and the conversion rate can reach 65.3%.