69 results about "Chemically defined medium" patented technology

The present invention relates to methods and compositions for chemically defined media for growth of mammalian cells for production of commercially useful amounts of expressed proteins.

Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.

The invention relates to the preparation and application method of a medium, which is specifically related to a medium which is used for Vero cell and a culture method of the Vero cell. The invention belongs to the biological product area. The culture method of the invention is that the adhering Vero cells are suspend and cultured in SFM serum-free medium for the domestication and adaptation; the growing pattern of the Vero cell changes from adherence growth to suspension growth; the Vero cells are further amplified in Vero amplification CHEMICALLY DEFINED MEDIUM. The method of the invention has good effects; the growing pattern of the Vero cell is transformed from adherence growth to suspension growth, which changes the growth pattern of the cell and greatly increases the yield of the cell.

Described herein is a chemostat-like continuous cell culture system that combines certain advantages of perfusion open systems and chemostat open systems to improve the culturing of mammalian cells, e.g., genetically modified cells, particularly in serum-free or chemically-defined media. The continuous culture system described herein involves culturing mammalian cells in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d−1, and a cell density of less than about 2×107 cell / mL. Also described herein is a method for producing a polypeptide and / or virus of interest in a continuous cell culture, the method comprising culturing mammalian cells expressing the polypeptide and / or virus of interest in a continuous cell culture system, which comprises a cell retention device, wherein the cell culture system has a dilution rate (D) of less than about 2 d−1, and a cell density of less than about 2×107 cell / mL; and recovering the polypeptide and / or virus of interest from medium of the cell culture system.

The invention relates to a suspension acclimatization and serum-free acclimatization method for HEK (human embryonic kidney)-293T cells, and belongs to the field of biotechnology. The suspension acclimatization and serum-free acclimatization method includes acclimatizing the HEK-293T cells adaptive to adherent culture so that the HEK-293T cells are adaptive to suspension culture in culture media with serum; and acclimatizing the HEK-293T cells adaptive to suspension culture in the culture media with the serum in a serum reducing mode so that the HEK-293T cells are adaptive to serum-free acclimatization. The suspension acclimatization and serum-free acclimatization method has the advantages that the growth status of the HEK-293T cells cultured by the suspension acclimatization and serum-free acclimatization method is stable, the HEK-293T cells are high in dispersibility and are completely adaptive to suspension culture in the serum-free culture media with certain chemical compositions, and batch production can be carried out.

The invention discloses a chemically-defined medium. A disclosed DMEM / F12 formula is taken as a basis, the concentration of various components is adjusted, certain components are added or eliminated on the basis, and the chemically-defined medium is applicable to the large-scale production of various mammalian cells. The invention further discloses application of the medium in large-scale culture of mammalian cells (CHO cells, BHK cells or hybridoma cells) and a production technology for large-scale culture of mammalian cells. The chemically-defined medium provided by the invention does not contain serum or proteins, eliminates animal ingredients and vegetable protein hydrolysate used in the conventional medium, and facilitates the product purification and production batch stability, and the chemical constituents of all the components are defined, so that the product quality is guaranteed and improved; through the adoption of the medium and the production technology, provided by the invention, the cell density is improved, and the yield of target products is increased, and the overall production cost is low.

This invention relates to the induction of hepatic differentiation by culturing induced pluripotent stem (iPS) cells in an endoderm induction medium to produce a population of anterior definitive endoderm (ADE) cells and then culturing the population of ADE cells in a hepatic induction medium to produce a population of hepatic progenitor cells, which may be optionally differentiated into hepatocytes. The endoderm induction medium is a chemically defined medium which has fibroblast growth factor activity, stimulates SMAD2 and SMAD3 mediated signalling pathways and SMAD1, SMAD5 and SMAD9 mediated signalling pathways, and inhibits phosphatidylinositol 3-kinase (PI3K) and glycogen synthase kinase 3beta (GSK3beta); and the hepatic induction medium is a chemically defined medium which stimulates SMAD2 and SMAD3 mediated signalling pathways. These methods may be useful, for example, in producing hepatocytes and hepatic progenitor cells for cell-based therapies or disease modelling.

We describe the use of chemically defined media for the fermentative production of valuable compounds on an industrial scale. Microbial strains which are suitable for fermentation on an industrial scale using a chemically defined medium include fungal, yeast and bacterial strains. Suitable strains can be obtained as wild type strains or by screening and selection after mutagenic treatment or DNA transformation.

Methods and devices for culturing human pluripotent stem cells to produce cells of the pancreatic lineage are disclosed. The methods include steps of culturing the stem cells under conditions that induce the expression of mesendoderm / primitive streak and definitive endoderm markers in a chemically defined medium including an effective amount of i) fibroblast growth factor, ii) Activin A, and iii) bone morphogenetic protein. The methods further include the steps of culturing cells under conditions favoring the formation of at least one of intact embryoid bodies and pancreatic progenitor PDX1+Ins− cells.

The invention relates to an MDBK cell acclimation suspension method and a two-stage virus production process. According to the MDBK cell acclimation suspension method, adherent MDBK cells are acclimated to adapt to serum-free suspension culture and used as host cells for producing viruses which can have cytopathic effect or sensitivity on suspension MDBK cells by two-stage culture virus inoculation; when the cells grow to 2.0-20.0*10<6> cells / mL, diluting with a production culture medium by 1-5 times is performed to reach a density range of 1.0-10.0*10<6> cells / mL, and then virus inoculation is carried out or virus inoculation is performed before dilution. The suspension MDBK cells are adaptive to suspension growth in the serum-free chemical-defined culture medium, and the two-stage virusproduction process is simple and easy in amplification; liquid changing is avoided, and high culture medium utilization efficiency is achieved; production and growth culture media can be identical ornot and can be mixed proportionally to realize cell secondary growth and viral expression promotion.

Chemically defined media compositions for the culture of eukaryotic cells are disclosed. The compositions are useful for eukaryotic cell culture in perfusion bioreactors and other vessels.

Synthetic surfaces suitable for culturing stem cell derived cardiomyocytes contain acrylate polymers formed from one or more acrylate monomers. The acrylate surfaces, in many cases, are suitable for culturing stem cell derived cardiomyocytes in chemically defined media.

The present invention relates to a chemically defined medium for eukaryotic cell culture, comprising water, at least one carbon source, one or more vitamins, one or more salts, one or more growth factors, one or more fatty acids, one or more buffer components, selenium and one or more further trace elements and its use in the culture of cancer stem cells, in particular tumorsphere culture of cancer stem cells.

This application relates to a method for differentiating pluripotent stem cells (PSCs) into cardiomyocytes. Moreover this application relates to a method for differentiating human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs) into defined cardiomyocytes based on linked steps of chemically defined medium inductions.

Synthetic surfaces capable of supporting culture of undifferentiated human embryonic stem cells in a chemically defined medium include a swellable (meth)acrylate layer and a peptide conjugated to the swellable (meth)acrylate layer. The swellable (meth)acrylate layer may be formed by polymerizing monomers in a composition that includes hydroxyethyl methacrylate, 2-carboxyehylacrylate, and tetra(ethylene glycol) dimethacrylate. The conjugated peptide may include an amino acid sequence of XaanProGlnValThrArgGlyAspValPheThrMetPro, where n is an integer from 0 to 3 and where Xaa is any amino acid. Further, disclosed herein is a swellable (meth)acrylate synthetic surface which can be sterilized by gamma irradiation.