171 results about "Bioproducts" patented technology

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

There are provided bioproducts and methods of improving production of the bioproducts from engineered microbial cells, the methods comprising: providing a fermentation broth comprising a crude carbon source; inoculating said fermentation broth with said microbial cells; and incubating the inoculated fermentation broth; wherein said bioproduct is a hydrophobic solvent immiscible with said fermentation broth, and wherein a toxic side product present in said crude carbon source is soluble in said hydrophobic solvent. Also, provided are kits for practicing the methods of improving production of bioproducts.

A hybrid photovoltaic panel-interfaced distillation with and without a hydrophobic microporous membrane distillation process is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, distilled ethanol, hot water, hot steam, saline / brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

A fractionation process for producing value-added products from a lignocellulosic biomass, comprises: a) mechanically refining the lignocellulosic biomass under mild refining conditions to form a refined biomass pulp with enhanced susceptibility to separation of hemicellulose, cellulose and lignin, and enhanced digestibility of carbohydrates in hydrolysis b) separating hemicellulose and sulfur-free high-quality lignin from cellulose in the refined biomass, and, optionally c) producing various bioproducts from the above said process.

Single-use closed bioreactors with recirculating exhaust gas that can be operated in an uncontrolled environment are reported for the manufacturing of biological products using genetically modified biological cultures that produces carbon dioxide or that requires carbon dioxide in their metabolic process.

A photo-bioreactor module adapted for stacking a plurality of such modules for producing algal bioproducts includes at least an upper and a lower light-transmitting member each having a plurality of openings. The upper and lower light-transmitting members are spaced apart from one another and at least one of the upper and lower light-transmitting members includes at least one light connection terminal for coupling in light from a light source and transmitting the light laterally. A plurality of photobioreactor conduits each extending from respective openings in the upper and lower light-transmitting member define algae containment interior spaces, wherein the plurality of photobioreactor conduits contact the upper and a lower light-transmitting members along areas of contact. The light transmitted laterally by the upper and lower light-transmitting members couples into the plurality of photobioreactor conduits along the areas of contact.

Methods for purifying glycerin contaminated with one or more lower boiling alcohols such as methanol, ethanol, straight, branched or cyclic C3-C6 alcohols, and the like. The methods are particularly useful for purifying crude glycerin phases recovered from the synthesis of biofuels. The present invention uses distillation techniques to strip alcohol contaminants from glycerin. In contrast to conventional methods that carry out distillation either under substantially anhydrous or very wet conditions, the present invention carries out distillation in the presence of a limited amount of water, e.g., from about 0.8 to about 5 parts by weight of water per 100 parts by weight of contaminated glycerin to be purified.

Methods and systems are disclosed for producing lactide, which can be used for PLA production or other valuable bioproducts. PLA is heated to undergo thermal depolymerization to recover lactide. The lactide can be used for PLA production or other valuable bioproducts.

A process for the heterotrophic or predominantly heterotrophic production of whole-celled or extracted microbial products with a high concentration of omega-3 highly unsaturated fatty acids, producible in an aerobic culture under controlled conditions using biologically pure cultures of heterotrophic single-celled fungi microorganisms of the order Thraustochytriales. The harvested whole-cell microbial product can be added to processed foods as a nutritional supplement, or to fish and animal feeds to enhance the omega-3 highly unsaturated fatty acid content of products produced from these animals. The lipids containing these fatty acids can also be extracted and used in nutritional, pharmaceutical and industrial applications.

Methods for purifying glycerin contaminated with one or more lower boiling alcohols such as methanol, ethanol, straight, branched or cyclic C3-C6 alcohols, and the like. The methods are particularly useful for purifying crude glycerin phases recovered from the synthesis of biofuels. The present invention uses distillation techniques to strip alcohol contaminants from glycerin. In contrast to conventional methods that carry out distillation either under substantially anhydrous or very wet conditions, the present invention carries out distillation in the presence of a limited amount of water, e.g., from about 0.8 to about 5 parts by weight of water per 100 parts by weight of contaminated glycerin to be purified.

A hybrid photovoltaic panel-interfaced solar-greenhouse distillation technology is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel front surface, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, hot water, hot steam, saline / brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

Methods for enzymatically isolating lignin and other bioproducts, such as fermentable sugars, from herbaceous plant materials, are described. The methods can provide improvements, such as increased product purity and reduced process energy requirements and product modifications and contamination. Systems for practicing the methods also are provided.

