31 results about "Sulfhydryl oxidase" patented technology

The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.

The present invention is directed to the treatment of reactive thiol groups (-SH) found in thiol-containing flavor compounds by a highly selective enzymatic conversion into aroma-active disulides compounds using sulfhydryl oxidase.

Biomarkers for pre-Diabetes, Diabetes and / or a Diabetes related conditions, and methods of their use, including the biomarkers in Tables 1 and 2 such as peroxiredoxin-2, complement C1q subcomponent subunit B, sulfhydryl oxidase 1 and apolipoprotein A-IV.

Disclosed is as a biomarker useful in early diagnosis of lung cancer, at least one protein selected from the group including Quescin-sulfhydryl oxidase 1, Fibrillin-1, Isoform A of Lamin-A / C, Latent-transforming growth factor beta-binding protein 2, Galectin-1, highly similar to Dickkopf-related protein 3, Isoform Al—B of Heterogeneous nuclear ribonucleoprotein Al, 14-3-3 protein epsilon, Stanniocalcin-2, Cystatin-C, Isoform 1 of Connective tissue growth factor, Profilin-1, Isoform 1 of Extracellular matrix protein 1, Histone H2B type 2-E, Kinesin-like protein KIF26A, Zinc finger protein 516, and Isoform 1 of A-kinase anchor protein 9.

The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.

The invention relates to a method for preparing sodium 3,3'-dithiodipropane sulfonate by using a biological enzyme method. The method comprises the following steps: S1, preparing a sodium 3-mercaptopropanesulphonate solution with the concentration of 15 to 30 percent; S2, selecting sulfhydryl oxidase as a catalyst and adding the sulfhydryl oxidase into the solution prepared in the step S1, and enabling the solution to continuously pass through air; meanwhile adjusting a pH value of the solution to 6.5 to 7.5, and carrying out aqueous-phase catalytic oxidation reaction; S3, after the catalytic reaction is finished, filtering the solution by using an ultrafiltration membrane, removing enzyme protein in the solution and carrying out decolorization; S4, carrying out concentrated dehydration on filtrate obtained in the step S3 by a thickener, thus obtaining the sodium 3,3'-dithiodipropane sulfonate. The method disclosed by the invention has the advantages of mild conditions, short process flow, simple steps, no pollution to the environment, low energy consumption and high efficiency.

The present invention relates to a method for producing a protein of interest containing one or more disulfide bonds in its native state. The method comprises that a prokaryotic host cell is genetically engineered to express the protein of interest and a sulfhydryl oxidase in the cytoplasm of the host cell. The protein of interest is formed in a soluble form and contains disulfide bonds due to the presence of the sulfhydryl oxidase in the cytoplasm of said host cell. The present invention relates also to a prokaryotic host cell and a vector system for producing a protein of interest containing natively folded disulfide bonds.

The present invention provides methods for tumor treatment by administering an inhibitor of quiescin sulfhydryl oxidase 1 (QSOX1), compositions comprising such inhibitors, and methods for identifying such inhibitors.

Disclosed is as a biomarker useful in early diagnosis of lung cancer, at least one protein selected from the group including Quescin-sulfhydryl oxidase 1, Fibrillin-1, Isoform A of Lamin-A / C, Latent-transforming growth factor beta-binding protein 2, Galectin-1, highly similar to Dickkopf-related protein 3, Isoform A1-B of Heterogeneous nuclear ribonucleoprotein A1, 14-3-3 protein epsilon, Stanniocalcin-2, Cystatin-C, Isoform 1 of Connective tissue growth factor, Profilin-1, Isoform 1 of Extracellular matrix protein 1, Histone H2B type 2-E, Kinesin-like protein KIF26A, Zinc finger protein 516, and Isoform 1 of A-kinase anchor protein 9.

The invention provides a bioenzyme-containing diesel oil booster composition, comprising a cetane number improver and a bioenzyme at a weight ratio of 1:0.01-1; the bioenzyme is sulfonase, alcohol oxidase, aldehyde Oxidase, amine oxidase, sulfhydryl oxidase, methylmercaptan oxidase, menaquinone oxidase, peroxidase, ferrochelatase, decarboxylase, aldolase, oxoacid lyase, demethylation Mixture of enzymes, alcohol, acid, ester dehydratase, desulfhydrylase, desulfomethylase, cobalt chelatase, magnesium chelatase. A bio-enzyme-containing diesel oil booster composition of the present invention can realize high-speed catalytic performance in a mild environment by utilizing the characteristics of a bio-enzyme, and is used in combination with the existing cetane number improver, The catalytic combustion efficiency of the cetane number improver in diesel is significantly improved, and the combustion performance of diesel is further improved.

The invention belongs to the field of food additives, and particularly discloses a high-efficiency bread flour modifying agent. The high-efficiency bread powder modifying agent is prepared by taking alpha-amylase, thiol oxidase, hemicellulase, lipase, azodicarbonamide, glycerol monostearate, calcium stearoyl lactate, diacetyl tartaric acid monoglyceride and modified starch as raw materials; and uniformly mixing the raw materials in certain weight percentage. The high-efficiency bread flour modifying agent has simple preparation, is green and safe, can effectively improve the processing performance of bread flour, obviously increases the volume of bread, improves internal structures, makes the surface of the bread smooth and elastic, and can prolong quality guarantee period of the bread.

The invention relates to the technical field of natural colored silk processing, in particular to a complex enzyme for degumming and fixation of natural colored silk and an application method of the complex enzyme. The complex enzyme comprises a bio-enzyme A and a bio-enzyme B; the bio-enzyme A comprises, by weight, 1-10% of papain, 1-10% of bromelain, 0.5-5% of neutral protease, and 0.1-3% of lipase; the bio-enzyme B comprises, by weight, 1-10% of sulfhydryl oxidase and 0.3-5% of protein disulfide isomerase. With the complex enzyme and the application thereof, the biggest obstacle in promotion and application of colored cocoons in the later period is omitted, the pure-natural style of colored silk is guaranteed, the dyeing and finishing process is omitted, and the high-quality environmental protection concept is met.

The invention discloses a soluble expression system and application thereof in soluble expression of protein. The inventor screens protein disulfide bond isomerase from different sources, and constructs the protein disulfide bond isomerase and sulfydryl oxidase under the control of the same promoter. The expression of the two proteins is optimized through a strategy of adding a nitrogen terminal tag, and a soluble expression system is constructed. In escherichia coli, after co-expression of the system and target protein recombinant porcine carboxypeptidase B, soluble expression of the porcine carboxypeptidase B can be realized, and formation of inclusion bodies is greatly reduced. The soluble expression system can also be used for soluble expression of other target proteins, such as trypsinogen, and has universality of promoting soluble expression of the target proteins. The method has application prospects in the fields of recombinant protein production, new enzyme original screening and the like.

The invention relates to the enzymatic treatment of a proteinaceous substrate with a first enzyme, such as a sulfhydryl oxidase. The first enzyme removes enzyme inhibitors, such as free thiols, present in the proteinaceous substrate. The removal of the inhibitory compounds in the substrate allows for an effective enzymatic action of a second enzyme such as protein cross-linking of the protein present in the substrate by tyrosinase enzymes.

Methods for isolating a highly purified QSOX enzyme from milk or whey are described. The enzyme may be used to generate intermolecular and intramolecular disulfide bridges and to facilitate oxidative protein folding.