New competence stimulating peptide
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1) The Anti Transporter Controls the Synthesis of Several Proteins Essential for Natural Transformation in Streptococci
[0083]In order to find physiological functions controlled by signaling peptides that are internalized by the Ami transporter, we compared the proteome of the wild type LMD-9 strain and its isogenic mutant deleted for the ami operon, LMD-9 ΔamiCDE (TIL883; IBRAHIM et al., J. Bacteriol., vol. 189, p: 8844-8854, 2007).
[0084]In order to by-pass the nutritional function of the Ami transporter, cells were gown in CDM, a free-peptide chemically defined medium (CDM), containing only amino acids as nitrogen source, as described by Letort & Juillard, Development of a minimal chemically-defined medium for the exponential growth of Streptococcus thermophilus, J. Appl. Microbiol., vol. 91, p. 1023-1029, 2001. Optical density at 600 nm (OD600) of the cultures was measured using a spectrophotometer UVIKON 931 (KONTRON).
[0085]Proteins were prepared from cells grown in CDM and harve...
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