Amine oxidase ASAO, preparation method and application

An amine oxidase, separation and purification technology, applied in the field of agricultural biology, can solve the problems of less research and scarce biodegradation methods, and achieve the effect of reducing harm

Active Publication Date: 2021-12-21
TIANJIN UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the advantages of biodegradation over physical and chemical degradation, there are still relatively few studies related to it, and there are even fewer biodegradation methods that can be effectively used for commercial purposes.

Method used

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  • Amine oxidase ASAO, preparation method and application
  • Amine oxidase ASAO, preparation method and application
  • Amine oxidase ASAO, preparation method and application

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Embodiment Construction

[0025] The embodiments of the present invention will be described in detail below. It should be noted that the embodiments are illustrative, not restrictive, and cannot limit the protection scope of the present invention.

[0026] The raw materials used in the present invention, unless otherwise specified, are conventional commercially available products; the methods used in the present invention, unless otherwise specified, are conventional methods in the art.

[0027] An amine oxidase ASAO, the amino acid sequence of the amine oxidase ASAO is SEQ ID NO.1.

[0028] The preparation method of the above-mentioned amine oxidase ASAO, the steps are as follows:

[0029] (1) Using molecular biology means to recombine the gene encoding the amine oxidase ASAO into a vector and then transform the host cell to obtain a recombinant strain;

[0030] (2) Cultivate the recombinant strain and induce the expression of amine oxidase ASAO;

[0031] (3) Separating and purifying amine oxidase A...

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Abstract

The invention relates to amine oxidase ASAO. The amino acid sequence of the amine oxidase ASAO is as shown in SEQ ID NO. 1. The degradation rate of the amine oxidase ASAO provided by the invention on HFB1 is 100%; and amine oxidase ASAO is excellent in property, can be applied to industries such as agriculture, feeds and foods, and reduces harm of Fumonisins FB1 to animal and human health.

Description

technical field [0001] The invention belongs to the field of agricultural biotechnology, in particular to an amine oxidase ASAO, a preparation method and application. Background technique [0002] Fumonisins (FB) are structurally similar secondary metabolites produced by Fusarium and other fungi under specific conditions. Food crops are a necessity for human survival. However, some mycotoxins are particularly easy to contaminate food crops, and when these food crops are processed into some agricultural products, they cannot be removed well, and will enter the human body along the food chain, causing certain harm to the human body. Fumonisins are mainly found in animal feed materials and their products, which can cause acute poisoning of some livestock and are potentially carcinogenic to most poultry. At the same time, there is the possibility of human infection. Some studies have shown that fumonisins Toxins are closely related to the pathogenesis of human esophageal cance...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/06C12N15/53C12N15/70C12N1/21A23L5/20C12R1/19
CPCC12N9/0022C12N15/70A23L5/25C12Y104/03006
Inventor 李中媛夏忠强张同存
Owner TIANJIN UNIV OF SCI & TECH
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