Provided are surprisingly beneficial osmotic stress shock methods for facilitating disruption and / or extraction of microorganisms, comprising a synergistic combination of at least one hypotonic shock and at least two hypertonic shocks, wherein the last shock is a hypertonic shock. Preferred aspects of the invention relate to facilitating disruption of a broad spectrum of microorganisms (e.g., algae, bacteria, yeast, fungus, etc.). In particular aspects the microbial cells are subjected to: a hypertonic / hypotonic / hypertonic tertiary shock; a hypotonic / hypertonic / hypertonic tertiary shock; or a hypotonic / hypertonic / hypotonic / hypertonic quaternary shock. Particular aspects further comprise disrupting and / or extracting of the shocked microbial cells, and isolating a cellular constituent or bioproduct (e.g., biofuel, biocrude, bioenergy, biogas, biodiesel, bioethanol, biogasoline, pharmaceuticals, nutraceuticals, food, vitamins, feedstock, dyes, colorants, sulfur, fertilizer, bioplastic) therefrom. In particular preferred aspects, facilitation of algae disruption and / or extraction is provided and in certain embodiments, isolation of at least one of lipid, oil, and triacylglycerol is enhanced.

The present invention relates to genetically engineered organisms, especially microorganisms such as bacteria and yeasts, for the production of added value bio-products such as specialty saccharide, activated saccharide, nucleoside, glycoside, glycolipid or glycoprotein. More specifically, the present invention relates to host cells that are metabolically engineered so that they can produce said valuable specialty products in large quantities and at a high rate by bypassing classical technical problems that occur in biocatalytical or fermentative production processes.

The invention provides a method of making mangel immune polysaccharides and yeasts extract, belonging to the technique field of preparing biological products, such as polysaccharides, by yeasts. Said method includes the following steps: brewers' yeast residue bitter-removing, foreign substance-removing, brewers' yeast autolysis, brewers' yeast complex enzymolysis and deactivation, segregation of polysaccharides and protein hydrolysate liquid, blink spray drying of mangel immune polysaccharides and yeasts extract, etc; it is characterized on: high yield, good quality, low cost, and could achieve the aim of preparing mangel immune polysaccharides and yeasts extract at the same time from identical yeast material, enhance the availability ratio of yeasts, avoid pollution to the environment in traditional method as a result of simply preparing one certain product and discharging other assistant liquid.

Disclosed is a method of detecting bioproducts using Localized Surface Plasmon Resonance (LSPR) of gold nanoparticles, which can diagnose bioproducts based on changes in the maximum wavelength occurred by an antigen-antibody reaction after immobilization of the gold nanoparticles onto a glass panel. A sensor using such method exhibits high sensitivity, is low in price, and makes quick diagnosis possible, thereby being applicable to various biological fields associated with environmental contaminants, pathogens and the like, as well as diagnosis of diseases. Further, it provides a technology for manufacturing a sensor having higher sensitivity, low price and quick performance, as compared to conventional methods using SPR.

A plant nutrient is prepared by chelating micromolecular collagen peptide and middle elements and / or microelements, wherein, the molecular weight of the micromolecular collagen peptide is 1000-5000, preferably 1000-3000. The micromolecular collagen peptide takes fishery wastes as a collagen source, the initial molecular weight of the collagen peptide is 2300000-2500000 units, and an enzymatic directional shear technology is adopted to perform enzymolysis on the collagen peptide to form the micromolecular collagen peptide. The plant nutrient provided by the invention is a new-generation purely natural green agricultural resource and belongs to an efficient and all-nutrition collagen peptide preparation; and the plant nutrient has the advantages of no toxicity, no residue and no pollution of the traditional nuisance-free biologics, and can improve the crop autoimmunity and the crop disease-resistance, and reduce pesticide consumption. The micromolecular collagen peptide has the functions of promoting cell proliferation and maintaining the cell division, and the micromolecular collagen peptide which is chelated with the elements can provide pure natural nutrition to crops such as fruit, vegetables, grain and cotton and the like to increase the crop yield and improve agricultural product quality.

Particularly applicable to the implementation of sustainability requirements concerning on the promotion of the use of bioproduct from renewable sources, through the system and method described is possible to ensure that the origin of raw materials is sustainable (according to a previously defined sustainability requirements), avoiding travel to the area of interest, thus saving time and economic costs and preventing errors and fraud. More specifically, the system and method object of the invention are particularly applicable for identifying those areas that comply with said sustainability requirements. Said sustainability requirements state that raw material intended for bioproduct shall not be made from lands with a high biodiversity, high carbon stock or peatlands, and bearing in mind additionally the land use requirement.

A hybrid photovoltaic panel-interfaced solar-greenhouse distillation technology is disclosed that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermal contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Use of this invention can provide a series of distillation-related products such as freshwater, distilled water, hot water, hot steam, sea salts, saline / brine products, and / or harvest biofuels and bioproducts such as ethanol from renewable resources while co-generating solar electricity.

A hybrid photovoltaic panel-interfaced distillation with and without a hydrophobic microporous membrane distillation process is provided that is capable of utilizing solar waste heat to perform liquid distillation while co-generating solar electricity. Solar waste heat co-generated at a photovoltaic panel is effectively utilized by in situ distillation liquid as an immediate heat sink in thermo contact with the photovoltaic panel, thus providing beneficial cooling of the photovoltaic panel and co-making of distillation products while generating electricity with significant improvement on total-process solar energy utilization efficiency. Its enabled beneficial utilization of waste heat can provide a series of distillation-related products such as: freshwater, sea salts, distilled water, distilled ethanol, hot water, hot steam, saline / brine products, and brine photobiological cultures for production of advanced biofuels and bioproducts, in addition to solar electricity.

Microorganisms and bioprocesses are provided that convert gaseous C1 containing substrates, such as syngas, producer gas, and renewable H2 combined with CO2, into nutritional and other useful bioproducts.

An assay technique for label-free, highly parallel, qualitative and quantitative detection of specific cell populations in a sample and for assessing cell functional status, cell-cell interactions and cellular responses to drugs, environmental toxins, bacteria, viruses and other factors that may affect cell function. The technique includes a) creating a first array of binding regions in a predetermined spatial pattern on a sensor surface capable of specifically binding the cells to be assayed; b) creating a second set of binding regions in specific spatial patterns relative to the first set designed to efficiently capture potential secreted or released products from cells captured on the first set of binding regions; c) contacting the sensor surface with the sample, and d) simultaneously monitoring the optical properties of all the binding regions of the sensor surface to determine the presence and concentration of specific cell populations in the sample and their functional status by detecting released or secreted bioproducts.

A method for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a Stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage.

Provided are compositions and methods for sustainable cultivation of algae for biomass, biofuel and bioproduct production, preferably with minimal addition of exogenous nutrients, comprising co-cultivating at least one algal species with at least one aerobic bacterial species and at least one diazotroph (or, in certain embodiments, cultivation of at least one algal species with at least one diazotroph) under continuous sustainable symbiotic conditions, wherein a significant proportion of the macronutrients derive from endogenous decomposed algal and bacterial cells. Certain aspects provide continuous symbiotic diazotroph-attenuated nitrogen stress co-cultivation, wherein a continuous, balanced attenuated nitrogen-stress response provides for adequate sustained algal growth, while yet preserving advantages of algal nitrogen stress responses for algal bioproduct production. Preferred aspects provide for enhanced algal production of at least one of: lipids; triacylglycerols (TAGs); percentage of lips as TAGs; and percentage of saturated and mono-saturated fatty acids relative to polyunsaturated fatty acids (PUFAs) in TAGs.

An apparatus and method for recovering bioproducts from a feed solution. In one embodiment, the apparatus includes a module housing, a membrane means disposed in the housing for filtering the bioproducts from the feed solution wherein a portion of the membrane means is coated with a polymeric coating, and an adsorbent bed disposed in the housing for retaining the bioproducts which permeate through the membrane, wherein the apparatus is adapted to allow fractionation and purification of the retained bioproducts from the bed by elution.

A method for the production of alcohol and other bioproducts hemicelluloses extracted from biomass prior to thermal conversion of the biomass to energy. The process can be integrated with the host plant process to minimize the energy loss from extracting hemicelluloses. Also disclosed is a Stepwise enzymatic break down of cellulose fibers from a pulping operation which is performed with the redeployment of equipment and vessels contained within typical existing pulp and paper manufacturing mills. The preferred feedstock is highly delignified pulp from acid or alkaline pulping process or from bleaching stage.

An automated system for monitoring and treating pests in a crop field, comprising at least one trap for monitoring and identifying pests, at least one UA V containing at least one chemical or biological products; a home base for parking or storing said at least one UA V when they are not operating; at least one database server; and equipment for communicating with said at least one trap, said at least one home base, said at least one UA V and said at least one database server.

Particularly applicable to the implementation of sustainability requirements concerning on the promotion of the use of bioproduct from renewable sources, through the system and method described is possible to ensure that the origin of raw materials is sustainable (according to a previously defined sustainability requirements), avoiding travel to the area of interest, thus saving time and economic costs and preventing errors and fraud. More specifically, the system and method object of the invention are particularly applicable for identifying those areas that comply with said sustainability requirements. Said sustainability requirements state that raw material intended for bioproduct production shall not be made from lands with a high biodiversity, high carbon stock or peatlands, and bearing in mind additionally the land use requirement.